ZA919932B - A method of constructing synthetic leader sequences - Google Patents

A method of constructing synthetic leader sequences

Info

Publication number
ZA919932B
ZA919932B ZA919932A ZA919932A ZA919932B ZA 919932 B ZA919932 B ZA 919932B ZA 919932 A ZA919932 A ZA 919932A ZA 919932 A ZA919932 A ZA 919932A ZA 919932 B ZA919932 B ZA 919932B
Authority
ZA
South Africa
Prior art keywords
leader sequences
synthetic leader
constructing synthetic
constructing
sequences
Prior art date
Application number
ZA919932A
Other languages
English (en)
Inventor
Lars Christiansen
Christiansen Lars
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of ZA919932B publication Critical patent/ZA919932B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Consolidation Of Soil By Introduction Of Solidifying Substances Into Soil (AREA)
  • Prevention Of Electric Corrosion (AREA)
  • Bridges Or Land Bridges (AREA)
  • Building Environments (AREA)
  • Working Measures On Existing Buildindgs (AREA)
ZA919932A 1990-12-19 1991-12-18 A method of constructing synthetic leader sequences ZA919932B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK300090A DK300090D0 (da) 1990-12-19 1990-12-19 Fremgangsmaade til fremstilling af leadersekvenser

Publications (1)

Publication Number Publication Date
ZA919932B true ZA919932B (en) 1992-08-26

Family

ID=8118017

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA919932A ZA919932B (en) 1990-12-19 1991-12-18 A method of constructing synthetic leader sequences

Country Status (17)

Country Link
EP (1) EP0563175A1 (da)
JP (1) JPH06503957A (da)
KR (1) KR930703450A (da)
AU (1) AU660161B2 (da)
CA (1) CA2098731A1 (da)
CZ (1) CZ119293A3 (da)
DK (1) DK300090D0 (da)
FI (1) FI932831A0 (da)
HU (1) HUT68751A (da)
IE (1) IE914433A1 (da)
IL (1) IL100408A0 (da)
MX (1) MX9102684A (da)
NZ (1) NZ241011A (da)
PT (1) PT99848A (da)
SK (1) SK62593A3 (da)
WO (1) WO1992011378A1 (da)
ZA (1) ZA919932B (da)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI92601C (fi) * 1992-03-11 1994-12-12 Marja Makarow Menetelmä hyötyproteiinien erittämiseksi hiivoista
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide
US5639642A (en) * 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
IL114160A (en) * 1994-06-17 2006-12-31 Novo Nordisk As Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast
HRP940432B1 (en) * 1994-08-05 2003-10-31 Pliva Pharm & Chem Works Dna sequences encoding biosynthetic insulin precursors and process for preparation of insulin
CN1182451A (zh) 1995-03-17 1998-05-20 诺沃挪第克公司 新的内切葡聚糖酶
DK1975177T3 (da) 1996-03-01 2011-07-25 Novo Nordisk As Appetitundertrykkende peptid, formuleringer dermed og anvendelse deraf
WO1998026080A1 (en) 1996-12-13 1998-06-18 Chiron Corporation Method for expression of heterologous proteins in yeast
WO1999037793A1 (en) 1998-01-23 1999-07-29 Novo Nordisk A/S Process for making desired polypeptides in yeast
CA2392844A1 (en) 1999-12-29 2001-07-12 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
AT410217B (de) * 2000-06-15 2003-03-25 Cistem Biotechnologies Gmbh Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen
IL157842A0 (en) 2001-03-22 2004-03-28 Novo Nordisk Healthcare Ag Coagulation factor vii derivatives
ATE532858T1 (de) 2001-09-27 2011-11-15 Novo Nordisk Healthcare Ag Menschliche gerinnungsfaktor-vii-polypeptide
EP2392655A3 (en) 2003-09-09 2012-02-29 Novo Nordisk Health Care AG Coagulation factor VII polypeptides
EP1687428A1 (en) 2003-11-14 2006-08-09 Novo Nordisk A/S Processes for making acylated insulin
JP5697831B2 (ja) 2003-12-03 2015-04-08 ノヴォ ノルディスク アー／エス 単鎖インシュリン
WO2005078116A1 (fr) * 2004-01-16 2005-08-25 Qiuyun Liu Methode permettant d'isoler de peptides antibacteriens et peptides isoles
MX2007012887A (es) 2005-04-18 2007-12-10 Novo Nordisk As Variantes il-21.
CN102660614A (zh) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 制备成熟胰岛素多肽的方法
WO2007031559A2 (en) 2005-09-14 2007-03-22 Novo Nordisk Health Care Ag Human coagulation factor vii polypeptides
JP5202338B2 (ja) 2006-02-27 2013-06-05 ノボ・ノルデイスク・エー／エス 新規インシュリン誘導体
US8518668B2 (en) 2006-09-27 2013-08-27 Novo Nordisk A/S Method for making maturated insulin polypeptides in a fungal cell
CN101778862B (zh) 2007-08-13 2014-12-17 诺沃-诺迪斯克有限公司 快速作用的胰岛素类似物
EP2178910B1 (en) 2007-08-15 2014-10-08 Novo Nordisk A/S Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids
ES2548304T3 (es) 2007-08-15 2015-10-15 Novo Nordisk A/S Análogos de la insulina que contienen una fracción acilo y alquilenglicol
WO2011089170A2 (en) 2010-01-22 2011-07-28 Novo Nordisk A/S Process for preparing fgf-21 with low degree of o-glycosylation
WO2010103038A1 (en) 2009-03-11 2010-09-16 Novo Nordisk A/S Interleukin-21 variants having antagonistic binding to the il-21 receptor
WO2011006982A2 (en) 2009-07-17 2011-01-20 Rigshospitalet Inhibitors of complement activation
EP2504355B1 (en) 2009-11-25 2015-07-22 Novo Nordisk A/S Method for making polypeptides
US9096843B2 (en) 2009-12-01 2015-08-04 Novo Nordisk A/S Peptidyl α-hydroxyglycine α-amidating lyases
US9815876B2 (en) 2010-03-05 2017-11-14 Omeros Corporation Chimeric inhibitor molecules of complement activation
US9074015B2 (en) * 2010-07-28 2015-07-07 Smartcells, Inc. Recombinantly expressed insulin polypeptides and uses thereof
JP6517018B2 (ja) 2012-02-09 2019-05-22 ヴァー２・ファーマシューティカルズ・アンパルトセルスカブＶＡＲ２ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ ＡｐＳ コンドロイチン硫酸グリカンのターゲティング
JP2015532307A (ja) 2012-10-15 2015-11-09 ノヴォ・ノルディスク・ヘルス・ケア・アーゲー 凝固因子ｖｉｉポリペプチド
US20160115216A1 (en) 2013-06-07 2016-04-28 Novo Nordisk A/S Method for Making Mature Insulin Polypeptides
CN106029688A (zh) * 2014-02-28 2016-10-12 诺和诺德股份有限公司 交配因子α原肽变体
US20210278406A1 (en) 2018-07-13 2021-09-09 Varct Diagnostic Aps Isolation of circulating cells of fetal origin using recombinant malaria protein var2csa

