SE465576B - Fagvektor lambda sk9 framstaellning av genbanker - Google Patents
Fagvektor lambda sk9 framstaellning av genbankerInfo
- Publication number
- SE465576B SE465576B SE9001070A SE9001070A SE465576B SE 465576 B SE465576 B SE 465576B SE 9001070 A SE9001070 A SE 9001070A SE 9001070 A SE9001070 A SE 9001070A SE 465576 B SE465576 B SE 465576B
- Authority
- SE
- Sweden
- Prior art keywords
- phage
- dna
- fragment
- vector
- bam
- Prior art date
Links
- 239000013598 vector Substances 0.000 title claims abstract description 48
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 34
- 239000012634 fragment Substances 0.000 claims abstract description 74
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 65
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 claims abstract description 45
- 241000588724 Escherichia coli Species 0.000 claims abstract description 37
- 241001524679 Escherichia virus M13 Species 0.000 claims abstract description 12
- 238000003780 insertion Methods 0.000 claims abstract description 6
- 230000037431 insertion Effects 0.000 claims abstract description 6
- 238000011160 research Methods 0.000 claims abstract description 4
- 238000009395 breeding Methods 0.000 claims abstract description 3
- 230000001488 breeding effect Effects 0.000 claims abstract description 3
- 244000005700 microbiome Species 0.000 claims abstract description 3
- 238000001228 spectrum Methods 0.000 claims abstract description 3
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 241000701959 Escherichia virus Lambda Species 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 description 117
- 210000004027 cell Anatomy 0.000 description 55
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 29
- 229960000723 ampicillin Drugs 0.000 description 29
- 238000002360 preparation method Methods 0.000 description 20
- 102000004190 Enzymes Human genes 0.000 description 18
- 108090000790 Enzymes Proteins 0.000 description 18
- 239000013612 plasmid Substances 0.000 description 16
- 239000000243 solution Substances 0.000 description 16
- 238000001962 electrophoresis Methods 0.000 description 14
- 229920001817 Agar Polymers 0.000 description 12
- 239000008272 agar Substances 0.000 description 12
- 238000000034 method Methods 0.000 description 12
- 238000010561 standard procedure Methods 0.000 description 11
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 238000002844 melting Methods 0.000 description 9
- 229920000936 Agarose Polymers 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 8
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 7
- 102000004594 DNA Polymerase I Human genes 0.000 description 7
- 108010017826 DNA Polymerase I Proteins 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 102000012410 DNA Ligases Human genes 0.000 description 6
- 108010061982 DNA Ligases Proteins 0.000 description 6
- 230000009089 cytolysis Effects 0.000 description 6
- 230000007062 hydrolysis Effects 0.000 description 6
- 238000006460 hydrolysis reaction Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 5
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 5
- 241000701533 Escherichia virus T4 Species 0.000 description 5
- 241001135910 Phage M13mp18 Species 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 239000000020 Nitrocellulose Substances 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000008018 melting Effects 0.000 description 4
- 229920001220 nitrocellulos Polymers 0.000 description 4
- 230000003252 repetitive effect Effects 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 239000001226 triphosphate Substances 0.000 description 4
- 235000011178 triphosphate Nutrition 0.000 description 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 206010042566 Superinfection Diseases 0.000 description 3
- 239000011543 agarose gel Substances 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 3
- 241000702192 Escherichia virus P2 Species 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 241000702058 Phage 21 Species 0.000 description 2
- 238000005054 agglomeration Methods 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 230000001320 lysogenic effect Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 101150079601 recA gene Proteins 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- XINQFOMFQFGGCQ-UHFFFAOYSA-L (2-dodecoxy-2-oxoethyl)-[6-[(2-dodecoxy-2-oxoethyl)-dimethylazaniumyl]hexyl]-dimethylazanium;dichloride Chemical compound [Cl-].[Cl-].CCCCCCCCCCCCOC(=O)C[N+](C)(C)CCCCCC[N+](C)(C)CC(=O)OCCCCCCCCCCCC XINQFOMFQFGGCQ-UHFFFAOYSA-L 0.000 description 1
- VHJLVAABSRFDPM-UHFFFAOYSA-N 1,4-dithiothreitol Chemical compound SCC(O)C(O)CS VHJLVAABSRFDPM-UHFFFAOYSA-N 0.000 description 1
- JGSARLDLIJGVTE-UHFFFAOYSA-N 3,3-dimethyl-7-oxo-6-[(2-phenylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Chemical compound O=C1N2C(C(O)=O)C(C)(C)SC2C1NC(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-UHFFFAOYSA-N 0.000 description 1
