PL431987A1 - Oligonucleotide primers and molecular probe for detecting fitopathogenic verticillium sp. fungus and method for its detection - Google Patents

Oligonucleotide primers and molecular probe for detecting fitopathogenic verticillium sp. fungus and method for its detection

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Publication number
PL431987A1
PL431987A1 PL431987A PL43198719A PL431987A1 PL 431987 A1 PL431987 A1 PL 431987A1 PL 431987 A PL431987 A PL 431987A PL 43198719 A PL43198719 A PL 43198719A PL 431987 A1 PL431987 A1 PL 431987A1
Authority
PL
Poland
Prior art keywords
detecting
molecular probe
verticillium
fungus
oligonucleotide primers
Prior art date
Application number
PL431987A
Other languages
Polish (pl)
Other versions
PL239137B1 (en
Inventor
Jacek Panek
Magdalena Frąc
Dominika Malarczyk
Original Assignee
Instytut Agrofizyki Im.Bohdana Dobrzańskiego Polskiej Akademii Nauk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Agrofizyki Im.Bohdana Dobrzańskiego Polskiej Akademii Nauk filed Critical Instytut Agrofizyki Im.Bohdana Dobrzańskiego Polskiej Akademii Nauk
Priority to PL431987A priority Critical patent/PL239137B1/en
Publication of PL431987A1 publication Critical patent/PL431987A1/en
Publication of PL239137B1 publication Critical patent/PL239137B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem wynalazku są startery oligonukleotydowe oraz sonda molekularna do wykrywania patogenów grzybowych należących do rodzaju Verticillium o sekwencjach nr 1, 2 i 3. Przedmiotem wynalazku jest również sposób wykrywania fitopatogenicznego grzyba z rodzaju Verticillium z zastosowaniem w reakcji qPCR powyższej pary starterów i sondy molekularnej.The invention relates to oligonucleotide primers and a molecular probe for detecting fungal pathogens belonging to the genus Verticillium with sequences No. 1, 2 and 3. The invention also relates to a method for detecting a phytopathogenic fungus of the genus Verticillium using the above pair of primers and a molecular probe in a qPCR reaction.

PL431987A 2019-11-28 2019-11-28 Oligonucleotide primers and molecular probe for detecting fitopathogenic verticillium sp. fungus and method for its detection PL239137B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL431987A PL239137B1 (en) 2019-11-28 2019-11-28 Oligonucleotide primers and molecular probe for detecting fitopathogenic verticillium sp. fungus and method for its detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL431987A PL239137B1 (en) 2019-11-28 2019-11-28 Oligonucleotide primers and molecular probe for detecting fitopathogenic verticillium sp. fungus and method for its detection

Publications (2)

Publication Number Publication Date
PL431987A1 true PL431987A1 (en) 2021-05-31
PL239137B1 PL239137B1 (en) 2021-11-08

Family

ID=76133073

Family Applications (1)

Application Number Title Priority Date Filing Date
PL431987A PL239137B1 (en) 2019-11-28 2019-11-28 Oligonucleotide primers and molecular probe for detecting fitopathogenic verticillium sp. fungus and method for its detection

Country Status (1)

Country Link
PL (1) PL239137B1 (en)

Also Published As

Publication number Publication date
PL239137B1 (en) 2021-11-08

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