PL431985A1 - Oligonucleotide primers and molecular probe for detecting fitopathogenic botrytis sp. fungus and method for its detection - Google Patents

Oligonucleotide primers and molecular probe for detecting fitopathogenic botrytis sp. fungus and method for its detection

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Publication number
PL431985A1
PL431985A1 PL431985A PL43198519A PL431985A1 PL 431985 A1 PL431985 A1 PL 431985A1 PL 431985 A PL431985 A PL 431985A PL 43198519 A PL43198519 A PL 43198519A PL 431985 A1 PL431985 A1 PL 431985A1
Authority
PL
Poland
Prior art keywords
molecular probe
detecting
detection
botrytis
oligonucleotide primers
Prior art date
Application number
PL431985A
Other languages
Polish (pl)
Other versions
PL239135B1 (en
Inventor
Jacek Panek
Magdalena Frąc
Dominika Malarczyk
Original Assignee
Instytut Agrofizyki Im.Bohdana Dobrzańskiego Polskiej Akademii Nauk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Agrofizyki Im.Bohdana Dobrzańskiego Polskiej Akademii Nauk filed Critical Instytut Agrofizyki Im.Bohdana Dobrzańskiego Polskiej Akademii Nauk
Priority to PL431985A priority Critical patent/PL239135B1/en
Publication of PL431985A1 publication Critical patent/PL431985A1/en
Publication of PL239135B1 publication Critical patent/PL239135B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiot wynalazku stanowią startery oligonukleotydowe oraz sonda molekularna do wykrywania patogenów grzybowych należących do rodzaju Botrytis o sekwencjach nr 1, 2 i 3, przedstawionych na liście sekwencji. Przedmiotem wynalazku jest również sposób wykrywania fitopatogenicznego grzyba z rodzaju Botrytis, w którym w reakcji qPCR stosuje się wymienioną powyżej parę starterów i sondę molekularną.The subject of the invention are oligonucleotide primers and a molecular probe for the detection of fungal pathogens belonging to the genus Botrytis with sequences No. 1, 2 and 3 shown in the sequence listing. The invention also relates to a method for detecting a phytopathogenic fungus of the genus Botrytis, wherein the qPCR reaction uses the above-mentioned pair of primers and a molecular probe.

PL431985A 2019-11-28 2019-11-28 Oligonucleotide primers and molecular probe for detecting fitopathogenic botrytis sp. fungus and method for its detection PL239135B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL431985A PL239135B1 (en) 2019-11-28 2019-11-28 Oligonucleotide primers and molecular probe for detecting fitopathogenic botrytis sp. fungus and method for its detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL431985A PL239135B1 (en) 2019-11-28 2019-11-28 Oligonucleotide primers and molecular probe for detecting fitopathogenic botrytis sp. fungus and method for its detection

Publications (2)

Publication Number Publication Date
PL431985A1 true PL431985A1 (en) 2021-05-31
PL239135B1 PL239135B1 (en) 2021-11-08

Family

ID=76133048

Family Applications (1)

Application Number Title Priority Date Filing Date
PL431985A PL239135B1 (en) 2019-11-28 2019-11-28 Oligonucleotide primers and molecular probe for detecting fitopathogenic botrytis sp. fungus and method for its detection

Country Status (1)

Country Link
PL (1) PL239135B1 (en)

Also Published As

Publication number Publication date
PL239135B1 (en) 2021-11-08

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