ATE109204T1 - Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung. - Google Patents
Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.Info
- Publication number
- ATE109204T1 ATE109204T1 AT90904029T AT90904029T ATE109204T1 AT E109204 T1 ATE109204 T1 AT E109204T1 AT 90904029 T AT90904029 T AT 90904029T AT 90904029 T AT90904029 T AT 90904029T AT E109204 T1 ATE109204 T1 AT E109204T1
- Authority
- AT
- Austria
- Prior art keywords
- genomic dna
- cohesive
- restriction
- linker
- methods
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000010367 cloning Methods 0.000 title abstract 2
- 238000013507 mapping Methods 0.000 title abstract 2
- 230000004544 DNA amplification Effects 0.000 title 1
- 238000000338 in vitro Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 239000012634 fragment Substances 0.000 abstract 5
- 108091008146 restriction endonucleases Proteins 0.000 abstract 3
- 230000007023 DNA restriction-modification system Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Control And Other Processes For Unpacking Of Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31838589A | 1989-03-03 | 1989-03-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE109204T1 true ATE109204T1 (de) | 1994-08-15 |
Family
ID=23237960
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT90904029T ATE109204T1 (de) | 1989-03-03 | 1990-02-15 | Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung. |
| AT93119315T ATE298795T1 (de) | 1989-03-03 | 1990-02-15 | Genomische klonierung und kartographie |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT93119315T ATE298795T1 (de) | 1989-03-03 | 1990-02-15 | Genomische klonierung und kartographie |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP0461155B1 (de) |
| JP (1) | JP3030417B2 (de) |
| AT (2) | ATE109204T1 (de) |
| DE (2) | DE69034197T2 (de) |
| DK (2) | DK0461155T3 (de) |
| ES (1) | ES2058893T3 (de) |
| WO (1) | WO1990010064A1 (de) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5198543A (en) * | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| US5001050A (en) * | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
| DE3929030A1 (de) * | 1989-09-01 | 1991-03-07 | Boehringer Mannheim Gmbh | Verfahren zur vermehrung von nukleinsaeuren |
| DE4106473C2 (de) * | 1991-03-01 | 1994-03-10 | Boehringer Mannheim Gmbh | Verfahren zur Herstellung in vitro replizierbarer Nukleinsäuren |
| ZA927323B (en) * | 1991-09-24 | 1993-08-30 | Keygene Nv | Selective restriction fragment amplification: A general method for DNA fingerprinting |
| US20100267023A1 (en) | 1992-09-24 | 2010-10-21 | Keygene N.V. | Selective restriction fragment amplification: fingerprinting |
| IT1262937B (it) * | 1992-04-13 | 1996-07-22 | Enichem Spa | Selezione genica in vitro mediante vettori ad rna |
| US5457027A (en) * | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
| US5710000A (en) | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| AT402203B (de) * | 1995-06-13 | 1997-03-25 | Himmler Gottfried Dipl Ing Dr | Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren |
| US5550025A (en) * | 1995-07-19 | 1996-08-27 | Becton, Dickinson And Company | Detection of hydrophobic amplification products by extraction into an organic phase |
| US6291164B1 (en) | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
| US6054276A (en) | 1998-02-23 | 2000-04-25 | Macevicz; Stephen C. | DNA restriction site mapping |
| US6136537A (en) * | 1998-02-23 | 2000-10-24 | Macevicz; Stephen C. | Gene expression analysis |
| CA2356697C (en) | 1999-01-06 | 2010-06-22 | Cornell Research Foundation, Inc. | Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing |
