ES2546086T3 - Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana - Google Patents

Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana Download PDF

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Publication number
ES2546086T3
ES2546086T3 ES08865304.3T ES08865304T ES2546086T3 ES 2546086 T3 ES2546086 T3 ES 2546086T3 ES 08865304 T ES08865304 T ES 08865304T ES 2546086 T3 ES2546086 T3 ES 2546086T3
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Prior art keywords
membrane
cell culture
eukaryotic cell
selection system
receptor gene
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ES08865304.3T
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English (en)
Inventor
Yehuda G. Assaraf
Thomas Jostock
Hans-Peter Knopf
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Novartis AG
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un vector de expresión en eucariotas que comprende un primer polinucleótido que codifica un receptor funcional de folato unido a la membrana y un segundo polinucleótido que codifica un producto de interés, en donde el producto de interés es un polipéptido farmacéutica o terapéuticamente activo o de diagnóstico.

Description

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Claims (1)

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ES08865304.3T 2007-12-21 2008-12-19 Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana Active ES2546086T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP07150326 2007-12-21
EP07150326 2007-12-21
PCT/EP2008/068046 WO2009080759A1 (en) 2007-12-21 2008-12-19 Organic compounds

Publications (1)

Publication Number Publication Date
ES2546086T3 true ES2546086T3 (es) 2015-09-18

Family

ID=39560901

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08865304.3T Active ES2546086T3 (es) 2007-12-21 2008-12-19 Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana

Country Status (15)

Country Link
US (5) US20100330572A1 (es)
EP (1) EP2227549B1 (es)
JP (2) JP5660900B2 (es)
KR (1) KR101706390B1 (es)
CN (2) CN101903526B (es)
AU (1) AU2008340017B2 (es)
BR (1) BRPI0821401A2 (es)
CA (1) CA2709503C (es)
DK (1) DK2227549T3 (es)
ES (1) ES2546086T3 (es)
IL (1) IL206233A (es)
PL (1) PL2227549T3 (es)
PT (1) PT2227549E (es)
RU (1) RU2502802C2 (es)
WO (1) WO2009080759A1 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5801207B2 (ja) 2009-02-27 2015-10-28 ノバルティス アーゲー ２種類の選択マーカーを含む発現ベクター系
TWI622402B (zh) 2010-02-24 2018-05-01 免疫遺傳股份有限公司 葉酸受體１抗體類和免疫共軛物類及彼等之用途
BR112013025415B1 (pt) 2011-04-01 2021-03-16 Immunogen, Inc uso de um imunoconjugado antirreceptor de folato 1 (folr1)
US9200073B2 (en) 2012-08-31 2015-12-01 Immunogen, Inc. Diagnostic assays and kits for detection of folate receptor 1
MX359950B (es) 2012-11-20 2018-10-17 Novartis Ag Casete de expresión optimizado para expresar un polipéptido con un alto rendimiento.
WO2014141037A1 (en) 2013-03-11 2014-09-18 Novartis Ag Method of screening cell clones
RU2716977C2 (ru) * 2013-07-31 2020-03-17 Новартис Аг Новые селективные векторы и способы селекции эукариотических клеток-хозяев
CN113150145B (zh) 2013-08-30 2025-02-14 伊缪诺金公司 用于检测叶酸受体1的抗体和测定
EP3083677B1 (en) 2013-12-20 2019-08-28 Novartis AG Novel eukaryotic cells and methods for recombinantly expressing a product of interest
JP6708548B2 (ja) 2013-12-20 2020-06-10 ノバルティス アーゲー 新規真核細胞および目的の生成物を組換え発現させるための方法
PL3137595T3 (pl) 2014-04-29 2019-09-30 Novartis Ag Nowe komórki kręgowców i sposoby ekspresji rekombinowanej polipeptydu będącego przedmiotem zainteresowania
US20180066268A1 (en) * 2015-04-03 2018-03-08 Selexis S.A. Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells
EP3328387B1 (en) * 2015-07-30 2020-07-22 Expression Pathology, Inc. Quantifying fr- and gart proteins for optimal cancer therapy
CN116440279B (zh) 2015-09-17 2026-02-24 伊缪诺金公司 包含抗folr1免疫缀合物的治疗组合
US20200370056A1 (en) 2017-10-02 2020-11-26 Astrazeneca Ab Cell lines and methods for increased protein production
CN116615462A (zh) 2020-11-23 2023-08-18 诺华股份有限公司 抗体构建体的表达技术
JP2024517711A (ja) 2021-04-29 2024-04-23 ノバルティス アーゲー 高シアル酸付加細胞
CN118985647A (zh) * 2024-08-13 2024-11-22 杭州卓智康悦科技有限公司 一种净化空气释放负离子的中药抑菌液及制备工艺

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0555203B1 (en) * 1988-12-08 1995-10-18 Abbott Laboratories Expression induction method employing dhfr gene
IL116872A (en) 1995-02-15 2004-12-15 Aventis Pharma Sa Delta-5, 7 sterol reductase from arabidopsis thaliana
US7824907B2 (en) * 2003-03-11 2010-11-02 Merck Serono Sa Expression vectors comprising the mCMV IE2 promoter
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
CN100567482C (zh) * 2006-10-12 2009-12-09 南开大学 高效表达rhBMP2的CHO细胞株及其建立方法

Also Published As

Publication number Publication date
BRPI0821401A2 (pt) 2014-10-14
CA2709503C (en) 2016-07-12
CA2709503A1 (en) 2009-07-02
JP5660900B2 (ja) 2015-01-28
CN101903526B (zh) 2014-02-12
RU2010129550A (ru) 2012-01-27
PT2227549E (pt) 2015-10-08
WO2009080759A1 (en) 2009-07-02
RU2502802C2 (ru) 2013-12-27
US20200370055A1 (en) 2020-11-26
KR101706390B1 (ko) 2017-02-13
PL2227549T3 (pl) 2015-12-31
EP2227549B1 (en) 2015-06-17
US20140295489A1 (en) 2014-10-02
CN101903526A (zh) 2010-12-01
KR20100111698A (ko) 2010-10-15
IL206233A0 (en) 2010-12-30
US10767186B2 (en) 2020-09-08
US20100330572A1 (en) 2010-12-30
US20160355828A1 (en) 2016-12-08
JP2011505861A (ja) 2011-03-03
JP2014236745A (ja) 2014-12-18
AU2008340017B2 (en) 2012-04-05
IL206233A (en) 2015-10-29
JP6002183B2 (ja) 2016-10-05
CN103805633A (zh) 2014-05-21
EP2227549A1 (en) 2010-09-15
DK2227549T3 (en) 2015-09-21
AU2008340017A1 (en) 2009-07-02
CN103805633B (zh) 2016-09-07
US20120276579A1 (en) 2012-11-01

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