SK24094A3 - New plasmid for crm and difteric toxin production - Google Patents

New plasmid for crm and difteric toxin production Download PDF

Info

Publication number: SK24094A3
Authority: SK; Slovakia
Prior art keywords: plasmid; protein; gene; diphtheria toxin; crm197
Prior art date: 1993-03-05

Application number

SK240-94A

Other languages

English (en)

Slovak (sk)

Inventor

Benjamin J Metcalf

Original Assignee

American Cyanamid Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-03-05

Filing date

1994-02-28

Publication date

1995-03-08

1994-02-28 Application filed by American Cyanamid Co filed Critical American Cyanamid Co

1995-03-08 Publication of SK24094A3 publication Critical patent/SK24094A3/sk

Links

239000013612 plasmid Substances 0.000 title claims abstract description 45
238000004519 manufacturing process Methods 0.000 title claims description 17
239000003053 toxin Substances 0.000 title claims description 4
231100000765 toxin Toxicity 0.000 title claims description 4
108090000623 proteins and genes Proteins 0.000 claims abstract description 55
108010053187 Diphtheria Toxin Proteins 0.000 claims abstract description 39
102000016607 Diphtheria Toxin Human genes 0.000 claims abstract description 31
238000000034 method Methods 0.000 claims abstract description 18
244000005700 microbiome Species 0.000 claims abstract description 9
230000001131 transforming effect Effects 0.000 claims abstract description 3
102000004169 proteins and genes Human genes 0.000 claims description 29
241000186216 Corynebacterium Species 0.000 claims description 12
230000014509 gene expression Effects 0.000 claims description 12
238000010367 cloning Methods 0.000 claims description 11
230000010076 replication Effects 0.000 claims description 10
239000003550 marker Substances 0.000 claims description 7
206010013023 diphtheria Diseases 0.000 claims description 5
241000894006 Bacteria Species 0.000 claims description 2
238000004520 electroporation Methods 0.000 claims description 2
108090000765 processed proteins & peptides Proteins 0.000 claims description 2
102000004196 processed proteins & peptides Human genes 0.000 claims description 2
230000009466 transformation Effects 0.000 claims description 2
239000002773 nucleotide Substances 0.000 claims 1
125000003729 nucleotide group Chemical group 0.000 claims 1
241000894007 species Species 0.000 claims 1
108010071134 CRM197 (non-toxic variant of diphtheria toxin) Proteins 0.000 abstract description 25
241000186227 Corynebacterium diphtheriae Species 0.000 abstract description 19
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 9
101150044687 crm gene Proteins 0.000 abstract description 9
229910052742 iron Inorganic materials 0.000 abstract description 5
230000033228 biological regulation Effects 0.000 abstract description 2
239000013604 expression vector Substances 0.000 abstract 1
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WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 14
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101100298079 African swine fever virus (strain Badajoz 1971 Vero-adapted) pNG2 gene Proteins 0.000 description 2
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ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 2
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239000013598 vector Substances 0.000 description 2
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WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
BUCXEFZXWKUCCY-UHFFFAOYSA-N 4-methyl-3-(2-phenylethyl)-1,2,4-oxadiazol-5-one Chemical compound O1C(=O)N(C)C(CCC=2C=CC=CC=2)=N1 BUCXEFZXWKUCCY-UHFFFAOYSA-N 0.000 description 1
101100004408 Arabidopsis thaliana BIG gene Proteins 0.000 description 1
108010034055 CRM45 fragment of diphtheria toxin Proteins 0.000 description 1
101150042233 Chm gene Proteins 0.000 description 1
241000543381 Cliftonia monophylla Species 0.000 description 1
108020004705 Codon Proteins 0.000 description 1
108010060123 Conjugate Vaccines Proteins 0.000 description 1
241000186226 Corynebacterium glutamicum Species 0.000 description 1
241001337994 Cryptococcus <scale insect> Species 0.000 description 1
101001062350 Danio rerio Forkhead box protein A2 Proteins 0.000 description 1
101100485279 Drosophila melanogaster emb gene Proteins 0.000 description 1
102100029095 Exportin-1 Human genes 0.000 description 1
108700039691 Genetic Promoter Regions Proteins 0.000 description 1
108090000288 Glycoproteins Proteins 0.000 description 1
102000003886 Glycoproteins Human genes 0.000 description 1
241000606768 Haemophilus influenzae Species 0.000 description 1
241000588748 Klebsiella Species 0.000 description 1
101710085938 Matrix protein Proteins 0.000 description 1
101710127721 Membrane protein Proteins 0.000 description 1
206010028980 Neoplasm Diseases 0.000 description 1
241000208125 Nicotiana Species 0.000 description 1
235000002637 Nicotiana tabacum Nutrition 0.000 description 1
241001631646 Papillomaviridae Species 0.000 description 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
101100485284 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) CRM1 gene Proteins 0.000 description 1
241000607626 Vibrio cholerae Species 0.000 description 1
101150094313 XPO1 gene Proteins 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
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235000019365 chlortetracycline Nutrition 0.000 description 1
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238000010276 construction Methods 0.000 description 1
238000012258 culturing Methods 0.000 description 1
108010037782 cytomedins Proteins 0.000 description 1
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229940079593 drug Drugs 0.000 description 1
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229960003276 erythromycin Drugs 0.000 description 1
108700002148 exportin 1 Proteins 0.000 description 1
239000012634 fragment Substances 0.000 description 1
230000002538 fungal effect Effects 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
229940047650 haemophilus influenzae Drugs 0.000 description 1
229910001385 heavy metal Inorganic materials 0.000 description 1
208000006454 hepatitis Diseases 0.000 description 1
231100000283 hepatitis Toxicity 0.000 description 1
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238000003119 immunoblot Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
229960000318 kanamycin Drugs 0.000 description 1
229930027917 kanamycin Natural products 0.000 description 1
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
229930182823 kanamycin A Natural products 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
238000002703 mutagenesis Methods 0.000 description 1
231100000350 mutagenesis Toxicity 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
244000045947 parasite Species 0.000 description 1
238000002229 photoelectron microspectroscopy Methods 0.000 description 1
238000007747 plating Methods 0.000 description 1
235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
229920001184 polypeptide Polymers 0.000 description 1
229920000053 polysorbate 80 Polymers 0.000 description 1
239000000047 product Substances 0.000 description 1
238000010188 recombinant method Methods 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
201000005404 rubella Diseases 0.000 description 1
230000007017 scission Effects 0.000 description 1
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238000002741 site-directed mutagenesis Methods 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
239000002689 soil Substances 0.000 description 1
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230000000699 topical effect Effects 0.000 description 1
108700012359 toxins Proteins 0.000 description 1
238000002834 transmittance Methods 0.000 description 1
RYYVLZVUVIJVGH-UHFFFAOYSA-N trimethylxanthine Natural products CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 1
241000701161 unidentified adenovirus Species 0.000 description 1
229940118696 vibrio cholerae Drugs 0.000 description 1
108700026220 vif Genes Proteins 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biophysics (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

