ATE354661T1 - Nukleinsäuresequenzen und expressionssysteme für heparinase ii aus flavobacterium heparinum - Google Patents

Nukleinsäuresequenzen und expressionssysteme für heparinase ii aus flavobacterium heparinum

Info

Publication number
ATE354661T1
ATE354661T1 AT03078100T AT03078100T ATE354661T1 AT E354661 T1 ATE354661 T1 AT E354661T1 AT 03078100 T AT03078100 T AT 03078100T AT 03078100 T AT03078100 T AT 03078100T AT E354661 T1 ATE354661 T1 AT E354661T1
Authority
AT
Austria
Prior art keywords
heparinase
nucleic acid
acid sequences
expression systems
flavobacterium heparinum
Prior art date
Application number
AT03078100T
Other languages
English (en)
Inventor
Hongsheng Su
Francoise Blain
Clark Bennett
Kangfu Gu
Roy Musil
Original Assignee
Biomarin Pharm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomarin Pharm Inc filed Critical Biomarin Pharm Inc
Application granted granted Critical
Publication of ATE354661T1 publication Critical patent/ATE354661T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02007—Heparin lyase (4.2.2.7), i.e. heparinase I
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT03078100T 1994-06-10 1995-06-09 Nukleinsäuresequenzen und expressionssysteme für heparinase ii aus flavobacterium heparinum ATE354661T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/258,639 US5681733A (en) 1994-06-10 1994-06-10 Nucleic acid sequences and expression systems for heparinase II and heparinase III derived from Flavobacterium heparinum

Publications (1)

Publication Number Publication Date
ATE354661T1 true ATE354661T1 (de) 2007-03-15

Family

ID=22981474

Family Applications (2)

Application Number Title Priority Date Filing Date
AT95923026T ATE256731T1 (de) 1994-06-10 1995-06-09 Nukleinsäuresequenzen und expressionssysteme für heparinase iii aus flavobacterium heparinum
AT03078100T ATE354661T1 (de) 1994-06-10 1995-06-09 Nukleinsäuresequenzen und expressionssysteme für heparinase ii aus flavobacterium heparinum

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT95923026T ATE256731T1 (de) 1994-06-10 1995-06-09 Nukleinsäuresequenzen und expressionssysteme für heparinase iii aus flavobacterium heparinum

Country Status (13)

