PL1907553T3 - Zmodyfikowane miejsca rekombinacji FRT oraz sposoby ich zastosowania - Google Patents

Zmodyfikowane miejsca rekombinacji FRT oraz sposoby ich zastosowania

Info

Publication number
PL1907553T3
PL1907553T3 PL06787308T PL06787308T PL1907553T3 PL 1907553 T3 PL1907553 T3 PL 1907553T3 PL 06787308 T PL06787308 T PL 06787308T PL 06787308 T PL06787308 T PL 06787308T PL 1907553 T3 PL1907553 T3 PL 1907553T3
Authority
PL
Poland
Prior art keywords
methods
recombination sites
frt recombination
modified frt
modified
Prior art date
Application number
PL06787308T
Other languages
English (en)
Inventor
Yumin Tao
Dennis Bidney
William J Gordon-Kamm
Leszek A Lyznik
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of PL1907553T3 publication Critical patent/PL1907553T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL06787308T 2005-07-18 2006-07-14 Zmodyfikowane miejsca rekombinacji FRT oraz sposoby ich zastosowania PL1907553T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US70022505P 2005-07-18 2005-07-18
EP06787308A EP1907553B1 (en) 2005-07-18 2006-07-14 Modified frt recombination sites and methods of use
PCT/US2006/027380 WO2007011733A2 (en) 2005-07-18 2006-07-14 Modified frt recombination sites and methods of use

Publications (1)

Publication Number Publication Date
PL1907553T3 true PL1907553T3 (pl) 2013-02-28

Family

ID=37669396

Family Applications (1)

Application Number Title Priority Date Filing Date
PL06787308T PL1907553T3 (pl) 2005-07-18 2006-07-14 Zmodyfikowane miejsca rekombinacji FRT oraz sposoby ich zastosowania

Country Status (8)

