UA80809C2 - Process for preparation of protective antigen protein of anthracis with e.coli - Google Patents

Process for preparation of protective antigen protein of anthracis with e.coli Download PDF

Info

Publication number: UA80809C2
Authority: UA; Ukraine
Prior art keywords: cells; medium; protective antigen; protein; recombinant
Prior art date: 2001-07-12

Application number

UA20040503327A

Other languages

English (en)

Ukrainian (uk)

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-11-05

Filing date

2001-07-12

Publication date

2007-11-12

2001-07-12 Application filed filed Critical

2007-11-12 Publication of UA80809C2 publication Critical patent/UA80809C2/uk

Links

238000000034 method Methods 0.000 title claims abstract description 34
101710194807 Protective antigen Proteins 0.000 title claims abstract description 22
241000588724 Escherichia coli Species 0.000 title abstract description 5
238000002360 preparation method Methods 0.000 title abstract description 5
230000008569 process Effects 0.000 title abstract description 4
241000193738 Bacillus anthracis Species 0.000 claims abstract description 8
108091007433 antigens Proteins 0.000 claims abstract description 3
102000036639 antigens Human genes 0.000 claims abstract description 3
210000004027 cell Anatomy 0.000 claims description 69
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 45
108090000623 proteins and genes Proteins 0.000 claims description 34
102000004169 proteins and genes Human genes 0.000 claims description 30
235000010469 Glycine max Nutrition 0.000 claims description 18
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 18
238000004113 cell culture Methods 0.000 claims description 16
239000013612 plasmid Substances 0.000 claims description 16
244000068988 Glycine max Species 0.000 claims description 15
230000014509 gene expression Effects 0.000 claims description 15
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 13
239000001301 oxygen Substances 0.000 claims description 13
229910052760 oxygen Inorganic materials 0.000 claims description 13
238000000855 fermentation Methods 0.000 claims description 12
230000004151 fermentation Effects 0.000 claims description 12
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
239000004202 carbamide Substances 0.000 claims description 10
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OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 7
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 7
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150000001720 carbohydrates Chemical class 0.000 claims description 6
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229920005862 polyol Polymers 0.000 claims description 6
BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 5
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 5
BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 5
GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 5
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RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 4
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WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 4
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230000007928 solubilization Effects 0.000 claims description 2
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230000000890 antigenic effect Effects 0.000 claims 1
238000001962 electrophoresis Methods 0.000 claims 1
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229940065181 bacillus anthracis Drugs 0.000 abstract 1
239000002609 medium Substances 0.000 description 23
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 18
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235000011187 glycerol Nutrition 0.000 description 15
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239000000758 substrate Substances 0.000 description 10
230000015572 biosynthetic process Effects 0.000 description 9
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239000000047 product Substances 0.000 description 8
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 8
239000001963 growth medium Substances 0.000 description 7
238000004519 manufacturing process Methods 0.000 description 7
238000000746 purification Methods 0.000 description 5
239000000523 sample Substances 0.000 description 5
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230000012010 growth Effects 0.000 description 4
229910000160 potassium phosphate Inorganic materials 0.000 description 4
235000011009 potassium phosphates Nutrition 0.000 description 4
239000013049 sediment Substances 0.000 description 4
239000000725 suspension Substances 0.000 description 4
241000894006 Bacteria Species 0.000 description 3
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
238000009825 accumulation Methods 0.000 description 3
229960002235 anthrax antigen Drugs 0.000 description 3
239000002518 antifoaming agent Substances 0.000 description 3
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RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
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238000002955 isolation Methods 0.000 description 3
