WO2000004385A1 - Procede de detection de proteines - Google Patents

Procede de detection de proteines Download PDF

Info

Publication number
WO2000004385A1
WO2000004385A1 PCT/RU1998/000371 RU9800371W WO0004385A1 WO 2000004385 A1 WO2000004385 A1 WO 2000004385A1 RU 9800371 W RU9800371 W RU 9800371W WO 0004385 A1 WO0004385 A1 WO 0004385A1
Authority
WO
WIPO (PCT)
Prior art keywords
membrane
proteins
antibodies
cell
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/RU1998/000371
Other languages
English (en)
Russian (ru)
Inventor
Igor Jurievich Malyshev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU15144/99A priority Critical patent/AU1514499A/en
Publication of WO2000004385A1 publication Critical patent/WO2000004385A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Definitions

  • the invention is related to biology, in particular, to
  • the ligand is facilitated by the integration between the ligand and
  • Antibody on this protein includes 9
  • the claimed result is a non-operational result.
  • squirrels up to 1000 species in one community or one and the same squirrel
  • the membrane is broken into cells, cells
  • membranes apply selected antibodies, immobilize
  • the area of the cellulose membrane is broken up into
  • One membrane can reach 1000 and above.
  • the cell is marked with digital and alphanumeric
  • the antibody in one cell or, depending on the task, all
  • the antibody is »3 mm. After this, they exist
  • the liver of the liver in the liver was used.
  • the claimed method is significantly larger.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Cette invention se rapporte au domaine de la biologie, plus précisément, à celui de la biochimie et de la biologie moléculaire, et peut être utilisée en médecine expérimentale et clinique. Cette invention permet de détecter simultanément un grand nombre de protéines différentes (jusqu'à 1000 dénominations) dans un seul dosage ou, encore, de détecter une seule et même protéine dans un grand nombre de dosages. A cette fin, on utilise une membrane de nitrocellulose que l'on divise en cellules (jusqu'à 1000) d'une taille de 3 à 5 mm, lesdites cellules étant répertoriées à l'aide de deux coordonnées. On ajoute ensuite des anticorps prédéterminés au milieu de chaque cellule de la membrane, on immobilise ces anticorps et, après rinçage de la membrane, on applique sur cette dernière des protéines préalablement étiquetées dans un dosage. On laisse ensuite reposer pendant 1 à 2 heures et l'on rince la membrane afin d'éliminer les excédents. La teneur en protéines est déterminée selon un procédé radiographique, tandis que l'identification des protéines se fait en fonction du numéro de la cellule (de l'anticorps).
PCT/RU1998/000371 1998-07-17 1998-11-12 Procede de detection de proteines Ceased WO2000004385A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU15144/99A AU1514499A (en) 1998-07-17 1998-11-12 Method for detecting proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU98113214 1998-07-17
RU98113214A RU2167425C2 (ru) 1998-07-17 1998-07-17 Способ определения белков

Publications (1)

Publication Number Publication Date
WO2000004385A1 true WO2000004385A1 (fr) 2000-01-27

Family

ID=20208255

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU1998/000371 Ceased WO2000004385A1 (fr) 1998-07-17 1998-11-12 Procede de detection de proteines

Country Status (3)

Country Link
AU (1) AU1514499A (fr)
RU (1) RU2167425C2 (fr)
WO (1) WO2000004385A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2018107453A (ru) 2015-08-10 2019-09-12 Эссенликс Корп. Устройства для анализов и способы в биологии/химии, предусматривающие упрощенные стадии, образцы небольшого объема, повышенную скорость и простоту применения
MY194887A (en) 2015-09-14 2022-12-21 Essenlix Corp Device and System for Analyzing a Sample, Particularly Blood, as Well as Methods of Using The Same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1603301A1 (ru) * 1987-12-22 1990-10-30 Карагандинский государственный медицинский институт Способ определени концентрации иммуноглобулинов в биологической жидкости
RU2038600C1 (ru) * 1990-07-31 1995-06-27 Институт иммунобиотехнологии Мембрана для разделения компонентов иммунологической реакции
WO1995025543A1 (fr) * 1994-03-18 1995-09-28 The Scripps Research Institute Compositions inhibitrices de l'angiogenese et procedes correspondants
RU2095815C1 (ru) * 1994-12-22 1997-11-10 Леонид Николаевич Падюков Способ получения иммуносорбента для диагностики вирусного гепатита с

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1603301A1 (ru) * 1987-12-22 1990-10-30 Карагандинский государственный медицинский институт Способ определени концентрации иммуноглобулинов в биологической жидкости
RU2038600C1 (ru) * 1990-07-31 1995-06-27 Институт иммунобиотехнологии Мембрана для разделения компонентов иммунологической реакции
WO1995025543A1 (fr) * 1994-03-18 1995-09-28 The Scripps Research Institute Compositions inhibitrices de l'angiogenese et procedes correspondants
RU2095815C1 (ru) * 1994-12-22 1997-11-10 Леонид Николаевич Падюков Способ получения иммуносорбента для диагностики вирусного гепатита с

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHARD T., Radio immunologicheskie metody, under editorship, Ya. M, Varshavskoho, Moscow, iizd. (MIR), 1988, page 126. *
KRUSTALEVA N.A., "Vydelenie, ochistka, nekotorye fizikokhimicheskie svoystva leitsinaminopeptidazy mielina i diagnosticheskoe zhachmie etogo fermenta pri demielinizirujuschikh zobolevanyakh", Avtoreferat dissertatsii Moscow, Universitet druzhby narodov, 1987, pages 6-7, 10-11. *

Also Published As

Publication number Publication date
RU2167425C2 (ru) 2001-05-20
AU1514499A (en) 2000-02-07

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