Country Link
US (10) US20070015195A1 (pl)
EP (1) EP1907553B1 (pl)
BR (1) BRPI0612862A2 (pl)
CA (1) CA2615797C (pl)
ES (1) ES2390132T3 (pl)
MX (1) MX2008000764A (pl)
PL (1) PL1907553T3 (pl)
WO (1) WO2007011733A2 (pl)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7578598B2 (en) * 2006-11-13 2009-08-25 Black & Decker Inc. Battery charging work light
CA2615797C (en) * 2005-07-18 2014-04-29 Pioneer Hi-Bred International, Inc. Modified frt recombination sites and methods of use
PL2018435T3 (pl) 2006-05-17 2012-11-30 Pioneer Hi Bred Int Sztuczne minichromosomy roślinne
US8293533B2 (en) 2008-12-19 2012-10-23 E.I. Du Pont De Nemours And Company Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
CA2793596A1 (en) 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted polynucleotide modification
CA2838330C (en) 2010-08-23 2021-01-26 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
US9207237B2 (en) 2010-08-23 2015-12-08 President And Fellows Of Harvard College Systems, methods, and workflows for optogenetics analysis
AR085533A1 (es) 2011-03-23 2013-10-09 Pioneer Hi Bred Int Metodos de produccion de un locus de rasgos transgenicos complejo
BR112014002622A2 (pt) 2011-08-03 2019-09-24 Du Pont método para introduzir no genoma de uma célula vegetal um sítio alvo para a integração específica de sítio, célula vegetal, parte de planta, planta ou semente, método para integrar um polinucleotídeo de interesse em um sítio alvo no genoma de uma célula vegetal
CN102533744A (zh) * 2011-12-29 2012-07-04 中国科学院深圳先进技术研究院 基因表达盒及含有该基因表达盒的重组表达载体
AR089793A1 (es) 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
US20140173775A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic plants
US10793867B2 (en) * 2013-03-15 2020-10-06 Monsanto Technology, Llc Methods for targeted transgene-integration using custom site-specific DNA recombinases
WO2015017510A1 (en) 2013-07-31 2015-02-05 E. I. Du Pont De Nemours And Company Modification of soybean seed composition to enhance feed, food and other industrial applications of soybean products
CN106133138A (zh) 2013-08-22 2016-11-16 先锋国际良种公司 使用引导多核苷酸/cas内切核酸酶系统的基因组修饰及其使用方法
US10233456B2 (en) 2014-01-30 2019-03-19 The Board Of Trustees Of The University Of Arkansas Method, vectors, cells, seeds and kits for stacking genes into a single genomic site
CN103882530B (zh) * 2014-03-26 2016-02-24 清华大学 用随机序列标记质粒对dna片段进行高通量两端测序的方法
AR100159A1 (es) 2014-04-22 2016-09-14 Du Pont Genes de anhidrasa carbónica plastídica para el incremento de aceite en semillas con expresión incrementada de dgat
EP3158338B1 (en) 2014-06-18 2020-03-11 President and Fellows of Harvard College Optogenetic probes for measuring membrane potential
EP3166391A1 (en) 2014-07-11 2017-05-17 E. I. du Pont de Nemours and Company Compositions and methods for producing plants resistant to glyphosate herbicide
KR102227771B1 (ko) * 2014-08-25 2021-03-16 삼성전자주식회사 나노구조 반도체 발광소자
BR112017003757A2 (pt) 2014-09-12 2017-12-26 Du Pont ?plantas de milho, partes de planta de milho ou sementes de milho?
WO2016050917A1 (en) * 2014-10-01 2016-04-07 Dsm Ip Assets B.V. Methods for genomic modification
WO2016061197A1 (en) 2014-10-16 2016-04-21 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides having improved activity spectrum and uses thereof
WO2016115333A1 (en) 2015-01-15 2016-07-21 President And Fellows Of Harvard College Optical selection of cells
US11492632B2 (en) 2015-03-19 2022-11-08 Pioneer Hi-Bred International, Inc. Methods and compositions for accelerated trait introgression
BR112017017260A2 (pt) 2015-03-27 2018-04-17 Du Pont construções de dna, vetor, célula, plantas, semente, método de expressão de rna e método de modificação de local alvo
US11236347B2 (en) 2015-08-28 2022-02-01 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
US20180258438A1 (en) 2015-11-06 2018-09-13 Pioneer Hi-Bred International, Inc. Generation of complex trait loci in soybean and methods of use
US10374872B2 (en) 2016-05-24 2019-08-06 Apstra, Inc. Configuring system resources for different reference architectures
GB201712678D0 (en) 2017-08-07 2017-09-20 Glaxosmithkline Biologicals Sa Process for the manipulation of nucleic acids
WO2019089402A1 (en) 2017-10-30 2019-05-09 Dow Agrosciences Llc Plant promoter for transgene expression
KR20200124702A (ko) 2018-02-23 2020-11-03 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 신규한 cas9 오르소로그
CN113166744B (zh) 2018-12-14 2025-02-07 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
EP4222165A2 (en) 2020-09-30 2023-08-09 Pioneer Hi-Bred International, Inc. Rapid transformation of monocot leaf explants
EP4405375A1 (en) 2021-09-23 2024-07-31 President and Fellows of Harvard College Genetically encoded voltage indicators and uses thereof

