TWI391489B - 處理纖維素材料之方法及用於彼之酶類 - Google Patents

處理纖維素材料之方法及用於彼之酶類 Download PDF

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Publication number: TWI391489B
Authority: TW; Taiwan
Prior art keywords: enzyme; bcrc; thermophilum; cbh; acremonium
Prior art date: 2005-12-22

Application number

TW095148542A

Other languages

English (en)

Chinese (zh)

Other versions

TW200801195A (en

Inventor

Jari Vehmaanpera

Marika Alapuranen

Terhi Puranen

Matti Siika-Aho

Jarno Kallio

Satu Hooman

Sanni Voutilainen

Teemu Halonen

Liisa Viikari

Original Assignee

Roal Oy

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2005-12-22

Filing date

2006-12-22

Publication date

2013-04-01

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38188306&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=TWI391489(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

2006-12-22 Application filed by Roal Oy filed Critical Roal Oy

2008-01-01 Publication of TW200801195A publication Critical patent/TW200801195A/zh

2013-04-01 Application granted granted Critical

2013-04-01 Publication of TWI391489B publication Critical patent/TWI391489B/zh

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Classifications

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- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
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- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
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- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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TW095148542A 2005-12-22 2006-12-22 處理纖維素材料之方法及用於彼之酶類 TWI391489B (zh)

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AR (1)	AR058721A1 (pt)
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Families Citing this family (206)

