ATE103637T1 - Verfahren zur herstellung von fokirestriktionsendonuklease und -methylase. - Google Patents

Verfahren zur herstellung von fokirestriktionsendonuklease und -methylase.

Info

Publication number
ATE103637T1
ATE103637T1 AT88311917T AT88311917T ATE103637T1 AT E103637 T1 ATE103637 T1 AT E103637T1 AT 88311917 T AT88311917 T AT 88311917T AT 88311917 T AT88311917 T AT 88311917T AT E103637 T1 ATE103637 T1 AT E103637T1
Authority
AT
Austria
Prior art keywords
restriction endonuclease
methylase
production
host
foki
Prior art date
Application number
AT88311917T
Other languages
English (en)
Inventor
Mary Looney
Geoffrey Wilson
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of ATE103637T1 publication Critical patent/ATE103637T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT88311917T 1987-12-17 1988-12-16 Verfahren zur herstellung von fokirestriktionsendonuklease und -methylase. ATE103637T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/134,236 US4999294A (en) 1987-12-17 1987-12-17 Method for producing the FokI restriction endonuclease and methylase
EP88311917A EP0321266B1 (de) 1987-12-17 1988-12-16 Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase

Publications (1)

Publication Number Publication Date
ATE103637T1 true ATE103637T1 (de) 1994-04-15

Family

ID=22462394

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88311917T ATE103637T1 (de) 1987-12-17 1988-12-16 Verfahren zur herstellung von fokirestriktionsendonuklease und -methylase.

Country Status (5)

Country Link
US (1) US4999294A (de)
EP (1) EP0321266B1 (de)
JP (1) JPH022370A (de)
AT (1) ATE103637T1 (de)
DE (1) DE3888804T2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5238821A (en) * 1989-05-16 1993-08-24 Genzyme Corporation Endo F-free PNGase
US5298404A (en) * 1989-10-13 1994-03-29 New England Biolabs, Inc. Method for producing the Hpa I restriction endonuclease and methylase
US5192676A (en) * 1991-02-05 1993-03-09 New England Biolabs, Inc. Type ii restriction endonuclease, asci, obtainable from arthrobacter species and a process for producing the same
US5200337A (en) * 1991-10-25 1993-04-06 New England Biolabs, Inc. Type ii restriction endonuclease, apo i, obtainable from arthrobacter protophormiae and a process for producing the same
EP0756286B1 (de) 1995-07-24 2000-01-26 STMicroelectronics S.r.l. Flash-EEPROM mit onchip-Löschung-Source-Spannungsgenerator
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
EP2548953A1 (de) 2005-08-04 2013-01-23 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2012078312A2 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
EP2748318B1 (de) 2011-08-26 2015-11-04 Gen9, Inc. Zusammensetzungen und verfahren für hochqualitative anordnung von nukleinsäuren
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
CN104685116A (zh) 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN166864B (de) * 1985-03-01 1990-07-28 New England Biolabs Inc
ATE113653T1 (de) * 1986-06-06 1994-11-15 New England Biolabs Inc Verfahren zur klonierung eines restriktionsmodifizierungssystems.

Also Published As

Publication number Publication date
EP0321266A3 (en) 1990-03-21
DE3888804T2 (de) 1994-11-10
EP0321266A2 (de) 1989-06-21
EP0321266B1 (de) 1994-03-30
JPH022370A (ja) 1990-01-08
DE3888804D1 (de) 1994-05-05
US4999294A (en) 1991-03-12

Similar Documents

Publication Publication Date Title
ATE103637T1 (de) Verfahren zur herstellung von fokirestriktionsendonuklease und -methylase.
DE3751923D1 (de) Verfahren zur Klonierung von Restriktions-Modifikationssystemen
ATE238429T1 (de) Enzymatisches verfahren zur herstellung von ascorbinsäure,2-keto-l-gulonsäure und 2-keto-l- gulonsäure-estern
DE3853145D1 (de) Verfahren zur Herstellung von xbaI-Restriktionsendonuklease und -methylase.
ATE196776T1 (de) Neue mikroorganismen, deren verwendung und verfahren zur herstellung von l-alpha-aminosäuren
DE69110952D1 (de) Penicillin-G-Acylase, dafür kodierendes Gen und Verfahren zur Herstellung von diesem Enzym.
ATE106448T1 (de) Verfahren zur herstellung von hinpi- restriktionsendonuklease und -methylase.
ATE103329T1 (de) Verfahren zur herstellung von hgiairestriktionsendonuklease und -methylase.
ATE8142T1 (de) Verfahren zur herstellung von isosorbid-5-nitrat.
DE3581218D1 (de) Verfahren zur herstellung von 6-hydroxynikotinsaeure.
EP0321270A3 (de) Verfahren zur Herstellung von HhaI-Restriktionsendonuklease und -methylase
ATE101647T1 (de) Verfahren zur herstellung von aseirestriktionsendonuklease und -methylase.
HUT59727A (en) Improved enzymatic oxidizing process for producing glutaryl-7-amino-cephalosporanic acid from cephalosporin c
DE3853143D1 (de) Verfahren zur Herstellung von AccI-Restriktionsendonuklease und -methylase.
EP0324248A3 (de) Verfahren zur Herstellung von FnuDI-Restriktionsendonuklease und -methylase
DE3688302D1 (de) Verfahren zur herstellung von 7-aca-derivaten.
ATE171214T1 (de) Verfahren zur herstellung einer alpha- acetomilchsäure
ATE76662T1 (de) Enzymatisches verfahren zur herstellung von 3substituierten cephalosporinen.
ATE233751T1 (de) Verfahren zur herstellung und zwischenprodukt der 2-(10,11-dihydro-10-oxodibenzo(b,f)thiepin-2-yl - propionsäure
EP0355981A3 (de) Verfahren zur Herstellung von Nla IV Restriktionsendonuklease und -methylase
EP0712933A3 (de) Für die FseI Restriktionsendonuklease kodierende, isolierte DNA und Verfahren zu ihren Herstellung
ATE196927T1 (de) Verfahren zur herstellung von proteaseinhibitoren
EP0321268A3 (de) Verfahren zur Herstellung von BanI-Restriktionsendonuklease und -methylase
ATE219142T1 (de) Verfahren zur herstellung von trans-4-hydroxy-l- prolin
DE3854373D1 (de) Verfahren zur Herstellung der Afl II Restriktionsendonuklease.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties