PE20040166A1 - Celulas hospedantes que tienen propiedades mejoradas de supervivencia celular y metodos para generar estas celulas - Google Patents

Celulas hospedantes que tienen propiedades mejoradas de supervivencia celular y metodos para generar estas celulas

Info

Publication number
PE20040166A1
PE20040166A1 PE2003000306A PE2003000306A PE20040166A1 PE 20040166 A1 PE20040166 A1 PE 20040166A1 PE 2003000306 A PE2003000306 A PE 2003000306A PE 2003000306 A PE2003000306 A PE 2003000306A PE 20040166 A1 PE20040166 A1 PE 20040166A1
Authority
PE
Peru
Prior art keywords
cho
cell
cells
host cells
methods
Prior art date
Application number
PE2003000306A
Other languages
English (en)
Inventor
Barbara Enenkel
Heiko Meents
Martin Fussenegger
Original Assignee
Boehringer Ingelheim Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma filed Critical Boehringer Ingelheim Pharma
Publication of PE20040166A1 publication Critical patent/PE20040166A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE REFIERE A UNA CELULA HOSPEDANTES DE HAMSTER GENETICAMENTE MODIFICADA MEDIANTE LA INTRODUCCION DE SECUENCIAS DE ACIDOS NUCLEICOS QUE CODIFICAN UN GEN ANTI-APOPTOSIS, UN GEN MARCADOR AMPLIFICABLE Y SELECCIONABLE Y AL MENOS UN GEN DE INTERES. LA CELULA HOSPEDANTE ES UNA CELULA DE OVARIO DE HAMSTER CHINO (CHO) O UNA CELULA DE RINON DE CRIA DE HAMSTER (BHK) O SELECCIONADAS DE CHO-DG44, CHO-K1, CHO PRO-5, V79, ENTRE OTROS O LA PROGENIE DE CUALQUIERA DE ESTAS LINEAS CELULARES. EL GEN MARCADOR CODIFICA LA DIHIDROFOLATOREDUCTASA (DHFR), GLUTAMINA-SINTETASA, CAD, ADENOSINA-DESAMINASA, ADENILATO-DESAMINASA, UMP-SINTETASA ENTRE OTROS. SE REFIERE ADEMAS A METODOS QUE MODULAN LOS GENES ACTIVOS ANTI-APOPTOSIS EN CELULAS HOSPEDANTES Y CELULAS HOSPEDANTES QUE MUESTRAN UNA VIABILIDAD CELULAR AUMENTADA INHIBIENDO LA MUERTE CELULAR PROGRAMADA
PE2003000306A 2002-03-28 2003-03-26 Celulas hospedantes que tienen propiedades mejoradas de supervivencia celular y metodos para generar estas celulas PE20040166A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02007144A EP1348758A1 (en) 2002-03-28 2002-03-28 Host cells having improved cell survival properties and methods to generate such cells

Publications (1)

Publication Number Publication Date
PE20040166A1 true PE20040166A1 (es) 2004-05-20

Family

ID=27798823

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2003000306A PE20040166A1 (es) 2002-03-28 2003-03-26 Celulas hospedantes que tienen propiedades mejoradas de supervivencia celular y metodos para generar estas celulas

Country Status (19)

Country Link
EP (2) EP1348758A1 (es)
JP (1) JP2005521406A (es)
KR (1) KR101080626B1 (es)
CN (1) CN1646683A (es)
AR (1) AR039185A1 (es)
AT (1) ATE459705T1 (es)
AU (1) AU2003222777A1 (es)
BR (1) BR0308810A (es)
CA (1) CA2480684C (es)
DE (1) DE60331529D1 (es)
DK (1) DK1490482T3 (es)
ES (1) ES2339760T3 (es)
IL (1) IL163599A0 (es)
MX (1) MXPA04009336A (es)
NZ (1) NZ536077A (es)
PE (1) PE20040166A1 (es)
TW (2) TWI332991B (es)
UY (1) UY27739A1 (es)
WO (1) WO2003083093A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1414948B1 (en) 2001-07-10 2009-09-16 Biogen Idec Inc. Inhibition of apoptosis process and improvement of cell performance
KR100454016B1 (ko) * 2002-01-05 2004-10-26 한국과학기술원 항예정사 유전자로 형질전환되고 ｄｈｆｒ 유전자가결핍된 신규한 ｃｈｏ 세포주, 그의 제조 방법 및 상기형질전환된 ｃｈｏ 숙주 세포를 이용한 목적단백질의 생산방법
WO2004003151A2 (en) * 2002-06-26 2004-01-08 Biogen Idec Ma Inc. Protein production methods and modified cells for use therein
WO2004062554A2 (en) * 2003-01-08 2004-07-29 Nsgene A/S Enhancement of dopaminergic neuron generation and survital
EP1676911B1 (en) * 2003-10-24 2010-04-21 Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute Novel recombinant animal cells with high protein production, method of constructing the same and method of mass protein production using the same
US7531327B2 (en) * 2004-07-23 2009-05-12 Immunomedics, Inc. Methods and compositions for increasing longevity and protein yield from a cell culture
US7608425B2 (en) 2004-07-23 2009-10-27 Immunomedics, Inc. Methods for protein expression in mammalian cells in serum-free medium
US7537930B2 (en) 2004-07-23 2009-05-26 Immunomedics, Inc. Mammalian cell lines for increasing longevity and protein yield from a cell culture
EP1953222A1 (en) * 2007-01-24 2008-08-06 Boehringer Ingelheim Pharma GmbH & Co. KG Improvement of cell growth
EP2209891A1 (en) * 2007-11-13 2010-07-28 Boehringer Ingelheim Pharma GmbH & Co. KG Improving the secretory capacity in host cells
UA106047C2 (uk) * 2008-06-13 2014-07-25 Сентокор Орто Байотек Інк. Спосіб отримання високої щільності життєздатних клітин в культурі клітин ссавців
US8962312B2 (en) 2008-07-23 2015-02-24 Boehringer Ingelheim Pharma Gmbh & Co. Kg Production host cell lines
EP3785767A1 (en) * 2011-12-02 2021-03-03 Fate Therapeutics, Inc. Enhanced stem cell composition
CN104673754B (zh) * 2015-02-14 2018-03-02 百泰生物药业有限公司 重组骨髓瘤细胞克隆的筛选方法及由此获得的抗体分子
JP7214962B2 (ja) * 2018-01-17 2023-01-31 東ソー株式会社 アルカリホスファターゼ高発現動物細胞
CN116064398A (zh) * 2022-07-04 2023-05-05 上海倍谙基生物科技有限公司 定点同步敲入和敲除基因的抗凋亡细胞改造方法及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143291A (en) * 1995-05-04 2000-11-07 Us Navy Methods for modulating T cell survival by modulating bcl-XL protein level
WO2000075291A2 (en) * 1999-06-08 2000-12-14 Novartis Ag Cells with enforced expression of bcl-xl