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Publication number	Priority date	Publication date	Assignee	Title
EP1984514A4 (en) *	2006-01-27	2010-09-01	Univ Massachusetts	SYSTEMS AND METHOD FOR PRODUCING BIOFUELS AND CORRESPONDING MATERIALS
NZ614096A (en)	2006-10-26	2014-06-27	Xyleco Inc	Processing biomass
DE102006062045A1 (de)	2006-12-29	2008-07-03	Ab Enzymes Gmbh	Verbessertes Verfahren zur Gewinnung von Öl aus Pflanzensamen
US8546126B2 (en) *	2007-03-21	2013-10-01	Danisco Us Inc.	Over expression of foldases and chaperones improves protein production
US7923236B2 (en) *	2007-08-02	2011-04-12	Dyadic International (Usa), Inc.	Fungal enzymes
US7851193B2 (en)	2007-12-05	2010-12-14	Novozymes A/S	Polypeptides having xylanase activity and polynucleotides encoding same
AU2008343105A1 (en)	2007-12-19	2009-07-09	Novozymes A/S	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
AU2009219150A1 (en) *	2008-02-27	2009-09-03	Qteros, Inc.	Methods for the conversion of plant materials into fuels and chemicals by sequential action of two microorganisms
US8586828B2 (en) *	2008-03-06	2013-11-19	Novozymes A/S	Polypeptides having beta-glucosidase activity and polynucleotides encoding same
US20090286294A1 (en) *	2008-04-04	2009-11-19	University Of Massachusetts	Methods and Compositions for Improving the Production of Fuels in Microorganisms
US8212087B2 (en)	2008-04-30	2012-07-03	Xyleco, Inc.	Processing biomass
US8236535B2 (en)	2008-04-30	2012-08-07	Xyleco, Inc.	Processing biomass
BR122018010009B1 (pt)	2008-04-30	2021-05-04	Xyleco, Inc	Métodos de preparo de um material
US20100105114A1 (en) *	2008-06-11	2010-04-29	University Of Massachusetts	Methods and Compositions for Regulating Sporulation
EP2306964A1 (en) *	2008-07-03	2011-04-13	Novozymes A/S	A personal wash bar
WO2010059424A2 (en) *	2008-11-18	2010-05-27	Novozymes, Inc.	Methods and compositions for degrading cellulosic material
WO2010065830A1 (en)	2008-12-04	2010-06-10	Novozymes, Inc.	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
FR2939446B1 (fr)	2008-12-05	2011-04-22	Valagro Carbone Renouvelable	Utilisation de coton recycle pour produire de l'ethanol, et procede de production.
EP2379732A2 (en)	2008-12-19	2011-10-26	Novozymes Inc.	Methods for increasing enzymatic hydrolysis of cellulosic material in the presence of a peroxidase
UA117492C2 (uk)	2008-12-19	2018-08-10	Ксілеко, Інк.	Спосіб витягання вуглеводню із вуглеводеньвмісного матеріалу, вуглеводень, одержаний за його допомогою, та продукт, який містить вуглеводеньвмісний матеріал
US8338121B2 (en)	2008-12-19	2012-12-25	Novozymes, Inc.	Methods for determining cellulolytic enhancing activity of a polypeptide
CN102325879A (zh)	2008-12-19	2012-01-18	诺维信股份有限公司	在纤维二糖脱氢酶存在下增加纤维素材料水解的方法
US8337663B2 (en)	2008-12-19	2012-12-25	Novozymes, Inc.	Methods for increasing hydrolysis of cellulosic material
TWI384073B (zh) *	2008-12-24	2013-02-01	Taiwan Textile Res Inst	以含纖維素的纖維製品為原料生產生質酒精的方法
FI122029B (fi)	2008-12-30	2011-07-29	Ab Enzymes Oy	Sieniperäiset endoglukanaasit, niiden tuotto ja käyttö
US8604277B2 (en)	2009-01-28	2013-12-10	Novozymes, Inc.	Polypeptides having beta-glucosidase activity and polynucleotides encoding same
US8629324B2 (en)	2009-01-30	2014-01-14	Novozymes, Inc.	Polypeptides having expansin activity and polynucleotides encoding same
US20100086981A1 (en) *	2009-06-29	2010-04-08	Qteros, Inc.	Compositions and methods for improved saccharification of biomass
JP2012519500A (ja) *	2009-03-09	2012-08-30	クテロス，インコーポレイテッド	クロストリジウム（Ｃｌｏｓｔｒｉｄｉｕｍ）種からの発酵最終産物の生産
EP2411511B1 (en)	2009-03-24	2018-08-08	Novozymes A/S	Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2010138754A1 (en)	2009-05-29	2010-12-02	Novozymes, Inc.	Methods for enhancing the degradation or conversion of cellulosic material
WO2010141325A1 (en)	2009-06-02	2010-12-09	Novozymes, Inc.	Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8143021B2 (en)	2009-07-07	2012-03-27	Novozymes, Inc.	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US20110039319A1 (en) *	2009-08-12	2011-02-17	Theodora Retsina	Enzyme recycle from hydrolysis of lignocellulosic material
WO2011022644A2 (en) *	2009-08-21	2011-02-24	Auburn University	Fermentation and chemical treatment of pulp and paper mill sludge
WO2011035027A2 (en)	2009-09-17	2011-03-24	Novozymes, Inc.	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011035029A1 (en)	2009-09-18	2011-03-24	Novozymes, Inc.	Polypeptides having beta-glucosidase activity and polynucleotides encoding same
JP5932648B2 (ja)	2009-09-23	2016-06-15	ダニスコ・ユーエス・インク	新規なグリコシルヒドロラーゼ酵素及びその用途
US8211665B2 (en)	2009-09-29	2012-07-03	Novozymes, Inc.	Polypeptides having xylanase activity and polynucleotides encoding same
DK2483295T3 (en)	2009-09-29	2016-02-22	Novozymes Inc	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding them
CN108165542A (zh)	2009-09-30	2018-06-15	诺维信股份有限公司	具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
CN102666846A (zh)	2009-09-30	2012-09-12	诺维信公司	具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
CA2774142C (en)	2009-10-08	2021-06-29	Dsm Ip Assets B.V.	Process for the preparation of a fermentation product from lignocellulose containing material
US8541651B2 (en)	2009-10-23	2013-09-24	Novozymes, Inc.	Cellobiohydrolase variants and polynucleotides encoding same
CA2776336A1 (en)	2009-10-29	2011-05-19	Novozymes, Inc.	Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2496693B1 (en)	2009-11-06	2017-10-25	Novozymes, Inc.	Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2496692B1 (en)	2009-11-06	2016-03-16	Novozymes, Inc.	Polypeptides having xylanase activity and polynucleotides encoding same
MX2012005152A (es)	2009-11-06	2012-06-12	Novozymes Inc	Composiciones para sacrificacion de material celulosico.
CN102740868B (zh)	2009-11-25	2016-01-20	科德克希思公司	用于从纤维素生物质产生可溶性糖的重组β-葡糖苷酶变体
EP2504431A4 (en) *	2009-11-25	2013-10-16	Codexis Inc	RECOMBINANT BETA-GLUCOSIDASE VARIANTS OF THERMOASCUS AURIENTIACUS FOR THE PRODUCTION OF FERMENTABLE SUGARS FROM CELLULOSIC BIOMASS
EP2507430A4 (en) *	2009-12-04	2014-04-02	Georgia Tech Res Inst	Improved methods of treating a biomass for enzymatic hydrolysis
WO2011081658A2 (en) *	2009-12-15	2011-07-07	Qteros, Inc.	Methods and compositions for producing chemical products from c. phytofermentants
US20120276585A1 (en) *	2009-12-21	2012-11-01	Cofco Corporation	Method for producing fermentation products from lignocellulose-containing material
PH12012500999A1 (en)	2009-12-23	2013-02-11	Danisco Us Inc	Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
FI122937B (fi) *	2009-12-30	2012-09-14	Roal Oy	Menetelmä selluloosamateriaalin käsittelemiseksi sekä tässä käyttökelpoiset CBH II/Cel6A entsyymit
CN102858969B (zh) *	2010-01-28	2014-11-26	中央研究院	β-葡萄糖苷酶及其用途
EP2357227B1 (en)	2010-02-11	2015-07-15	Süd-Chemie IP GmbH & Co. KG	Optimized cellulase enzymes
GB2478791A (en) *	2010-03-19	2011-09-21	Qteros Inc	Ethanol production by genetically-modified bacteria
BR112012020503A2 (pt)	2010-03-31	2015-09-15	Novozymes Inc	variante isolado de celobioidrolase precursor, polipeptídeo isolado, construção de ácido nucleico, vetor de expressão, célula hospedeira, método de produzir um variante de celobioidrolas precursor, métodos para obter o variante, para degradar variante de celobioidrolase precursor, métodos para obter o variante, para degradar ou converter um material celulósico, poara produzir um produto de fermentação e de fermentar um material celulósico, e, composição de enzima.
EP2402454A1 (en) *	2010-06-30	2012-01-04	Süd-Chemie AG	Cellobiose production from biomass
DK2588604T3 (en)	2010-06-30	2016-09-26	Novozymes Inc	Polypeptides having beta-glucosidase activity and polynucleotides encoding them
US9273335B2 (en)	2010-08-12	2016-03-01	Novozymes, Inc.	Compositions comprising a polypeptide having cellulolytic enhancing activity and a quinone compound and uses thereof
DK2735611T3 (en)	2010-08-30	2019-01-28	Novozymes As	POLYPEPTIDES WITH CELLULOLYSE INCREASING ACTIVITY AND POLYNUCLEOTIDES CODING THEM
WO2012030858A2 (en)	2010-08-30	2012-03-08	Novozymes A/S	Polypeptides having hemicellulolytic activity and polynucleotides encoding same
WO2012030811A1 (en)	2010-08-30	2012-03-08	Novozymes A/S	Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8624082B2 (en)	2010-08-30	2014-01-07	Novozymes A/S	Polypeptides having xylanase activity and polynucleotides encoding same
WO2012030844A1 (en)	2010-08-30	2012-03-08	Novozymes A/S	Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2012030845A2 (en)	2010-08-30	2012-03-08	Novozymes A/S	Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
WO2012044835A1 (en)	2010-09-30	2012-04-05	Novozymes, Inc.	Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN103237891B (zh)	2010-09-30	2017-07-14	诺维信股份有限公司	具有纤维素分解增强活性的多肽变体及其编码多核苷酸
ES2563209T3 (es)	2010-10-01	2016-03-11	Novozymes, Inc.	Variantes de beta-glucosidasa y polinucleótidos que codifican las mismas
BR112013009817B1 (pt)	2010-10-26	2020-02-04	Novozymes As	métodos de degradar ou converter refugo de cana de açúcar, de produzir um produto de fermentação, e, de fermentar refugo de cana de açúcar
EP2635689B1 (en)	2010-11-02	2015-04-15	Novozymes, Inc.	Methods of pretreating cellulosic material with a gh61 polypeptide
US9212354B2 (en)	2010-11-04	2015-12-15	Novozymes Inc.	Polypeptides having cellobiohydrolase activitiy and polynucleotides encoding same
US9139823B2 (en)	2010-11-12	2015-09-22	Novozymes, Inc.	Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112013010129A2 (pt)	2010-11-18	2016-07-05	Novozymes Inc	polipeptídeo gh61 quimérico isolado, polinucleotídeo isolado, célula hospedeira, métodos para produzir um polipeptídeo gh61 quimérico, para degradas ou converter um material celulóstico, para sintetizar um produto de fermentação, para fermentar um material celulósico e para limpar ou lavar uma superfície dura ou roupa para lavar, planta transgênica, parte da planta ou célula de planta, uso do polipeptídeo gh61 quimérico, composição detergente, e, formulação de caldo ou composição de cultura de células completas
EP2649188A1 (en)	2010-12-06	2013-10-16	Novozymes North America, Inc.	Methods of hydrolyzing oligomers in hemicellulosic liquor
MX337913B (es)	2011-01-26	2016-03-28	Novozymes As	Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican los mismos.
US9080161B2 (en)	2011-01-26	2015-07-14	Novozymes, Inc.	Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012103322A1 (en)	2011-01-26	2012-08-02	Novozymes A/S	Polypeptides having endoglucanase activity and polynucleotides encoding same
MX2013007720A (es)	2011-01-26	2013-08-09	Novozymes As	Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
CN103534348B (zh)	2011-01-26	2018-04-10	诺维信公司	具有纤维二糖水解酶活性的多肽和编码该多肽的多核苷酸
BR112013019324B1 (pt)	2011-01-31	2021-06-08	Novozymes North America, Inc	processos para refinamento enzimático de um material celulósico pré-tratado
MX2013007997A (es)	2011-02-23	2013-08-21	Novozymes Inc	Polipeptidos que tienen actividad de incremento celulolitico y polinucleotidos que codifican para los mismos.
EP2683830A1 (en)	2011-03-09	2014-01-15	Novozymes A/S	Methods of increasing the cellulolytic enhancing activity of a polypeptide
WO2012122477A1 (en)	2011-03-10	2012-09-13	Novozymes A/S	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
MX2013010512A (es) *	2011-03-17	2013-10-07	Danisco Inc	Metodo para reducir la viscosidad en un proceso de sacarificacion.
US9879294B2 (en)	2011-03-25	2018-01-30	Novozymes A/S	Methods for degrading or converting cellulosic material
WO2012135719A1 (en)	2011-03-31	2012-10-04	Novozymes, Inc.	Cellulose binding domain variants and polynucleotides encoding same
US9410136B2 (en)	2011-03-31	2016-08-09	Novozymes, Inc.	Methods for enhancing the degradation or conversion of cellulosic material
US9926547B2 (en)	2011-04-28	2018-03-27	Novozymes, Inc.	Polypeptides having endoglucanase activity and polynucleotides encoding same
CN103797126A (zh)	2011-04-29	2014-05-14	诺维信股份有限公司	用于增强纤维素材料的降解或转化的方法
US20140141471A1 (en)	2011-05-19	2014-05-22	Novozymes, Inc.	Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins
WO2012159009A1 (en)	2011-05-19	2012-11-22	Novozymes, Inc.	Methods for enhancing the degradation of cellulosic material with chitin binding proteins
DK2734633T3 (da)	2011-07-22	2019-06-11	Novozymes North America Inc	Fremgangsmåder til forbehandling af celluloseholdigt materiale og forbedring af hydrolyse deraf
US8859227B2 (en)	2011-08-04	2014-10-14	Novozymes, Inc.	Polypeptides having xylanase activity and polynucleotides encoding same
CN103703125B (zh)	2011-08-04	2016-09-07	诺维信公司	具有内切葡聚糖酶活性的多肽及其编码多核苷酸
AU2012298799B2 (en)	2011-08-24	2018-02-01	Novozymes, Inc.	Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
CN103748226A (zh)	2011-08-24	2014-04-23	诺维信股份有限公司	获得丝状真菌宿主细胞的阳性转化体的方法
EP2756091A1 (en)	2011-09-13	2014-07-23	Novozymes North America, Inc.	Methods of hydrolyzing and fermenting cellulosic material
US20140308705A1 (en)	2011-09-20	2014-10-16	Novozymes A/S	Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
EP2748313B1 (en) *	2011-09-23	2016-04-13	Novozymes A/S	Cellulolytic enzyme compositions and uses thereof
WO2013089889A2 (en)	2011-09-30	2013-06-20	Novozymes, Inc.	Chimeric polypeptides having beta-glucosidase activity and polynucleotides encoding same
US10704034B2 (en)	2011-10-28	2020-07-07	Novozymes Inc.	Polypeptides having xylanase activity and polynucleotides encoding same
BR112014008056A2 (pt) *	2011-10-28	2017-04-11	Novozymes Inc	polipeptídeo isolado, polinucleotídeo isolado, célula hospedeira recombinante, métodos para produzir um polipeptídeo, um mutante de uma célula precursora, e uma proteína, planta transgênica, parte de planta ou célula de planta, molécula de rna inibitório dupla fita, processos para sintetizar um produto de fermentação, e para fermentar um material celulósico ou contendo xilano, e, formulação de caldo completo ou composição de cultura de células
CN104039817A (zh) *	2011-10-28	2014-09-10	诺维信股份有限公司	具有木聚糖酶活性的多肽和编码该多肽的多核苷酸
DK2773656T3 (da)	2011-10-31	2019-09-09	Novozymes Inc	Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem
CN104350148A (zh) *	2011-11-15	2015-02-11	诺维信股份有限公司	具有纤维二糖水解酶活性的多肽和编码该多肽的多核苷酸
BR112014011399A2 (pt) *	2011-11-15	2017-06-06	Novozymes Inc	“polipeptídeo isolado com atividade de celobiohidrolase, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, formulação de caldo completo ou composição de cultura de células, e, processos para degradar ou converter um material celulósico e para produzir um produto de fermentação
US9562222B2 (en)	2011-11-18	2017-02-07	Novozymes A/S	Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
EP3597736A1 (en)	2011-11-21	2020-01-22	Novozymes A/S	Gh61 polypeptide variants and polynucleotides encoding same
EP2782998B1 (en)	2011-11-22	2018-01-10	Novozymes Inc.	Polypeptides having beta-xylosidase activity and polynucleotides encoding same
DK2785732T3 (en)	2011-12-01	2017-06-19	Novozymes Inc	POLYPEPTIDES WITH BETA-XYLOSIDASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
WO2013087027A1 (en)	2011-12-16	2013-06-20	Novozymes, Inc.	Polypeptides having laccase activity and polynucleotides encoding same
EP2794870A4 (en)	2011-12-19	2015-06-17	Novozymes Inc	POLYPEPTIDES WITH XYLANASE ACTIVITY AND POLYNUCLEOTIDES THAT CODE
US20140342038A1 (en)	2011-12-19	2014-11-20	Novozymes A/S	Processes and Compositions For Increasing The Digestibility of Cellulosic Materials
US10036050B2 (en)	2011-12-20	2018-07-31	Novozymes, Inc.	Cellobiohydrolase variants and polynucleotides encoding same
US20150017670A1 (en)	2011-12-21	2015-01-15	Novozymes, Inc.	Methods For Determining The Degradation Of A Biomass Material
BR112014015242B1 (pt)	2011-12-22	2023-01-31	Novozymes, Inc	Polipeptídeo híbrido isolado, polinucleotídeo isolado,célula hospedeira, método de produção de um polipeptídeo híbrido, processos para degradação ou conversão de um material celulósico, para produção de um produto de fermentação, de fermentação de um material celulósico, formulação ou composição de cultura de células microbianas de caldo completo, e,célula hospedeira microbiana transgênica
CN102618602B (zh) *	2012-03-30	2013-05-15	吴允山	甘薯渣酶法水解糖工艺
CN104245930A (zh)	2012-04-23	2014-12-24	诺维信公司	具有葡萄糖醛酸酯酶活性的多肽以及编码其的多核苷酸
WO2013160248A2 (en)	2012-04-23	2013-10-31	Novozymes A/S	Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
CN119193513A (zh)	2012-04-27	2024-12-27	诺维信股份有限公司	Gh61多肽变体以及编码其的多核苷酸
FI124476B (en) *	2012-05-24	2014-09-15	Roal Oy	Improved endoglucanases for handling cellulosic material
US8975058B2 (en)	2012-05-24	2015-03-10	Roal Oy	Endoglucanases for treatment of cellulosic material
FI124477B (en) *	2012-06-07	2014-09-15	Roal Oy	New proteins for the treatment of cellulose materials
US9458440B2 (en)	2012-06-07	2016-10-04	Roal Oy	Proteins for the treatment of cellulosic material
EA201590312A1 (ru) *	2012-08-03	2015-10-30	ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС	Ферменты
EP2898068A2 (en)	2012-09-19	2015-07-29	Novozymes, Inc.	Methods for enhancing the degradation or conversion of cellulosic material
US10035996B2 (en)	2012-10-08	2018-07-31	Novozymes, Inc.	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US20150275194A1 (en)	2012-10-24	2015-10-01	Novozymes A/S	Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
US9434929B2 (en)	2012-10-26	2016-09-06	Roal Oy	Esterases useful in the treatment of cellulosic and lignocellulosic material
EP2914717A1 (en) *	2012-10-31	2015-09-09	Danisco US Inc.	Beta-glucosidase from magnaporthe grisea
WO2014093835A1 (en)	2012-12-14	2014-06-19	Novozymes A/S	Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2014099798A1 (en)	2012-12-19	2014-06-26	Novozymes A/S	Polypeptides having cellulolytic enhancinc activity and polynucleotides encoding same
CN103013959A (zh) *	2013-01-17	2013-04-03	青岛农业大学	一种用于降解纤维素的混合酶
US8753860B1 (en) *	2013-02-12	2014-06-17	Novozymes A/S	Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN110628749A (zh)	2013-03-08	2019-12-31	诺维信公司	纤维二糖水解酶变体和编码它们的多核苷酸
US9617574B2 (en)	2013-03-15	2017-04-11	Auburn University	Efficient process for producing saccharides and ethanol from a biomass feedstock
US9850512B2 (en)	2013-03-15	2017-12-26	The Research Foundation For The State University Of New York	Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
FI126698B (en) *	2013-12-18	2017-04-13	Teknologian Tutkimuskeskus Vtt Oy	Currently for the production of fibrillated cellulose material
CN105745331B (zh)	2013-12-27	2019-12-20	东丽株式会社	糖液的制造方法
US9951363B2 (en)	2014-03-14	2018-04-24	The Research Foundation for the State University of New York College of Environmental Science and Forestry	Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
KR20170026521A (ko) *	2014-06-25	2017-03-08	제이에이치엘 바이오테크 인코포레이티드	단백질의 정제를 위한 방법 및 시약
SG11201610894UA (en) *	2014-08-08	2017-01-27	Xyleco Inc	Aglycosylated enzyme and uses thereof
EP3594335B1 (en)	2014-09-05	2024-05-01	Novozymes A/S	Carbohydrate binding module variants and polynucleotides encoding same
FI127093B (en)	2014-10-27	2017-11-15	Ab Enzymes Oy	Fungal-derived endoglucanase variants, their production and use
US10465214B2 (en)	2014-11-20	2019-11-05	Full Cycle Bioplastics Llc	Producing resins from organic waste products
PL3640336T3 (pl)	2015-01-28	2022-08-01	Dsm Ip Assets B.V.	Sposób enzymatycznej hydrolizy materiału lignocelulozowego i fermentacji cukrów
PL3250698T3 (pl)	2015-01-28	2020-06-29	Dsm Ip Assets B.V.	Sposób enzymatycznej hydrolizy materiału lignocelulozowego i fermentacji cukrów
WO2016120298A1 (en)	2015-01-28	2016-08-04	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
DK3262165T3 (da)	2015-02-24	2020-08-24	Novozymes As	Cellobiohydrolasevarianter og polynukleotider, som koder for dem
EP3067428A1 (en)	2015-03-12	2016-09-14	BETA RENEWABLES S.p.A.	A process for producing a hydrolyzed mixture from a pre-treated ligno-cellulosic slurry comprising a slurry liquid and slurry solids
BR112017017895A2 (pt)	2015-03-16	2018-04-10	Iogen Corp	processo para produzir um produto de fermentação e para produzir um álcool a partir de uma matéria prima lignocelulósica.
US10421667B2 (en)	2015-03-16	2019-09-24	Iogen Corporation	Process for treating lignocellulosic feedstock comprising wet oxidation
US10995314B2 (en)	2015-03-16	2021-05-04	Iogen Corporation	Sulfur dioxide and/or sulfurous acid pretreatment with sulfur dioxide recovery
WO2016169893A1 (en)	2015-04-20	2016-10-27	Dsm Ip Assets B.V.	Whole fermentation broth
WO2016169892A1 (en)	2015-04-20	2016-10-27	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016207144A1 (en)	2015-06-22	2016-12-29	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CN105058546B (zh) *	2015-08-06	2018-05-08	浙江品创知识产权服务有限公司	一种酶处理环保松木板
EP3390625B1 (en)	2015-12-18	2023-09-06	Danisco US Inc.	Polypeptides with endoglucanase activity and uses thereof
US10662455B2 (en)	2015-12-18	2020-05-26	Iogen Corporation	Sulfur dioxide and/or sulfurous acid pretreatment
BR112018016366A2 (pt)	2016-02-10	2018-12-18	Iogen Corp	processos para hidrolisar biomassa lignocelulósica e para pré-tratamento de biomassa lignocelulósica.
CA3010435A1 (en) *	2016-02-10	2017-08-17	Novozymes A/S	Preparation of a baked product comprising fibers treated by a cellulase
EP3469090A1 (en)	2016-06-09	2019-04-17	DSM IP Assets B.V.	Seed train for large scale enzyme production
WO2018019948A1 (en)	2016-07-29	2018-02-01	Dsm Ip Assets B.V.	Polypeptides having cellulolytic enhancing activity and uses thereof
AT519059B1 (de) *	2016-09-02	2018-07-15	Andritz Ag Maschf	Verfahren zur Abtrennung von Hemizellulosen aus Biomasse-Aufschlussprozesswasser oder verbrauchten Laugen
US11499142B2 (en)	2016-11-24	2022-11-15	Dsm Ip Assets B.V.	Enzyme composition
CA3043435A1 (en)	2016-11-24	2018-05-31	Dsm Ip Assets B.V.	Enzyme compositions with improved hydrolysis performance
WO2018185071A1 (en)	2017-04-03	2018-10-11	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CN108866025B (zh) *	2017-05-10	2021-04-13	中国科学院青岛生物能源与过程研究所	一种纤维素酶制剂及其应用
FI3695001T3 (fi)	2017-10-09	2024-02-14	Versalis Spa	Menetelmä lignoselluloosamateriaalin entsymaattista hydrolyysiä ja sokerien fermentointia varten
JP2021501231A (ja)	2017-10-27	2021-01-14	キシレコインコーポレイテッド	バイオマスの加工方法
WO2019086370A1 (en)	2017-10-30	2019-05-09	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019086369A1 (en)	2017-10-30	2019-05-09	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019090414A1 (en)	2017-11-09	2019-05-16	Iogen Corporation	Low temperature pretreatment with sulfur dioxide
EP3707269A4 (en)	2017-11-09	2021-08-25	Iogen Corporation	PRE-TREATMENT OF SULFUR DIOXIDE AT LOW TEMPERATURE
EP3530743A1 (en)	2018-02-21	2019-08-28	Cambridge Glycoscience Ltd	Method of production
WO2019185681A1 (en)	2018-03-28	2019-10-03	Dsm Ip Assets B.V.	Enzyme composition
BR112020018791A2 (pt)	2018-03-28	2020-10-13	Dsm Ip Assets B.V.	composição de enzima
CA3094413A1 (en)	2018-04-06	2019-10-10	Iogen Corporation	Pretreatment with lignosulfonic acid
US20190328619A1 (en)	2018-04-27	2019-10-31	Pilleve, Inc.	Pill dispensing system
BR112020023198A2 (pt)	2018-05-17	2021-02-09	Dsm Ip Assets B.V.	processo para produção de um polipeptídeo
EP3802843B1 (en)	2018-05-30	2023-03-29	Versalis S.p.A.	Process for producing sugars from carbohydrate materials
CN108841810B (zh) *	2018-07-21	2021-08-27	湖北大学	一种多功能纤维素酶基因及其应用
JP7374991B2 (ja)	2018-08-15	2023-11-07	ケンブリッジグリコサイエンスエルティーディー	新規組成物、それらの使用、およびそれらの形成方法
CN108976302B (zh) *	2018-08-17	2021-04-13	中国科学院青岛生物能源与过程研究所	用于催化木质纤维素糖化的纤维小体酶制剂
WO2020058248A1 (en)	2018-09-18	2020-03-26	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058253A1 (en)	2018-09-18	2020-03-26	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058249A1 (en)	2018-09-18	2020-03-26	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020083951A1 (en)	2018-10-24	2020-04-30	Dsm Ip Assets B.V.	Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
RU2696074C1 (ru) *	2018-11-16	2019-07-30	Федеральное государственное бюджетное учреждение науки Федеральный исследовательский центр питания, биотехнологии и безопасности пищи	Штамм мицелиального гриба trichoderma reesei - продуцент комплекса эндоглюканазы, ксиланазы и пектиназ для получения белковых добавок на основе зернового и зернобобового сырья для применения в кормопроизводстве
CN113544280A (zh)	2019-03-12	2021-10-22	帝斯曼知识产权资产管理有限公司	用于产生发酵液的方法
US12232512B2 (en) *	2019-06-05	2025-02-25	Danisco Us Inc.	Methods for improving the amino acid content of animal feed products
WO2021032647A1 (en)	2019-08-16	2021-02-25	Cambridge Glycoscience Ltd	Methods of treating biomass to produce oligosaccharides and related compositions
CN114391041A (zh) *	2019-09-10	2022-04-22	帝斯曼知识产权资产管理有限公司	酶组合物
US20220364131A1 (en) *	2019-10-04	2022-11-17	Sharetex Ab	Process for manufacturing organic chemicals and/or distillate hydrocarbon fuels from waste textiles
WO2021116437A2 (en)	2019-12-12	2021-06-17	Cambridge Glycoscience Ltd	Low sugar multiphase foodstuffs
WO2022013148A1 (en)	2020-07-13	2022-01-20	Dsm Ip Assets B.V.	Process for the production of biogas
WO2022214459A1 (en)	2021-04-06	2022-10-13	Dsm Ip Assets B.V.	Enzyme composition
CN117178059A (zh)	2021-04-06	2023-12-05	帝斯曼知识产权资产管理有限公司	酶组合物
BR112023020370A2 (pt)	2021-04-06	2023-11-21	Dsm Ip Assets Bv	Composição de enzimas
CA3197143A1 (en)	2021-04-08	2022-10-13	Versalis S.P.A.	Process for the preparation of a sugar product and a fermentation product
TWI796066B (zh) *	2021-12-27	2023-03-11	行政院原子能委員會核能研究所	廢紙容器處理方法及使用廢紙容器水解液生產聚羥基烷酸酯的方法
US20250311734A1 (en)	2022-05-14	2025-10-09	Novozymes A/S	Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
CN121472198B (zh) *	2026-01-12	2026-03-20	上海康地恩生物科技有限公司	一种高比活纤维素酶突变体及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1996029397A1 (en) *	1995-03-17	1996-09-26	Novo Nordisk A/S	Novel endoglucanases
WO2003000941A2 (en) *	2001-06-26	2003-01-03	Novozymes A/S	Polypeptides having cellobiohydrolase i activity and polynucleotides encoding same
WO2003027306A2 (en) *	2001-09-21	2003-04-03	Genencor International, Inc.	Bgl3 beta-glucosidase and nucleic acids encoding the same
US20040053373A1 (en) *	2000-09-25	2004-03-18	Brian Foody	Method for glucose production with improved recovery and reuse of enzyme

