NO306684B1 - FremgangsmÕte for fremstilling av nitrilhydratase og anvendelse av nevnte nitrilhydratase og anvendelse av transformant inneholdende en rekombinant DNA som koder for et polypeptid med nitrilhydrataseaktivitet - Google Patents

FremgangsmÕte for fremstilling av nitrilhydratase og anvendelse av nevnte nitrilhydratase og anvendelse av transformant inneholdende en rekombinant DNA som koder for et polypeptid med nitrilhydrataseaktivitet Download PDF

Info

Publication number: NO306684B1
Authority: NO; Norway
Prior art keywords: nitrile hydratase; dna; transformant; subunit; added
Prior art date: 1990-02-28

Application number

NO910797A

Other languages

English (en)

Norwegian (no)

Other versions

NO910797L (no

NO910797D0 (no

Inventor

Teruhiko Beppu

Hideaki Yamada

Toru Nagasawa

Sueharu Horinouchi

Makoto Nishiyama

Original Assignee

Mitsubishi Rayon Co

Teruhiko Beppu

Hideaki Yamada

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-02-28

Filing date

1991-02-27

Publication date

1999-12-06

1991-02-27 Application filed by Mitsubishi Rayon Co, Teruhiko Beppu, Hideaki Yamada filed Critical Mitsubishi Rayon Co

1991-02-27 Publication of NO910797D0 publication Critical patent/NO910797D0/no

1991-08-29 Publication of NO910797L publication Critical patent/NO910797L/no

1999-12-06 Publication of NO306684B1 publication Critical patent/NO306684B1/no

Links

108010024026 Nitrile hydratase Proteins 0.000 title claims abstract description 59
229920001184 polypeptide Polymers 0.000 title claims abstract description 13
102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 13
108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 13
230000000694 effects Effects 0.000 title claims abstract description 11
108020004511 Recombinant DNA Proteins 0.000 title claims abstract description 8
238000000034 method Methods 0.000 title claims description 14
238000002360 preparation method Methods 0.000 title claims description 5
108020004414 DNA Proteins 0.000 claims abstract description 49
239000012634 fragment Substances 0.000 claims abstract description 43
150000001408 amides Chemical class 0.000 claims abstract description 13
150000002825 nitriles Chemical class 0.000 claims abstract description 13
230000001580 bacterial effect Effects 0.000 claims abstract description 11
239000000463 material Substances 0.000 claims abstract description 6
239000013598 vector Substances 0.000 claims description 11
239000002773 nucleotide Substances 0.000 claims description 9
125000003729 nucleotide group Chemical group 0.000 claims description 9
238000004519 manufacturing process Methods 0.000 claims description 8
230000036571 hydration Effects 0.000 claims description 4
238000006703 hydration reaction Methods 0.000 claims description 4
238000011084 recovery Methods 0.000 claims description 2
102000004190 Enzymes Human genes 0.000 abstract description 9
108090000790 Enzymes Proteins 0.000 abstract description 9
108091028043 Nucleic acid sequence Proteins 0.000 abstract description 4
238000012258 culturing Methods 0.000 abstract 2
230000000887 hydrating effect Effects 0.000 abstract 2
239000000203 mixture Substances 0.000 description 34
239000013612 plasmid Substances 0.000 description 17
210000004027 cell Anatomy 0.000 description 14
241000187693 Rhodococcus rhodochrous Species 0.000 description 13
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
150000001413 amino acids Chemical class 0.000 description 11
239000000523 sample Substances 0.000 description 9
239000006228 supernatant Substances 0.000 description 9
239000000725 suspension Substances 0.000 description 9
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
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KXDAEFPNCMNJSK-UHFFFAOYSA-N Benzamide Chemical compound NC(=O)C1=CC=CC=C1 KXDAEFPNCMNJSK-UHFFFAOYSA-N 0.000 description 6
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AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 6
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QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
