WO2013149729A3 - Proinsuline comprenant une séquence auxiliaire améliorée - Google Patents

Proinsuline comprenant une séquence auxiliaire améliorée Download PDF

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Publication number
WO2013149729A3
WO2013149729A3 PCT/EP2013/001003 EP2013001003W WO2013149729A3 WO 2013149729 A3 WO2013149729 A3 WO 2013149729A3 EP 2013001003 W EP2013001003 W EP 2013001003W WO 2013149729 A3 WO2013149729 A3 WO 2013149729A3
Authority
WO
WIPO (PCT)
Prior art keywords
insulin
proinsulin
helper sequence
organism
precursor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2013/001003
Other languages
English (en)
Other versions
WO2013149729A2 (fr
Inventor
Kelvin Eckert
Stefan Hermann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GLUCOMETRIX AG
Original Assignee
GLUCOMETRIX AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GLUCOMETRIX AG filed Critical GLUCOMETRIX AG
Priority to EP13737127.4A priority Critical patent/EP2867250A2/fr
Publication of WO2013149729A2 publication Critical patent/WO2013149729A2/fr
Publication of WO2013149729A3 publication Critical patent/WO2013149729A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un polypeptide comprenant un précurseur d'insuline et la séquence auxiliaire N-terminale MAKR. La séquence auxiliaire améliore l'expression du précurseur d'insuline dans un organisme recombiné et augmente les rendements en insuline après purification et traitement du précurseur d'insuline. L'invention concerne en outre un procédé de production d'insuline, dans lequel la proinsuline est exprimée comme polypeptide recombiné dans un organisme tel qu'E. coli, isolé de l'organisme puis traité pour donner l'insuline active.
PCT/EP2013/001003 2012-04-04 2013-04-04 Proinsuline comprenant une séquence auxiliaire améliorée Ceased WO2013149729A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP13737127.4A EP2867250A2 (fr) 2012-04-04 2013-04-04 Proinsuline comprenant une séquence auxiliaire améliorée

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12002446.8 2012-04-04
EP12002446 2012-04-04

Publications (2)

Publication Number Publication Date
WO2013149729A2 WO2013149729A2 (fr) 2013-10-10
WO2013149729A3 true WO2013149729A3 (fr) 2013-12-05

Family

ID=48793151

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/001003 Ceased WO2013149729A2 (fr) 2012-04-04 2013-04-04 Proinsuline comprenant une séquence auxiliaire améliorée

Country Status (2)

Country Link
EP (1) EP2867250A2 (fr)
WO (1) WO2013149729A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170036643A (ko) * 2015-09-24 2017-04-03 한미약품 주식회사 인슐린의 제조 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0534705A2 (fr) * 1991-09-24 1993-03-31 Eli Lilly And Company Expression de polypeptides recombinantes à bas poids moléculaires
WO2001018215A1 (fr) * 1999-09-09 2001-03-15 Max-Planck-Gesellschaft E.V. Procede de fabrication de serine-proteases actives et de variants inactifs
WO2012048856A1 (fr) * 2010-10-12 2012-04-19 Glucometrix Pvs Gmbh Proinsuline avec séquence auxiliaire

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3837271A1 (de) 1988-11-03 1990-05-10 Hoechst Ag Verfahren zur selektiven spaltung von fusionsproteinen
BR9408638A (pt) 1994-12-29 2004-09-21 Bio Technology General Corp Geração de insulina humana

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0534705A2 (fr) * 1991-09-24 1993-03-31 Eli Lilly And Company Expression de polypeptides recombinantes à bas poids moléculaires
WO2001018215A1 (fr) * 1999-09-09 2001-03-15 Max-Planck-Gesellschaft E.V. Procede de fabrication de serine-proteases actives et de variants inactifs
WO2012048856A1 (fr) * 2010-10-12 2012-04-19 Glucometrix Pvs Gmbh Proinsuline avec séquence auxiliaire

Also Published As

Publication number Publication date
EP2867250A2 (fr) 2015-05-06
WO2013149729A2 (fr) 2013-10-10

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