SK280199B6 - Fragment dna(h) kódujúci polypeptid s nitril-hydra - Google Patents

Fragment dna(h) kódujúci polypeptid s nitril-hydra Download PDF

Info

Publication number: SK280199B6
Authority: SK; Slovakia
Prior art keywords: dna; nitrile hydratase; transformant; fragment; mixture
Prior art date: 1990-02-28

Application number

SK531-91A

Other languages

English (en)

Slovak (sk)

Inventor

Beppu Teruhiko

Yamada Hideaki

Nagasawa Toru

Horinouchi Sueharu

Nishiyama Nakoto

Original Assignee

Mitsubishi Rayon Co.

Beppu Teruhiko

Yamada Hideaki

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-02-28

Filing date

1991-02-28

Publication date

1999-09-10

1991-02-28 Application filed by Mitsubishi Rayon Co., Beppu Teruhiko, Yamada Hideaki filed Critical Mitsubishi Rayon Co.

1999-09-10 Publication of SK280199B6 publication Critical patent/SK280199B6/sk

Links

108010024026 Nitrile hydratase Proteins 0.000 title claims abstract description 56
239000012634 fragment Substances 0.000 title claims abstract description 52
238000000034 method Methods 0.000 title claims description 18
150000001408 amides Chemical class 0.000 title claims description 12
230000000694 effects Effects 0.000 title claims description 9
229920001184 polypeptide Polymers 0.000 title claims description 7
102000004196 processed proteins & peptides Human genes 0.000 title claims description 7
108090000765 processed proteins & peptides Proteins 0.000 title claims description 7
239000013598 vector Substances 0.000 claims abstract description 10
125000003275 alpha amino acid group Chemical group 0.000 claims abstract 3
108020004414 DNA Proteins 0.000 claims description 51
238000002360 preparation method Methods 0.000 claims description 12
108020004511 Recombinant DNA Proteins 0.000 claims description 10
230000001580 bacterial effect Effects 0.000 claims description 10
150000002825 nitriles Chemical class 0.000 claims description 10
108091028043 Nucleic acid sequence Proteins 0.000 claims description 7
239000002773 nucleotide Chemical group 0.000 abstract description 24
125000003729 nucleotide group Chemical group 0.000 abstract description 24
241000187693 Rhodococcus rhodochrous Species 0.000 abstract description 15
108090000623 proteins and genes Proteins 0.000 abstract description 6
244000005700 microbiome Species 0.000 abstract description 5
230000009466 transformation Effects 0.000 abstract description 5
239000013604 expression vector Substances 0.000 abstract description 2
239000000203 mixture Substances 0.000 description 37
239000013612 plasmid Substances 0.000 description 18
210000004027 cell Anatomy 0.000 description 14
150000001413 amino acids Chemical group 0.000 description 13
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
239000002609 medium Substances 0.000 description 12
239000000523 sample Substances 0.000 description 10
239000000725 suspension Substances 0.000 description 10
239000008188 pellet Substances 0.000 description 9
239000006228 supernatant Substances 0.000 description 9
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
239000013611 chromosomal DNA Substances 0.000 description 8
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 7
KXDAEFPNCMNJSK-UHFFFAOYSA-N Benzamide Chemical compound NC(=O)C1=CC=CC=C1 KXDAEFPNCMNJSK-UHFFFAOYSA-N 0.000 description 6
108090000790 Enzymes Proteins 0.000 description 6
102000004190 Enzymes Human genes 0.000 description 6
241000588724 Escherichia coli Species 0.000 description 6
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
241000187562 Rhodococcus sp. Species 0.000 description 6
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 6
229960000723 ampicillin Drugs 0.000 description 6
JFDZBHWFFUWGJE-UHFFFAOYSA-N benzonitrile Chemical compound N#CC1=CC=CC=C1 JFDZBHWFFUWGJE-UHFFFAOYSA-N 0.000 description 6
229940088598 enzyme Drugs 0.000 description 6
238000009396 hybridization Methods 0.000 description 6
-1 hydrates nitriles Chemical class 0.000 description 6
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
238000006243 chemical reaction Methods 0.000 description 5
238000002955 isolation Methods 0.000 description 5
239000011541 reaction mixture Substances 0.000 description 5
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
108020003215 DNA Probes Proteins 0.000 description 4
239000003298 DNA probe Substances 0.000 description 4
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 4
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 4
229960005542 ethidium bromide Drugs 0.000 description 4
238000000746 purification Methods 0.000 description 4
239000011780 sodium chloride Substances 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
108091036055 CccDNA Proteins 0.000 description 3
102000016943 Muramidase Human genes 0.000 description 3
108010014251 Muramidase Proteins 0.000 description 3
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 3
229940041514 candida albicans extract Drugs 0.000 description 3
210000000349 chromosome Anatomy 0.000 description 3
238000010276 construction Methods 0.000 description 3
239000000284 extract Substances 0.000 description 3
238000004128 high performance liquid chromatography Methods 0.000 description 3
229960000274 lysozyme Drugs 0.000 description 3
