WO2009010841A3 - Culture d'expansion de cellules souches ex vivo - Google Patents

Culture d'expansion de cellules souches ex vivo Download PDF

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Publication number
WO2009010841A3
WO2009010841A3 PCT/IB2008/001798 IB2008001798W WO2009010841A3 WO 2009010841 A3 WO2009010841 A3 WO 2009010841A3 IB 2008001798 W IB2008001798 W IB 2008001798W WO 2009010841 A3 WO2009010841 A3 WO 2009010841A3
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WIPO (PCT)
Prior art keywords
culture
vivo
cells
stem cells
expanding stem
Prior art date
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Ceased
Application number
PCT/IB2008/001798
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English (en)
Other versions
WO2009010841A2 (fr
WO2009010841A8 (fr
Inventor
Lorenza Lazzari
Tiziana Montemurro
Rosaria Giordano
Paolo Rebulla
Girolamo Sirchia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FONDAZIONE IRCCS ITITUTO DI RICOVERO E CURA A CARATTERE SCIENTIFICO DI NATURA PUBBLICA OSPEDALE MAGGIORE POLICLINICO MANGIAGALLI E REGINA ELENA
FOND IRCCS ITITUTO DI RICOVERO
Original Assignee
FONDAZIONE IRCCS ITITUTO DI RICOVERO E CURA A CARATTERE SCIENTIFICO DI NATURA PUBBLICA OSPEDALE MAGGIORE POLICLINICO MANGIAGALLI E REGINA ELENA
FOND IRCCS ITITUTO DI RICOVERO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by FONDAZIONE IRCCS ITITUTO DI RICOVERO E CURA A CARATTERE SCIENTIFICO DI NATURA PUBBLICA OSPEDALE MAGGIORE POLICLINICO MANGIAGALLI E REGINA ELENA, FOND IRCCS ITITUTO DI RICOVERO filed Critical FONDAZIONE IRCCS ITITUTO DI RICOVERO E CURA A CARATTERE SCIENTIFICO DI NATURA PUBBLICA OSPEDALE MAGGIORE POLICLINICO MANGIAGALLI E REGINA ELENA
Priority to JP2010516605A priority Critical patent/JP2010533486A/ja
Priority to EP08788872A priority patent/EP2167646A2/fr
Priority to AU2008277391A priority patent/AU2008277391A1/en
Priority to CA2693420A priority patent/CA2693420A1/fr
Priority to MX2010000496A priority patent/MX2010000496A/es
Priority to US12/668,818 priority patent/US9206389B2/en
Priority to BRPI0814082-0A2A priority patent/BRPI0814082A2/pt
Priority to CN200880024832A priority patent/CN101743305A/zh
Publication of WO2009010841A2 publication Critical patent/WO2009010841A2/fr
Publication of WO2009010841A3 publication Critical patent/WO2009010841A3/fr
Publication of WO2009010841A8 publication Critical patent/WO2009010841A8/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/145—Thrombopoietin [TPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Gynecology & Obstetrics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Rheumatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Oncology (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne une culture contenant un milieu de croissance et une combinaison de cytokines constituée i) d'interleukine-6 (IL6) ; ii) de ligand Flt3 (FLT3) ; iii) de facteur des cellules souches (FCS) ; et iv) de thrombopoïétine (TPO). L'invention concerne également l'utilisation de cette culture dans l'expansion ex vivo de cellules souches et/ou de cellules parentales et de cellules différenciées à partir de celles-ci, ainsi que l'utilisation des cellules obtenues à partir de cette expansion.
PCT/IB2008/001798 2007-07-16 2008-07-09 Culture d'expansion de cellules souches ex vivo Ceased WO2009010841A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP2010516605A JP2010533486A (ja) 2007-07-16 2008-07-09 幹細胞の生体外増殖培地
EP08788872A EP2167646A2 (fr) 2007-07-16 2008-07-09 Culture d'expansion de cellules souches ex vivo
AU2008277391A AU2008277391A1 (en) 2007-07-16 2008-07-09 Culture for expanding stem cells ex-vivo
CA2693420A CA2693420A1 (fr) 2007-07-16 2008-07-09 Culture d'expansion de cellules souches ex vivo
MX2010000496A MX2010000496A (es) 2007-07-16 2008-07-09 Cultivo para expandir citoblastos ex-vivo.
US12/668,818 US9206389B2 (en) 2007-07-16 2008-07-09 Culture for expanding stem cells ex-vivo
BRPI0814082-0A2A BRPI0814082A2 (pt) 2007-07-16 2008-07-09 Cultura incluindo um meio de expansão e uma composição de citocinas, método para a expansão de células-tronco, células-tronco e uso de células
CN200880024832A CN101743305A (zh) 2007-07-16 2008-07-09 离体扩增干细胞的培养物

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT001421A ITMI20071421A1 (it) 2007-07-16 2007-07-16 Coltura per espandere cellule staminali ex-vivo
ITMI2007A001421 2007-07-16

Publications (3)

