PT2970912T - Métodos e materiais para a diferenciação hematoendotelial de células estaminais pluripotentes humanas em condições definidas - Google Patents

Métodos e materiais para a diferenciação hematoendotelial de células estaminais pluripotentes humanas em condições definidas

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Publication number
PT2970912T
PT2970912T PT14722013T PT14722013T PT2970912T PT 2970912 T PT2970912 T PT 2970912T PT 14722013 T PT14722013 T PT 14722013T PT 14722013 T PT14722013 T PT 14722013T PT 2970912 T PT2970912 T PT 2970912T
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Portugal
Prior art keywords
hematoendotelial
differentiation
materials
methods
human pluripotent
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PT14722013T
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English (en)
Inventor
Slukvin Igor
Ichiro Uenishi Gene
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Wisconsin Alumni Res Found
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Publication of PT2970912T publication Critical patent/PT2970912T/pt

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    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/13Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/54Collagen; Gelatin

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PT14722013T 2013-03-13 2014-03-12 Métodos e materiais para a diferenciação hematoendotelial de células estaminais pluripotentes humanas em condições definidas PT2970912T (pt)

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US (3) US9938499B2 (pt)
EP (1) EP2970912B1 (pt)
JP (1) JP6677631B2 (pt)
KR (1) KR102151210B1 (pt)
CN (1) CN105209609B (pt)
AU (1) AU2014248651B2 (pt)
BR (1) BR112015022770B1 (pt)
CA (2) CA3141731A1 (pt)
DK (1) DK2970912T3 (pt)
ES (1) ES2720196T3 (pt)
HU (1) HUE043785T2 (pt)
IL (1) IL241286B (pt)
MX (1) MX366900B (pt)
PL (1) PL2970912T3 (pt)
PT (1) PT2970912T (pt)
SG (1) SG11201507275TA (pt)
TR (1) TR201904270T4 (pt)
WO (1) WO2014165131A1 (pt)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434527A (zh) 2006-04-14 2017-02-22 安斯泰来再生医药协会 血管瘤集落形成细胞
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
HUE043785T2 (hu) 2013-03-13 2019-09-30 Wisconsin Alumni Res Found Módszerek és anyagok humán pluripotens õssejtek hematoendothelialis differenciálására meghatározott körülmények között
US9868939B2 (en) 2013-06-12 2018-01-16 Wisconsin Alumni Research Foundation Generating vasculogenic cell populations
SG10201801654RA (en) 2014-05-28 2018-04-27 Childrens Hospital Med Ct Methods and systems for converting precursor cells into gastric tissues through directed differentiation
AU2015331848B2 (en) 2014-10-17 2022-03-03 Children's Hospital Medical Center, D/B/A Cincinnati Children's Hospital Medical Center In vivo model of human small intestine using pluripotent stem cells and methods of making and using same
US11220672B2 (en) * 2014-12-31 2022-01-11 Wisconsin Alumni Research Foundation Human pluripotent stem cell-based system for generating endothelial cells
WO2016123100A1 (en) * 2015-01-26 2016-08-04 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
SG11201706706XA (en) * 2015-02-20 2017-09-28 Wisconsin Alumni Res Found Generating arterial endothelial cell populations
US10787640B2 (en) 2015-03-03 2020-09-29 The Board Of Trustees Of The Leland Stanford Junior University Producing mesodermal cell types and methods of using the same
CN104660703B (zh) * 2015-03-05 2017-12-15 国家电网公司 多网络融合用电信息采集系统及最优通信网络的选择方法
WO2016143826A1 (ja) * 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法
WO2017004169A1 (en) * 2015-06-29 2017-01-05 Northeastern University Dendritic cell generator
JP6646311B2 (ja) * 2015-07-17 2020-02-14 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
US10947502B2 (en) * 2015-10-20 2021-03-16 FUJIFILM Cellular Dynamics, Inc. Methods for directed differentiation of pluripotent stem cells to immune cells
KR20250141836A (ko) 2015-11-04 2025-09-29 페이트 세러퓨틱스, 인코포레이티드 만능 세포의 유전자 조작
SG11201803145RA (en) * 2015-11-04 2018-05-30 Fate Therapeutics Inc Methods and compositions for inducing hematopoietic cell differentiation
US11174461B2 (en) * 2016-03-16 2021-11-16 Cynata Therapeutics Limited Colony forming medium and use thereof
