PL364051A1 - Sposoby oczyszczania DNA i oczyszczony DNA - Google Patents

Sposoby oczyszczania DNA i oczyszczony DNA

Info

Publication number
PL364051A1
PL364051A1 PL00364051A PL36405100A PL364051A1 PL 364051 A1 PL364051 A1 PL 364051A1 PL 00364051 A PL00364051 A PL 00364051A PL 36405100 A PL36405100 A PL 36405100A PL 364051 A1 PL364051 A1 PL 364051A1
Authority
PL
Poland
Prior art keywords
dna
purification
methods
plasmid dna
purified
Prior art date
Application number
PL00364051A
Other languages
English (en)
Other versions
PL203013B1 (pl
Inventor
Natarajan Ramasubramanyan
Original Assignee
Cambrex Bio Science Baltimore Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22474295&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL364051(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cambrex Bio Science Baltimore Inc filed Critical Cambrex Bio Science Baltimore Inc
Publication of PL364051A1 publication Critical patent/PL364051A1/pl
Publication of PL203013B1 publication Critical patent/PL203013B1/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PL364051A 1999-05-28 2000-05-26 Sposób oczyszczania plazmidowego DNA, sposób oddzielania superskręconego plazmidowego DNA, sposób oddzielania endotoksyny od plazmidowego DNA, sposób wzbogacania ilości superskręconego DNA względem rozluźnionego DNA, sposób usuwania lipopolisacharydu PL203013B1 (pl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13677299P 1999-05-28 1999-05-28

Publications (2)

Publication Number Publication Date
PL364051A1 true PL364051A1 (pl) 2004-12-13
PL203013B1 PL203013B1 (pl) 2009-08-31

Family

ID=22474295

Family Applications (1)

Application Number Title Priority Date Filing Date
PL364051A PL203013B1 (pl) 1999-05-28 2000-05-26 Sposób oczyszczania plazmidowego DNA, sposób oddzielania superskręconego plazmidowego DNA, sposób oddzielania endotoksyny od plazmidowego DNA, sposób wzbogacania ilości superskręconego DNA względem rozluźnionego DNA, sposób usuwania lipopolisacharydu

Country Status (21)

Country Link
EP (1) EP1187840B2 (pl)
JP (2) JP5320528B2 (pl)
KR (1) KR100688349B1 (pl)
CN (1) CN1264854C (pl)
AT (1) ATE433456T1 (pl)
AU (1) AU782153B2 (pl)
BR (1) BR0011033A (pl)
CA (1) CA2375215C (pl)
CZ (1) CZ20014274A3 (pl)
DE (1) DE60042366D1 (pl)
DK (1) DK1187840T4 (pl)
ES (1) ES2328008T5 (pl)
HU (1) HUP0301869A2 (pl)
IL (3) IL146695A0 (pl)
MX (1) MXPA01012213A (pl)
NO (1) NO20015703L (pl)
NZ (1) NZ515645A (pl)
PL (1) PL203013B1 (pl)
RO (1) RO122855B1 (pl)
RU (1) RU2001135838A (pl)
WO (1) WO2000073318A1 (pl)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2001135838A (ru) * 1999-05-28 2004-01-20 Камбрекс Байо Сайенс Инк. (Us) Способы очистки ДНК и очищенная ДНК
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내dna 또는 rna로부터 리포폴리사카라이드를제거하는 방법
SE0200543D0 (sv) * 2002-02-21 2002-02-21 Amersham Biosciences Ab Method of separation using aromatic thioether ligands
US20040127648A1 (en) * 2002-12-31 2004-07-01 Ciphergen Biosystems, Inc. Sorbent and method for the separation of plasmid DNA
CA2536931A1 (en) * 2003-09-17 2005-03-24 Centelion Method of preparation of pharmaceutically grade plasmid dna
NZ549835A (en) * 2004-04-19 2008-12-24 Centelion Method for purifying plasmid DNA
KR100612869B1 (ko) * 2004-11-08 2006-08-14 삼성전자주식회사 고정화된 금속-리간드 복합체에 의한 세포 용해 방법
JP4811773B2 (ja) * 2005-06-08 2011-11-09 肇 柄谷 核酸の精製法
WO2007114762A1 (en) * 2006-03-31 2007-10-11 Ge Healthcare Bio-Sciences Ab Method of separation of deoxyribonucleic acids
US20120220729A1 (en) 2011-02-28 2012-08-30 Boehringer Ingelheim International Gmbh Liquid phase separation of plasmid dna isoforms and topoisomers
JPWO2015050191A1 (ja) 2013-10-03 2017-03-09 協和発酵バイオ株式会社 二重鎖リボ核酸の精製方法
BR112018075667B1 (pt) * 2016-06-14 2024-02-20 Biogen Ma, Inc Métodos para separar um oligonucleotídeo alvo de uma mistura contendo o oligonucleotídeo alvo e uma impureza relacionada com o produto
US20210002667A1 (en) * 2018-03-15 2021-01-07 Intergalactic Therapeutics, Inc. Synthetic dna vectors and methods of use
US11865480B2 (en) 2019-05-07 2024-01-09 Electronics and Telecommunications Research Institute Cancer Rop Co., Ltd. Nucleic acid purification device and nucleic acid purification method
KR20220128607A (ko) 2019-09-18 2022-09-21 인터갈락틱 테라퓨틱스, 인크. 합성 dna 벡터 및 사용 방법
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
KR20220018187A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출키트
KR20220018185A (ko) 2020-08-06 2022-02-15 주식회사 바이오솔루션 플라스미드 dna 추출방법
WO2022073995A1 (en) * 2020-10-05 2022-04-14 Bia Separations D.O.O. Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622960A (en) * 1992-04-14 1997-04-22 The United States Of America As Represented By The Department Of Health And Human Services Topoisomerase II inhibitors and therapeutic uses therefor
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
JP4113580B2 (ja) 1994-02-07 2008-07-09 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング エンドトキシンを減量又は除去する方法
US5707850A (en) 1995-11-09 1998-01-13 Cole; Kenneth D. Concentration and size-fractionation of nucleic acids and viruses in porous media
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6441160B2 (en) * 1998-05-11 2002-08-27 Tosoh Corporation Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography
JP2000035423A (ja) * 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
RU2001135838A (ru) * 1999-05-28 2004-01-20 Камбрекс Байо Сайенс Инк. (Us) Способы очистки ДНК и очищенная ДНК

