PL442186A1 - Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów - Google Patents

Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów

Info

Publication number
PL442186A1
PL442186A1 PL442186A PL44218622A PL442186A1 PL 442186 A1 PL442186 A1 PL 442186A1 PL 442186 A PL442186 A PL 442186A PL 44218622 A PL44218622 A PL 44218622A PL 442186 A1 PL442186 A1 PL 442186A1
Authority
PL
Poland
Prior art keywords
nucleoside
nucleotide
oligonucleotide library
application
subject
Prior art date
Application number
PL442186A
Other languages
English (en)
Inventor
Agnieszka SOK-GROCHOWSKA
Madga Joanna Puchała
Barbara KUCHARSKA
Piotr JAKIMOWICZ
Monika CZARNECKA
Original Assignee
Pure Biologics Spółka Akcyjna
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pure Biologics Spółka Akcyjna filed Critical Pure Biologics Spółka Akcyjna
Priority to PL442186A priority Critical patent/PL442186A1/pl
Priority to PCT/PL2023/050071 priority patent/WO2024049312A2/en
Publication of PL442186A1 publication Critical patent/PL442186A1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/335—Modified T or U
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)

Abstract

Pierwszym przedmiotem zgłoszenia jest sposób syntezy i oczyszczania nukleozydu i/lub nukleotydu. Drugim przedmiotem zgłoszenia jest zmodyfikowany nukleozyd i/lub nukleotyd, stanowiący mono-, di- lub trójfosforan, zawierający jako zasadę azotową pochodną cytozyny lub uracylu, która w pozycji 5' pierścienia heterocyklicznego posiada grupę 1,2,3-triazolową lub łańcuch alkanowy lub alkinowy. Trzecim przedmiotem zgłoszenia jest cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydowy. Czwartym przedmiotem zgłoszenia jest biblioteka oligonukleotydów posiadająca w sekwencji region z sekwencją dowolną o długości co najmniej 10 nukleotydów, oraz flankujące go dwa regiony o sekwencjach stałych o długości co najmniej 10 nukleotydów lub pozbawiona regionów flankujących. Kolejnym przedmiotem zgłoszenia jest zastosowanie biblioteki oligonukleotydów do otrzymywania aptamerów techniką SELEX i pochodnymi.
PL442186A 2022-09-02 2022-09-02 Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów PL442186A1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL442186A PL442186A1 (pl) 2022-09-02 2022-09-02 Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów
PCT/PL2023/050071 WO2024049312A2 (en) 2022-09-02 2023-09-01 A method for the synthesis and purification of a nucleotide and/or a nucleoside, a modified nucleotide and/or a nucleoside, a dna molecule containing a single- or double-stranded oligonucleotide chain, an oligonucleotide library, use of the oligonucleotide library

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL442186A PL442186A1 (pl) 2022-09-02 2022-09-02 Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów

Publications (1)

Publication Number Publication Date
PL442186A1 true PL442186A1 (pl) 2024-03-04

Family

ID=88647543

Family Applications (1)

Application Number Title Priority Date Filing Date
PL442186A PL442186A1 (pl) 2022-09-02 2022-09-02 Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów

Country Status (2)

Country Link
PL (1) PL442186A1 (pl)
WO (1) WO2024049312A2 (pl)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6884882B1 (en) * 2004-01-07 2005-04-26 Postech Foundation 2′-deoxyuridine derivatives and hydrogels formed therewith
PL239946B1 (pl) * 2015-09-17 2022-01-31 Pure Biologics Spolka Z Ograniczona Odpowiedzialnoscia Sposób syntezy i oczyszczania nukleotydu, zmodyfikowany nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6884882B1 (en) * 2004-01-07 2005-04-26 Postech Foundation 2′-deoxyuridine derivatives and hydrogels formed therewith
PL239946B1 (pl) * 2015-09-17 2022-01-31 Pure Biologics Spolka Z Ograniczona Odpowiedzialnoscia Sposób syntezy i oczyszczania nukleotydu, zmodyfikowany nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SIEGL J, NIKOLIN C, PHUNG NL, THOMS S, BLUME C, MAYER G., SPLIT-COMBINE CLICK-SELEX REVEALS LIGANDS RECOGNIZING THE TRANSPLANT REJECTION BIOMARKER CXCL9. ACS CHEM BIOL. 2022 JAN 21;17(1):129-137. DOI: 10.1021/ACSCHEMBIO.1C00789 *

Also Published As

Publication number Publication date
WO2024049312A3 (en) 2024-05-02
WO2024049312A2 (en) 2024-03-07

Similar Documents

Publication Publication Date Title
ES2302701T3 (es) N3'-p5' tiofosforamidatos oligonucleotidicos: su sintesis y uso.
Ménová et al. Scope and limitations of the nicking enzyme amplification reaction for the synthesis of base-modified oligonucleotides and primers for PCR
AU2024266967A1 (en) A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides
JP3893057B2 (ja) 新規な核酸塩基対
JP2012095665A (ja) ２’−ニトロベンジル改変型リボヌクレオチド
JP2018088924A5 (pl)
JP2016540755A (ja) シチジン−５−カルボキサミド修飾ヌクレオチド組成物及びそれに関連する方法
Sochacka et al. 2-Thiouracil deprived of thiocarbonyl function preferentially base pairs with guanine rather than adenine in RNA and DNA duplexes
FI115400B (fi) Menetelmä oligoribonukleotidi- ja ribotsyymianalogien valmistamiseksi, joissa on terminaaliset 3 '-3'- tai 5'-5'-sidokset
Egli Nucleic acid crystallography: current progress
JP2021503003A (ja) アミン保護された部分を含むヌクレオチド誘導体、並びに鋳型依存性及び鋳型非依存性酵素的核酸合成におけるそれらの使用
CA3214113A1 (en) Modified nucleosides
JPH10195098A (ja) 新規ヌクレオチド類縁体
CA2508468A1 (en) Nucleosides or nucleotides having novel unnatural bases and use thereof
Obika et al. Triplex formation by an oligonucleotide containing conformationally locked C-nucleoside, 5-(2-O, 4-C-methylene-β-D-ribofuranosyl) oxazole
Matyašovský et al. 2-Substituted 2′-deoxyinosine 5′-triphosphates as substrates for polymerase synthesis of minor-groove-modified DNA and effects on restriction endonuclease cleavage
Ohno et al. Synthesis of photocaged 6-O-(2-nitrobenzyl) guanosine and 4-O-(2-nitrobenzyl) uridine triphosphates for photocontrol of the RNA transcription reaction
CN105899525A (zh) 光交联的形成抑制方法、及自交联体形成抑制型光响应性核酸
WO2024049312A3 (en) A method for the synthesis and purification of a nucleotide and/or a nucleoside, a modified nucleotide and/or a nucleoside, a dna molecule containing a single- or double-stranded oligonucleotide chain, an oligonucleotide library, use of the oligonucleotide library
CN115003680B (zh) 制备核酸寡聚物的方法
Beck et al. Double-headed nucleic acids condense the molecular information of DNA to half the number of nucleotides
Nagatsugi et al. Selective reaction to a flipping cytidine of the duplex DNA mediated by triple helix formation
Srivastava et al. Achiral, acyclic nucleic acids: synthesis and biophysical studies of a possible prebiotic polymer
Kasahara et al. Artificial specific binders directly recovered from chemically modified nucleic acid libraries
Maier et al. Postsynthetic guanidinylation of primary amino groups in the minor and major grooves of oligonucleotides