UA49843C2 - Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти - Google Patents

Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти

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Publication number
UA49843C2
UA49843C2 UA98031382A UA98031382A UA49843C2 UA 49843 C2 UA49843 C2 UA 49843C2 UA 98031382 A UA98031382 A UA 98031382A UA 98031382 A UA98031382 A UA 98031382A UA 49843 C2 UA49843 C2 UA 49843C2
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UA
Ukraine
Prior art keywords
amplification
nucleic acid
oligonucleotide
acid sequence
directed
Prior art date
Application number
UA98031382A
Other languages
English (en)
Russian (ru)
Inventor
Стівен Гордон Уілл
Original Assignee
Ф.Хоффманн-Ля Рош Аг
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Publication date
Application filed by Ф.Хоффманн-Ля Рош Аг filed Critical Ф.Хоффманн-Ля Рош Аг
Publication of UA49843C2 publication Critical patent/UA49843C2/uk

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Paints Or Removers (AREA)
  • Dental Preparations (AREA)
  • Saccharide Compounds (AREA)

Abstract

Винахід забезпечує модифіковані олігонуклеотиди для ампліфікації послідовності нуклеїнової кислоти. Наслідком ампліфікацій, здійснених з використанням модифікованих олігонуклеотидів, є менша кількість неспецифічного продукту ампліфікації, зокрема праймеру-димеру, і супровідний більший вихід передбачуваного продукту ампліфікації у порівнянні з ампліфікаціями, здійсненими з використанням немодифікованих олігонуклеотидів, як праймерів.
UA98031382A 1997-03-20 1998-03-19 Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти UA49843C2 (uk)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4112797P 1997-03-20 1997-03-20

Publications (1)

Publication Number Publication Date
UA49843C2 true UA49843C2 (uk) 2002-10-15

Family

ID=21914898

Family Applications (1)

Application Number Title Priority Date Filing Date
UA98031382A UA49843C2 (uk) 1997-03-20 1998-03-19 Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти

Country Status (20)

Country Link
US (1) US6001611A (uk)
EP (1) EP0866071B1 (uk)
JP (1) JP2966389B2 (uk)
KR (1) KR100292997B1 (uk)
CN (1) CN1065873C (uk)
AT (1) ATE280177T1 (uk)
AU (1) AU712448B2 (uk)
BR (1) BR9801878B1 (uk)
CA (1) CA2229766C (uk)
CZ (1) CZ291525B6 (uk)
DE (1) DE69827060T2 (uk)
DK (1) DK0866071T3 (uk)
ES (1) ES2230631T3 (uk)
HU (1) HU223669B1 (uk)
IL (1) IL123694A (uk)
NO (1) NO322136B1 (uk)
PL (1) PL190142B1 (uk)
RU (1) RU2159248C2 (uk)
TW (1) TW567188B (uk)
UA (1) UA49843C2 (uk)

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