239000004325 lysozyme Substances 0.000 description 3
235000010335 lysozyme Nutrition 0.000 description 3
239000013600 plasmid vector Substances 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
238000012163 sequencing technique Methods 0.000 description 3
239000012138 yeast extract Substances 0.000 description 3
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
241000186216 Corynebacterium Species 0.000 description 2
238000001712 DNA sequencing Methods 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical class CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 2
241000187654 Nocardia Species 0.000 description 2
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
239000011543 agarose gel Substances 0.000 description 2
239000001110 calcium chloride Substances 0.000 description 2
229910001628 calcium chloride Inorganic materials 0.000 description 2
125000004432 carbon atom Chemical group C* 0.000 description 2
GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 2
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
235000019797 dipotassium phosphate Nutrition 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
238000009585 enzyme analysis Methods 0.000 description 2
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
229910052943 magnesium sulfate Inorganic materials 0.000 description 2
235000019341 magnesium sulphate Nutrition 0.000 description 2
229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
235000019796 monopotassium phosphate Nutrition 0.000 description 2
230000002572 peristaltic effect Effects 0.000 description 2
PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
239000011535 reaction buffer Substances 0.000 description 2
108091008146 restriction endonucleases Proteins 0.000 description 2
239000001632 sodium acetate Substances 0.000 description 2
235000017281 sodium acetate Nutrition 0.000 description 2
BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 2
229910001488 sodium perchlorate Inorganic materials 0.000 description 2
239000000243 solution Substances 0.000 description 2
BNBRIFIJRKJGEI-UHFFFAOYSA-N 2,6-difluorobenzonitrile Chemical compound FC1=CC=CC(F)=C1C#N BNBRIFIJRKJGEI-UHFFFAOYSA-N 0.000 description 1
FFNVQNRYTPFDDP-UHFFFAOYSA-N 2-cyanopyridine Chemical class N#CC1=CC=CC=N1 FFNVQNRYTPFDDP-UHFFFAOYSA-N 0.000 description 1
YXDXXGXWFJCXEB-UHFFFAOYSA-N 2-furonitrile Chemical compound N#CC1=CC=CO1 YXDXXGXWFJCXEB-UHFFFAOYSA-N 0.000 description 1
WSGYTJNNHPZFKR-UHFFFAOYSA-N 3-hydroxypropanenitrile Chemical compound OCCC#N WSGYTJNNHPZFKR-UHFFFAOYSA-N 0.000 description 1
GUPXYSSGJWIURR-UHFFFAOYSA-N 3-octoxypropane-1,2-diol Chemical compound CCCCCCCCOCC(O)CO GUPXYSSGJWIURR-UHFFFAOYSA-N 0.000 description 1
NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
241000186063 Arthrobacter Species 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
241000186146 Brevibacterium Species 0.000 description 1
102000012410 DNA Ligases Human genes 0.000 description 1
108010061982 DNA Ligases Proteins 0.000 description 1
241001524679 Escherichia virus M13 Species 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
GYCMBHHDWRMZGG-UHFFFAOYSA-N Methylacrylonitrile Chemical compound CC(=C)C#N GYCMBHHDWRMZGG-UHFFFAOYSA-N 0.000 description 1
241001467578 Microbacterium Species 0.000 description 1
241000192041 Micrococcus Species 0.000 description 1
108010033272 Nitrilase Proteins 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
239000008118 PEG 6000 Substances 0.000 description 1
239000001888 Peptone Substances 0.000 description 1
108010080698 Peptones Proteins 0.000 description 1
229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
241000316848 Rhodococcus <scale insect> Species 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
NKKMVIVFRUYPLQ-NSCUHMNNSA-N crotononitrile Chemical compound C\C=C\C#N NKKMVIVFRUYPLQ-NSCUHMNNSA-N 0.000 description 1
238000012258 culturing Methods 0.000 description 1
238000001514 detection method Methods 0.000 description 1
239000000385 dialysis solution Substances 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
239000000499 gel Substances 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000008103 glucose Substances 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
238000011534 incubation Methods 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
239000000463 material Substances 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
235000019319 peptone Nutrition 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
239000008057 potassium phosphate buffer Substances 0.000 description 1
238000011160 research Methods 0.000 description 1
238000012216 screening Methods 0.000 description 1
230000035939 shock Effects 0.000 description 1
238000000527 sonication Methods 0.000 description 1
238000009987 spinning Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
238000012360 testing method Methods 0.000 description 1
CUPOOAWTRIURFT-UHFFFAOYSA-N thiophene-2-carbonitrile Chemical compound N#CC1=CC=CS1 CUPOOAWTRIURFT-UHFFFAOYSA-N 0.000 description 1
239000012137 tryptone Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Chemical Kinetics & Catalysis (AREA)
Medicinal Chemistry (AREA)
General Chemical & Material Sciences (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)