SK240-94A 1993-03-05 1994-02-28 New plasmid for crm and difteric toxin production SK24094A3 (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US2728393A	1993-03-05	1993-03-05

Publications (1)

Publication Number	Publication Date
SK24094A3 true SK24094A3 (en)	1995-03-08

Family

ID=21836767

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SK240-94A SK24094A3 (en)	1993-03-05	1994-02-28	New plasmid for crm and difteric toxin production

Country Status (21)

Country	Link
US (1)	US5614382A (de)
EP (1)	EP0616034B1 (de)
JP (1)	JP4144813B2 (de)
KR (1)	KR100316004B1 (de)
CN (1)	CN1100757A (de)
AT (1)	ATE280235T1 (de)
AU (1)	AU686126B2 (de)
BR (1)	BR1100634A (de)
CA (1)	CA2116914C (de)
CZ (1)	CZ39394A3 (de)
DE (1)	DE69434079T2 (de)
DK (1)	DK0616034T3 (de)
ES (1)	ES2231770T3 (de)
FI (1)	FI112090B (de)
HU (1)	HUT71320A (de)
IL (1)	IL108822A (de)
NO (1)	NO313758B1 (de)
NZ (1)	NZ260027A (de)
PT (1)	PT616034E (de)
SK (1)	SK24094A3 (de)
ZA (1)	ZA941548B (de)

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1994
- 1994-02-07 DK DK94101770T patent/DK0616034T3/da active
- 1994-02-07 DE DE69434079T patent/DE69434079T2/de not_active Expired - Lifetime
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Publication number	Publication date
DE69434079D1 (de)	2004-11-25
JPH06292593A (ja)	1994-10-21
FI112090B (fi)	2003-10-31
NO940774D0 (no)	1994-03-04
DE69434079T2 (de)	2005-02-24
US5614382A (en)	1997-03-25
CA2116914C (en)	2005-02-08
HUT71320A (en)	1995-11-28
CZ39394A3 (en)	1995-02-15
NZ260027A (en)	1996-03-26
KR100316004B1 (ko)	2002-02-19
EP0616034A2 (de)	1994-09-21
BR1100634A (pt)	1999-12-07
KR940021731A (ko)	1994-10-19
PT616034E (pt)	2005-02-28
FI941050A0 (fi)	1994-03-04
CA2116914A1 (en)	1994-09-06
NO940774L (no)	1994-09-06
CN1100757A (zh)	1995-03-29
EP0616034B1 (de)	2004-10-20
ZA941548B (en)	1994-10-03
NO313758B1 (no)	2002-11-25
ES2231770T3 (es)	2005-05-16
AU5759594A (en)	1994-09-08
IL108822A0 (en)	1994-06-24
DK0616034T3 (da)	2005-02-21
HU9400657D0 (en)	1994-05-30
IL108822A (en)	2004-09-27
JP4144813B2 (ja)	2008-09-03
ATE280235T1 (de)	2004-11-15
EP0616034A3 (en)	1996-10-16
FI941050L (fi)	1994-09-06
AU686126B2 (en)	1998-02-05

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JPH06506111A (ja)	1994-07-14	ボルデテラブロンキセプティカ外膜抗原
JPH02472A (ja)	1990-01-05	Ｌ―グルタミン酸の製造方法