PL438757A1 - Pair of oligonucleotide primers for detection and method for detecting the allele of the recessive crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.) - Google Patents

Pair of oligonucleotide primers for detection and method for detecting the allele of the recessive crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.) Download PDF

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PL438757A1
PL438757A1 PL438757A PL43875721A PL438757A1 PL 438757 A1 PL438757 A1 PL 438757A1 PL 438757 A PL438757 A PL 438757A PL 43875721 A PL43875721 A PL 43875721A PL 438757 A1 PL438757 A1 PL 438757A1
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Poland
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avena
pair
avena sativa
pendek
subline
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PL438757A
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Polish (pl)
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PL243634B1 (en
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Sylwia Sowa
Edyta Paczos-Grzęda
Joanna Toporowska
Aneta Koroluk
Krzysztof Kowalczyk
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Uniwersytet Przyrodniczy W Lublinie
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Priority to PL438757A priority Critical patent/PL243634B1/en
Publication of PL438757A1 publication Critical patent/PL438757A1/en
Publication of PL243634B1 publication Critical patent/PL243634B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiot wynalazku stanowi para oligonukleotydowych starterów do wykrywania allelu recesywnego genu odporności na rdzę koronową z sublinii formy mieszańcowej Avena sativa 'Pendek' x Avena sterilis CW-486 w roślinach owsa o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji. Przedmiotem wynalazku jest ponadto sposób identyfikacji allelu recesywnego genu odporności na rdzę koronową z sublinii formy mieszańcowej Avena sativa 'Pendek' x Avena sterilis CW-486 w roślinach owsa, w którym polimorficzny fragment DNA sprzężony z badanym genem amplifikowany jest w reakcji PCR z zastosowaniem pary starterów, po czym dokonuje się detekcji produktu amplifikacji, a parę starterów stanowi para oligonukleotydowych starterów o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji, przy czym stosuje się marker 370. W wyniku PCR amplifikowany jest fragment DNA o długości 55 par zasad o sekwencji nr 3 przedstawionej na liście sekwencji.The subject of the invention is a pair of oligonucleotide primers for the detection of the recessive allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants with sequences No. 1 and 2 presented in the sequence list. The subject of the invention is also a method for identifying the recessive allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants, in which the polymorphic DNA fragment coupled with the test gene is amplified in a PCR reaction using a pair of primers followed by detection of the amplification product and the primer pair is a pair of oligonucleotide primers of sequences #1 and 2 shown in the sequence listing using marker 370. PCR amplified a 55 bp DNA fragment of sequence #3 shown in the sequence list.

PL438757A 2021-08-16 2021-08-16 A pair of oligonucleotide primers for the detection and method of detecting the recessive allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.) PL243634B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL438757A PL243634B1 (en) 2021-08-16 2021-08-16 A pair of oligonucleotide primers for the detection and method of detecting the recessive allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL438757A PL243634B1 (en) 2021-08-16 2021-08-16 A pair of oligonucleotide primers for the detection and method of detecting the recessive allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.)

Publications (2)

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PL438757A1 true PL438757A1 (en) 2023-02-20
PL243634B1 PL243634B1 (en) 2023-09-18

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PL438757A PL243634B1 (en) 2021-08-16 2021-08-16 A pair of oligonucleotide primers for the detection and method of detecting the recessive allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in oat plants (Avena sativa L.)

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