PL423116A1 - Sposób uzyskiwania rekombinantowej, biologicznie aktywnej butyrylocholinoesterazy końskiej i jej pochodnych oraz sposób uzyskiwania biologicznie aktywnych cholinoesteraz oraz ich pochodnych w mikroorganizmie Leishmania tarentolae - Google Patents

Sposób uzyskiwania rekombinantowej, biologicznie aktywnej butyrylocholinoesterazy końskiej i jej pochodnych oraz sposób uzyskiwania biologicznie aktywnych cholinoesteraz oraz ich pochodnych w mikroorganizmie Leishmania tarentolae

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Publication number
PL423116A1
PL423116A1 PL423116A PL42311617A PL423116A1 PL 423116 A1 PL423116 A1 PL 423116A1 PL 423116 A PL423116 A PL 423116A PL 42311617 A PL42311617 A PL 42311617A PL 423116 A1 PL423116 A1 PL 423116A1
Authority
PL
Poland
Prior art keywords
biologically active
derivatives
obtaining
microorganism
leishmania tarentolae
Prior art date
Application number
PL423116A
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English (en)
Other versions
PL240561B1 (pl
Inventor
Piotr Skowron
Jacek Jasiecki
Original Assignee
Gdanski Univ Medyczny
Genozym Spolka Z Ograniczona Odpowiedzialnoscia
Univ Gdanski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Gdanski Univ Medyczny, Genozym Spolka Z Ograniczona Odpowiedzialnoscia, Univ Gdanski filed Critical Gdanski Univ Medyczny
Priority to PL423116A priority Critical patent/PL240561B1/pl
Priority to EP18199472.4A priority patent/EP3473709B1/en
Publication of PL423116A1 publication Critical patent/PL423116A1/pl
Publication of PL240561B1 publication Critical patent/PL240561B1/pl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01007—Acetylcholinesterase (3.1.1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01008—Cholinesterase (3.1.1.8), i.e. butyrylcholine-esterase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Przedmiotem zgłoszenia jest wektor DNA, zdolny do transformacji i integracji z chromosomem Leishmania tarentolae, zawierający gen kodujący skróconą wersję BChE końskiej, pozbawioną domeny tetrameryzacyjnej, kierujący biosyntezą Otwartej Ramy Odczytu. Zgłoszenie obejmuje też sposób uzyskiwania rekombinantowych, biologicznie aktywnych białek cholinoesteraz (skrót: "ChE"), konstrukcji klonów, ekspresji genetycznej w mikroorganizmie - protozoanie Leishmania tarentolae, wektorów eksprymujących sekwencje DNA kodujące ChE i sposób ich izolowania w formie biologicznie czynnego rekombinanatowego białka na przykładzie enzymu butyrylocholinoesterazy końskiej (E.C. 3.1.1.8; skrót: "BChE") i jego pochodnych.
PL423116A 2017-10-09 2017-10-09 Wektory DNA zdolne do transformacji i integracji z chromosomem Leishmania tarentolae zawierające gen kodujący butyrylocholinoesterazę BChE PL240561B1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL423116A PL240561B1 (pl) 2017-10-09 2017-10-09 Wektory DNA zdolne do transformacji i integracji z chromosomem Leishmania tarentolae zawierające gen kodujący butyrylocholinoesterazę BChE
EP18199472.4A EP3473709B1 (en) 2017-10-09 2018-10-09 Dna vector containing a gene encoding an equine butyrylcholinesterase, for transformation and integration into leishmania tarentolae chromosome.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL423116A PL240561B1 (pl) 2017-10-09 2017-10-09 Wektory DNA zdolne do transformacji i integracji z chromosomem Leishmania tarentolae zawierające gen kodujący butyrylocholinoesterazę BChE

Publications (2)

Publication Number Publication Date
PL423116A1 true PL423116A1 (pl) 2019-04-23
PL240561B1 PL240561B1 (pl) 2022-05-02

Family

ID=64308453

Family Applications (1)

Application Number Title Priority Date Filing Date
PL423116A PL240561B1 (pl) 2017-10-09 2017-10-09 Wektory DNA zdolne do transformacji i integracji z chromosomem Leishmania tarentolae zawierające gen kodujący butyrylocholinoesterazę BChE

Country Status (2)

Country Link
EP (1) EP3473709B1 (pl)
PL (1) PL240561B1 (pl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117305432B (zh) * 2023-07-20 2024-09-06 中国农业科学院北京畜牧兽医研究所 与中国家马丁酰胆碱酯酶活性相关的snp标记及其检测引物与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL89703A (en) 1989-03-21 2001-10-31 Yissum Res Dev Co Polynucleotide encoding human acetylcholinesterase, vectors comprising said polynucleotide, cells transformed by said vectors, enzyme produced by said transformed cell, and uses thereof
US5932780A (en) 1994-02-28 1999-08-03 Yissum Research Development Company Of Hebrew University Of Jerusalem Transgenic non-human animal assay system for anti-cholinesterase substances
AU1997901A (en) * 1999-11-05 2001-05-14 Jena Bioscience Gmbh Protein expression systems for non-pathogenic kinetoplastidae

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ALICIA J. DAFFERNER, CHARACTERIZATION OF BUTYRYLCHOLINESTERASE IN BOVINE SERUM, 8 February 2017 (2017-02-08) *
D.G. ILYUSHIN, RECOMBINANT HUMAN BUTYRYLCHOLINESTERASE AS A NEW-AGE BIOSCAVENGER DRUG: DEVELOPMENT OF THE EXPRESSION SYSTEM, 2013 *
RENEE M. BLONG, TETRAMERIZATION DOMAIN OF HUMAN BUTYRYLCHOLINESTERASE IS AT THE C-TERMINUS, 1997 *

Also Published As

Publication number Publication date
EP3473709A1 (en) 2019-04-24
PL240561B1 (pl) 2022-05-02
EP3473709B1 (en) 2021-12-08

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