Also Published As

Publication number Publication date
DK1490482T3 (da) 2010-07-05
CN1646683A (zh) 2005-07-27
CA2480684A1 (en) 2003-10-09
KR101080626B1 (ko) 2011-11-08
NZ536077A (en) 2006-04-28
JP2005521406A (ja) 2005-07-21
KR20040099373A (ko) 2004-11-26
CA2480684C (en) 2011-01-25
BR0308810A (pt) 2005-06-28
UY27739A1 (es) 2003-10-31
EP1490482A1 (en) 2004-12-29
TWI332990B (en) 2010-11-11
ATE459705T1 (de) 2010-03-15
AR039185A1 (es) 2005-02-09
EP1490482B1 (en) 2010-03-03
TWI332991B (en) 2010-11-11
TW200307044A (en) 2003-12-01
IL163599A0 (en) 2005-12-18
EP1348758A1 (en) 2003-10-01
MXPA04009336A (es) 2005-01-25
TW201000633A (en) 2010-01-01
DE60331529D1 (de) 2010-04-15
ES2339760T3 (es) 2010-05-25
WO2003083093A1 (en) 2003-10-09
AU2003222777A1 (en) 2003-10-13

Similar Documents

Publication Publication Date Title
PE20040166A1 (es) Celulas hospedantes que tienen propiedades mejoradas de supervivencia celular y metodos para generar estas celulas
CY1120496T1 (el) Μεθοδος για τη δημιουργια ενος ζωου ομοζυγου για μια γενετικη τροποποιηση
CY1113964T1 (el) Μεθοδοι τροποποιησης ευκαρυωτικων κυτταρων
Underwood et al. Ecologically relevant dispersal of corals on isolated reefs: implications for managing resilience
AR114014A1 (es) Sistema de edición de bases y método basado en proteína cpf1
HN2008000346A (es) Secuencias de nucleotidos que codifican proteinas insecticidas
GT200300046A (es) Indolil-urea derivados de tienopiridinas utiles como agentes antiangiogenicos y procedimientos para su uso
Riley et al. A laboratory experiment to determine the dispersal response of Atlantic salmon (Salmo salar) fry to street light intensity
DE60327804D1 (de) Alanin-2,3-aminomutase
DE60334112D1 (de) Optische fluoreszenzmarkierungen im nanomassstab und verwendungen davon
DE60326940D1 (de) Vorrichtung zum erzeugen ultravioletter strahlung
NI200700278A (es) Anticuerpos anti-il-6, composiciones, métodos y usos.
GT200100248A (es) Sistema estabilizador para estabilizar polimeros conteniendo halogeno.
MX2021014528A (es) Metodos y composiciones para generar alelos dominantes usando edicion del genoma.
Cabezas et al. Deep under the sea: unraveling the evolutionary history of the deep-sea squat lobster Paramunida (Decapoda, Munididae)
Le Reste et al. Signaling the unfolded protein response in primary brain cancers
Nazir et al. Stock discrimination of Sperata aor from river Ganga using microsatellite markers: implications for conservation and management
Brandão et al. Genetic diversity of neotropical tree Myrcia splendens (Myrtaceae) in a fragment–corridor system in the Atlantic rainforest
RICHTER‐BOIX et al. Genetic analysis of differentiation among breeding ponds reveals a candidate gene for local adaptation in Rana arvalis
ATE408018T1 (de) Stress-resistenz als selektierbarer marker in transgenen pflanzen
Kusuma et al. Close genetic connectivity of soft coral Sarcophyton trocheliophorum in Indonesia and its implication for marine protected area
Chew et al. Review of the effect of reduced levels of background radiation on living organisms
GB0720502D0 (en) Markers of matrix gene expression and cellular differentiation in chondrocytes
Buchanan Reply from KL Buchanan
COBO‐SIMÓN et al. Testing the effectiveness of conservation management within biosphere reserves: the case of the Mexican deer mouse (Peromyscus mexicanus) as a bioindicator

Legal Events

Date Code Title Description
FC Refusal