Publication Number Publication Date
WO2009010841A2 WO2009010841A2 (fr) 2009-01-22
WO2009010841A3 true WO2009010841A3 (fr) 2009-03-12
WO2009010841A8 WO2009010841A8 (fr) 2009-05-14

Family

ID=40202037

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/001798 Ceased WO2009010841A2 (fr) 2007-07-16 2008-07-09 Culture d'expansion de cellules souches ex vivo

Country Status (12)

Country Link
US (1) US9206389B2 (fr)
EP (1) EP2167646A2 (fr)
JP (1) JP2010533486A (fr)
KR (1) KR20100040311A (fr)
CN (1) CN101743305A (fr)
AU (1) AU2008277391A1 (fr)
BR (1) BRPI0814082A2 (fr)
CA (1) CA2693420A1 (fr)
IT (1) ITMI20071421A1 (fr)
MX (1) MX2010000496A (fr)
RU (1) RU2010105048A (fr)
WO (1) WO2009010841A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10384207B2 (en) 2015-07-21 2019-08-20 Neuro Probe Incorporated Assay apparatus and methods
ES2862676T3 (es) 2015-10-20 2021-10-07 Fujifilm Cellular Dynamics Inc Producción de células precursoras hematopoyéticas multilinaje mediante programación genética
EP3523422B1 (fr) 2016-10-05 2024-12-25 FUJIFILM Cellular Dynamics, Inc. Génération de lignées matures à partir de cellules souches pluripotentes induites avec une interruption mecp2
CN106754712B (zh) * 2016-11-21 2020-10-16 中国人民解放军军事医学科学院野战输血研究所 诱导脐带血单个核细胞分化为粒系细胞的方法
AU2018254442B2 (en) 2017-04-18 2024-03-28 FUJIFILM Cellular Dynamics, Inc. Antigen-specific immune effector cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005007799A2 (fr) * 2003-07-17 2005-01-27 Gamida-Cell Ltd. Procedes de multiplication ex-vivo de cellules souches / progenitrices

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2882992A (en) * 1991-11-06 1993-06-07 Arthur A. Axelrad Cell culture medium
JP2002529071A (ja) * 1998-11-09 2002-09-10 コンソルツィオ・ペール・ラ・ジェスティオーネ・デル・チェントロ・ディ・ビオテクノロジー・アヴァンツァテ 軟骨細胞様細胞のための無血清培地

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005007799A2 (fr) * 2003-07-17 2005-01-27 Gamida-Cell Ltd. Procedes de multiplication ex-vivo de cellules souches / progenitrices

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRUNO S ET AL: "Different growth factor requirements for the ex vivo amplification of transplantable human cord blood cells in a NOD/SCID mouse model", JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS, vol. 15, no. 1, January 2001 (2001-01-01), pages 38 - 48, XP009110635, ISSN: 0393-974X *
ENCABO ARACELI ET AL: "Selective generation of different dendritic cell precursors from CD34+ cells by interleukin-6 and interleukin-3", STEM CELLS (MIAMISBURG), vol. 22, no. 5, 2004, pages 725 - 740, XP002510073, ISSN: 1066-5099 *
MURRAY LESLEY J ET AL: "Thrombopoietin, flt3, and kit ligands together suppress apoptosis of human mobilized CD34+ cells and recruit primitive CD34+Thy-1+ cells into rapid division", EXPERIMENTAL HEMATOLOGY (CHARLOTTESVILLE), vol. 27, no. 6, June 1999 (1999-06-01), pages 1019 - 1028, XP002510074, ISSN: 0301-472X *
PELED TONI ET AL: "Linear polyamine copper chelator tetraethylenepentamine augments long-term ex vivo expansion of cord blood-derived CD34+ cells and increases their engraftment potential in NOD/SCID mice", EXPERIMENTAL HEMATOLOGY (NEW YORK), vol. 32, no. 6, June 2004 (2004-06-01), pages 547 - 555, XP002510075, ISSN: 0301-472X *
SANTONI DE SIO ET AL: "1038. Lentiviral Gene Transfer into HSC Is Enhanced by Early-Acting Cytokines without Impairing Stem Cell Properties and Involves Cellular Responses Distinct from Cell Cycle Control", MOLECULAR THERAPY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 11, 15 August 2005 (2005-08-15), pages 401, XP005016377, ISSN: 1525-0016 *

Also Published As

Publication number Publication date
MX2010000496A (es) 2010-04-27
BRPI0814082A2 (pt) 2014-10-07
KR20100040311A (ko) 2010-04-19
CN101743305A (zh) 2010-06-16
US20100189700A1 (en) 2010-07-29
US9206389B2 (en) 2015-12-08
WO2009010841A2 (fr) 2009-01-22
EP2167646A2 (fr) 2010-03-31
RU2010105048A (ru) 2011-08-27
ITMI20071421A1 (it) 2009-01-17
CA2693420A1 (fr) 2009-01-22
AU2008277391A1 (en) 2009-01-22
JP2010533486A (ja) 2010-10-28
WO2009010841A8 (fr) 2009-05-14

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