ZA200202155B - Leptin assay. - Google Patents

Leptin assay. Download PDF

Info

Publication number: ZA200202155B
Authority: ZA; South Africa
Prior art keywords: leptin; ang; cell; cell line; promoter
Prior art date: 1999-10-11

Application number

ZA200202155A

Other languages

English (en)

Inventor

Menachem Rubenstein

Batya Cohen

Original Assignee

Yeda Res & Dev

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-10-11

Filing date

2002-03-15

Publication date

2003-05-28

2002-03-15 Application filed by Yeda Res & Dev filed Critical Yeda Res & Dev

2003-05-28 Publication of ZA200202155B publication Critical patent/ZA200202155B/en

Links

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Classifications

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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- C—CHEMISTRY; METALLURGY
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/136—Screening for pharmacological compounds
- C—CHEMISTRY; METALLURGY
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Investigating Or Analysing Biological Materials (AREA)
Peptides Or Proteins (AREA)

ZA200202155A 1999-10-11 2002-03-15 Leptin assay. ZA200202155B (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
IL13231399A IL132313A0 (en)	1999-10-11	1999-10-11	Leptin assay

Publications (1)

Publication Number	Publication Date
ZA200202155B true ZA200202155B (en)	2003-05-28

Family

ID=11073317

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ZA200202155A ZA200202155B (en)	1999-10-11	2002-03-15	Leptin assay.

Country Status (24)

Country	Link
US (1)	US7247427B1 (da)
EP (1)	EP1222467B1 (da)
JP (1)	JP2003511057A (da)
KR (1)	KR100863580B1 (da)
CN (1)	CN1159585C (da)
AR (1)	AR025995A1 (da)
AT (1)	ATE322017T1 (da)
AU (1)	AU778727B2 (da)
BR (1)	BR0014626A (da)
CA (1)	CA2386365A1 (da)
CY (1)	CY1105025T1 (da)
DE (1)	DE60027031T2 (da)
DK (1)	DK1222467T3 (da)
EA (1)	EA004709B1 (da)
EE (1)	EE200200189A (da)
ES (1)	ES2256045T3 (da)
HK (1)	HK1048514B (da)
IL (2)	IL132313A0 (da)
NO (1)	NO20021604L (da)
PT (1)	PT1222467E (da)
SI (1)	SI1222467T1 (da)
UA (1)	UA77153C2 (da)
WO (1)	WO2001027319A2 (da)
ZA (1)	ZA200202155B (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP2372364A1 (en) *	2010-03-31	2011-10-05	Medizinische Hochschule Hannover	Biomarker for pulmonary hypertension
EP2661266B1 (en)	2011-01-07	2020-09-16	Anji Pharma (US) LLC	Chemosensory receptor ligand-based therapies
US9795792B2 (en)	2011-02-25	2017-10-24	Medtronic, Inc.	Emergency mode switching for non-pacing modes
CN102221615A (zh) *	2011-03-31	2011-10-19	广州华灿医药科技有限公司	一种基于Angiogenin检测的双抗夹心ELISA方法
KR20190120430A (ko)	2012-01-06	2019-10-23	엘셀릭스 테라퓨틱스 인코포레이티드	바이구아나이드 조성물 및 대사 장애를 치료하는 방법
EP2800562A2 (en)	2012-01-06	2014-11-12	Elcelyx Therapeutics, Inc.	Compositions and methods for treating metabolic disorders
WO2013158928A2 (en)	2012-04-18	2013-10-24	Elcelyx Therapeutics, Inc.	Chemosensory receptor ligand-based therapies
HK1210825A1 (en)	2012-07-11	2016-05-06	Elcelyx Therapeutics, Inc.	Compositions comprising statins, biguanides and further agents for reducing cardiometabolic risk
CN102901823A (zh) *	2012-10-26	2013-01-30	安徽安科生物工程（集团）股份有限公司	瘦素检测试剂盒
CN105101956B (zh)	2013-01-05	2018-12-07	埃尔舍利克斯治疗公司	包含双胍的延迟释放组合物
CN104459105B (zh) *	2014-12-05	2016-03-09	重庆中元生物技术有限公司	一种检测lp的胶乳增强免疫比浊试剂盒及其制备方法
CN115901697B (zh) *	2021-08-26	2026-03-17	苏州生物医药转化工程中心	一种检测瘦素相关蛋白或其类似物的活性的方法
CN114940717B (zh) *	2022-06-30	2024-04-26	武汉海特生物制药股份有限公司	嵌合受体、32d瘦素敏感细胞株及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0914611A4 (en)	1996-04-22	2002-10-16	Merck & Co Inc	TRANSACTIVATION OF LEPTIN

