ATE113077T1 - Stabile integration von dna in bakterielle genome. - Google Patents
Stabile integration von dna in bakterielle genome.Info
- Publication number
- ATE113077T1 ATE113077T1 AT91901587T AT91901587T ATE113077T1 AT E113077 T1 ATE113077 T1 AT E113077T1 AT 91901587 T AT91901587 T AT 91901587T AT 91901587 T AT91901587 T AT 91901587T AT E113077 T1 ATE113077 T1 AT E113077T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- stable integration
- replication
- bacterial genomes
- origin
- Prior art date
Links
- 230000001580 bacterial effect Effects 0.000 title abstract 2
- 230000010354 integration Effects 0.000 title 1
- 230000010076 replication Effects 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 229940012229 genone Drugs 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000003362 replicative effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK639689A DK639689D0 (da) | 1989-12-18 | 1989-12-18 | Indfoering af dna i celler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE113077T1 true ATE113077T1 (de) | 1994-11-15 |
Family
ID=8148687
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT91901587T ATE113077T1 (de) | 1989-12-18 | 1990-12-18 | Stabile integration von dna in bakterielle genome. |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0506780B2 (de) |
| JP (1) | JP3162707B2 (de) |
| AT (1) | ATE113077T1 (de) |
| AU (1) | AU643741B2 (de) |
| CA (1) | CA2070676A1 (de) |
| DE (1) | DE69013520T3 (de) |
| DK (2) | DK639689D0 (de) |
| ES (1) | ES2064077T5 (de) |
| FI (1) | FI112502B (de) |
| NO (1) | NO922381L (de) |
| WO (1) | WO1991009129A1 (de) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2665711B1 (fr) * | 1990-08-08 | 1993-08-13 | Centre Nat Rech Scient | Integron de corynebacterie, procede de transformation d'une corynebacterie par ledit integron et corynebacterie obtenue. |
| IL110299A0 (en) | 1993-07-15 | 1994-10-21 | Novo Nordisk Entotech Inc | Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity |
| AU5001496A (en) * | 1995-03-22 | 1996-10-08 | Novo Nordisk A/S | Introduction of dna into bacillus strains by conjugation |
| FR2736066B1 (fr) * | 1995-06-30 | 1998-11-20 | Ajinomoto Kk | Procede d'amplification d'un gene par transposon artificiel, bacterie coryneforme obtenue par ce procede et procede de production d'un acide amine a l'aide de cette bacterie |
| EP0878552A1 (de) | 1997-05-13 | 1998-11-18 | Erasmus Universiteit Rotterdam | Molekularer Nachweis von chromosomalen Veränderungen |
| CN100494363C (zh) | 1997-12-22 | 2009-06-03 | 诺维信公司 | 糖氧化酶及其在焙烤中的用途 |
| EP1078098A1 (de) | 1998-05-04 | 2001-02-28 | Dako A/S | Verfahren und sonden zur detektion chromosomaler aberrationen |
| EP2284272B1 (de) | 1998-06-10 | 2014-08-13 | Novozymes A/S | Mannanasen |
| JP3958884B2 (ja) | 1998-09-11 | 2007-08-15 | 株式会社林原生物化学研究所 | 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法 |
| JP5043254B2 (ja) | 1998-10-26 | 2012-10-10 | ノボザイムス アクティーゼルスカブ | 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング |
| EP1297170B1 (de) | 2000-06-23 | 2016-03-02 | Novozymes A/S | Verfahren zur stabilen chromosomalen integration von mehreren kopien von genen |
| AU2002311012A1 (en) | 2001-06-06 | 2002-12-16 | Novozymes A/S | Endo-beta-1,4-glucanase from bacillus |
| US7521204B2 (en) | 2002-04-10 | 2009-04-21 | Novozymes A/S | Bacillus host cell |
| WO2003087149A2 (en) | 2002-04-10 | 2003-10-23 | Novozymes A/S | Bacillus licheniformis mutant host cell |
| AU2003223928A1 (en) * | 2002-05-07 | 2003-11-11 | Novozymes A/S | Homologous recombination into bacterium for the generation of polynucleotide libraries |
| CN1738899A (zh) | 2002-12-11 | 2006-02-22 | 诺和酶股份有限公司 | 洗涤剂组合物 |
| DK1694846T3 (en) | 2003-12-10 | 2014-11-17 | Novozymes As | Cell with enhanced secretion mediated by MrgA protein or a homolog |
| WO2005123915A1 (en) | 2004-06-21 | 2005-12-29 | Novozymes A/S | Stably maintained multiple copies of at least two orf in the same orientation |
| CN101595214B (zh) | 2006-11-29 | 2013-06-12 | 诺维信股份有限公司 | 改进向细菌细胞中导入dna的方法 |
