PL2227549T3 - System selekcji do hodowli komórek eukariotycznych oparty na genie związanego z błoną receptora folianowego - Google Patents

System selekcji do hodowli komórek eukariotycznych oparty na genie związanego z błoną receptora folianowego

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Publication number
PL2227549T3
PL2227549T3 PL08865304T PL08865304T PL2227549T3 PL 2227549 T3 PL2227549 T3 PL 2227549T3 PL 08865304 T PL08865304 T PL 08865304T PL 08865304 T PL08865304 T PL 08865304T PL 2227549 T3 PL2227549 T3 PL 2227549T3
Authority
PL
Poland
Prior art keywords
membrane
cell culture
eukaryotic cell
selection system
receptor gene
Prior art date
Application number
PL08865304T
Other languages
English (en)
Inventor
Yehuda G Assaraf
Thomas Jostock
Hans-Peter Knopf
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of PL2227549T3 publication Critical patent/PL2227549T3/pl

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1079Screening libraries by altering the phenotype or phenotypic trait of the host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
PL08865304T 2007-12-21 2008-12-19 System selekcji do hodowli komórek eukariotycznych oparty na genie związanego z błoną receptora folianowego PL2227549T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP07150326 2007-12-21
EP08865304.3A EP2227549B1 (en) 2007-12-21 2008-12-19 Selection system for eukaryotic cell culture based on a membrane-bound folate receptor gene
PCT/EP2008/068046 WO2009080759A1 (en) 2007-12-21 2008-12-19 Organic compounds

Publications (1)

Publication Number Publication Date
PL2227549T3 true PL2227549T3 (pl) 2015-12-31

Family

ID=39560901

Family Applications (1)

Application Number Title Priority Date Filing Date
PL08865304T PL2227549T3 (pl) 2007-12-21 2008-12-19 System selekcji do hodowli komórek eukariotycznych oparty na genie związanego z błoną receptora folianowego

Country Status (15)

Country Link
US (5) US20100330572A1 (pl)
EP (1) EP2227549B1 (pl)
JP (2) JP5660900B2 (pl)
KR (1) KR101706390B1 (pl)
CN (2) CN101903526B (pl)
AU (1) AU2008340017B2 (pl)
BR (1) BRPI0821401A2 (pl)
CA (1) CA2709503C (pl)
DK (1) DK2227549T3 (pl)
ES (1) ES2546086T3 (pl)
IL (1) IL206233A (pl)
PL (1) PL2227549T3 (pl)
PT (1) PT2227549E (pl)
RU (1) RU2502802C2 (pl)
WO (1) WO2009080759A1 (pl)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5801207B2 (ja) 2009-02-27 2015-10-28 ノバルティス アーゲー 2種類の選択マーカーを含む発現ベクター系
TWI622402B (zh) 2010-02-24 2018-05-01 免疫遺傳股份有限公司 葉酸受體1抗體類和免疫共軛物類及彼等之用途
BR112013025415B1 (pt) 2011-04-01 2021-03-16 Immunogen, Inc uso de um imunoconjugado antirreceptor de folato 1 (folr1)
US9200073B2 (en) 2012-08-31 2015-12-01 Immunogen, Inc. Diagnostic assays and kits for detection of folate receptor 1
MX359950B (es) 2012-11-20 2018-10-17 Novartis Ag Casete de expresión optimizado para expresar un polipéptido con un alto rendimiento.
WO2014141037A1 (en) 2013-03-11 2014-09-18 Novartis Ag Method of screening cell clones
RU2716977C2 (ru) * 2013-07-31 2020-03-17 Новартис Аг Новые селективные векторы и способы селекции эукариотических клеток-хозяев
CN113150145B (zh) 2013-08-30 2025-02-14 伊缪诺金公司 用于检测叶酸受体1的抗体和测定
EP3083677B1 (en) 2013-12-20 2019-08-28 Novartis AG Novel eukaryotic cells and methods for recombinantly expressing a product of interest
JP6708548B2 (ja) 2013-12-20 2020-06-10 ノバルティス アーゲー 新規真核細胞および目的の生成物を組換え発現させるための方法
PL3137595T3 (pl) 2014-04-29 2019-09-30 Novartis Ag Nowe komórki kręgowców i sposoby ekspresji rekombinowanej polipeptydu będącego przedmiotem zainteresowania
US20180066268A1 (en) * 2015-04-03 2018-03-08 Selexis S.A. Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells
EP3328387B1 (en) * 2015-07-30 2020-07-22 Expression Pathology, Inc. Quantifying fr- and gart proteins for optimal cancer therapy
CN116440279B (zh) 2015-09-17 2026-02-24 伊缪诺金公司 包含抗folr1免疫缀合物的治疗组合
US20200370056A1 (en) 2017-10-02 2020-11-26 Astrazeneca Ab Cell lines and methods for increased protein production
CN116615462A (zh) 2020-11-23 2023-08-18 诺华股份有限公司 抗体构建体的表达技术
JP2024517711A (ja) 2021-04-29 2024-04-23 ノバルティス アーゲー 高シアル酸付加細胞
CN118985647A (zh) * 2024-08-13 2024-11-22 杭州卓智康悦科技有限公司 一种净化空气释放负离子的中药抑菌液及制备工艺

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0555203B1 (en) * 1988-12-08 1995-10-18 Abbott Laboratories Expression induction method employing dhfr gene
IL116872A (en) 1995-02-15 2004-12-15 Aventis Pharma Sa Delta-5, 7 sterol reductase from arabidopsis thaliana
US7824907B2 (en) * 2003-03-11 2010-11-02 Merck Serono Sa Expression vectors comprising the mCMV IE2 promoter
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
CN100567482C (zh) * 2006-10-12 2009-12-09 南开大学 高效表达rhBMP2的CHO细胞株及其建立方法

Also Published As

Publication number Publication date
BRPI0821401A2 (pt) 2014-10-14
CA2709503C (en) 2016-07-12
CA2709503A1 (en) 2009-07-02
JP5660900B2 (ja) 2015-01-28
CN101903526B (zh) 2014-02-12
RU2010129550A (ru) 2012-01-27
PT2227549E (pt) 2015-10-08
WO2009080759A1 (en) 2009-07-02
RU2502802C2 (ru) 2013-12-27
US20200370055A1 (en) 2020-11-26
KR101706390B1 (ko) 2017-02-13
EP2227549B1 (en) 2015-06-17
US20140295489A1 (en) 2014-10-02
CN101903526A (zh) 2010-12-01
KR20100111698A (ko) 2010-10-15
IL206233A0 (en) 2010-12-30
US10767186B2 (en) 2020-09-08
US20100330572A1 (en) 2010-12-30
US20160355828A1 (en) 2016-12-08
JP2011505861A (ja) 2011-03-03
JP2014236745A (ja) 2014-12-18
AU2008340017B2 (en) 2012-04-05
IL206233A (en) 2015-10-29
JP6002183B2 (ja) 2016-10-05
CN103805633A (zh) 2014-05-21
ES2546086T3 (es) 2015-09-18
EP2227549A1 (en) 2010-09-15
DK2227549T3 (en) 2015-09-21
AU2008340017A1 (en) 2009-07-02
CN103805633B (zh) 2016-09-07
US20120276579A1 (en) 2012-11-01

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