Country Link
US (2) US5681733A (de)
EP (2) EP1405914B1 (de)
JP (2) JP3594608B2 (de)
AT (2) ATE256731T1 (de)
AU (1) AU694241B2 (de)
CA (1) CA2192159C (de)
DE (2) DE69532326T2 (de)
DK (1) DK0763101T3 (de)
ES (2) ES2213159T3 (de)
IL (1) IL114091A (de)
PT (1) PT763101E (de)
WO (1) WO1995034635A1 (de)
ZA (1) ZA954781B (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6217863B1 (en) 1995-10-30 2001-04-17 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from heparinase I
US7056504B1 (en) * 1998-08-27 2006-06-06 Massachusetts Institute Of Technology Rationally designed heparinases derived from heparinase I and II
JP2003527822A (ja) * 1998-08-27 2003-09-24 マサチューセッツ インスティテュート オブ テクノロジー ヘパリナーゼｉおよびｉｉ由来の合理的に設計されたヘパリナーゼ
CA2370539C (en) * 1999-04-23 2009-01-06 Massachusetts Institute Of Technology System and method for notating polymers
CA2377734A1 (en) * 1999-06-30 2001-01-11 Hamilton Civic Hospitals Research Development, Inc. Heparin compositions that inhibit clot associated coagulation factors
DE60127381D1 (de) * 2000-01-21 2007-05-03 Biomarin Pharm Inc Flavobakterium heparinum expressions-system
CA2402160C (en) * 2000-03-08 2012-02-14 Massachusetts Institute Of Technology Heparinase iii and uses thereof
CA2420890A1 (en) * 2000-09-08 2002-03-14 Hamilton Civic Hospitals Research Development, Inc. Antithrombotic compositions
DE60138115D1 (de) * 2000-09-12 2009-05-07 Massachusetts Inst Technology Verfahren und produkte, die mit niedermolekularem heparin assoziiert sind
AU2002224408B2 (en) 2000-10-18 2007-08-23 Massachusetts Institute Of Technology Methods and products related to pulmonary delivery of polysaccharides
DE60334220D1 (de) * 2002-06-03 2010-10-28 Massachusetts Inst Technology Rationell konstruierte, von chondroitinase b abgeleitete lyasen
KR100804584B1 (ko) 2005-03-17 2008-02-20 재단법인서울대학교산학협력재단 글리코자미노글리칸 분해효소들의 정제방법
CN100355893C (zh) * 2005-08-18 2007-12-19 清华大学 一种表达肝素酶的方法及其专用表达载体
JP5415763B2 (ja) * 2005-10-06 2014-02-12 ドムペ・ファ．ル．マ・ソチエタ・ペル・アツィオーニ 新規選択系
US7767420B2 (en) 2005-11-03 2010-08-03 Momenta Pharmaceuticals, Inc. Heparan sulfate glycosaminoglycan lyase and uses thereof
CN101495517B (zh) 2006-05-25 2012-10-10 莫曼塔医药品有限公司 低分子量肝素及其用途
CN101723874B (zh) * 2008-10-31 2013-09-11 深圳迈瑞生物医疗电子股份有限公司 花菁类化合物及其在生物样品染色中的用途
CN101750476B (zh) * 2008-12-08 2015-06-03 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法
CN102115456B (zh) * 2009-12-30 2014-08-20 深圳迈瑞生物医疗电子股份有限公司 花菁类化合物，包含所述化合物的组合物及其在细胞检测中的用途
CN102888391B (zh) * 2012-10-30 2015-01-21 深圳市海普瑞药业股份有限公司 肝素酶ⅱ的固定化方法
EP3561048A4 (de) * 2016-12-22 2020-08-12 Shenzhen Hepalink Pharmaceutical Group Co., Ltd. Heparinase-produzierender pseudomonas-stutzeri-stamm und heparinase daraus
WO2021128254A1 (zh) * 2019-12-27 2021-07-01 深圳市天道医药有限公司 一种肝素酶iii的制备方法
CN114703168B (zh) * 2022-03-02 2023-08-18 刘颖 一种肝素酶iii

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169772A (en) * 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
US5262325A (en) * 1991-04-04 1993-11-16 Ibex Technologies, Inc. Method for the enzymatic neutralization of heparin
US5714376A (en) * 1991-10-23 1998-02-03 Massachusetts Institute Of Technology Heparinase gene from flavobacterium heparinum
US5389539A (en) * 1992-11-30 1995-02-14 Massachusetts Institute Of Technology Purification of heparinase I, II, and III from Flavobacterium heparinum

Also Published As

Publication number Publication date
DK0763101T3 (da) 2004-04-13
US5919693A (en) 1999-07-06
EP1405914B1 (de) 2007-02-21
CA2192159C (en) 2010-04-27
JPH10511841A (ja) 1998-11-17
WO1995034635A1 (en) 1995-12-21
JP3588359B2 (ja) 2004-11-10
IL114091A0 (en) 1995-10-31
ATE256731T1 (de) 2004-01-15
DE69532326T2 (de) 2005-02-10
DE69532326D1 (de) 2004-01-29
US5681733A (en) 1997-10-28
ZA954781B (en) 1996-02-08
ES2279929T3 (es) 2007-09-01
EP0763101B1 (de) 2003-12-17
DE69535400D1 (de) 2007-04-05
ES2213159T3 (es) 2004-08-16
IL114091A (en) 2005-07-25
DE69535400T2 (de) 2007-10-31
JP3594608B2 (ja) 2004-12-02
PT763101E (pt) 2004-05-31
AU694241B2 (en) 1998-07-16
CA2192159A1 (en) 1995-12-21
EP0763101A1 (de) 1997-03-19
EP0763101A4 (de) 1999-09-08
EP1405914A1 (de) 2004-04-07
AU2771095A (en) 1996-01-05
JP2004222727A (ja) 2004-08-12

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