PL449128A1 - A pair of oligonucleotide primers for detection and a method for detecting the dominant and recessive allele of the crown rust resistance gene from a subline isolated from the Iowa X270/X434 line obtained by crossing Avena sterilis Wahl No. 8 x Avena sativa in common oat (Avena sativa L.) plants - Google Patents

A pair of oligonucleotide primers for detection and a method for detecting the dominant and recessive allele of the crown rust resistance gene from a subline isolated from the Iowa X270/X434 line obtained by crossing Avena sterilis Wahl No. 8 x Avena sativa in common oat (Avena sativa L.) plants

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Publication number
PL449128A1
PL449128A1 PL449128A PL44912824A PL449128A1 PL 449128 A1 PL449128 A1 PL 449128A1 PL 449128 A PL449128 A PL 449128A PL 44912824 A PL44912824 A PL 44912824A PL 449128 A1 PL449128 A1 PL 449128A1
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PL
Poland
Prior art keywords
pair
avena
dominant
subline
wahl
Prior art date
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PL449128A
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Polish (pl)
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PL248968B1 (en
Inventor
Edyta Paczos-Grzęda
Joanna Lech
Sylwia Sowa
Aneta Koroluk
Krzysztof Kowalczyk
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Uniwersytet Przyrodniczy W Lublinie
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Application filed by Uniwersytet Przyrodniczy W Lublinie filed Critical Uniwersytet Przyrodniczy W Lublinie
Priority to PL449128A priority Critical patent/PL248968B1/en
Publication of PL449128A1 publication Critical patent/PL449128A1/en
Publication of PL248968B1 publication Critical patent/PL248968B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiot wynalazku stanowi para oligonukleotydowych starterów do wykrywania allelu dominującego i recesywnego genu odporności na rdzę koronową z sublinii wyodrębnionej z linii Iowa X270/X434, uzyskanej w wyniku krzyżowania A. sterilis Wahl No. 8 x A. sativa w roślinach owsa o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji. Przedmiotem wynalazku jest ponadto sposób identyfikacji allelu dominującego i recesywnego genu odporności na rdzę koronową z sublinii wyodrębnionej z linii Iowa X270/X434, uzyskanej w wyniku krzyżowania A. sterilis Wahl No. 8 x A. sativa w roślinach owsa, w którym polimorficzny fragment DNA sprzężony z badanym genem amplifikowany jest w reakcji PCR z zastosowaniem pary starterów, po czym dokonuje się detekcji produktu amplifikacji, a parę starterów stanowi para oligonukleotydów o sekwencjach nr 1 i 2 przedstawionych na liście sekwencji, przy czym stosuje się marker 821. W wyniku PCR amplifikowany jest fragment DNA o długości 106 par zasad o sekwencji nr 3 przedstawionej na liście sekwencji w obecności allelu dominującego lub fragmentu DNA o długości 115 par zasad o sekwencji nr 4, przedstawionej na liście sekwencji w obecności allelu recesywnego.The invention is a pair of oligonucleotide primers for detecting the dominant and recessive alleles of the crown rust resistance gene from a subline isolated from the Iowa X270/X434 line, obtained by crossing A. sterilis Wahl No. 8 x A. sativa in oat plants with sequences no. 1 and 2 presented in the sequence listing. The invention also provides a method for identifying the dominant and recessive alleles of the crown rust resistance gene from a subline isolated from the Iowa X270/X434 line, obtained by crossing A. sterilis Wahl No. 8 x A. sativa in oat plants, in which a polymorphic DNA fragment linked to the gene under study is amplified in a PCR reaction using a pair of primers, followed by detection of the amplification product, and the pair of primers is a pair of oligonucleotides with sequences no. 1 and 2 presented in the sequence list, where marker 821 is used. As a result of PCR, a DNA fragment of 106 base pairs in length is amplified with sequence no. 3 presented in the sequence list in the presence of a dominant allele or a DNA fragment of 115 base pairs in length with sequence no. 4 presented in the sequence list in the presence of a recessive allele.

PL449128A 2024-07-03 2024-07-03 A pair of oligonucleotide primers for detection and a method for detecting the dominant and recessive allele of the crown rust resistance gene from a subline isolated from the Iowa X270/X434 line obtained by crossing Avena sterilis Wahl No. 8 x Avena sativa in common oat (Avena sativa L.) plants PL248968B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL449128A PL248968B1 (en) 2024-07-03 2024-07-03 A pair of oligonucleotide primers for detection and a method for detecting the dominant and recessive allele of the crown rust resistance gene from a subline isolated from the Iowa X270/X434 line obtained by crossing Avena sterilis Wahl No. 8 x Avena sativa in common oat (Avena sativa L.) plants

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL449128A PL248968B1 (en) 2024-07-03 2024-07-03 A pair of oligonucleotide primers for detection and a method for detecting the dominant and recessive allele of the crown rust resistance gene from a subline isolated from the Iowa X270/X434 line obtained by crossing Avena sterilis Wahl No. 8 x Avena sativa in common oat (Avena sativa L.) plants

Publications (2)

Publication Number Publication Date
PL449128A1 true PL449128A1 (en) 2024-12-30
PL248968B1 PL248968B1 (en) 2026-02-16

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ID=98772964

Family Applications (1)

Application Number Title Priority Date Filing Date
PL449128A PL248968B1 (en) 2024-07-03 2024-07-03 A pair of oligonucleotide primers for detection and a method for detecting the dominant and recessive allele of the crown rust resistance gene from a subline isolated from the Iowa X270/X434 line obtained by crossing Avena sterilis Wahl No. 8 x Avena sativa in common oat (Avena sativa L.) plants

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PL (1) PL248968B1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL233477B1 (en) * 2017-08-11 2019-10-31 Univ Przyrodniczy W Lublinie A pair of starter oligonucleotides for detecting, and the method for detecting of the recessive allele of immunity gene Pc39 to crown rust in the plants of common oat (Avena sativa L.)
PL233478B1 (en) * 2017-08-11 2019-10-31 Univ Przyrodniczy W Lublinie A pair of starter oligonucleotides for detecting, and the method for detecting of the dominant allele of immunity gene to crown rust Pc39 in the plants of common oat (Avena sativa L.)
PL243632B1 (en) * 2021-08-16 2023-09-18 Univ Przyrodniczy W Lublinie A pair of oligonucleotide primers for detection and a method for detecting the presence of the dominant and recessive allele of the crown rust resistance gene from the subline of the hybrid form Avena sativa 'Pendek' x Avena sterilis CW-486 in the genome of common oats (Avena sativa L.)
PL244887B1 (en) * 2022-04-13 2024-03-18 Univ Przyrodniczy W Lublinie A pair of oligonucleotide primers for the detection and method of detecting the allele of the dominant crown rust resistance gene from the subline of the hybrid form A. sterilis PI 296244 x A. sativa TAM-O-312 in oat plants (Avena sativa L.)

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Publication number Publication date
PL248968B1 (en) 2026-02-16

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