PL442186A1 - Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów - Google Patents

Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów

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Publication number
PL442186A1
PL442186A1 PL442186A PL44218622A PL442186A1 PL 442186 A1 PL442186 A1 PL 442186A1 PL 442186 A PL442186 A PL 442186A PL 44218622 A PL44218622 A PL 44218622A PL 442186 A1 PL442186 A1 PL 442186A1
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Poland
Prior art keywords
nucleoside
nucleotide
oligonucleotide library
application
subject
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PL442186A
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English (en)
Inventor
Agnieszka SOK-GROCHOWSKA
Madga Joanna Puchała
Barbara KUCHARSKA
Piotr JAKIMOWICZ
Monika CZARNECKA
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Pure Biologics Spółka Akcyjna
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Priority to PL442186A priority Critical patent/PL442186A1/pl
Priority to PCT/PL2023/050071 priority patent/WO2024049312A2/en
Publication of PL442186A1 publication Critical patent/PL442186A1/pl

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/335—Modified T or U
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)

Abstract

Pierwszym przedmiotem zgłoszenia jest sposób syntezy i oczyszczania nukleozydu i/lub nukleotydu. Drugim przedmiotem zgłoszenia jest zmodyfikowany nukleozyd i/lub nukleotyd, stanowiący mono-, di- lub trójfosforan, zawierający jako zasadę azotową pochodną cytozyny lub uracylu, która w pozycji 5' pierścienia heterocyklicznego posiada grupę 1,2,3-triazolową lub łańcuch alkanowy lub alkinowy. Trzecim przedmiotem zgłoszenia jest cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydowy. Czwartym przedmiotem zgłoszenia jest biblioteka oligonukleotydów posiadająca w sekwencji region z sekwencją dowolną o długości co najmniej 10 nukleotydów, oraz flankujące go dwa regiony o sekwencjach stałych o długości co najmniej 10 nukleotydów lub pozbawiona regionów flankujących. Kolejnym przedmiotem zgłoszenia jest zastosowanie biblioteki oligonukleotydów do otrzymywania aptamerów techniką SELEX i pochodnymi.
PL442186A 2022-09-02 2022-09-02 Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów PL442186A1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL442186A PL442186A1 (pl) 2022-09-02 2022-09-02 Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów
PCT/PL2023/050071 WO2024049312A2 (en) 2022-09-02 2023-09-01 A method for the synthesis and purification of a nucleotide and/or a nucleoside, a modified nucleotide and/or a nucleoside, a dna molecule containing a single- or double-stranded oligonucleotide chain, an oligonucleotide library, use of the oligonucleotide library

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL442186A PL442186A1 (pl) 2022-09-02 2022-09-02 Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów

Publications (1)

Publication Number Publication Date
PL442186A1 true PL442186A1 (pl) 2024-03-04

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PL442186A PL442186A1 (pl) 2022-09-02 2022-09-02 Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów

Country Status (2)

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PL (1) PL442186A1 (pl)
WO (1) WO2024049312A2 (pl)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6884882B1 (en) * 2004-01-07 2005-04-26 Postech Foundation 2′-deoxyuridine derivatives and hydrogels formed therewith
PL239946B1 (pl) * 2015-09-17 2022-01-31 Pure Biologics Spolka Z Ograniczona Odpowiedzialnoscia Sposób syntezy i oczyszczania nukleotydu, zmodyfikowany nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6884882B1 (en) * 2004-01-07 2005-04-26 Postech Foundation 2′-deoxyuridine derivatives and hydrogels formed therewith
PL239946B1 (pl) * 2015-09-17 2022-01-31 Pure Biologics Spolka Z Ograniczona Odpowiedzialnoscia Sposób syntezy i oczyszczania nukleotydu, zmodyfikowany nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SIEGL J, NIKOLIN C, PHUNG NL, THOMS S, BLUME C, MAYER G., SPLIT-COMBINE CLICK-SELEX REVEALS LIGANDS RECOGNIZING THE TRANSPLANT REJECTION BIOMARKER CXCL9. ACS CHEM BIOL. 2022 JAN 21;17(1):129-137. DOI: 10.1021/ACSCHEMBIO.1C00789 *

Also Published As

Publication number Publication date
WO2024049312A3 (en) 2024-05-02
WO2024049312A2 (en) 2024-03-07

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