ZA954781B - Nucleic acid sequences and expression systems for heparinase II and heparinase III derived from Flavobacterium heparinum - Google Patents

Nucleic acid sequences and expression systems for heparinase II and heparinase III derived from Flavobacterium heparinum

Info

Publication number
ZA954781B
ZA954781B ZA954781A ZA954781A ZA954781B ZA 954781 B ZA954781 B ZA 954781B ZA 954781 A ZA954781 A ZA 954781A ZA 954781 A ZA954781 A ZA 954781A ZA 954781 B ZA954781 B ZA 954781B
Authority
ZA
South Africa
Prior art keywords
heparinase
nucleic acid
acid sequences
expression systems
flavobacterium heparinum
Prior art date
Application number
ZA954781A
Other languages
English (en)
Inventor
Honsheng Su
Francoise Blain
Clark Bennett
Kangfu Gu
Joseph Zimmermann
Roy Musil
Original Assignee
Ibex Technologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ibex Technologies filed Critical Ibex Technologies
Publication of ZA954781B publication Critical patent/ZA954781B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02007—Heparin lyase (4.2.2.7), i.e. heparinase I

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
ZA954781A 1994-06-10 1995-06-09 Nucleic acid sequences and expression systems for heparinase II and heparinase III derived from Flavobacterium heparinum ZA954781B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/258,639 US5681733A (en) 1994-06-10 1994-06-10 Nucleic acid sequences and expression systems for heparinase II and heparinase III derived from Flavobacterium heparinum

Publications (1)

Publication Number Publication Date
ZA954781B true ZA954781B (en) 1996-02-08

Family

ID=22981474

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA954781A ZA954781B (en) 1994-06-10 1995-06-09 Nucleic acid sequences and expression systems for heparinase II and heparinase III derived from Flavobacterium heparinum

Country Status (13)