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
WO1993001283A1 (en) 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
US5635381A (en) 1992-02-26 1997-06-03 Mogen International Nv Agrobacterium bacteria capable of site-specific recombination
AU670316B2 (en) 1992-07-27 1996-07-11 Pioneer Hi-Bred International, Inc. An improved method of (agrobacterium)-mediated transformation of cultured soybean cells
ATE199392T1 (de) * 1992-12-04 2001-03-15 Medical Res Council Multivalente und multispezifische bindungsproteine, deren herstellung und verwendung
AU6162294A (en) 1993-01-13 1994-08-15 Pioneer Hi-Bred International, Inc. High lysine derivatives of alpha-hordothionin
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE4416784A1 (de) 1994-05-09 1995-11-30 Soft Gene Gmbh Methode zur Anreicherung von Zellen, die durch ballistischen Transfer modifiziert wurden
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
BR9609200A (pt) 1995-06-02 1999-05-11 Pioneer Hi Bred Int Derivados de alta treonina de alpha-hordotionina
CN1192239A (zh) 1995-06-02 1998-09-02 先锋高级育种国际公司 α-hordothionin的高甲硫氨酸衍生物
US6051409A (en) * 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
CA2230966C (en) 1995-09-25 2010-08-24 Novartis Ag Improved integration of exogenous dna delivered to plant cells
AU716781B2 (en) 1995-10-13 2000-03-09 Purdue Research Foundation Method for the production of hybrid plants
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
US5753477A (en) 1996-03-19 1998-05-19 University Technology Corporation Magneto-biolistic methods
US5850016A (en) 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
AUPN903196A0 (en) 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
AU3495297A (en) 1996-07-08 1998-02-02 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US7135608B1 (en) 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
US6114600A (en) 1997-10-31 2000-09-05 The United States Of America As Represented By The Secretary Of Agriculture Resolution of complex integration patterns to obtain single copy transgenes
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型loxP配列とその応用
PT1034262E (pt) * 1997-11-18 2005-10-31 Pioneer Hi Bred Int Composicoes e metodos para a modificacao genetica de plantas
US6720475B1 (en) * 1997-11-18 2004-04-13 Pioneer Hi-Bred International, Inc. Modified nucleic acid sequence encoding FLP recombinase
CA2309719A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Targeted manipulation of genes in plants
AU745960C (en) 1997-11-18 2003-09-18 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign DNA into eukaryoticgenomes
US7102055B1 (en) * 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
NZ504510A (en) 1997-11-18 2002-10-25 Pioneer Hi Bred Int Methods and compositions for increasing efficiency of excision of a viral replicon from T-DNA that is transferred to a plant by agroinfection
US5929301A (en) * 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
EP0939120A1 (en) * 1998-02-27 1999-09-01 Gesellschaft für biotechnologische Forschung mbH (GBF) Method for marker-free repetitive DNA expression cassette exchange in the genome of cells or parts of cells
WO1999055851A2 (en) 1998-04-28 1999-11-04 Novartis Ag Site-directed transformation of plants
US6781032B1 (en) 1998-08-14 2004-08-24 The Regents Of The University Of California Homologous recombination in plants
JP4478333B2 (ja) 1998-10-12 2010-06-09 大日本住友製薬株式会社 リコンビナーゼ発現細胞
WO2000046385A1 (en) 1999-02-03 2000-08-10 The Children's Medical Center Corporation Gene repair involving in vivo excision of targeting dna
US6410317B1 (en) * 1999-07-14 2002-06-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
US6746870B1 (en) * 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
DE60044142D1 (de) 1999-07-23 2010-05-20 Univ California Dna-rekombination in eukaryotischen zellen mittels des rekombinationszentrums vom bakteriophag phic31
WO2001011058A1 (en) 1999-08-09 2001-02-15 Monsanto Technology Llc Novel cloning methods and vectors
JP4787440B2 (ja) * 1999-09-30 2011-10-05 泉 斎藤 変異型frt配列を含有してなるdna
US6849778B1 (en) * 1999-10-15 2005-02-01 Calgene Llc Methods and vectors for site-specific recombination in plant cell plastids
US7126041B1 (en) * 1999-12-10 2006-10-24 North Carolina State Unversity High efficiency gene targeting in plants
US20020023278A1 (en) * 2000-05-08 2002-02-21 Lyznik Leszek Alexander Genetic transformation in plants using site-specific recombination and wide hybridization