SK531-91A 1990-02-28 1991-02-28 Fragment dna(h) kódujúci polypeptid s nitril-hydra SK280199B6 (sk)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
JP4807890		1990-02-28
US08/028,463 US5731176A (en)	1990-02-28	1993-03-09	DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant

Publications (1)

Publication Number	Publication Date
SK280199B6 true SK280199B6 (sk)	1999-09-10

Family

ID=26388301

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SK531-91A SK280199B6 (sk)	1990-02-28	1991-02-28	Fragment dna(h) kódujúci polypeptid s nitril-hydra

Country Status (18)

Country	Link
US (1)	US5731176A (da)
EP (1)	EP0445646B1 (da)
JP (1)	JP3162091B2 (da)
KR (1)	KR950002004B1 (da)
CN (1)	CN1054639C (da)
AT (1)	ATE138101T1 (da)
AU (1)	AU627648B2 (da)
CA (1)	CA2037291C (da)
CZ (2)	CZ284017B6 (da)
DE (1)	DE69119454T2 (da)
DK (1)	DK0445646T3 (da)
ES (1)	ES2089044T3 (da)
FI (1)	FI102539B (da)
MX (1)	MX193790B (da)
NO (1)	NO306684B1 (da)
PL (1)	PL166201B1 (da)
RU (1)	RU2081173C1 (da)
SK (1)	SK280199B6 (da)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2907479B2 (ja) *	1990-02-28	1999-06-21	輝彦別府	ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法
EP0502476B1 (en) *	1991-03-04	2001-07-18	Mitsubishi Rayon Co., Ltd.	Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids
AU3692593A (en) *	1992-04-28	1993-11-04	Mitsubishi Kasei Corporation	Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
JP3828590B2 (ja) *	1994-10-03	2006-10-04	昌清水	ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
JP3154633B2 (ja) *	1994-12-28	2001-04-09	三菱レイヨン株式会社	ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子
JPH11513255A (ja) *	1995-10-06	1999-11-16	イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー	立体特異的ニトリルヒドラターゼおよびアミダーゼ酵素をコードする核酸断片、ならびにキラルアミドおよび酸の生産に有用なそれら酵素を発現する組換え微生物
DE69739026D1 (de) *	1996-02-14	2008-11-20	Mitsui Chemicals Inc	Nitril-Hydratase, ihre Derivate und Verfahren zur Herstellung von Verbindungen
US5866379A (en) *	1997-01-28	1999-02-02	Novus International	Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
FR2770214B1 (fr) *	1997-10-24	1999-12-31	Rhodia Chimie Sa	Procede de preparation d'un benzamide halogene
JP2000050866A (ja) *	1998-08-06	2000-02-22	Rikagaku Kenkyusho	ニトリルヒドラターゼの生産方法
JP4185607B2 (ja)	1998-12-15	2008-11-26	ダイセル化学工業株式会社	新規な微生物及びアミド化合物の製造方法
RU2312145C2 (ru) *	1999-06-25	2007-12-10	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки, участвующие в синтезе мембран и мембранном транспорте
RU2304616C2 (ru) *	1999-06-25	2007-08-20	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки, участвующие в гомеостазе и адаптации
RU2303635C2 (ru) *	1999-06-25	2007-07-27	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки резистентности и толерантности к стрессам
RU2306339C2 (ru) *	1999-06-25	2007-09-20	Басф Акциенгезелльшафт	ГЕНЫ Corynebacterium glutamicum, КОДИРУЮЩИЕ БЕЛКИ МЕТАБОЛИЧЕСКИХ ПУТЕЙ
RU2310686C2 (ru) *	1999-06-25	2007-11-20	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки, участвующие в метаболизме углерода и продуцировании энергии
RU2326170C2 (ru) *	1999-07-01	2008-06-10	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки системы фосфоенолпируват-сахар-фосфотрансферазы