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK463887D0 (da) * 1987-09-07 1987-09-07 Novo Industri As Gaerleader
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
AU633020B2 (en) * 1988-07-23 1993-01-21 Novozymes Delta Limited New secretory leader sequences
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid

Also Published As

Publication number Publication date
WO1992011378A1 (en) 1992-07-09
HU9301801D0 (en) 1993-10-28
SK62593A3 (en) 1993-10-06
FI932831L (fi) 1993-06-18
HUT68751A (en) 1995-07-28
NZ241011A (en) 1993-04-28
FI932831A7 (fi) 1993-06-18
IL100408A0 (en) 1992-09-06
KR930703450A (ko) 1993-11-30
PT99848A (pt) 1993-06-30
CZ119293A3 (en) 1994-02-16
EP0563175A1 (en) 1993-10-06
AU660161B2 (en) 1995-06-15
CA2098731A1 (en) 1992-06-19
IE914433A1 (en) 1992-07-01
JPH06503957A (ja) 1994-05-12
DK300090D0 (da) 1990-12-19
FI932831A0 (fi) 1993-06-18
MX9102684A (es) 1992-06-01
AU9134891A (en) 1992-07-22

Similar Documents

Publication Publication Date Title
HU9301801D0 (en) Method for producing synthetic leader sequences
GB9024684D0 (en) A method of communicating data
PL296827A1 (en) Method of preparing a contrast medium
EP0445692A3 (en) A method of producing monoglyceride
GB9206681D0 (en) A method of making a synthetic seismogram
EP0448033A3 (en) Method of manufacturing a magnetic head
GB2277766B (en) Method of constructing a roadway
EP0543257A3 (en) Method of manufacturing a power-misfet
OA09821A (en) Method of cementing a well
GB9108272D0 (en) Method of constructing a roadway
EP0480313A3 (en) Method of fabrication a t-gate-electrode
GB2394222B (en) A method of making a polynitramine
PL290622A1 (en) Method of obtaining a dipeptyde compound
GB9024213D0 (en) Method of producing a colouring system
GB2262268B (en) A method of clothing manufacture
GB2241621B (en) A new method of amplification
PL290392A1 (en) Method of moulding a lintel
GB9018208D0 (en) A method of tube forming
PL286457A2 (en) Method of obtaining a collagen compound
PL287634A2 (en) Method of obtaining a chiral diazacoronand
PL290421A1 (en) Method of obtaining a biopreparation polagrocyna
ZA914891B (en) Method of constructing a foundation
PL284805A2 (en) Method of obtaining a cobaltized azo dye 1:2
GB9002818D0 (en) Method of producing a dyestuff
PL287211A1 (en) Method of obtaining a flexible artificial veener