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AU7752001A (en) 2000-06-28 2002-01-08 Sungene Gmbh And Co Kgaa Binary vectors for improved transformation of plant systems
BR0112633A (pt) * 2000-07-21 2003-09-16 Us Agriculture Método para substituir, translocar e empilhar dna em genomas eucarióticos
ES2394877T3 (es) * 2000-08-14 2013-02-06 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Recombinación homóloga mejorada mediada por proteínas de recombinación de lambda
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AU2002219841A1 (en) * 2000-11-16 2002-05-27 Cornell Research Foundation Inc. Vectors for conditional gene inactivation
US6696278B1 (en) * 2001-02-26 2004-02-24 Stratagene Method for transfer of DNA segments
US7074611B2 (en) * 2001-04-27 2006-07-11 Gie-Cerbn, Centre Europeen De Recherche En Biologie Et En Medecine (Gie) Method for the stable inversion of DNA sequence by site-specific recombination and DNA vectors and transgenic cells thereof
DE10131786A1 (de) 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
US7041648B2 (en) 2001-08-03 2006-05-09 Sigma-Tau Industrie Farmaceutiche Riunite S.P.A. Compositions and methods for treating female fertility
US7566815B2 (en) * 2001-09-03 2009-07-28 National Institute of Agrobiological Sciences and National Agricultural and Bio-oriented Research Organization Control of plant flowering time by regulation of phytochrome c expression
EP1427828B1 (en) 2001-09-14 2010-04-28 Cellectis Random integration of a polynucleotide after in vivo linearization
WO2003054189A2 (de) 2001-12-20 2003-07-03 Sungene Gmbh & Co. Kgaa Verfahren zur transformation von pflanzlichen plastiden
US20030167526A1 (en) 2002-01-14 2003-09-04 Pioneer Hi-Bred International Inc. Compositions and methods for identifying transformed cells
JP2005521400A (ja) * 2002-03-29 2005-07-21 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト 植物におけるラムダインテグラーゼ媒介型組換え
US7238854B2 (en) * 2002-04-11 2007-07-03 E. I. Du Pont De Nemours And Company Method of controlling site-specific recombination
AU2003247696A1 (en) * 2002-07-01 2004-01-19 E.I. Du Pont De Nemours And Company Method of controlling gene silencing using site-specific recombination
US20040137624A1 (en) * 2002-12-27 2004-07-15 Lowe Brenda A. Methods of site-directed transformation
PT2025756E (pt) 2003-11-18 2011-09-28 Bayer Bioscience Nv Inserção direccionada de adn em plantas
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
US20060288444A1 (en) 2004-08-13 2006-12-21 Mccarroll Robert Soybean polymorphisms and methods of genotyping
PT1885176T (pt) 2005-05-27 2016-11-28 Monsanto Technology Llc Evento mon89788 de soja e métodos para a sua deteção
US20070016985A1 (en) 2005-07-18 2007-01-18 Pioneer Hi-Bred International, Inc. Particle Preparation for Direct-Delivery Transformation
CA2615797C (en) 2005-07-18 2014-04-29 Pioneer Hi-Bred International, Inc. Modified frt recombination sites and methods of use
WO2007050715A2 (en) 2005-10-26 2007-05-03 Integrated Plant Genetics, Inc. Compositions and methods for safe delivery of biologically-active plant transformation agents using non-fibrous silicon carbide powder
US20080083042A1 (en) 2006-08-14 2008-04-03 David Butruille Maize polymorphisms and methods of genotyping
CL2008001865A1 (es) 2007-06-22 2008-12-26 Monsanto Technology Llc Método para identificar una muestra de germoplasma vegetal con un genotipo que modula el rendimiento de una caracteristica, y célula vegetal que contiene al menos una región genómatica identificada para modular el rendiemiento de transgenes.

Also Published As

Publication number Publication date
US8318493B2 (en) 2012-11-27
BRPI0612862A2 (pt) 2010-11-30
US20130052739A1 (en) 2013-02-28
ES2390132T3 (es) 2012-11-06
US20100173801A1 (en) 2010-07-08
CA2615797C (en) 2014-04-29
US20150044771A1 (en) 2015-02-12
US9234194B2 (en) 2016-01-12
US8586361B2 (en) 2013-11-19
US20170369893A1 (en) 2017-12-28
US7736897B2 (en) 2010-06-15
US20160108412A1 (en) 2016-04-21
WO2007011733A3 (en) 2007-09-07
US9777284B2 (en) 2017-10-03
WO2007011733A2 (en) 2007-01-25
US8900869B2 (en) 2014-12-02
US11225668B2 (en) 2022-01-18
MX2008000764A (es) 2008-03-24
EP1907553B1 (en) 2012-08-22
EP1907553A2 (en) 2008-04-09
US20110047655A1 (en) 2011-02-24
CA2615797A1 (en) 2007-01-25
US20100192263A1 (en) 2010-07-29
US20080047031A1 (en) 2008-02-21
US20070015195A1 (en) 2007-01-18
US20190194673A1 (en) 2019-06-27

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