WO2017181168A1 (en) * 2016-04-15 2017-10-19 Angiocrine Bioscience, Inc. Enhanced gene delivery methods
CN109415685B (zh) 2016-05-05 2023-07-04 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
CN109153970A (zh) * 2016-05-17 2019-01-04 新加坡科技研究局 人类干细胞衍生的内皮细胞、内皮-肝细胞共培养系统以及其用途
WO2018037091A1 (en) * 2016-08-24 2018-03-01 Hi-Stem Ggmbh Methods for the identification and isolation of hematopoietic stem and progenitor cells
US11525119B2 (en) 2016-09-06 2022-12-13 The Children's Medical Center Corporation Immune cells derived from induced pluripotent stem cell
AU2017340644B2 (en) * 2016-10-05 2024-05-09 FUJIFILM Cellular Dynamics, Inc. Methods for directed differentiation of pluripotent stem cells to HLA homozygous immune cells
JP7248571B2 (ja) 2016-10-05 2023-03-29 フジフィルム セルラー ダイナミクス,インコーポレイテッド MeCP2が破壊された誘導多能性幹細胞からの成熟系列の生成
EP4553082A3 (en) 2016-11-04 2025-08-20 Children's Hospital Medical Center Liver organoid compositions and methods of making and using same
EP3548507A4 (en) 2016-12-05 2020-07-15 Children's Hospital Medical Center COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF
AU2018240416B2 (en) * 2017-03-21 2024-07-11 Centre National De La Recherche Scientifique Methods of improving hematopoietic grafts
CN106867961A (zh) * 2017-03-27 2017-06-20 广州润虹医药科技有限公司 一种诱导多能干细胞形成中胚层细胞的诱导培养基及诱导方法
RU2019133327A (ru) * 2017-04-07 2021-05-07 Сината Терапьютикс Лимитед Способ лечения побочного эффекта терапии Т-клетками с химерными антигенными рецепторами (CAR)
JP7248586B2 (ja) 2017-04-14 2023-03-29 チルドレンズ ホスピタル メディカル センター 複数ドナー幹細胞組成物およびそれを作製する方法
SG11201909331UA (en) 2017-04-18 2019-11-28 Fujifilm Cellular Dynamics Inc Antigen-specific immune effector cells
JP2020523400A (ja) * 2017-06-16 2020-08-06 サイナータ セラピューティクス リミテッド 免疫療法の副作用を治療するための方法
EP3638773A1 (en) * 2017-06-16 2020-04-22 IMBA-Institut für Molekulare Biotechnologie GmbH Blood vessel organoid, methods of producing and using said organoids
JP2020532992A (ja) * 2017-09-07 2020-11-19 ステモニックス インコーポレイティド 胚体内胚葉へのヒト幹細胞の効率的な分化
KR20200053501A (ko) 2017-09-15 2020-05-18 시나타 세라퓨틱스 엘티디 알레르기성 기도 질환 (aad)/ 천식을 치료하는 방법
EP3694603B1 (en) 2017-10-10 2026-04-08 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
SG11202003790PA (en) 2017-10-31 2020-05-28 Murdoch Childrens Res Inst Composition and method
US12037607B2 (en) 2017-11-08 2024-07-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of preparing hematopoietic progenitor cells in vitro
SG11202004680UA (en) 2017-11-22 2020-06-29 Mesoblast Int Sarl Cellular compositions and methods of treatment i
US11365390B2 (en) 2017-12-19 2022-06-21 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
JP2021509812A (ja) 2018-01-05 2021-04-08 プレートレット バイオジェネシス, インコーポレイテッド 巨核球を産生するための組成物および方法
EP3813853A4 (en) 2018-06-29 2022-04-06 Platelet Biogenesis, Inc. DELIVERY COMPOSITIONS AND METHODS OF USE
EP4678727A3 (en) 2018-07-26 2026-03-11 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
AU2019336221B2 (en) * 2018-09-07 2025-05-15 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
CA3112026A1 (en) 2018-09-12 2020-03-19 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
CN109334379B (zh) * 2018-09-19 2021-11-23 江苏大学 重型车用液电式惯容器非线性惯性力辨识方法
US10647954B1 (en) 2018-11-15 2020-05-12 Flaskworks, Llc Dendritic cell generating apparatus and method
CA3127593A1 (en) * 2019-01-22 2020-07-30 Washington University Compositions and methods for generating hematopoietic stem cells (hscs)
US12600943B2 (en) 2019-02-01 2026-04-14 The University Of Hong Kong Innervated organoid compositions and methods of making same
CN111154719B (zh) * 2019-02-28 2024-03-01 西湖生物医药科技(杭州)有限公司 利用外周血体外制备成熟红细胞的方法及制剂
AU2020283048A1 (en) 2019-05-31 2021-12-23 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
US12497597B2 (en) 2019-05-31 2025-12-16 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
AU2020325825B2 (en) 2019-08-05 2026-02-05 Mesoblast International Sarl Cellular compositions comprising viral vectors and methods of treatment
US11566217B2 (en) 2019-08-13 2023-01-31 Flaskworks, Llc Duty cycle for cell culture systems
GB201911953D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd T cell production fram RAG inacivated iPSCs
