PL438754A1 - Pair of oligonucleotide primers for detection and method for detecting the allele of the dominat crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.) - Google Patents
Pair of oligonucleotide primers for detection and method for detecting the allele of the dominat crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.) Download PDFInfo
- Publication number
- PL438754A1 PL438754A1 PL438754A PL43875421A PL438754A1 PL 438754 A1 PL438754 A1 PL 438754A1 PL 438754 A PL438754 A PL 438754A PL 43875421 A PL43875421 A PL 43875421A PL 438754 A1 PL438754 A1 PL 438754A1
- Authority
- PL
- Poland
- Prior art keywords
- avena
- pair
- avena sativa
- pendek
- subline
- Prior art date
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- 235000007319 Avena orientalis Nutrition 0.000 title abstract 5
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 108700028369 Alleles Proteins 0.000 title abstract 3
- 235000004535 Avena sterilis Nutrition 0.000 title abstract 3
- 241001647031 Avena sterilis Species 0.000 title abstract 3
- 239000003155 DNA primer Substances 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 3
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 244000075850 Avena orientalis Species 0.000 title 3
- 241000196324 Embryophyta Species 0.000 title 1
- 241000209763 Avena sativa Species 0.000 abstract 4
- 239000012634 fragment Substances 0.000 abstract 2
- 239000013615 primer Substances 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Przedmiot wynalazku stanowi para oligonukleotydowych starterów do wykrywania allelu dominującego genu odporności na rdzę koronową z sublinii formy mieszańcowej Avena sativa 'Pendek' × Avena sterilis CW-486 w roślinach owsa o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji. Przedmiotem wynalazku jest ponadto sposób identyfikacji allelu dominującego genu odporności na rdzę koronową z sublinii formy mieszańcowej Avena sativa 'Pendek' × Avena sterilis CW-486 w roślinach owsa, w którym polimorficzny fragment DNA sprzężony z badanym genem amplifikowany jest w reakcji PCR z zastosowaniem pary starterów, po czym dokonuje się detekcji produktu amplifikacji, a parę starterów stanowi para oligonukleotydowych starterów o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji, przy czym stosuje się marker 978. W wyniku PCR amplifikowany jest fragment DNA o długości 57 par zasad o sekwencji nr 3 przedstawionej na liście sekwencji.The subject of the invention is a pair of oligonucleotide primers for the detection of the dominant allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' × Avena sterilis CW-486 in oat plants with sequences No. 1 and 2 presented in the sequence list. The subject of the invention is also a method for identifying the dominant allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' × Avena sterilis CW-486 in oat plants, in which the polymorphic DNA fragment coupled with the test gene is amplified in a PCR reaction using a pair of primers followed by detection of the amplification product, and the primer pair is a pair of oligonucleotide primers with sequences #1 and 2 shown in the sequence listing using marker 978. The PCR amplified DNA fragment of 57 base pairs with sequence #3 shown in the sequence list.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL438754A PL243631B1 (en) | 2021-08-16 | 2021-08-16 | A pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant gene for resistance to crown rust from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL438754A PL243631B1 (en) | 2021-08-16 | 2021-08-16 | A pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant gene for resistance to crown rust from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL438754A1 true PL438754A1 (en) | 2023-02-20 |
| PL243631B1 PL243631B1 (en) | 2023-09-18 |
Family
ID=85225410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL438754A PL243631B1 (en) | 2021-08-16 | 2021-08-16 | A pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant gene for resistance to crown rust from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.) |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL243631B1 (en) |
-
2021
- 2021-08-16 PL PL438754A patent/PL243631B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL243631B1 (en) | 2023-09-18 |
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