| US8367322B2 (en) | 1999-01-06 | 2013-02-05 | Cornell Research Foundation, Inc. | Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing |
| GB0114852D0 (en) * | 2001-06-18 | 2001-08-08 | Medical Res Council | Happiar mapping |
| US7229800B2 (en) | 2004-04-16 | 2007-06-12 | Becton, Dickinson And Company | Neisseria gonorrhoeae assay |
| EP2789693B1 (de) | 2009-08-13 | 2017-10-04 | Life Technologies Corporation | Amelogenin-SNP auf X-Chromosomen |
| EP2488668B1 (de) | 2009-10-15 | 2023-07-26 | Life Technologies Corporation | Neue menschliche einzelnukleotid-polymorphismen |
| WO2011082325A2 (en) | 2009-12-31 | 2011-07-07 | Life Technologies Corporation | Sequences of e.coli 055:h7 genome |
| EP2937423A1 (de) | 2010-09-21 | 2015-10-28 | Life Technologies Corporation | Se33-mutationen mit auswirkung auf die genotypkonkordanz |
| CA2816311C (en) | 2010-11-01 | 2018-07-17 | Becton, Dickinson And Company | Gardnerella vaginalis assay |
| ES2387295B1 (es) | 2011-03-03 | 2013-07-31 | Consejo Superior De Investigaciones Científicas (Csic) | MÉTODO DE AMPLIFICACIÓN DE ADN BASADO EN LOS ORÍGENES DE REPLICACIÓN DEL BACTERIÓFAGO Phi29 Y SECUENCIAS NUCLEOTÍDICAS ASOCIADAS. |
| WO2012174119A2 (en) | 2011-06-17 | 2012-12-20 | Life Technologies Corporation | Compositions and methods for detection of cronobacter spp. and cronobacter species and strains |
| ES2680143T3 (es) | 2011-08-24 | 2018-09-04 | Oxoid Limited | Composiciones y métodos para la detección de múltiples microorganismos |
| EP3392347A1 (de) | 2012-04-02 | 2018-10-24 | Life Technologies Corporation | Zusammensetzungen und verfahren zur detektion von vogelmykobakterium-paratuberkulose |
| US20140005061A1 (en) | 2012-06-29 | 2014-01-02 | Life Technologies Corporation | Compositions and methods for detection of multiple microorganisms |
| US9834823B2 (en) | 2012-07-02 | 2017-12-05 | The Translational Genomics Research Institute | Primers, assays and methods for detecting an E. coli subtype |
| US10844441B2 (en) | 2016-05-16 | 2020-11-24 | Life Technologies Corporation | Penta e polymorphisms for human identification |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK171161B1 (da) * | 1985-03-28 | 1996-07-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
-
1990
- 1990-02-15 DK DK90904029.7T patent/DK0461155T3/da active
- 1990-02-15 DE DE69034197T patent/DE69034197T2/de not_active Expired - Lifetime
- 1990-02-15 EP EP90904029A patent/EP0461155B1/de not_active Expired - Lifetime
- 1990-02-15 WO PCT/US1990/000942 patent/WO1990010064A1/en not_active Ceased
- 1990-02-15 JP JP2504392A patent/JP3030417B2/ja not_active Expired - Lifetime
- 1990-02-15 EP EP93119315A patent/EP0593095B1/de not_active Expired - Lifetime
- 1990-02-15 ES ES90904029T patent/ES2058893T3/es not_active Expired - Lifetime
- 1990-02-15 DK DK93119315T patent/DK0593095T3/da active
- 1990-02-15 DE DE69011101T patent/DE69011101T2/de not_active Expired - Lifetime
- 1990-02-15 AT AT90904029T patent/ATE109204T1/de not_active IP Right Cessation
- 1990-02-15 AT AT93119315T patent/ATE298795T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ATE298795T1 (de) | 2005-07-15 |
| JPH04503752A (ja) | 1992-07-09 |
| EP0593095B1 (de) | 2005-06-29 |
| DK0593095T3 (da) | 2005-10-17 |
| ES2058893T3 (es) | 1994-11-01 |
| DE69011101D1 (de) | 1994-09-01 |
| DK0461155T3 (da) | 1994-11-21 |
| EP0461155A1 (de) | 1991-12-18 |
| EP0461155B1 (de) | 1994-07-27 |
| WO1990010064A1 (en) | 1990-09-07 |
| DE69034197T2 (de) | 2006-04-27 |
| EP0593095A1 (de) | 1994-04-20 |
| DE69011101T2 (de) | 1995-01-26 |
| JP3030417B2 (ja) | 2000-04-10 |
| DE69034197D1 (de) | 2005-08-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EELA | Cancelled due to lapse of time |