EP4017969A1 (en) * 2019-08-20 2022-06-29 Adaptimmune Limited Culture medium for haematopoietic induction
GB201911957D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd Methods of producing haemogenic progenitor cells from pluripotent stem cells
CN115003800A (zh) * 2019-08-28 2022-09-02 安斯泰来再生医药协会 治疗血管疾病的方法
CN114901799A (zh) 2019-10-21 2022-08-12 弗拉斯沃克斯有限责任公司 用于细胞培养的系统和方法
JP7654259B2 (ja) * 2019-10-29 2025-04-01 国立大学法人京都大学 多能性幹細胞から造血性内皮細胞および/または造血前駆細胞を製造する方法
US20230030773A1 (en) * 2019-12-16 2023-02-02 The Board Of Trustees Of The Leland Stanford Junior University Use of polyvinyl alcohol for cell culture of immune cells
JP7072756B2 (ja) * 2019-12-27 2022-05-23 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
CN113493767B (zh) * 2020-04-01 2024-04-26 北京大学 利用人多潜能干细胞体外制备嗜酸性粒细胞
CA3188492A1 (en) 2020-08-10 2022-02-17 Silviu Itescu Cellular compositions and methods of treatment
WO2022040798A1 (en) * 2020-08-25 2022-03-03 University Health Network Compositions and methods for generating human yolk sac-like hematopoietic cells
US11459372B2 (en) 2020-11-30 2022-10-04 Crispr Therapeutics Ag Gene-edited natural killer cells
WO2022144632A1 (en) 2020-12-30 2022-07-07 Crispr Therapeutics Ag Compositions and methods for differentiating stem cells into nk cells
CN112680410B (zh) * 2021-01-19 2022-08-02 上海爱萨尔生物科技有限公司 诱导多能干细胞分化培养心脏成纤维细胞的方法及其培养液
US20240043800A1 (en) * 2021-02-05 2024-02-08 Sungkwang Medical Foundation Method for differentiating pluripotent stem cell-derived hemogenic endothelial cells into lymphoid lineage cells
KR102766602B1 (ko) * 2021-02-05 2025-02-13 의료법인 성광의료재단 다능성 줄기세포 유래 조혈 내피세포를 이용한 유전자 편집된 세포의 제조방법
GB202102297D0 (en) * 2021-02-18 2021-04-07 Adaptimmune Ltd Methods of Producing Haemogenic Endothelial Cells from Pluripotent Stem Cells
CN113046318B (zh) * 2021-04-13 2023-04-18 中国科学院深圳先进技术研究院 一种诱导多能干细胞向造血前体细胞分化的培养基以及方法
WO2023019213A1 (en) * 2021-08-13 2023-02-16 Simcere Innovation, Inc. Three-dimensional culturing of pluripotent stem cells to produce hematopoietic stem cells
WO2023201361A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
JP2026505993A (ja) * 2023-02-16 2026-02-20 シンタックス バイオ,インク. 造血系に対する細胞プログラミングのためのシステムおよびその方法
TW202600811A (zh) * 2024-03-01 2026-01-01 史丹佛大學董事會 生成內皮細胞的方法
CN120173879A (zh) * 2025-05-13 2025-06-20 细胞生态海河实验室 一种由多能干细胞向造血干祖细胞分化的培养基组合物、其使用方法和应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE549395T1 (de) 2004-09-08 2012-03-15 Wisconsin Alumni Res Found Medium und kultur von embryonalen stammzellen
AU2006252576B2 (en) 2005-06-01 2011-01-20 Wisconsin Alumni Research Foundation Method of forming dendritic cells from embryonic stem cells
US8034613B2 (en) 2005-06-01 2011-10-11 Wisconsin Alumni Research Foundation Multipotent lymphohematopoietic progenitor cells
US20080070303A1 (en) 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
AU2007215276B2 (en) 2006-02-09 2013-09-19 Maryna E. Gumenyuk Erythroid cells producing adult-type beta-hemoglobin generated from human embryonic stem cells
JP5813321B2 (ja) 2007-03-23 2015-11-17 ウィスコンシン アラムニ リサーチ ファンデーション 体細胞の再プログラム化
US20110236971A2 (en) * 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
US7615374B2 (en) 2007-09-25 2009-11-10 Wisconsin Alumni Research Foundation Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
US8372643B2 (en) 2007-12-06 2013-02-12 Agency For Science, Technology And Research Method for extracellular matrix mediated differentiation and proliferation of stem cells
US20110086424A1 (en) * 2008-05-06 2011-04-14 Advanced Cell Technology, Inc. Methods for producing enucleated erythroid cells derived from pluripotent stem cells
KR101720961B1 (ko) 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
WO2010143529A1 (ja) * 2009-06-09 2010-12-16 国立大学法人名古屋大学 多能性幹細胞を胸腺上皮へ分化誘導する方法
EP2563908B1 (en) * 2010-04-25 2019-01-09 Icahn School of Medicine at Mount Sinai Generation of anterior foregut endoderm from pluripotent cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
HUE043785T2 (hu) 2013-03-13 2019-09-30 Wisconsin Alumni Res Found Módszerek és anyagok humán pluripotens õssejtek hematoendothelialis differenciálására meghatározott körülmények között

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