US7118898B1 (en)	1999-10-26	2006-10-10	Showa Denko K.K.	Rhodococcus bacterium, nitrilase gene, nitrylhydratase gene and amidase gene from Rhondococcus bacterium, and process for producing carboxylic acids by using them
DE10010149A1 (de) *	2000-03-03	2001-09-06	Basf Ag	Nitrilase aus Rhodococcus rhodochrous NCIMB 11216
US7749739B2 (en)	2000-12-20	2010-07-06	Dia-Nitrix Co., Ltd.	Process for producing amide compound using microbial catalyst
MX268424B (es) *	2001-01-09	2009-07-17	Lonza Ag	Procedimiento microbiologico para la produccion de amidas.
JP2002325587A (ja) *	2001-03-02	2002-11-12	Daicel Chem Ind Ltd	ニトリルヒドラターゼ、およびアミドの製造方法
JP2004194588A (ja)	2002-12-19	2004-07-15	Mitsui Chemicals Inc	新規なニトリルヒドラターゼ
WO2004067738A2 (en) *	2003-01-27	2004-08-12	Degussa Ag	Nitrile hydratases from rhodococcus erythropolis and their application
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1991
- 1991-02-22 AU AU71293/91A patent/AU627648B2/en not_active Expired
- 1991-02-27 MX MX9124722A patent/MX193790B/es unknown
- 1991-02-27 RU SU914894871A patent/RU2081173C1/ru active
- 1991-02-27 ES ES91102937T patent/ES2089044T3/es not_active Expired - Lifetime
- 1991-02-27 DE DE69119454T patent/DE69119454T2/de not_active Expired - Lifetime
- 1991-02-27 DK DK91102937.9T patent/DK0445646T3/da active
- 1991-02-27 AT AT91102937T patent/ATE138101T1/de not_active IP Right Cessation
- 1991-02-27 EP EP91102937A patent/EP0445646B1/en not_active Expired - Lifetime
- 1991-02-27 JP JP03323691A patent/JP3162091B2/ja not_active Expired - Lifetime
- 1991-02-27 FI FI910960A patent/FI102539B/fi active
- 1991-02-27 NO NO910797A patent/NO306684B1/no not_active IP Right Cessation
- 1991-02-28 CN CN91101323A patent/CN1054639C/zh not_active Expired - Lifetime
- 1991-02-28 CZ CZ943170A patent/CZ284017B6/cs not_active IP Right Cessation
- 1991-02-28 KR KR1019910003258A patent/KR950002004B1/ko not_active Expired - Lifetime
- 1991-02-28 CZ CS91531A patent/CZ284048B6/cs not_active IP Right Cessation
- 1991-02-28 PL PL91289239A patent/PL166201B1/pl unknown
- 1991-02-28 SK SK531-91A patent/SK280199B6/sk not_active IP Right Cessation
- 1991-02-28 CA CA002037291A patent/CA2037291C/en not_active Expired - Lifetime
1993
- 1993-03-09 US US08/028,463 patent/US5731176A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DK0445646T3 (da)	1996-09-30
DE69119454D1 (de)	1996-06-20
ES2089044T3 (es)	1996-10-01
US5731176A (en)	1998-03-24
CZ284048B6 (cs)	1998-07-15
FI910960L (fi)	1991-08-29
PL289239A1 (en)	1992-03-23
FI910960A0 (fi)	1991-02-27
JPH04211379A (ja)	1992-08-03
EP0445646B1 (en)	1996-05-15
MX193790B (en)	1999-10-19
DE69119454T2 (de)	1996-09-26
AU627648B2 (en)	1992-08-27
CN1054639C (zh)	2000-07-19
PL166201B1 (pl)	1995-04-28
AU7129391A (en)	1991-09-12
NO910797D0 (no)	1991-02-27
CZ317094A3 (cs)	1998-05-13
CZ284017B6 (cs)	1998-07-15
NO306684B1 (no)	1999-12-06
FI102539B1 (fi)	1998-12-31
EP0445646A3 (en)	1992-01-08
CS9100531A2 (en)	1991-10-15
NO910797L (no)	1991-08-29
KR950002004B1 (ko)	1995-03-08
RU2081173C1 (ru)	1997-06-10
JP3162091B2 (ja)	2001-04-25
CA2037291C (en)	2001-07-24
ATE138101T1 (de)	1996-06-15
FI102539B (fi)	1998-12-31
EP0445646A2 (en)	1991-09-11
KR910021473A (ko)	1991-12-20
CN1054616A (zh)	1991-09-18
CA2037291A1 (en)	1991-08-29