Country Link
US (2) US5681733A (de)
EP (2) EP0763101B1 (de)
JP (2) JP3594608B2 (de)
AT (2) ATE256731T1 (de)
AU (1) AU694241B2 (de)
CA (1) CA2192159C (de)
DE (2) DE69532326T2 (de)
DK (1) DK0763101T3 (de)
ES (2) ES2279929T3 (de)
IL (1) IL114091A (de)
PT (1) PT763101E (de)
WO (1) WO1995034635A1 (de)
ZA (1) ZA954781B (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2235223A1 (en) 1995-10-30 1997-05-09 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from heparinase i
US7056504B1 (en) * 1998-08-27 2006-06-06 Massachusetts Institute Of Technology Rationally designed heparinases derived from heparinase I and II
EP1109919A2 (de) * 1998-08-27 2001-06-27 Massachusetts Institute Of Technology Von heparinase i und ii abgeleitete heparinasen
JP4824170B2 (ja) * 1999-04-23 2011-11-30 マサチューセッツ インスティテュート オブ テクノロジー ポリマーを表記するためのシステムおよび方法
JP2003504428A (ja) * 1999-06-30 2003-02-04 ハミルトン シビック ホスピタルズ リサーチ ディベロップメント インコーポレイテッド クロット関連凝固因子を阻害するヘパリン組成物
WO2001053474A2 (en) * 2000-01-21 2001-07-26 Ibex Pharmaceuticals, Inc. Flavobacterium heparinum expression system
WO2001066772A2 (en) * 2000-03-08 2001-09-13 Massachusetts Institute Of Technology Heparinase iii and uses thereof
AU2001291549A1 (en) * 2000-09-08 2002-03-22 Hamilton Civic Hospitals Research Development Inc. Antithrombotic compositions
DE60138115D1 (de) 2000-09-12 2009-05-07 Massachusetts Inst Technology Verfahren und produkte, die mit niedermolekularem heparin assoziiert sind
US7709461B2 (en) 2000-10-18 2010-05-04 Massachusetts Institute Of Technology Methods and products related to pulmonary delivery of polysaccharides
CA2493509C (en) * 2002-06-03 2010-03-09 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from chondroitinase b
KR100804584B1 (ko) 2005-03-17 2008-02-20 재단법인서울대학교산학협력재단 글리코자미노글리칸 분해효소들의 정제방법
CN100355893C (zh) * 2005-08-18 2007-12-19 清华大学 一种表达肝素酶的方法及其专用表达载体
NZ567190A (en) * 2005-10-06 2012-07-27 Dompe Pha R Ma Spa Res & Mfg Method of producing a recombinant protein comprising transforming an E. Coli host cell lacking a gene encoding pyrC with a vector comprising a gene encoding the recombinant protein and a gene encoding E. coli pyrC
US7767420B2 (en) 2005-11-03 2010-08-03 Momenta Pharmaceuticals, Inc. Heparan sulfate glycosaminoglycan lyase and uses thereof
EP2447285A3 (de) 2006-05-25 2013-01-16 Momenta Pharmaceuticals, Inc. Heparinzusammensetzung mit geringem Molekulargewicht und Anwendungen dafür
CN101723874B (zh) * 2008-10-31 2013-09-11 深圳迈瑞生物医疗电子股份有限公司 花菁类化合物及其在生物样品染色中的用途
CN101750476B (zh) * 2008-12-08 2015-06-03 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法
CN102115456B (zh) * 2009-12-30 2014-08-20 深圳迈瑞生物医疗电子股份有限公司 花菁类化合物，包含所述化合物的组合物及其在细胞检测中的用途
CN102888391B (zh) * 2012-10-30 2015-01-21 深圳市海普瑞药业股份有限公司 肝素酶ⅱ的固定化方法
JP6863626B2 (ja) * 2016-12-22 2021-04-21 シェンツェン ヘパリンク ファーマスーティカル グループ カンパニー リミテッドＳｈｅｎｚｈｅｎ Ｈｅｐａｌｉｎｋ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｇｒｏｕｐ Ｃｏ．， Ｌｔｄ． ヘパリナーゼ産生シュードモナス・スタッツェリ株及びそれから得られるヘパリナーゼ
WO2021128254A1 (zh) * 2019-12-27 2021-07-01 深圳市天道医药有限公司 一种肝素酶iii的制备方法
CN114703168B (zh) * 2022-03-02 2023-08-18 刘颖 一种肝素酶iii

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169772A (en) * 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
US5262325A (en) * 1991-04-04 1993-11-16 Ibex Technologies, Inc. Method for the enzymatic neutralization of heparin
US5714376A (en) * 1991-10-23 1998-02-03 Massachusetts Institute Of Technology Heparinase gene from flavobacterium heparinum
US5389539A (en) * 1992-11-30 1995-02-14 Massachusetts Institute Of Technology Purification of heparinase I, II, and III from Flavobacterium heparinum

Also Published As

Publication number Publication date
JP3588359B2 (ja) 2004-11-10
DE69535400D1 (de) 2007-04-05
JP2004222727A (ja) 2004-08-12
ES2279929T3 (es) 2007-09-01
DE69532326T2 (de) 2005-02-10
EP0763101A1 (de) 1997-03-19
US5919693A (en) 1999-07-06
IL114091A0 (en) 1995-10-31
US5681733A (en) 1997-10-28
EP0763101B1 (de) 2003-12-17
PT763101E (pt) 2004-05-31
AU694241B2 (en) 1998-07-16
AU2771095A (en) 1996-01-05
JP3594608B2 (ja) 2004-12-02
ES2213159T3 (es) 2004-08-16
EP1405914A1 (de) 2004-04-07
ATE256731T1 (de) 2004-01-15
ATE354661T1 (de) 2007-03-15
CA2192159A1 (en) 1995-12-21
CA2192159C (en) 2010-04-27
WO1995034635A1 (en) 1995-12-21
DE69535400T2 (de) 2007-10-31
EP1405914B1 (de) 2007-02-21
DK0763101T3 (da) 2004-04-13
DE69532326D1 (de) 2004-01-29
JPH10511841A (ja) 1998-11-17
EP0763101A4 (de) 1999-09-08
IL114091A (en) 2005-07-25

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