ATE109204T1 - Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung. - Google Patents

Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.

Info

Publication number
ATE109204T1
ATE109204T1 AT90904029T AT90904029T ATE109204T1 AT E109204 T1 ATE109204 T1 AT E109204T1 AT 90904029 T AT90904029 T AT 90904029T AT 90904029 T AT90904029 T AT 90904029T AT E109204 T1 ATE109204 T1 AT E109204T1
Authority
AT
Austria
Prior art keywords
genomic dna
cohesive
restriction
linker
methods
Prior art date
Application number
AT90904029T
Other languages
English (en)
Inventor
Harvey I Miller
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE109204T1 publication Critical patent/ATE109204T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Control And Other Processes For Unpacking Of Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
AT90904029T 1989-03-03 1990-02-15 Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung. ATE109204T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US31838589A 1989-03-03 1989-03-03

Publications (1)

Publication Number Publication Date
ATE109204T1 true ATE109204T1 (de) 1994-08-15

Family

ID=23237960

Family Applications (2)

Application Number Title Priority Date Filing Date
AT93119315T ATE298795T1 (de) 1989-03-03 1990-02-15 Genomische klonierung und kartographie
AT90904029T ATE109204T1 (de) 1989-03-03 1990-02-15 Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT93119315T ATE298795T1 (de) 1989-03-03 1990-02-15 Genomische klonierung und kartographie

Country Status (7)

Country Link
EP (2) EP0593095B1 (de)
JP (1) JP3030417B2 (de)
AT (2) ATE298795T1 (de)
DE (2) DE69034197T2 (de)
DK (2) DK0461155T3 (de)
ES (1) ES2058893T3 (de)
WO (1) WO1990010064A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
DE3929030A1 (de) * 1989-09-01 1991-03-07 Boehringer Mannheim Gmbh Verfahren zur vermehrung von nukleinsaeuren
DE4106473C2 (de) * 1991-03-01 1994-03-10 Boehringer Mannheim Gmbh Verfahren zur Herstellung in vitro replizierbarer Nukleinsäuren
US20100267023A1 (en) 1992-09-24 2010-10-21 Keygene N.V. Selective restriction fragment amplification: fingerprinting
CA2119557C (en) * 1991-09-24 2004-02-24 Marc Zabeau Selective restriction fragment amplification: a general method for dna fingerprinting
IT1262937B (it) * 1992-04-13 1996-07-22 Enichem Spa Selezione genica in vitro mediante vettori ad rna
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
AT402203B (de) * 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
US5550025A (en) * 1995-07-19 1996-08-27 Becton, Dickinson And Company Detection of hydrophobic amplification products by extraction into an organic phase
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US6136537A (en) * 1998-02-23 2000-10-24 Macevicz; Stephen C. Gene expression analysis
US8367322B2 (en) 1999-01-06 2013-02-05 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
AU780119B2 (en) * 1999-01-06 2005-03-03 Cornell Research Foundation Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
GB0114852D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happiar mapping
US7229800B2 (en) 2004-04-16 2007-06-12 Becton, Dickinson And Company Neisseria gonorrhoeae assay
US8637655B2 (en) 2009-08-13 2014-01-28 Life Technologies Corporation Amelogenin SNP on chromosome X
US9029088B2 (en) 2009-10-15 2015-05-12 Life Technologies Corporation Human single nucleotide polymorphisms within CODIS loci D13S317, TH01, vWA, D12S391, and D6S1043
US20110165568A1 (en) 2009-12-31 2011-07-07 Life Technologies Corporation Sequences of e.coli 055:h7 genome
WO2012040403A1 (en) 2010-09-21 2012-03-29 Life Technologies Corporation Se33 mutations impacting genotype concordance
EP2635696B1 (de) 2010-11-01 2017-11-29 Becton Dickinson and Company Test auf gardnerella vaginalis
ES2387295B1 (es) * 2011-03-03 2013-07-31 Consejo Superior De Investigaciones Científicas (Csic) MÉTODO DE AMPLIFICACIÓN DE ADN BASADO EN LOS ORÍGENES DE REPLICACIÓN DEL BACTERIÓFAGO Phi29 Y SECUENCIAS NUCLEOTÍDICAS ASOCIADAS.
US20120322676A1 (en) 2011-06-17 2012-12-20 Life Technologies Corporation Compositions and methods for detection of cronobacter spp. and cronobacter species and strains
DK2748332T3 (en) 2011-08-24 2018-07-16 Oxoid Ltd COMPOSITIONS AND METHODS FOR DETECTING MULTIPLE MICROORGANISMS
EP3392347A1 (de) 2012-04-02 2018-10-24 Life Technologies Corporation Zusammensetzungen und verfahren zur detektion von vogelmykobakterium-paratuberkulose
US20140005061A1 (en) 2012-06-29 2014-01-02 Life Technologies Corporation Compositions and methods for detection of multiple microorganisms
EP2867377B1 (de) 2012-07-02 2019-08-21 The Translational Genomics Research Institute Primer, assays und verfahren zum nachweis eines e.-coli-subtyps
US10844441B2 (en) 2016-05-16 2020-11-24 Life Technologies Corporation Penta e polymorphisms for human identification