Legal Events

Date	Code	Title	Description
2011-04-05	MK4A	Patent expired	Expiry date: 20110228

Publication	Publication Date	Title
EP0445646B1 (en)	1996-05-15	DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the DNA fragment and a process for the production of amides using the transformant
US5888785A (en)	1999-03-30	Method for using hydratase or a hydratase-amidase fusion for stereospecifically bioconverting certain racemic nitriles to the corresponding enatiomeric R--or S-amide or s-carboxylic acid
US5130235A (en)	1992-07-14	Dna encoding polypeptide having nitrile hydratase activity and method for producing amides from nitriles with transformant containing the same
EP0444639B1 (en)	1996-01-31	A gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
JP3408737B2 (ja)	2003-05-19	ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子
US5648256A (en)	1997-07-15	Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
US5683913A (en)	1997-11-04	Regulatory gene for the expression of nitrile hydratase gene
US6617139B1 (en)	2003-09-09	Amidase gene
US5789211A (en)	1998-08-04	Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
US5753472A (en)	1998-05-19	DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
US5830693A (en)	1998-11-03	Gene encoding a regulatory factor involved in activating expression of the nitrilase gene promoter
EP0506262B1 (en)	1998-06-03	Production of L-fucose dehydrogenase
RU2173708C2 (ru)	2001-09-20	Фрагмент днк, кодирующий полипептид с активностью нитрилгидратазы, рекомбинантная плазмидная днк рnhj20l, кодирующая полипептид, штамм escherichia coli tg1/рnhj20l, способ получения полипептида со свойствами нитрилгидратазы
JP3091202B2 (ja)	2000-09-25	アセチルポリアミンアミドヒドロラーゼの製造法
JP3798460B2 (ja)	2006-07-19	コバルト輸送活性を有するポリペプチドおよびその遺伝子
JPH0568551A (ja)	1993-03-23	新規なｇｌ−７ａｃａアシラーゼ
JPH0716429B2 (ja)	1995-03-01	Ｌ−スレオニンの製造法