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238000004587 chromatography analysis Methods 0.000 description 4
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 4
229960005542 ethidium bromide Drugs 0.000 description 4
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238000002955 isolation Methods 0.000 description 4
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238000000746 purification Methods 0.000 description 4
239000011541 reaction mixture Substances 0.000 description 4
239000011780 sodium chloride Substances 0.000 description 4
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125000004432 carbon atom Chemical group C* 0.000 description 2
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BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
238000013507 mapping Methods 0.000 description 2
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235000019796 monopotassium phosphate Nutrition 0.000 description 2
230000002572 peristaltic effect Effects 0.000 description 2
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
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229920006395 saturated elastomer Polymers 0.000 description 2
239000001632 sodium acetate Substances 0.000 description 2
235000017281 sodium acetate Nutrition 0.000 description 2
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
230000009466 transformation Effects 0.000 description 2
230000001131 transforming effect Effects 0.000 description 2
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GUPXYSSGJWIURR-UHFFFAOYSA-N 3-octoxypropane-1,2-diol Chemical compound CCCCCCCCOCC(O)CO GUPXYSSGJWIURR-UHFFFAOYSA-N 0.000 description 1
NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
241000186063 Arthrobacter Species 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
241000186146 Brevibacterium Species 0.000 description 1
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108010061982 DNA Ligases Proteins 0.000 description 1
241001524679 Escherichia virus M13 Species 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
GYCMBHHDWRMZGG-UHFFFAOYSA-N Methylacrylonitrile Chemical compound CC(=C)C#N GYCMBHHDWRMZGG-UHFFFAOYSA-N 0.000 description 1
241001467578 Microbacterium Species 0.000 description 1
241000192041 Micrococcus Species 0.000 description 1
108010033272 Nitrilase Proteins 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
241000187654 Nocardia Species 0.000 description 1
108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
239000001888 Peptone Substances 0.000 description 1
108010080698 Peptones Proteins 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
241000316848 Rhodococcus <scale insect> Species 0.000 description 1
108010046983 Ribonuclease T1 Proteins 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
239000008272 agar Substances 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
239000001166 ammonium sulphate Substances 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
NKKMVIVFRUYPLQ-NSCUHMNNSA-N crotononitrile Chemical compound C\C=C\C#N NKKMVIVFRUYPLQ-NSCUHMNNSA-N 0.000 description 1
PMSVVUSIPKHUMT-UHFFFAOYSA-N cyanopyrazine Chemical compound N#CC1=CN=CC=N1 PMSVVUSIPKHUMT-UHFFFAOYSA-N 0.000 description 1
238000001514 detection method Methods 0.000 description 1
239000000385 dialysis solution Substances 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
239000013604 expression vector Substances 0.000 description 1
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239000008103 glucose Substances 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
235000019319 peptone Nutrition 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
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CUPOOAWTRIURFT-UHFFFAOYSA-N thiophene-2-carbonitrile Chemical compound N#CC1=CC=CS1 CUPOOAWTRIURFT-UHFFFAOYSA-N 0.000 description 1
239000012137 tryptone Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Chemical Kinetics & Catalysis (AREA)
Medicinal Chemistry (AREA)
General Chemical & Material Sciences (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)