1999
- 1999-10-11 IL IL13231399A patent/IL132313A0/xx unknown
2000
- 2000-05-10 UA UA2002053825A patent/UA77153C2/uk unknown
- 2000-10-05 CN CNB008140359A patent/CN1159585C/zh not_active Expired - Fee Related
- 2000-10-05 US US10/110,243 patent/US7247427B1/en not_active Expired - Fee Related
- 2000-10-05 CA CA002386365A patent/CA2386365A1/en not_active Abandoned
- 2000-10-05 KR KR1020027003765A patent/KR100863580B1/ko not_active Expired - Fee Related
- 2000-10-05 JP JP2001529450A patent/JP2003511057A/ja active Pending
- 2000-10-05 PT PT00968194T patent/PT1222467E/pt unknown
- 2000-10-05 EP EP00968194A patent/EP1222467B1/en not_active Expired - Lifetime
- 2000-10-05 DE DE60027031T patent/DE60027031T2/de not_active Expired - Fee Related
- 2000-10-05 EE EEP200200189A patent/EE200200189A/xx unknown
- 2000-10-05 EA EA200200435A patent/EA004709B1/ru not_active IP Right Cessation
- 2000-10-05 DK DK00968194T patent/DK1222467T3/da active
- 2000-10-05 SI SI200030841T patent/SI1222467T1/sl unknown
- 2000-10-05 ES ES00968194T patent/ES2256045T3/es not_active Expired - Lifetime
- 2000-10-05 HK HK03100201.2A patent/HK1048514B/zh not_active IP Right Cessation
- 2000-10-05 AT AT00968194T patent/ATE322017T1/de not_active IP Right Cessation
- 2000-10-05 AU AU78141/00A patent/AU778727B2/en not_active Ceased
- 2000-10-05 WO PCT/IL2000/000621 patent/WO2001027319A2/en not_active Ceased
- 2000-10-05 BR BR0014626-9A patent/BR0014626A/pt not_active Application Discontinuation
- 2000-10-05 IL IL149021A patent/IL149021A/en not_active IP Right Cessation
- 2000-10-09 AR ARP000105316A patent/AR025995A1/es active IP Right Grant
2002
- 2002-03-15 ZA ZA200202155A patent/ZA200202155B/en unknown
- 2002-04-04 NO NO20021604A patent/NO20021604L/no not_active Application Discontinuation
2006
- 2006-05-04 CY CY20061100582T patent/CY1105025T1/el unknown

Also Published As

Publication number	Publication date
IL132313A0 (en)	2001-03-19
IL149021A0 (en)	2002-11-10
SI1222467T1 (sl)	2006-06-30
KR100863580B1 (ko)	2008-10-15
CA2386365A1 (en)	2001-04-19
JP2003511057A (ja)	2003-03-25
US7247427B1 (en)	2007-07-24
PT1222467E (pt)	2006-06-30
UA77153C2 (en)	2006-11-15
AU7814100A (en)	2001-04-23
NO20021604D0 (no)	2002-04-04
AR025995A1 (es)	2002-12-26
ES2256045T3 (es)	2006-07-16
WO2001027319A2 (en)	2001-04-19
DE60027031T2 (de)	2006-08-24
CN1378648A (zh)	2002-11-06
HK1048514A1 (en)	2003-04-04
AU778727B2 (en)	2004-12-16
DK1222467T3 (da)	2006-08-07
EA004709B1 (ru)	2004-06-24
HK1048514B (zh)	2004-12-31
CN1159585C (zh)	2004-07-28
DE60027031D1 (de)	2006-05-18
WO2001027319A3 (en)	2001-06-21
CY1105025T1 (el)	2010-03-03
IL149021A (en)	2007-12-03
EP1222467A2 (en)	2002-07-17
ATE322017T1 (de)	2006-04-15
EE200200189A (et)	2003-06-16
EP1222467B1 (en)	2006-03-29
NO20021604L (no)	2002-04-04
BR0014626A (pt)	2002-06-11
EA200200435A1 (ru)	2002-10-31
KR20020048416A (ko)	2002-06-22

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