238000013518 transcription Methods 0.000 description 3
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IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
102000035195 Peptidases Human genes 0.000 description 2
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239000004365 Protease Substances 0.000 description 2
238000010521 absorption reaction Methods 0.000 description 2
238000001042 affinity chromatography Methods 0.000 description 2
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
229960000723 ampicillin Drugs 0.000 description 2
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230000004071 biological effect Effects 0.000 description 2
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239000013578 denaturing buffer Substances 0.000 description 2
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239000003814 drug Substances 0.000 description 2
239000012535 impurity Substances 0.000 description 2
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239000002244 precipitate Substances 0.000 description 2
230000000241 respiratory effect Effects 0.000 description 2
230000004044 response Effects 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
239000000243 solution Substances 0.000 description 2
238000012360 testing method Methods 0.000 description 2
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238000013519 translation Methods 0.000 description 2
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JDIIGWSSTNUWGK-UHFFFAOYSA-N 1h-imidazol-3-ium;chloride Chemical compound [Cl-].[NH2+]1C=CN=C1 JDIIGWSSTNUWGK-UHFFFAOYSA-N 0.000 description 1
239000002028 Biomass Substances 0.000 description 1
239000004215 Carbon black (E152) Substances 0.000 description 1
102000002812 Heat-Shock Proteins Human genes 0.000 description 1
108010004889 Heat-Shock Proteins Proteins 0.000 description 1
241000238631 Hexapoda Species 0.000 description 1
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
208000035472 Zoonoses Diseases 0.000 description 1
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229940037003 alum Drugs 0.000 description 1
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230000006860 carbon metabolism Effects 0.000 description 1
239000013592 cell lysate Substances 0.000 description 1
230000003833 cell viability Effects 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
239000013522 chelant Substances 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000012364 cultivation method Methods 0.000 description 1
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125000004122 cyclic group Chemical group 0.000 description 1
231100000135 cytotoxicity Toxicity 0.000 description 1
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238000003936 denaturing gel electrophoresis Methods 0.000 description 1
238000000326 densiometry Methods 0.000 description 1
230000001627 detrimental effect Effects 0.000 description 1
238000001035 drying Methods 0.000 description 1
239000012149 elution buffer Substances 0.000 description 1
230000002708 enhancing effect Effects 0.000 description 1
238000011067 equilibration Methods 0.000 description 1
235000020774 essential nutrients Nutrition 0.000 description 1
239000013613 expression plasmid Substances 0.000 description 1
230000004907 flux Effects 0.000 description 1
238000005187 foaming Methods 0.000 description 1
235000012041 food component Nutrition 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
230000034659 glycolysis Effects 0.000 description 1
239000003966 growth inhibitor Substances 0.000 description 1
230000017525 heat dissipation Effects 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
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150000002430 hydrocarbons Chemical class 0.000 description 1
238000011534 incubation Methods 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
230000002458 infectious effect Effects 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
239000002054 inoculum Substances 0.000 description 1
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239000006166 lysate Substances 0.000 description 1
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241000701447 unidentified baculovirus Species 0.000 description 1
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206010048282 zoonosis Diseases 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Medicinal Chemistry (AREA)
General Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Veterinary Medicine (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Chemical & Material Sciences (AREA)
Pharmacology & Pharmacy (AREA)
Chemical Kinetics & Catalysis (AREA)
Animal Behavior & Ethology (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Public Health (AREA)
Molecular Biology (AREA)
Genetics & Genomics (AREA)
Biophysics (AREA)
Gastroenterology & Hepatology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Engineering & Computer Science (AREA)
Communicable Diseases (AREA)
Oncology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Aiming, Guidance, Guns With A Light Source, Armor, Camouflage, And Targets (AREA)
Laminated Bodies (AREA)
Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)

