PL2691410T3 - Oczyszczanie trifosforylowanych oligonukleotydów z użyciem znaczników wychwytu - Google Patents

Oczyszczanie trifosforylowanych oligonukleotydów z użyciem znaczników wychwytu

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Publication number
PL2691410T3
PL2691410T3 PL12710950T PL12710950T PL2691410T3 PL 2691410 T3 PL2691410 T3 PL 2691410T3 PL 12710950 T PL12710950 T PL 12710950T PL 12710950 T PL12710950 T PL 12710950T PL 2691410 T3 PL2691410 T3 PL 2691410T3
Authority
PL
Poland
Prior art keywords
purification
capture tags
triphosphorylated
oligonucleotides
triphosphorylated oligonucleotides
Prior art date
Application number
PL12710950T
Other languages
English (en)
Inventor
Janos Ludwig
Marion Goldeck
Brian Sproat
Original Assignee
Rheinische Friedrich-Wilhelms-Universität Bonn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rheinische Friedrich-Wilhelms-Universität Bonn filed Critical Rheinische Friedrich-Wilhelms-Universität Bonn
Publication of PL2691410T3 publication Critical patent/PL2691410T3/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C07H1/04—Introducing polyphosphoric acid radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
PL12710950T 2011-03-28 2012-03-28 Oczyszczanie trifosforylowanych oligonukleotydów z użyciem znaczników wychwytu PL2691410T3 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11160032A EP2508530A1 (en) 2011-03-28 2011-03-28 Purification of triphosphorylated oligonucleotides using capture tags
EP12710950.2A EP2691410B1 (en) 2011-03-28 2012-03-28 Purification of triphosphorylated oligonucleotides using capture tags

Publications (1)

Publication Number Publication Date
PL2691410T3 true PL2691410T3 (pl) 2017-07-31

Family

ID=44453820

Family Applications (1)

Application Number Title Priority Date Filing Date
PL12710950T PL2691410T3 (pl) 2011-03-28 2012-03-28 Oczyszczanie trifosforylowanych oligonukleotydów z użyciem znaczników wychwytu

Country Status (18)

Country Link
US (2) US9399658B2 (pl)
EP (3) EP2508530A1 (pl)
JP (2) JP5981985B2 (pl)
CN (2) CN103492405B (pl)
AU (2) AU2012234296B2 (pl)
CA (1) CA2830980C (pl)
CY (1) CY1118867T1 (pl)
DK (1) DK2691410T3 (pl)
ES (1) ES2623002T3 (pl)
HR (1) HRP20170577T1 (pl)
HU (1) HUE033843T2 (pl)
LT (1) LT2691410T (pl)
PL (1) PL2691410T3 (pl)
PT (1) PT2691410T (pl)
RS (1) RS55912B1 (pl)
SI (1) SI2691410T1 (pl)
SM (1) SMT201700211T1 (pl)
WO (1) WO2012130886A1 (pl)

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SI2056845T1 (en) 2006-08-08 2018-02-28 Rheinische Friedrich-Wilhelms-Universitaet Bonn STRUCTURE AND USE 5 'PHOSPHATE OF OLIGONUCLEOTES
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EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
EP2712870A1 (en) * 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
US10947543B2 (en) 2013-03-13 2021-03-16 Yale University Interferon production using short RNA duplexes
DE102015008536A1 (de) 2015-07-02 2017-01-05 Rheinische Friedrich-Wilhelms-Universität Bonn Diskontinuierliche Oligonukleotid-Liganden
US10793901B2 (en) * 2016-12-28 2020-10-06 Roche Molecular Systems, Inc. Reversibly protected nucleotide reagents with high thermal stability
US11382966B2 (en) 2017-03-24 2022-07-12 Rigontec Gmbh Method for designing RIG-I ligands
KR102724871B1 (ko) * 2017-08-18 2024-10-31 애질런트 테크놀로지스, 인크. 올리고뉴클레오티드의 친화성 정제를 위한 오르토에스터 조성물
JP7366057B2 (ja) 2018-04-19 2023-10-20 チェックメイト ファーマシューティカルズ， インコーポレイテッド 合成ｒｉｇ－ｉ様受容体アゴニスト
WO2019246450A1 (en) 2018-06-20 2019-12-26 Yale University Rig-i agonists and treatments using same
EP3990635A1 (en) 2019-06-27 2022-05-04 Rigontec GmbH Design method for optimized rig-i ligands
US11786545B2 (en) 2020-12-09 2023-10-17 Yale University Compositions and methods for treating SARS-CoV-2 infection
US11649457B2 (en) 2020-12-09 2023-05-16 Yale University Methods for treating SARS-CoV-2 infection

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