SK5792003A3 - Methods for large scale production of recombinant DNA-derived tPA or K2S molecules - Google Patents

Methods for large scale production of recombinant DNA-derived tPA or K2S molecules Download PDF

Info

Publication number: SK5792003A3
Authority: SK; Slovakia
Prior art keywords: dna; leu; ser; gly; variant
Prior art date: 2000-11-14

Application number

SK579-2003A

Other languages

English (en)

Slovak (sk)

Inventor

Rolf-Gunther Werner

Friedrich Goetz

Chatchai Tayapiwatana

Jiradej Manosroi

Aranya Manosroi

Original Assignee

Boehringer Ingelheim Int

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-11-14

Filing date

2001-11-07

Publication date

2004-01-08

2001-11-07 Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int

2004-01-08 Publication of SK5792003A3 publication Critical patent/SK5792003A3/sk

Links

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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C—CHEMISTRY; METALLURGY
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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Preparation Of Compounds By Using Micro-Organisms (AREA)
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Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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SK579-2003A 2000-11-14 2001-11-07 Methods for large scale production of recombinant DNA-derived tPA or K2S molecules SK5792003A3 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GBGB0027779.8A GB0027779D0 (en)	2000-11-14	2000-11-14	Methods for large scale production of recombinant dna-derived tpa or k2s molecules
PCT/EP2001/012857 WO2002040650A2 (en)	2000-11-14	2001-11-07	Methods for large scale production of recombinant dna-derived tpa or k2s molecules

Publications (1)

Publication Number	Publication Date
SK5792003A3 true SK5792003A3 (en)	2004-01-08

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Application Number	Title	Priority Date	Filing Date
SK579-2003A SK5792003A3 (en)	2000-11-14	2001-11-07	Methods for large scale production of recombinant DNA-derived tPA or K2S molecules

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EP (1)	EP1407030B1 (cs)
JP (1)	JP2004520018A (cs)
KR (1)	KR100856346B1 (cs)
CN (1)	CN100567495C (cs)
AR (1)	AR031334A1 (cs)
AT (1)	ATE504652T1 (cs)
AU (2)	AU2002221815B2 (cs)
BG (1)	BG66121B1 (cs)
BR (1)	BR0115344A (cs)
CA (1)	CA2428642C (cs)
CZ (1)	CZ20031657A3 (cs)
DE (1)	DE60144395D1 (cs)
EA (1)	EA005163B1 (cs)
EC (1)	ECSP034573A (cs)
EE (1)	EE200300232A (cs)
GB (1)	GB0027779D0 (cs)
HR (1)	HRP20030377A2 (cs)
HU (1)	HUP0301619A3 (cs)
IL (2)	IL155568A0 (cs)
MX (1)	MXPA03004161A (cs)
MY (1)	MY129838A (cs)
NO (1)	NO20032143L (cs)
NZ (1)	NZ526280A (cs)
PL (1)	PL206783B1 (cs)
SK (1)	SK5792003A3 (cs)
TW (1)	TWI292782B (cs)
UA (1)	UA75102C2 (cs)
UY (1)	UY27020A1 (cs)
WO (1)	WO2002040650A2 (cs)
YU (1)	YU36103A (cs)
ZA (1)	ZA200303547B (cs)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6955910B2 (en)	2000-11-14	2005-10-18	Boehringer Ingelheim International Gmbh	Method for large scale production of recombinant DNA-derived TPA or K2S molecules
US7087412B2 (en)	2000-11-14	2006-08-08	Boehringer Ingelheim International Gmbh	Methods for large scale protein production in prokaryotes
IL190184A0 (en) *	2008-03-16	2009-02-11	Hadasit Med Res Service	tPA MUTANT IN THE TREATMENT OF ACUTE BRAIN INJURY AND NEURODEGENERATIVE DISORDERS