UA20040503327A 2001-11-05 2001-07-12 Process for preparation of protective antigen protein of anthracis with e.coli UA80809C2 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
IN1127DE2001 IN190590B (de)	2001-11-05	2001-11-05
PCT/IN2001/000215 WO2003040179A1 (en)	2001-11-05	2001-12-07	High level constitutive production of anthrax protective antigen

Publications (1)

Publication Number	Publication Date
UA80809C2 true UA80809C2 (en)	2007-11-12

Family

ID=11097131

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
UA20040503327A UA80809C2 (en)	2001-11-05	2001-07-12	Process for preparation of protective antigen protein of anthracis with e.coli

Country Status (15)

Country	Link
US (1)	US7329513B2 (de)
EP (1)	EP1442052B1 (de)
JP (1)	JP2005510215A (de)
CN (1)	CN100347189C (de)
AT (1)	ATE484517T1 (de)
BR (1)	BRPI0117173A2 (de)
CA (1)	CA2465679A1 (de)
DE (1)	DE60143276D1 (de)
IL (1)	IL161725A0 (de)
IN (1)	IN190590B (de)
MX (1)	MXPA04004281A (de)
RU (1)	RU2288272C2 (de)
UA (1)	UA80809C2 (de)
WO (1)	WO2003040179A1 (de)
ZA (1)	ZA200403396B (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
UA80809C2 (en) *	2001-11-05	2007-11-12		Process for preparation of protective antigen protein of anthracis with e.coli
AU2004272972A1 (en)	2003-05-22	2005-03-24	Fraunhofer Usa, Inc.	Recombinant carrier molecule for expression, delivery and purification of target polypeptides
AU2003271835A1 (en) *	2003-06-06	2005-01-04	Avecia Limited	Process for the separation of proteins
US20060246079A1 (en) *	2003-11-14	2006-11-02	Morrow Phillip R	Neutralizing human antibodies to anthrax toxin
WO2005081749A2 (en) *	2004-01-23	2005-09-09	Avanir Pharmaceuticals, Inc.	Neutralizing human antibodies to anthraxtoxin
US7355027B2 (en)	2004-06-16	2008-04-08	Dynport Vaccine Company Llc	Bacillus anthracis protective antigen
US8101735B2 (en)	2004-06-16	2012-01-24	Health Protection Agency	Preparation of protective antigen
CN100336908C (zh) *	2004-09-10	2007-09-12	中国人民解放军军事医学科学院微生物流行病研究所	一种制备重组炭疽保护性抗原的方法及其专用表达质粒
EP1919504B1 (de)	2005-08-03	2013-10-16	iBio, Inc.	Antikörper gegen bacillus anthracis protektiv antigen
US8277816B2 (en) *	2006-02-13	2012-10-02	Fraunhofer Usa, Inc.	Bacillus anthracis antigens, vaccine compositions, and related methods
AU2007215080A1 (en)	2006-02-13	2007-08-23	Fraunhofer Usa, Inc.	Influenza antigens, vaccine compositions, and related methods
GB0607462D0 (en) *	2006-04-13	2006-05-24	Avecia Ltd	Assay
EP2021021A2 (de) *	2006-05-12	2009-02-11	Oklahoma Medical Research Foundation	Anthrax-zusammensetzungen und verfahren zu ihrer herstellung und anwendung
EP2264143A3 (de)	2006-06-30	2012-03-07	University Of Georgia Research Foundation, Inc.	Anthrax-Kohlenhydrate sowie Synthese und Verwendungen davon
US8420607B2 (en)	2006-06-30	2013-04-16	University Of Georgia Research Foundation, Inc.	Anthrax carbohydrates, synthesis and uses thereof
US8404252B2 (en)	2007-07-11	2013-03-26	Fraunhofer Usa, Inc.	Yersinia pestis antigens, vaccine compositions, and related methods
WO2009126355A2 (en) *	2008-01-28	2009-10-15	Baxter International, Inc.	Methods of increasing recombinant production of bacillus anthracis protective antigen
WO2010037046A1 (en)	2008-09-28	2010-04-01	Fraunhofer Usa, Inc.	Humanized neuraminidase antibody and methods of use thereof
JP5701760B2 (ja) *	2008-10-02	2015-04-15	ファーマシーネ，インコーポレイテッド	炭疽菌ワクチン製剤とその用途
US20100183675A1 (en) *	2009-01-22	2010-07-22	Allan Watkinson	Stable vaccine compositions and methods of use
EP2483307A1 (de)	2009-09-29	2012-08-08	Fraunhofer USA, Inc.	Influenza-hämagglutinin-antikörper, zusammensetzungen daraus und zugehörige verfahren
US20120301951A1 (en) *	2010-11-18	2012-11-29	E. I. Du Pont De Nemours And Company	Prevention of contamination of nutrient feed reservoirs & feed lines in bioreactor systems
RU2633508C1 (ru) *	2016-12-27	2017-10-12	Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук (ФИЦ Биотехнологии РАН)	Штамм E. coli - продуцент изолированного домена 1 протективного антигена Bacillus anthracis в форме вирусоподобных частиц
RU2633504C1 (ru) *	2016-12-27	2017-10-12	Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук (ФИЦ Биотехнологии РАН)	Штамм E.coli - продуцент полноразмерного протективного антигена Bacillus anthracis в форме вирусоподобных частиц
CN115125224B (zh) *	2022-08-11	2023-07-18	福州大学	一种可规模化生产具有活性的dna聚合酶的方法
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IN190590B (de)	2003-08-09
IL161725A0 (en)	2005-11-20
HK1072610A1 (zh)	2005-09-02
BRPI0117173A2 (pt)	2018-04-17
CA2465679A1 (en)	2003-05-15
EP1442052A1 (de)	2004-08-04
WO2003040179A1 (en)	2003-05-15
EP1442052B1 (de)	2010-10-13
US20050054038A1 (en)	2005-03-10
DE60143276D1 (de)	2010-11-25
US7329513B2 (en)	2008-02-12
ZA200403396B (en)	2005-08-05
ATE484517T1 (de)	2010-10-15
MXPA04004281A (es)	2005-03-31
CN100347189C (zh)	2007-11-07
CN1582298A (zh)	2005-02-16
RU2288272C2 (ru)	2006-11-27
JP2005510215A (ja)	2005-04-21
RU2004117085A (ru)	2005-03-27

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