PL429258A1 - Aptamer DNA wiążący metkę lizylową i jego zastosowanie - Google Patents

Aptamer DNA wiążący metkę lizylową i jego zastosowanie

Info

Publication number
PL429258A1
PL429258A1 PL429258A PL42925819A PL429258A1 PL 429258 A1 PL429258 A1 PL 429258A1 PL 429258 A PL429258 A PL 429258A PL 42925819 A PL42925819 A PL 42925819A PL 429258 A1 PL429258 A1 PL 429258A1
Authority
PL
Poland
Prior art keywords
tag
dna aptamer
lysyl
molecule
aptamer binding
Prior art date
Application number
PL429258A
Other languages
English (en)
Other versions
PL237531B1 (pl
Inventor
Klaudia Arciszewska
Filip BARTNICKI
Ewa Kowalska
Wojciech STRZAŁKA
Original Assignee
Uniwersytet Jagielloński
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL429258A priority Critical patent/PL237531B1/pl
Publication of PL429258A1 publication Critical patent/PL429258A1/pl
Publication of PL237531B1 publication Critical patent/PL237531B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Przedmiotem zgłoszenia jest aptamer DNA wykazujący powinowactwo do metki Lys i posiadający sekwencję nukleotydową przedstawioną jako Sekw. Nr Id. 1. Zgłoszenie obejmuje również zastosowanie ww oligonukleotydu do wytwarzania cząsteczki posiadającej powinowactwo do metki Lys, w szczególności: do detekcji cząsteczki zawierającej metkę Lys, do wiązania cząsteczki zawierającej metkę Lys, do oczyszczania cząsteczki zawierającej metkę Lys.
PL429258A 2019-03-14 2019-03-14 Aptamer DNA wiążący metkę lizylową i jego zastosowanie PL237531B1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL429258A PL237531B1 (pl) 2019-03-14 2019-03-14 Aptamer DNA wiążący metkę lizylową i jego zastosowanie

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL429258A PL237531B1 (pl) 2019-03-14 2019-03-14 Aptamer DNA wiążący metkę lizylową i jego zastosowanie

Publications (2)

Publication Number Publication Date
PL429258A1 true PL429258A1 (pl) 2020-09-21
PL237531B1 PL237531B1 (pl) 2021-04-19

Family

ID=72561429

Family Applications (1)

Application Number Title Priority Date Filing Date
PL429258A PL237531B1 (pl) 2019-03-14 2019-03-14 Aptamer DNA wiążący metkę lizylową i jego zastosowanie

Country Status (1)

Country Link
PL (1) PL237531B1 (pl)

Also Published As

Publication number Publication date
PL237531B1 (pl) 2021-04-19

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