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL87276A (en) *	1987-08-03	1995-07-31	Fujisawa Pharmaceutical Co	Analog of tissue plasminogen activator comprising only the kringle 2 and protease domain dna encoding the same processes for the preparation thereof and pharmaceutical compositions containing the same
AU2660397A (en) *	1996-04-05	1997-10-29	Board Of Regents, The University Of Texas System	Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
US20020061576A1 (en) *	1997-05-28	2002-05-23	Paul A. Moore	Tissue plasminogen activator-like protease
US6083715A (en) *	1997-06-09	2000-07-04	Board Of Regents, The University Of Texas System	Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
EP1048732A1 (de) *	1999-04-26	2000-11-02	F. Hoffmann-La Roche Ag	Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
EP1077263A1 (de) *	1999-07-29	2001-02-21	F.Hoffmann-La Roche Ag	Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
GB0027782D0 (en) *	2000-11-14	2000-12-27	Boehringer Ingelheim Int	Methods fro large scale protein productio in prokaryotes

2000
- 2000-11-14 GB GBGB0027779.8A patent/GB0027779D0/en not_active Ceased
2001
- 2001-07-11 UA UA2003065487A patent/UA75102C2/uk unknown
- 2001-11-07 JP JP2002543647A patent/JP2004520018A/ja active Pending
- 2001-11-07 YU YU36103A patent/YU36103A/sh unknown
- 2001-11-07 EA EA200300502A patent/EA005163B1/ru not_active IP Right Cessation
- 2001-11-07 KR KR1020037006567A patent/KR100856346B1/ko not_active Expired - Fee Related
- 2001-11-07 DE DE60144395T patent/DE60144395D1/de not_active Expired - Lifetime
- 2001-11-07 MX MXPA03004161A patent/MXPA03004161A/es active IP Right Grant
- 2001-11-07 CN CNB018188982A patent/CN100567495C/zh not_active Expired - Fee Related
- 2001-11-07 WO PCT/EP2001/012857 patent/WO2002040650A2/en not_active Ceased
- 2001-11-07 HR HR20030377A patent/HRP20030377A2/hr not_active Application Discontinuation
- 2001-11-07 EP EP01996596A patent/EP1407030B1/en not_active Expired - Lifetime
- 2001-11-07 CZ CZ20031657A patent/CZ20031657A3/cs unknown
- 2001-11-07 PL PL361881A patent/PL206783B1/pl not_active IP Right Cessation
- 2001-11-07 AU AU2002221815A patent/AU2002221815B2/en not_active Ceased
- 2001-11-07 NZ NZ526280A patent/NZ526280A/en unknown
- 2001-11-07 BR BR0115344-7A patent/BR0115344A/pt not_active IP Right Cessation
- 2001-11-07 CA CA2428642A patent/CA2428642C/en not_active Expired - Fee Related
- 2001-11-07 HU HU0301619A patent/HUP0301619A3/hu unknown
- 2001-11-07 AU AU2181502A patent/AU2181502A/xx active Pending
- 2001-11-07 AT AT01996596T patent/ATE504652T1/de not_active IP Right Cessation
- 2001-11-07 EE EEP200300232A patent/EE200300232A/xx unknown
- 2001-11-07 IL IL15556801A patent/IL155568A0/xx unknown
- 2001-11-07 SK SK579-2003A patent/SK5792003A3/sk unknown
- 2001-11-12 MY MYPI20015201A patent/MY129838A/en unknown
- 2001-11-12 UY UY27020A patent/UY27020A1/es not_active Application Discontinuation
- 2001-11-13 TW TW090128128A patent/TWI292782B/zh not_active IP Right Cessation
- 2001-11-14 AR ARP010105298A patent/AR031334A1/es unknown
2003
- 2003-04-24 IL IL155568A patent/IL155568A/en not_active IP Right Cessation
- 2003-04-29 EC EC2003004573A patent/ECSP034573A/es unknown
- 2003-05-08 ZA ZA200303547A patent/ZA200303547B/en unknown
- 2003-05-08 BG BG107780A patent/BG66121B1/bg unknown
- 2003-05-13 NO NO20032143A patent/NO20032143L/no not_active Application Discontinuation