NO910797A 1990-02-28 1991-02-27 FremgangsmÕte for fremstilling av nitrilhydratase og anvendelse av nevnte nitrilhydratase og anvendelse av transformant inneholdende en rekombinant DNA som koder for et polypeptid med nitrilhydrataseaktivitet NO306684B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
JP4807890		1990-02-28
US08/028,463 US5731176A (en)	1990-02-28	1993-03-09	DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant

Publications (3)

Publication Number	Publication Date
NO910797D0 NO910797D0 (no)	1991-02-27
NO910797L NO910797L (no)	1991-08-29
NO306684B1 true NO306684B1 (no)	1999-12-06

Family

ID=26388301

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO910797A NO306684B1 (no)	1990-02-28	1991-02-27	FremgangsmÕte for fremstilling av nitrilhydratase og anvendelse av nevnte nitrilhydratase og anvendelse av transformant inneholdende en rekombinant DNA som koder for et polypeptid med nitrilhydrataseaktivitet

Country Status (18)

Country	Link
US (1)	US5731176A (da)
EP (1)	EP0445646B1 (da)
JP (1)	JP3162091B2 (da)
KR (1)	KR950002004B1 (da)
CN (1)	CN1054639C (da)
AT (1)	ATE138101T1 (da)
AU (1)	AU627648B2 (da)
CA (1)	CA2037291C (da)
CZ (2)	CZ284017B6 (da)
DE (1)	DE69119454T2 (da)
DK (1)	DK0445646T3 (da)
ES (1)	ES2089044T3 (da)
FI (1)	FI102539B1 (da)
MX (1)	MX193790B (da)
NO (1)	NO306684B1 (da)
PL (1)	PL166201B1 (da)
RU (1)	RU2081173C1 (da)
SK (1)	SK280199B6 (da)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2907479B2 (ja) *	1990-02-28	1999-06-21	輝彦別府	ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法
DE69231939T2 (de) *	1991-03-04	2002-04-04	Teruhiko Beppu	Hybrid-Plasmid-Vektoren, die für Nitril-abbauende Enzyme kodierende Gene enthalten und Verfahren zur Herstellung von Amiden und Säuren
AU3692593A (en) *	1992-04-28	1993-11-04	Mitsubishi Kasei Corporation	Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
JP3828590B2 (ja) *	1994-10-03	2006-10-04	昌清水	ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
JP3154633B2 (ja) *	1994-12-28	2001-04-09	三菱レイヨン株式会社	ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子
JPH11513255A (ja) *	1995-10-06	1999-11-16	イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー	立体特異的ニトリルヒドラターゼおよびアミダーゼ酵素をコードする核酸断片、ならびにキラルアミドおよび酸の生産に有用なそれら酵素を発現する組換え微生物
CN1240833C (zh) *	1996-02-14	2006-02-08	三井化学株式会社	新型腈水合酶
US5866379A (en) *	1997-01-28	1999-02-02	Novus International	Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
FR2770214B1 (fr) *	1997-10-24	1999-12-31	Rhodia Chimie Sa	Procede de preparation d'un benzamide halogene
JP2000050866A (ja) *	1998-08-06	2000-02-22	Rikagaku Kenkyusho	ニトリルヒドラターゼの生産方法
JP4185607B2 (ja)	1998-12-15	2008-11-26	ダイセル化学工業株式会社	新規な微生物及びアミド化合物の製造方法
RU2310686C2 (ru) *	1999-06-25	2007-11-20	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки, участвующие в метаболизме углерода и продуцировании энергии
RU2304616C2 (ru) *	1999-06-25	2007-08-20	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки, участвующие в гомеостазе и адаптации
RU2312145C2 (ru) *	1999-06-25	2007-12-10	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки, участвующие в синтезе мембран и мембранном транспорте
RU2306339C2 (ru) *	1999-06-25	2007-09-20	Басф Акциенгезелльшафт	ГЕНЫ Corynebacterium glutamicum, КОДИРУЮЩИЕ БЕЛКИ МЕТАБОЛИЧЕСКИХ ПУТЕЙ
RU2303635C2 (ru) *	1999-06-25	2007-07-27	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки резистентности и толерантности к стрессам
RU2326170C2 (ru) *	1999-07-01	2008-06-10	Басф Акциенгезелльшафт	Гены corynebacterium glutamicum, кодирующие белки системы фосфоенолпируват-сахар-фосфотрансферазы
CN1340101A (zh)	1999-10-26	2002-03-13	昭和电工株式会社	新型红球菌属细菌、源自于红球菌属细菌的腈水解酶基因、腈水合酶基因和酰胺酶基因,以及利用它们生产羧酸的方法
DE10010149A1 (de) *	2000-03-03	2001-09-06	Basf Ag	Nitrilase aus Rhodococcus rhodochrous NCIMB 11216
RU2288270C2 (ru) *	2000-12-20	2006-11-27	Диа-Нитрикс Ко., Лтд.	Способ производства амидного соединения с применением микробного катализатора
CZ20031409A3 (cs) *	2001-01-09	2003-11-12	Lonza Ag	Mikroorganismy a způsob výroby amidů
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1991
- 1991-02-22 AU AU71293/91A patent/AU627648B2/en not_active Expired
- 1991-02-27 NO NO910797A patent/NO306684B1/no not_active IP Right Cessation
- 1991-02-27 EP EP91102937A patent/EP0445646B1/en not_active Expired - Lifetime
- 1991-02-27 DE DE69119454T patent/DE69119454T2/de not_active Expired - Lifetime
- 1991-02-27 FI FI910960A patent/FI102539B1/fi active
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- 1991-02-27 DK DK91102937.9T patent/DK0445646T3/da active
- 1991-02-27 JP JP03323691A patent/JP3162091B2/ja not_active Expired - Lifetime
- 1991-02-27 ES ES91102937T patent/ES2089044T3/es not_active Expired - Lifetime
- 1991-02-27 RU SU914894871A patent/RU2081173C1/ru active
- 1991-02-27 MX MX9124722A patent/MX193790B/es unknown
- 1991-02-28 CN CN91101323A patent/CN1054639C/zh not_active Expired - Lifetime
- 1991-02-28 CA CA002037291A patent/CA2037291C/en not_active Expired - Lifetime
- 1991-02-28 KR KR1019910003258A patent/KR950002004B1/ko not_active Expired - Lifetime
- 1991-02-28 SK SK531-91A patent/SK280199B6/sk not_active IP Right Cessation
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1993
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Also Published As

