PL415317A1 - Method for producing polylactide sponge-like scaffolds for the culture of cylindrical epithelium - Google Patents
Method for producing polylactide sponge-like scaffolds for the culture of cylindrical epitheliumInfo
- Publication number
- PL415317A1 PL415317A1 PL415317A PL41531715A PL415317A1 PL 415317 A1 PL415317 A1 PL 415317A1 PL 415317 A PL415317 A PL 415317A PL 41531715 A PL41531715 A PL 41531715A PL 415317 A1 PL415317 A1 PL 415317A1
- Authority
- PL
- Poland
- Prior art keywords
- solution
- water
- polylactide
- polymer
- solvent
- Prior art date
Links
- 229920000747 poly(lactic acid) Polymers 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 210000000981 epithelium Anatomy 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 5
- 229920000642 polymer Polymers 0.000 abstract 4
- 239000002904 solvent Substances 0.000 abstract 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 abstract 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 abstract 1
- 210000002919 epithelial cell Anatomy 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
Landscapes
- Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Materials For Medical Uses (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób wytwarzania polilaktydowych rusztowań gąbczastych do hodowli komórek nabłonka walcowatego, zgodnie z którym w reaktorze szklanym umieszcza się roztwór polilaktydu w dioksanie, dichlorometanie lub chloroformie o stężeniu 1,5 - 6% wag., roztwór miesza się przez 20 - 28 h z szybkością 550 - 700 obr/min., w temperaturze 20 - 30°C, do całkowitego rozpuszczenia polimeru, następnie do roztworu dodaje się nierozpuszczalnik polilaktydu, którym jest woda w takiej ilości, aby stężenie nierozpuszczalnika wynosiło od 0 - 5% wag. w stosunku do masy polimeru, po czym, po uzyskaniu jednorodnego roztworu polimeru, roztwór zamraża się przez 20 - 28 h w temperaturze -10 do -20°C i uzyskane zamrożone rusztowanie poddaje się żelowaniu w wodzie z metanolem i/albo etanolem w stosunku objętościowym wody do alkoholu 0,01 - 0,1, przy czym objętość kąpieli żelującej jest minimum 30 razy większa niż objętość roztworu polimeru w rozpuszczalniku, zaś etap żelowania prowadzi się w temperaturze od -10°C do -20°C przez 30 - 48 h, a następnie otrzymane rusztowanie oczyszcza się w wodzie w ciągu 0,5 - 1,5 h, po czym suszy się z pozostałości wody i rozpuszczalników przez 60 - 80 h pod zmniejszonym ciśnieniem, w temperaturze poniżej 50°C.The subject of the application is a method for producing spongy polylactide scaffolds for the cultivation of cylindrical epithelial cells, according to which a solution of polylactide in dioxane, dichloromethane or chloroform at a concentration of 1.5 - 6% by weight is placed in a glass reactor, the solution is mixed for 20-28 h at a speed of 550 - 700 rpm, at a temperature of 20-30 ° C, until the polymer is completely dissolved, then a polylactide non-solvent is added to the solution, which is water in such an amount that the concentration of the non-solvent is from 0-5 wt. based on the weight of the polymer, after which, after obtaining a homogeneous polymer solution, the solution is frozen for 20 - 28 h at -10 to -20 ° C and the obtained frozen scaffold is gelatinized in water with methanol and / or ethanol in a volume ratio of water to alcohol 0.01 - 0.1, the volume of the gelling bath is a minimum 30 times greater than the volume of the polymer solution in the solvent, while the gelling stage is carried out at a temperature from -10 ° C to -20 ° C for 30 - 48 h, and then the obtained scaffold is cleaned in water within 0.5 - 1.5 h, after which it is dried from residual water and solvents for 60 - 80 h under reduced pressure, at a temperature below 50 ° C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL415317A PL230136B1 (en) | 2015-12-15 | 2015-12-15 | Method for producing polylactide sponge-like scaffolds for the culture of cylindrical epithelium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL415317A PL230136B1 (en) | 2015-12-15 | 2015-12-15 | Method for producing polylactide sponge-like scaffolds for the culture of cylindrical epithelium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL415317A1 true PL415317A1 (en) | 2017-06-19 |
| PL230136B1 PL230136B1 (en) | 2018-09-28 |
Family
ID=59061579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL415317A PL230136B1 (en) | 2015-12-15 | 2015-12-15 | Method for producing polylactide sponge-like scaffolds for the culture of cylindrical epithelium |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL230136B1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL442689A1 (en) * | 2022-10-30 | 2024-05-06 | Uniwersytet Rzeszowski | Three-component bioactive composite for non-contact heat generation under the influence of an alternating magnetic field and infrared, its production method and its application |
-
2015
- 2015-12-15 PL PL415317A patent/PL230136B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL230136B1 (en) | 2018-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2013104993A (en) | METHOD FOR PRODUCING CELLULOSE FILM | |
| Siddeek et al. | MicroRNAs as potential biomarkers in diseases and toxicology | |
| WO2012134878A3 (en) | Polyurethane composites produced by a vacuum infusion process | |
| JP2015061520A5 (en) | ||
| FR3029525B1 (en) | POLY (ITACONATE) RETICLE BIOSOURCE, INCLUDING COMPOSITIONS AND USES AS SUPER ABSORBENT POLYMER | |
| PL433698A1 (en) | Method of producing a highly flexible cell scaffold | |
| CN108026326B (en) | Solvent composition comprising a mixture of molecules having sulfoxide functional groups and molecules having amide functional groups | |
| JP6725648B2 (en) | Polymer gel, method for producing the same, and use thereof | |
| CN105038353A (en) | A low-VOC, zero-degree film-forming latex paint for anti-formaldehyde interior walls | |
| GEP20257758B (en) | Process for direct sulfation of polysaccharides in an ecologically acceptable solvent | |
| NZ730713A (en) | Dissolution testing of hydrophobic active pharmaceutical ingredients such as avermectins with or without pyrantel | |
| US2317767A (en) | Cellulose fatty acid esters | |
| PL403697A1 (en) | Method of preparation of the chitosan solution, composition and method of making the chitosan hydrogel membrane | |
| CN105017553A (en) | Foaming agent for thermal insulation polyurethane plastic | |
| BR112015010917A2 (en) | process for the production of rigid foamed objects made of polymeric materials | |
| Lee et al. | Study on novel porous nasal scaffold using injection molding and particle leaching | |
| PL426819A1 (en) | Method of producing polylactide spongy bone substitute with increased hydrophilicity | |
| Jingxue et al. | Uncertainty Assessment of Temperature and Precipitation Reanalysis Data in Heihe River Basin# br | |
| PL230136B1 (en) | Method for producing polylactide sponge-like scaffolds for the culture of cylindrical epithelium | |
| BR112017007793A2 (en) | batch blending process for ethylcellulose polymer dispersions | |
| Andersson | Structure control by phase separation and influence on mass transport in films for controlled release | |
| CN103694474B (en) | Netted polyimide foam preparation method | |
| TH173476A (en) | The production process of stable viscosity natural rubber by the carbonyl removal method. | |
| Szabo | REDEFINITION OF THE HOUSE-ARCHETYPE. ONE OF THE TYPICAL ARCHITECTURAL STRATEGIES AFTER THE PERIOD OF REGIONALISM IN HUNGARY TODAY | |
| PL427963A1 (en) | Dressing layer built on the basis of chitin ester derivatives with the addition of chondroitin sulfate |