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve

Also Published As

Publication number Publication date
EP0593095B1 (de) 2005-06-29
JPH04503752A (ja) 1992-07-09
DE69011101T2 (de) 1995-01-26
EP0593095A1 (de) 1994-04-20
DK0593095T3 (da) 2005-10-17
JP3030417B2 (ja) 2000-04-10
EP0461155A1 (de) 1991-12-18
DE69034197D1 (de) 2005-08-04
DK0461155T3 (da) 1994-11-21
WO1990010064A1 (en) 1990-09-07
DE69011101D1 (de) 1994-09-01
ATE298795T1 (de) 2005-07-15
ES2058893T3 (es) 1994-11-01
DE69034197T2 (de) 2006-04-27
EP0461155B1 (de) 1994-07-27

Similar Documents

Publication Publication Date Title
ATE109204T1 (de) Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.
Fried et al. Polyoma virus-a study of wild-type, mutant and defective DNAs
Jensch et al. Endonuclease VII resolves Y‐junctions in branched DNA in vitro.
DE50114507D1 (de) Nukleinsäure-Linker und deren Verwendung in der Gensynthese
Been et al. Breakage of single-stranded DNA by eukaryotic type 1 topoisomerase occurs only at regions with the potential for base-pairing
KR920701478A (ko) 핵산서열의 증폭방법
ATE106949T1 (de) Verfahren zum klonieren und reagenziensatz.
CN105624328B (zh) 鉴定番茄叶霉病抗性的高通量分子标记及其标记方法与应用
DE69433487D1 (de) Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
US4843003A (en) Process for producing shortened target DNA fragments usable in sequencing large DNA segments
DE59609841D1 (de) Verfahren zur Gattungs- und speziesspezifische Identifizierung von Legionellen
CN105671190A (zh) 鉴定番茄颈腐根腐病抗性的高通量分子标记及其标记方法与应用
Sutcliffe et al. The cleavage site of the restriction endonuclease Ava II
Leung et al. A restriction enzyme from Fusobacterium nucleatum 4H which recognizes GCNGC
Takanami et al. Studies on bacteriophage fd DNA: I. A cleavage map of the fd genome
CN119710061A (zh) 基于RPA-CRISPR/Cas12a的可视化检测柑橘褐斑病菌的检测试剂盒及检测方法
CN118006584A (zh) CRISPR基因座完全缺失Cas1、Cas2和Cas4的可编程核酸酶及其应用
CN117467661A (zh) 一种pcr扩增引物、试剂盒及其应用
CN100413978C (zh) 基于dna切割过程的双链dna序列测定的方法
STANLEY et al. The Binding Site of Ribosomal Protein L1 from Escherichia coli on the 23‐S Ribosomal RNA from Bacillus stearothermophilus: A Possible Base‐Pairing Scheme Differing from That Proposed for Escherichia coli
Kiko et al. Physical mapping of the restriction fragments obtained from bacteriophage T4 dC-DNA with the restriction endonucleases Sma I, Kpn I and Bgl II
CN116162731A (zh) 基于锁式探针的落叶松枯梢病菌滚环扩增引物组及应用
US4889799A (en) Reagent kit producing shortened target DNA segments usable in sequencing large DNA segments
KR102900467B1 (ko) 토홀드 매개 가닥 치환 기반의 rpa-crispr 핵산 검출용 조성물 및 검출 방법
CN116254327B (zh) 一种Argonaute介导链置换指数扩增方法及应用

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time