ES2546086T3 - Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana - Google Patents

Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana Download PDF

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Publication number
ES2546086T3
ES2546086T3 ES08865304.3T ES08865304T ES2546086T3 ES 2546086 T3 ES2546086 T3 ES 2546086T3 ES 08865304 T ES08865304 T ES 08865304T ES 2546086 T3 ES2546086 T3 ES 2546086T3
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Prior art keywords
membrane
cell culture
eukaryotic cell
selection system
receptor gene
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ES08865304.3T
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English (en)
Inventor
Yehuda G. Assaraf
Thomas Jostock
Hans-Peter Knopf
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Novartis AG
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un vector de expresión en eucariotas que comprende un primer polinucleótido que codifica un receptor funcional de folato unido a la membrana y un segundo polinucleótido que codifica un producto de interés, en donde el producto de interés es un polipéptido farmacéutica o terapéuticamente activo o de diagnóstico.

Description

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Claims (1)

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ES08865304.3T 2007-12-21 2008-12-19 Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana Active ES2546086T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP07150326 2007-12-21
EP07150326 2007-12-21
PCT/EP2008/068046 WO2009080759A1 (en) 2007-12-21 2008-12-19 Organic compounds

Publications (1)

Publication Number Publication Date
ES2546086T3 true ES2546086T3 (es) 2015-09-18

Family

ID=39560901

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08865304.3T Active ES2546086T3 (es) 2007-12-21 2008-12-19 Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana

Country Status (15)

Country Link
US (5) US20100330572A1 (es)
EP (1) EP2227549B1 (es)
JP (2) JP5660900B2 (es)
KR (1) KR101706390B1 (es)
CN (2) CN103805633B (es)
AU (1) AU2008340017B2 (es)
BR (1) BRPI0821401A2 (es)
CA (1) CA2709503C (es)
DK (1) DK2227549T3 (es)
ES (1) ES2546086T3 (es)
IL (1) IL206233A (es)
PL (1) PL2227549T3 (es)
PT (1) PT2227549E (es)
RU (1) RU2502802C2 (es)
WO (1) WO2009080759A1 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2753814C (en) 2009-02-27 2018-07-31 Novartis Ag Novel selection vectors and methods of selecting eukaryotic host cells
AR080301A1 (es) 2010-02-24 2012-03-28 Immunogen Inc Anticuerpos e inmunoconjugados del receptor 1 de folato y sus usos
PL2694106T3 (pl) 2011-04-01 2018-06-29 Immunogen, Inc. Sposoby zwiększania skuteczności terapii przeciwnowotworowej opartej na receptorze folr1
KR20150046316A (ko) 2012-08-31 2015-04-29 이뮤노젠 아이엔씨 엽산 수용체 1의 검출을 위한 진단성 검정 및 키트
KR102286389B1 (ko) 2012-11-20 2021-08-05 노파르티스 아게 폴리펩티드를 고 수율로 발현하는 최적화 발현 카세트
EP2970956A1 (en) 2013-03-11 2016-01-20 Novartis AG Method of screening cell clones
EP3027646B1 (en) * 2013-07-31 2018-06-27 Novartis AG Novel selection vectors and methods of selecting eukaryotic host cells
PT3038650T (pt) 2013-08-30 2021-08-25 Immunogen Inc Anticorpos e ensaios para a deteção do recetor de folato 1
CA2934411C (en) 2013-12-20 2022-05-03 Novartis Ag Novel eukaryotic cells and methods for recombinantly expressing a product of interest
SG11201604218RA (en) 2013-12-20 2016-07-28 Novartis Ag Novel eukaryotic cells and methods for recombinantly expressing a product of interest
EP4332231A3 (en) 2014-04-29 2024-05-29 Novartis AG Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest
KR102622657B1 (ko) * 2015-04-03 2024-01-10 셀렉시스 에스. 에이. 포유류 세포에서 재조합 단백질 생산을 위한 비타민 및 비타민 대사 유전자 및 단백질의 용도
WO2017020048A1 (en) * 2015-07-30 2017-02-02 Expression Pathology, Inc. QUANTIFYING FR-α AND GART PROTEINS FOR OPTIMAL CANCER THERAPY
JP6880006B2 (ja) 2015-09-17 2021-06-02 イミュノジェン， インコーポレイテッド 抗ｆｏｌｒ１免疫複合体を含む治療組み合わせ
WO2019070638A1 (en) 2017-10-02 2019-04-11 Astrazeneca Ab CELL LINES AND METHODS FOR INCREASED PROTEIN PRODUCTION
US20250282894A1 (en) 2020-11-23 2025-09-11 Novartis Ag Expression technology for antibody constructs
EP4330383A1 (en) 2021-04-29 2024-03-06 Novartis AG Hypersialylating cells
CN118985647A (zh) * 2024-08-13 2024-11-22 杭州卓智康悦科技有限公司 一种净化空气释放负离子的中药抑菌液及制备工艺

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU644352B2 (en) * 1988-12-08 1993-12-09 Damon Biotech Inc. Expression induction method employing mutant dhfr gene
IL162476A (en) * 1995-02-15 2005-12-18 Aventis Pharma Sa Arabidopsis thaliana proteins having delta -5,7- sterol - delta -7- reductase activity
ATE399872T1 (de) * 2003-03-11 2008-07-15 Serono Lab Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
CN100567482C (zh) * 2006-10-12 2009-12-09 南开大学 高效表达rhBMP2的CHO细胞株及其建立方法

Also Published As

Publication number Publication date
AU2008340017B2 (en) 2012-04-05
BRPI0821401A2 (pt) 2014-10-14
EP2227549A1 (en) 2010-09-15
US10767186B2 (en) 2020-09-08
CA2709503C (en) 2016-07-12
KR101706390B1 (ko) 2017-02-13
IL206233A (en) 2015-10-29
JP2011505861A (ja) 2011-03-03
CN101903526B (zh) 2014-02-12
US20100330572A1 (en) 2010-12-30
AU2008340017A1 (en) 2009-07-02
JP6002183B2 (ja) 2016-10-05
JP5660900B2 (ja) 2015-01-28
PT2227549E (pt) 2015-10-08
KR20100111698A (ko) 2010-10-15
DK2227549T3 (en) 2015-09-21
CN103805633B (zh) 2016-09-07
EP2227549B1 (en) 2015-06-17
PL2227549T3 (pl) 2015-12-31
RU2502802C2 (ru) 2013-12-27
US20120276579A1 (en) 2012-11-01
WO2009080759A1 (en) 2009-07-02
US20200370055A1 (en) 2020-11-26
JP2014236745A (ja) 2014-12-18
US20140295489A1 (en) 2014-10-02
IL206233A0 (en) 2010-12-30
RU2010129550A (ru) 2012-01-27
CN103805633A (zh) 2014-05-21
CA2709503A1 (en) 2009-07-02
CN101903526A (zh) 2010-12-01
US20160355828A1 (en) 2016-12-08

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