Publication number	Publication date
JPH04211379A (ja)	1992-08-03
CZ317094A3 (cs)	1998-05-13
NO910797L (no)	1991-08-29
NO910797D0 (no)	1991-02-27
EP0445646A3 (en)	1992-01-08
CA2037291C (en)	2001-07-24
PL166201B1 (pl)	1995-04-28
PL289239A1 (en)	1992-03-23
CA2037291A1 (en)	1991-08-29
ATE138101T1 (de)	1996-06-15
AU7129391A (en)	1991-09-12
ES2089044T3 (es)	1996-10-01
FI102539B (fi)	1998-12-31
CZ284017B6 (cs)	1998-07-15
KR950002004B1 (ko)	1995-03-08
CN1054616A (zh)	1991-09-18
DE69119454D1 (de)	1996-06-20
CZ284048B6 (cs)	1998-07-15
DE69119454T2 (de)	1996-09-26
SK280199B6 (sk)	1999-09-10
CN1054639C (zh)	2000-07-19
MX193790B (en)	1999-10-19
FI910960L (fi)	1991-08-29
EP0445646A2 (en)	1991-09-11
EP0445646B1 (en)	1996-05-15
CS9100531A2 (en)	1991-10-15
AU627648B2 (en)	1992-08-27
JP3162091B2 (ja)	2001-04-25
US5731176A (en)	1998-03-24
KR910021473A (ko)	1991-12-20
RU2081173C1 (ru)	1997-06-10
FI102539B1 (fi)	1998-12-31
DK0445646T3 (da)	1996-09-30
FI910960A0 (fi)	1991-02-27

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2011-03-07	MK1K	Patent expired

Publication	Publication Date	Title
NO306684B1 (no)	1999-12-06	FremgangsmÕte for fremstilling av nitrilhydratase og anvendelse av nevnte nitrilhydratase og anvendelse av transformant inneholdende en rekombinant DNA som koder for et polypeptid med nitrilhydrataseaktivitet
US6251650B1 (en)	2001-06-26	Pseudomonas putida amidase polypeptide useful for the production of chiral amides and acids
JP2840253B2 (ja)	1998-12-24	ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法
EP0444639B1 (en)	1996-01-31	A gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
JP3380133B2 (ja)	2003-02-24	新規なニトリルヒドラターゼ
CA2090571A1 (en)	1993-08-28	Cephalosporin c acylase
US7074606B2 (en)	2006-07-11	Process for the preparation of (S) - or (R) -3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid
US5648256A (en)	1997-07-15	Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
US5314819A (en)	1994-05-24	Protein having nitrile hydratase activity obtained from rhizobium, gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
US5753472A (en)	1998-05-19	DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
JP3828590B2 (ja)	2006-10-04	ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
US5789211A (en)	1998-08-04	Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
JP4287144B2 (ja)	2009-07-01	新規ギ酸脱水素酵素及びその製造方法
WO2000063354A1 (en)	2000-10-26	Novel amidase gene
EP0726958A1 (en)	1996-08-21	A new cephalosporin c acylase
JP3709422B2 (ja)	2005-10-26	ニトリラーゼ遺伝子発現に関わる調節因子およびその遺伝子
RU2173708C2 (ru)	2001-09-20	Фрагмент днк, кодирующий полипептид с активностью нитрилгидратазы, рекомбинантная плазмидная днк рnhj20l, кодирующая полипептид, штамм escherichia coli tg1/рnhj20l, способ получения полипептида со свойствами нитрилгидратазы
JP2001292772A (ja)	2001-10-23	ロドコッカス属細菌由来のニトリルヒドラターゼ遺伝子およびアミダーゼ遺伝子
JP3798460B2 (ja)	2006-07-19	コバルト輸送活性を有するポリペプチドおよびその遺伝子
CA2024145A1 (en)	1991-03-05	Expression plasmids and use thereof
KR20170099510A (ko)	2017-09-01	박테로이데스 속 미생물 유래의 ｇａｂａ 생성 고활성 글루탐산탈탄산효소 및 이의 용도
AU6033496A (en)	1996-12-30	Chondroitinase production in recombinant proteus vulgaris st rains
JP2005151999A (ja)	2005-06-16	ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
JPH0568551A (ja)	1993-03-23	新規なｇｌ−７ａｃａアシラーゼ
JPWO2001025443A1 (ja)	2003-04-22	マンノースイソメラーゼ遺伝子