Also Published As

Publication number Publication date
CA2375215C (en) 2012-11-20
CN1390229A (zh) 2003-01-08
JP2012050463A (ja) 2012-03-15
MXPA01012213A (es) 2003-06-24
EP1187840B1 (en) 2009-06-10
AU782153B2 (en) 2005-07-07
ES2328008T3 (es) 2009-11-06
KR20020092778A (ko) 2002-12-12
PL203013B1 (pl) 2009-08-31
IL146695A0 (en) 2002-07-25
EP1187840A1 (en) 2002-03-20
IL222755A (en) 2015-08-31
IL222755A0 (en) 2012-12-31
IL146695A (en) 2012-12-31
RO122855B1 (ro) 2010-03-30
KR100688349B1 (ko) 2007-02-28
ES2328008T5 (es) 2013-02-25
DK1187840T4 (da) 2013-02-04
DK1187840T3 (da) 2009-10-19
NO20015703L (no) 2002-01-03
JP5320528B2 (ja) 2013-10-23
JP2003524405A (ja) 2003-08-19
DE60042366D1 (de) 2009-07-23
CN1264854C (zh) 2006-07-19
BR0011033A (pt) 2003-07-01
AU5165500A (en) 2000-12-18
NZ515645A (en) 2004-07-30
RU2001135838A (ru) 2004-01-20
ATE433456T1 (de) 2009-06-15
EP1187840B2 (en) 2012-12-19
CZ20014274A3 (cs) 2003-01-15
WO2000073318A1 (en) 2000-12-07
HUP0301869A2 (hu) 2003-08-28
NO20015703D0 (no) 2001-11-22
CA2375215A1 (en) 2000-12-07
EP1187840A4 (en) 2002-09-18

Similar Documents

Publication Publication Date Title
IL222755A0 (en) Methods of dna purification and purified dna
DK0923592T3 (da) Fremgangsmåder til rensning af nukleinsyrer
GB2363795A (en) Process for nucleic acid purification using silicon carbide
CA2182388A1 (en) Process for producing endotoxin-free or endotoxin-poor nucleic acids and/or oligonucleotides for gene therapy
WO1996019568A3 (en) Stabilized ribozyme analogs
AU2001264811A1 (en) Methods for genetic analysis of dna to detect sequence variances
DK0973928T3 (da) DNA-baseret transposonsystem til indføring af nukleinsyre i DNA i en celle
AU6548096A (en) Solution containing chaotropic agent and process using it for isolation of dna, rna and proteins
AU2434195A (en) Thymidine kinase mutants
AU7225591A (en) Nucleic acid enzymes for cleaving dna
AU5720498A (en) Chemical synthesis of nucleosides analogs and their incorporation into polynucleotides
IL145411A0 (en) Cleavage of nucleic acid from solid supports
AU2001275688A1 (en) Multiplex sequencing method
AU2003293042A1 (en) Process for purification of plasmid dna
WO2002044414A3 (en) Purification of dna sequencing reactions using silica magnetic particles
DK1181089T3 (da) Fremgangsmåde til separering af organiske stoffer fra en vandig blanding
DE60042343D1 (de) Abtrennung von fibrinogen von plasmaproteasen
AU6329099A (en) Method for purifying or isolating viral nucleic acids
CA2316113A1 (en) Method for nucleic acid purification using iodine
WO2001039720A3 (fr) Inhibiteurs des adn polymerases, le mikanolide et le dihydromikanolide
AU3944385A (en) Process for the dissolution of nucleotides and polynucleotides in non-aqueous solvents and mixed non-aqueous/aqueous solvent systems, solutions thereby obtained and their use
DE69904315D1 (de) System zur trennung von verunreinigungen von strahlmitteln
AU2003225983A1 (en) Cpg retrieval of dna from formalin-fixed pathology specimen for promoter methylation analysis
AU2001272933A1 (en) Analysis of data from liquid chromatographic separation of dna
AU2002309610A1 (en) Synthetic nucleic acid molecule for imparting multiple traits