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4966850A (en) *	1987-01-21	1990-10-30	Forintek Canada Corp.	Production of thermostable xylanase and cellulase
DK115890D0 (da)	1990-05-09	1990-05-09	Novo Nordisk As	Enzym
CA2082279C (en)	1990-05-09	2007-09-04	Grethe Rasmussen	Cellulase preparation comprising an endoglucanase enzyme
US5997913A (en)	1990-12-10	1999-12-07	Genencor International, Inc.	Method enhancing flavor and aroma in foods by overexpression of β-glucosidase
DK0562003T4 (en)	1990-12-10	2015-07-13	Danisco Us Inc	Improved saccharification of cellulose by cloning and amplification of.-Glucosidase gene from Tricodermareesei
DE69133035T2 (de)	1991-01-16	2003-02-13	The Procter & Gamble Company, Cincinnati	Kompakte Waschmittelzusammensetzungen mit hochaktiven Cellulasen
EP1479765B1 (en)	1993-03-10	2008-10-22	Novozymes A/S	Enzymes with xylanase activity from aspergillus aculeatus
US5912157A (en)	1994-03-08	1999-06-15	Novo Nordisk A/S	Alkaline cellulases
JP3107977B2 (ja) *	1994-08-29	2000-11-13	明治製菓株式会社	新規セルラーゼおよびその遺伝子
US5919691A (en)	1994-10-06	1999-07-06	Novo Nordisk A/S	Enzyme and enzyme preparation with endoglucanase activity
AU7294396A (en)	1995-10-13	1997-04-30	Gist-Brocades B.V.	Protein detection
US6723549B2 (en) *	1995-10-17	2004-04-20	Ab Enzymes Oy	Cellulases, the genes encoding them and uses thereof
GB9617184D0 (en)	1996-08-15	1996-09-25	Danisco	Enzyme
BR9711479B1 (pt) *	1996-09-17	2009-08-11		variante de celulase tendo uma resistência aumentada a tensìdeo de ánion.
US5811381A (en) *	1996-10-10	1998-09-22	Mark A. Emalfarb	Cellulase compositions and methods of use
EP0843041A1 (en)	1996-11-13	1998-05-20	Novo Nordisk A/S	Garments with considerable variation in abrasion level and process for its production using cellulolytic enzymes
FR2786784B1 (fr)	1998-12-04	2003-01-24	Lesaffre & Cie	FRAGMENT D'ADN CODANT POUR LA XYLANASE THERMOSTABLE XynA DE THERMOASCUS
AU1685600A (en) *	1998-12-24	2000-07-31	Takara Shuzo Co., Ltd.	Polypeptides
GB9928968D0 (en)	1999-12-07	2000-02-02	Danisco	Enzyme
ES2315272T3 (es)	2000-02-17	2009-04-01	Technical University Of Denmark	Un procedimiento para procesar material lignocelulosico.
CA2689798C (en)	2000-03-20	2015-01-20	Basf Aktiengesellschaft	Talaromyces emersonii beta-glucanases
CZ2003798A3 (cs)	2000-09-21	2003-08-13	Dsm N. V.	Polypeptid s aktivitou xylanázy, sekvence nukleové kyseliny kódující polypeptid, způsob přípravy a použití polypeptidu
AU2001292292A1 (en)	2000-09-29	2002-04-08	Meiji Seika Kaisha Ltd.	Novel enzyme having beta-glucosidase activity and use thereof
TWI301412B (en) *	2001-04-09	2008-10-01	Lonza Ag	Pharmaceutical composition and injection solution for improving the well-being of poultry
JP2004527261A (ja)	2001-05-18	2004-09-09	ノボザイムスアクティーゼルスカブ	セロビアーゼ活性を有するポリペプチド及びそれをコードするポリヌクレオチド
US20060193843A1 (en)	2002-01-25	2006-08-31	Dsm Ip Assets B.V.	Thermostable enzyme compositions
CA2479248C (en)	2002-03-15	2011-09-27	Iogen Energy Corporation	Method for glucose production using endoglucanase core protein for improved recovery and reuse of enzyme
US20040005674A1 (en)	2002-04-30	2004-01-08	Athenix Corporation	Methods for enzymatic hydrolysis of lignocellulose
JP4756861B2 (ja) *	2002-08-16	2011-08-24	ジェネンコー・インターナショナル・インク	新規なハイプロクレアジェコリーナｃｂｈ１セルラーゼ
US20060035800A1 (en) *	2002-12-11	2006-02-16	Novozymes A/S	Detergent composition
US7348168B2 (en) *	2002-12-20	2008-03-25	Novozymes A/S	Polypeptides having cellobiohydrolase II activity and polynucleotides encoding same
JP5427342B2 (ja) *	2003-04-01	2014-02-26	ジェネンコー・インターナショナル・インク	変異体フミコーラ・グリセアｃｂｈ１．１
EP1682656B1 (en)	2003-10-28	2013-09-18	Novozymes Inc.	Polypeptides having beta-glucosidase activity and polynucleotides encoding same
ATE489457T1 (de)	2004-01-16	2010-12-15	Novozymes Inc	Verfahren zum abbau von lignocellulosematerialien
ES2389442T3 (es)	2004-02-06	2012-10-26	Novozymes Inc.	Polipéptidos con actividad de aumento celulolítica y polinucleótidos que los codifican
US8097445B2 (en) *	2004-03-25	2012-01-17	Danisco Us Inc.	Exo-endo cellulase fusion protein
WO2005100557A1 (en)	2004-04-16	2005-10-27	Ab Enzymes Oy	Method and dna constructs for increasing the production level of carbohydrate degrading enzymes in filamentous fungi
FI118012B (fi)	2004-06-04	2007-05-31	Valtion Teknillinen	Menetelmä etanolin valmistamiseksi
BRPI0609906A8 (pt) *	2005-04-29	2017-11-21	Ab Enzymes Oy	proteína de fusão de celulase, seu processo de produção e composição detergente contendo a mesma, vetor de expressão, célula hospedeira, preparação de enzima, processos para desbotamento e para bioacabamento e métodos de tratamento de fibra celulósica contendo material têxtil, para tratar fibra ou polpa derivada de madeira e para melhorar a qualidade de ração animal
US7741093B2 (en) *	2005-04-29	2010-06-22	Ab Enzymes Oy	Cellulases and their uses
FI118979B (fi)	2005-04-29	2008-05-30	Metso Automation Oy	Menetelmä sakeuden mittaamiseksi, katkojarakenne ja sakeusmittari
US7256032B2 (en)	2005-12-22	2007-08-14	Ab Enzymes Oy	Enzymes
TWI625390B (zh)	2013-03-14	2018-06-01	安美基公司	用於增加重組蛋白質之甘露糖含量之方法