Also Published As

Publication number	Publication date
CN100567495C (zh)	2009-12-09
AU2181502A (en)	2002-05-27
BG66121B1 (bg)	2011-05-31
UA75102C2 (en)	2006-03-15
TWI292782B (en)	2008-01-21
IL155568A0 (en)	2003-11-23
HUP0301619A3 (en)	2006-11-28
AU2002221815B2 (en)	2007-08-23
MXPA03004161A (es)	2004-04-20
NO20032143D0 (no)	2003-05-13
WO2002040650A2 (en)	2002-05-23
MY129838A (en)	2007-05-31
NO20032143L (no)	2003-07-07
CA2428642C (en)	2010-03-30
DE60144395D1 (de)	2011-05-19
PL206783B1 (pl)	2010-09-30
CZ20031657A3 (cs)	2003-10-15
KR20030059252A (ko)	2003-07-07
NZ526280A (en)	2006-02-24
BR0115344A (pt)	2003-08-26
AR031334A1 (es)	2003-09-17
IL155568A (en)	2010-02-17
GB0027779D0 (en)	2000-12-27
HUP0301619A2 (hu)	2003-09-29
EE200300232A (et)	2003-10-15
PL361881A1 (en)	2004-10-04
ZA200303547B (en)	2004-05-10
EA200300502A1 (ru)	2003-12-25
CA2428642A1 (en)	2002-05-23
EP1407030B1 (en)	2011-04-06
KR100856346B1 (ko)	2008-09-04
HRP20030377A2 (en)	2005-04-30
ECSP034573A (es)	2003-05-26
EA005163B1 (ru)	2004-12-30
BG107780A (bg)	2004-02-27
YU36103A (sh)	2006-05-25
WO2002040650A3 (en)	2003-12-31
ATE504652T1 (de)	2011-04-15
EP1407030A2 (en)	2004-04-14
UY27020A1 (es)	2002-07-31
HK1070097A1 (zh)	2005-06-10
CN1541271A (zh)	2004-10-27
JP2004520018A (ja)	2004-07-08

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2012-02-03	FB9A	Suspension of patent application procedure

Publication	Publication Date	Title
KR100188302B1 (ko)	1999-06-01	활성화피브린용해성과항-혈전성단백질
JP5539896B2 (ja)	2014-07-02	組換え的に改変されたプラスミン
JPH0698002B2 (ja)	1994-12-07	ヒトプロティンｃ活性の発現ベクター
ES2332124T3 (es)	2010-01-27	Metodo para la produccion a gran escala del dominio kringle 2 mas serina proteasa (k2s) de plasminogeno en procariotas.
US7087412B2 (en)	2006-08-08	Methods for large scale protein production in prokaryotes
US6955910B2 (en)	2005-10-18	Method for large scale production of recombinant DNA-derived TPA or K2S molecules
SK5792003A3 (en)	2004-01-08	Methods for large scale production of recombinant DNA-derived tPA or K2S molecules
US20100015123A1 (en)	2010-01-21	Novel thrombolytic molecules and a process therefor
Chiron et al.	1996	Pseudomonas exotoxin exhibits increased sensitivity to furin when sequences at the cleavage site are mutated to resemble the arginine‐rich loop of diphtheria toxin
AU2002221815A1 (en)	2002-08-01	Methods for large scale production of recombinant DNA-derived tPA or K2S molecules
JP3061199B2 (ja)	2000-07-10	エリトリナ・カッフラ型インヒビターの突然変異体及びセリンプロテアーゼを精製するための前記突然変異体の利用
HK1070097B (en)	2010-02-19	Methods for large scale production of recombinant dna-derived tpa or k2s molecules
JP2846961B2 (ja)	1999-01-13	エリトリナ・カフラ由来の組換え阻害物質のセリンプロテアーゼ精製への使用
HK1077848B (en)	2009-06-26	Methods for large scale protein production in prokaryotes