- UXHQLGLGLZKHTC-CUNXSJBXSA-N 4-[(3s,3ar)-3-cyclopentyl-7-(4-hydroxypiperidine-1-carbonyl)-3,3a,4,5-tetrahydropyrazolo[3,4-f]quinolin-2-yl]-2-chlorobenzonitrile Chemical compound C1CC(O)CCN1C(=O)C1=CC=C(C=2[C@@H]([C@H](C3CCCC3)N(N=2)C=2C=C(Cl)C(C#N)=CC=2)CC2)C2=N1 UXHQLGLGLZKHTC-CUNXSJBXSA-N 0.000 description 1
- 102000016736 Cyclin Human genes 0.000 description 1
- 108050006400 Cyclin Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000208202 Linaceae Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000084978 Rena Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 102100029449 WD repeat-containing protein 61 Human genes 0.000 description 1
- 101710093149 WD repeat-containing protein 61 Proteins 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- -1 nucleoside triphosphates Chemical class 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 108700010839 phage proteins Proteins 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU884466372A SU1650698A1 (ru) | 1988-07-28 | 1988-07-28 | Фаговый вектор @ К9 дл конструировани геномных библиотек |
| PCT/SU1989/000204 WO1990001062A1 (fr) | 1988-07-28 | 1989-07-27 | PHAGE VECTEUR μ SK9 POUR LA FABRICATION DE GENOTHEQUES |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE9001070L SE9001070L (sv) | 1990-03-23 |
| SE9001070D0 SE9001070D0 (sv) | 1990-03-23 |
| SE465576B true SE465576B (sv) | 1991-09-30 |
Family
ID=21392243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE9001070A SE465576B (sv) | 1988-07-28 | 1990-03-23 | Fagvektor lambda sk9 framstaellning av genbanker |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPH03501331A (ja) |
| GB (1) | GB2228938A (ja) |
| SE (1) | SE465576B (ja) |
| SU (1) | SU1650698A1 (ja) |
| WO (1) | WO1990001062A1 (ja) |
-
1988
- 1988-07-28 SU SU884466372A patent/SU1650698A1/ru active
-
1989
- 1989-07-27 WO PCT/SU1989/000204 patent/WO1990001062A1/ru not_active Ceased
- 1989-07-27 JP JP1508623A patent/JPH03501331A/ja active Pending
-
1990
- 1990-03-22 GB GB9006475A patent/GB2228938A/en not_active Withdrawn
- 1990-03-23 SE SE9001070A patent/SE465576B/sv not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03501331A (ja) | 1991-03-28 |
| SE9001070L (sv) | 1990-03-23 |
| GB2228938A (en) | 1990-09-12 |
| SE9001070D0 (sv) | 1990-03-23 |
| SU1650698A1 (ru) | 1991-05-23 |
| WO1990001062A1 (fr) | 1990-02-08 |
| GB9006475D0 (en) | 1990-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Delaroque et al. | The complete DNA sequence of the Ectocarpus siliculosus virus EsV-1 genome | |
| Matthews et al. | The cloning of chromosomal DNA associated with methicillin and other resistances in Staphylococcus aureus | |
| Condit et al. | Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus | |
| Méchali et al. | Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeast | |
| Owen et al. | Characterization of cucumber mosaic virus I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains | |
| Neimark et al. | Isolation and characterization of full‐length chromosomes from non‐culturable plant‐pathogenic Mycoplasma‐like organisms | |
| Cai et al. | Construction and characterization of a bovine bacterial artificial chromosome library | |
| Liu et al. | Use of arbitrarily primed polymerase chain reaction to differentiate Trichophyton dermatophytes | |
| CN110183525B (zh) | 小麦抗条锈病相关的txr蛋白及其编码基因与应用 | |
| Ley et al. | Molecular cloning of African swine fever virus DNA | |
| EP0336412A2 (en) | Synthetic oligonucleotides useful for the determination of Chlamydia trachomatis in a biological sample | |
| Bainbridge | Genetics of microbes | |
| JPH04502107A (ja) | 挿入を有するかまたは欠失に相当するdnaの豊富化およびクローニングの方法 | |
| Abraham et al. | Cloning and analysis of the Clostridium perfringens tetracycline resistance plasmid, pCW3 | |
| Hong et al. | Construction of a BAC library and generation of BAC end sequence-tagged connectors for genome sequencing of the African malaria mosquito Anopheles gambiae | |
| JPH0527400B2 (ja) | ||
| Bloom et al. | Characterization of the Aleutian disease virus genome and its intracellular forms | |
| Grambow et al. | Deletion analysis of a bacteriophage P2 late promoter | |
| US6391631B1 (en) | Bacterial plasmids | |
| Samson et al. | The 5S ribosomal genes in the Drosophila melanogaster species subgroup. Nucleotide sequence of a 5S unit from Drosophila simulans and Drosophila teissieri | |
| CN108842000B (zh) | 用于鉴别DAdV-3和DAdV-A的引物组 | |
| US5863730A (en) | Procedure for the polymerization of nucleic acid sequences and its applications | |
| CN116555276A (zh) | 一种基因敲除选育fkbp10a基因缺失型斑马鱼的方法 | |
| Yin et al. | Dictyostelium giganteum plasmid Dgp1 is a member of the Ddp2 plasmid family | |
| JP2009514506A (ja) | E.コリ株dsm6601のプラスミド不含のクローン |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAV | Patent application has lapsed |
Ref document number: 9001070-3 Effective date: 19920622 Format of ref document f/p: F |