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- 2005-12-22 FI FI20051318A patent/FI120045B/fi active IP Right Grant
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- 2010-11-02 US US12/917,603 patent/US8409836B2/en not_active Expired - Fee Related
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- 2013-02-22 US US13/774,465 patent/US9758777B2/en active Active
- 2013-10-03 US US14/045,236 patent/US9593324B2/en active Active
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- 2017-08-30 US US15/690,513 patent/US20180044656A1/en not_active Abandoned

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1996029397A1 (en) *	1995-03-17	1996-09-26	Novo Nordisk A/S	Novel endoglucanases
US20040053373A1 (en) *	2000-09-25	2004-03-18	Brian Foody	Method for glucose production with improved recovery and reuse of enzyme
WO2003000941A2 (en) *	2001-06-26	2003-01-03	Novozymes A/S	Polypeptides having cellobiohydrolase i activity and polynucleotides encoding same
WO2003027306A2 (en) *	2001-09-21	2003-04-03	Genencor International, Inc.	Bgl3 beta-glucosidase and nucleic acids encoding the same

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EP2319920B1 (en)	2014-12-03
CN102174492B (zh)	2014-01-01
BRPI0620287B1 (pt)	2018-12-18
EP2453014B1 (en)	2016-06-08
CA2632502A1 (en)	2007-06-28
RU2458128C2 (ru)	2012-08-10
CN101384713B (zh)	2013-03-20
US9758777B2 (en)	2017-09-12
AU2006326963A1 (en)	2007-06-28
EP2319920A1 (en)	2011-05-11
US20140322762A1 (en)	2014-10-30
FI20051318A0 (fi)	2005-12-22
FI20051318L (fi)	2006-12-28
AP2008004509A0 (en)	2008-06-30
CA2833029A1 (en)	2007-06-28
ES2582078T3 (es)	2016-09-09
US20130224801A1 (en)	2013-08-29
DK2453014T3 (en)	2016-08-01
US20180044656A1 (en)	2018-02-15
BRPI0620287A2 (pt)	2011-11-08
EP1963498A4 (en)	2010-01-06
AP2924A (en)	2014-05-31
ES2501944T9 (es)	2014-10-22
RU2008123954A (ru)	2010-01-27
ES2527681T3 (es)	2015-01-28
EP1963498B1 (en)	2014-08-13
US9593324B2 (en)	2017-03-14
EP1963498A1 (en)	2008-09-03
EP2453013A9 (en)	2012-11-28
ES2582320T3 (es)	2016-09-12
CN102174492A (zh)	2011-09-07
AR058721A1 (es)	2008-02-20
DK2453013T3 (en)	2016-07-25
EP2453014A1 (en)	2012-05-16
US20090042266A1 (en)	2009-02-12
CA2833029C (en)	2018-03-27
TW200801195A (en)	2008-01-01
AU2006326963B2 (en)	2012-03-29
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US8409836B2 (en)	2013-04-02
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US20110045544A1 (en)	2011-02-24
CN101384713A (zh)	2009-03-11
WO2007071818A1 (en)	2007-06-28
EP2453013B1 (en)	2016-06-08
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US9593324B2 (en)	2017-03-14	Treatment of cellulosic material and enzymes useful therein
US8580552B2 (en)	2013-11-12	Method for treating cellulosic material and CBHII/CEL6A enzymes useful therein
CA2994320C (en)	2023-05-16	Treatment of cellulosic material and enzymes useful therein
Class et al.	2014	Patent application title: Treatment of Cellulosic Material and Enzymes Useful Therein Inventors: Jari Vehmaanperä (Klaukkala, FI) Jari Vehmaanperä (Klaukkala, FI) Marika Alapuranen (Rajamaki, FI) Terhi Puranen (Nurmijarvi, FI) Terhi Puranen (Nurmijarvi, FI) Matti Siika-Aho (Helsinki, FI) Jarno Kallio (Jarvenpaa, FI) Jarno Kallio (Jarvenpaa, FI) Satu Hooman (Espoo, FI) Sanni Voutilainen (Lohja, FI) Teemu Halonen (Espoo, FI) Liisa Viikari (Helsinki, FI) Assignees: Roal OY