WO2003038082A3 - Alkalische protease-varianten und wash- und reinigungsmittel enthaltend diese alkalischen protease-varianten - Google Patents

Alkalische protease-varianten und wash- und reinigungsmittel enthaltend diese alkalischen protease-varianten Download PDF

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Publication number
WO2003038082A3
WO2003038082A3 PCT/EP2002/011725 EP0211725W WO03038082A3 WO 2003038082 A3 WO2003038082 A3 WO 2003038082A3 EP 0211725 W EP0211725 W EP 0211725W WO 03038082 A3 WO03038082 A3 WO 03038082A3
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WO
WIPO (PCT)
Prior art keywords
alkaline protease
protease variants
novel alkaline
variants
detergents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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PCT/EP2002/011725
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English (en)
French (fr)
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WO2003038082A2 (de
Inventor
Beatrix Kottwitz
Karl-Heinz Maurer
Roland Breves
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Henkel AG and Co KGaA
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Henkel AG and Co KGaA
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Application filed by Henkel AG and Co KGaA filed Critical Henkel AG and Co KGaA
Priority to HK05102333.7A priority Critical patent/HK1069849B/xx
Priority to JP2003540347A priority patent/JP2005507259A/ja
Priority to HU0500058A priority patent/HU225362B1/hu
Priority to EP02785253A priority patent/EP1442120A2/de
Publication of WO2003038082A2 publication Critical patent/WO2003038082A2/de
Publication of WO2003038082A3 publication Critical patent/WO2003038082A3/de
Priority to US10/836,959 priority patent/US7320887B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Fodder In General (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Treatment And Processing Of Natural Fur Or Leather (AREA)
  • Dairy Products (AREA)

Abstract

Gegenstand der vorliegenden Anmeldung sind neue Alkalische Protease-Varianten. Diese weisen in der Zählung der Alkalischen Protease aus Bacillus lentus Variationen in der Aminosäure-Position 61, den Positionen 199 und/oder 211 and optional mindestens eine zur Stabilisierung des Moleküls beitragende Modifizierung, vorzugsweise Punktmutationen in den Positionen 3 and/oder 4 auf. Besonders bevorzugt sind die Varianten S3TN4l/G61A/V199l und S3T/V4l/G61A/V199l/L211D der B.lentus-Alkalischen Protease. Die vorliegende Erfindung betrifft auch die Einsatzmöglichkeiten dieser Enzyme in diversen technischen Prozessen and insbesondere Wasch- und Reinigungsmittel mit diesen neuen Alkalischen Protease-Varianten.
PCT/EP2002/011725 2001-10-31 2002-10-19 Alkalische protease-varianten und wash- und reinigungsmittel enthaltend diese alkalischen protease-varianten Ceased WO2003038082A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
HK05102333.7A HK1069849B (en) 2001-10-31 2002-10-19 Novel alkaline protease variants and detergents and cleansers containing these novel alkaline protease variants
JP2003540347A JP2005507259A (ja) 2001-10-31 2002-10-19 新規なアルカリ性プロテアーゼ変異体ならびに該新規なアルカリ性プロテアーゼ変異体を含有する洗浄剤および浄化剤
HU0500058A HU225362B1 (en) 2001-10-31 2002-10-19 Novel alkaline protease variants and detergents and cleansers containing these novel alkaline protease variants
EP02785253A EP1442120A2 (de) 2001-10-31 2002-10-19 Alkalische protease-varianten und wasch- und reinigungsmittel enthaltend diese alkalischen protease-varianten
US10/836,959 US7320887B2 (en) 2001-10-31 2004-04-30 Alkaline protease variants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10153792.1 2001-10-31
DE10153792A DE10153792A1 (de) 2001-10-31 2001-10-31 Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/836,959 Continuation US7320887B2 (en) 2001-10-31 2004-04-30 Alkaline protease variants

Publications (2)

Publication Number Publication Date
WO2003038082A2 WO2003038082A2 (de) 2003-05-08
WO2003038082A3 true WO2003038082A3 (de) 2003-09-18

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/011725 Ceased WO2003038082A2 (de) 2001-10-31 2002-10-19 Alkalische protease-varianten und wash- und reinigungsmittel enthaltend diese alkalischen protease-varianten

Country Status (7)

Country Link
US (1) US7320887B2 (de)
EP (1) EP1442120A2 (de)
JP (1) JP2005507259A (de)
CN (1) CN100462434C (de)
DE (1) DE10153792A1 (de)
HU (1) HU225362B1 (de)
WO (1) WO2003038082A2 (de)

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