| WO2008140615A2 (en) | 2006-12-21 | 2008-11-20 | Novozymes, Inc. | Modified messenger rna stabilizing sequences for expressing genes in bacterial cells |
| CN102762723A (zh) | 2009-12-21 | 2012-10-31 | 诺维信股份有限公司 | 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法 |
| EP3532619A1 (de) | 2016-10-25 | 2019-09-04 | Novozymes A/S | Flp-vermittelte genomische integration |
| WO2019016052A1 (en) | 2017-07-21 | 2019-01-24 | Basf Se | HETEROLOGOUS EXPRESSION PROMOTER |
| EP3655528A1 (de) | 2017-07-21 | 2020-05-27 | Basf Se | Verfahren zur transformation von bakterienzellen |
| EP3707252A1 (de) | 2017-11-10 | 2020-09-16 | Novozymes A/S | Temperaturempfindliches cas9-protein |
| US20210017544A1 (en) | 2017-12-22 | 2021-01-21 | Novozymes A/S | Counter-Selection by Inhibition of Conditionally Essential Genes |
| EP3918086A1 (de) | 2019-01-30 | 2021-12-08 | Novozymes A/S | Verwandte foldase-koexpression |
| EP3927837A1 (de) | 2019-02-20 | 2021-12-29 | Basf Se | Industrielles fermentationsverfahren für bacillus unter verwendung eines definierten mediums und einer magnesiumzufuhr |
| EP3927809A1 (de) | 2019-02-20 | 2021-12-29 | Basf Se | Industrielles fermentationsverfahren für bacillus unter verwendung eines definierten mediums und spurenelementezufuhr |
| US20220170003A1 (en) | 2019-03-08 | 2022-06-02 | Novozymes A/S | Means And Methods For Improving Protease Expression |
| WO2020229191A1 (en) | 2019-05-15 | 2020-11-19 | Novozymes A/S | Temperature-sensitive rna-guided endonuclease |
| EP3990629A1 (de) | 2019-06-25 | 2022-05-04 | Novozymes A/S | Gegenauswahl durch hemmung von bedingt essenziellen genen |
| US20220340865A1 (en) | 2019-07-02 | 2022-10-27 | Basf Se | Method for preparing a fermentation medium |
| CN116157530A (zh) | 2020-07-28 | 2023-05-23 | 巴斯夫欧洲公司 | 使用温度变化的芽孢杆菌工业发酵工艺 |
| BR112023001386A2 (pt) | 2020-07-28 | 2023-02-14 | Basf Se | Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus |
| BR112023001392A2 (pt) | 2020-07-28 | 2023-02-14 | Basf Se | Método para produzir uma proteína de interesse a partir de uma célula hospedeira de bacillus |
| US20240060110A1 (en) | 2020-10-07 | 2024-02-22 | Basf Se | Bacillus cell with reduced lipase and/or esterase side activities |
| MX2023015460A (es) | 2021-06-24 | 2024-01-18 | Basf Se | Celula huesped de bacillus mejorada con proteina rema/remb alterada. |
| CN117813316A (zh) | 2021-06-24 | 2024-04-02 | 巴斯夫欧洲公司 | 用于生产纯度更高的感兴趣的化合物的地衣芽孢杆菌宿主细胞 |
| CN117730090A (zh) | 2021-07-13 | 2024-03-19 | 诺维信公司 | 重组角质酶表达 |
| WO2023247514A1 (en) | 2022-06-22 | 2023-12-28 | Novozymes A/S | Recombinant mannanase expression |
| EP4565710A1 (de) | 2022-08-02 | 2025-06-11 | Basf Se | Verfahren zur herstellung von stabilisierten proteinen unter verwendung von bacillus-wirtszellen |
| WO2025132815A1 (en) | 2023-12-20 | 2025-06-26 | Novozymes A/S | Novel cas nucleases and polynucleotides encoding the same |
| WO2026132030A1 (en) | 2024-12-19 | 2026-06-25 | Novozymes A/S | Cas nucleases and polynucleotides encoding the same |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4631257A (en) * | 1978-12-26 | 1986-12-23 | Cetus Corporation | Stable high copy number plasmids |
| US4401761A (en) * | 1981-01-26 | 1983-08-30 | The Upjohn Company | Process for stabilizing plasmids by deletion of DNA |
-
1989
- 1989-12-18 DK DK639689A patent/DK639689D0/da not_active Application Discontinuation
-
1990
- 1990-12-18 EP EP91901587A patent/EP0506780B2/de not_active Expired - Lifetime
- 1990-12-18 ES ES91901587T patent/ES2064077T5/es not_active Expired - Lifetime
- 1990-12-18 CA CA002070676A patent/CA2070676A1/en not_active Abandoned
- 1990-12-18 JP JP50181391A patent/JP3162707B2/ja not_active Expired - Lifetime
- 1990-12-18 DK DK91901587T patent/DK0506780T4/da active
- 1990-12-18 DE DE69013520T patent/DE69013520T3/de not_active Expired - Lifetime
- 1990-12-18 AT AT91901587T patent/ATE113077T1/de not_active IP Right Cessation
- 1990-12-18 WO PCT/DK1990/000332 patent/WO1991009129A1/en not_active Ceased
- 1990-12-18 AU AU70312/91A patent/AU643741B2/en not_active Expired
-
1992
- 1992-06-17 FI FI922812A patent/FI112502B/fi active
- 1992-06-17 NO NO92922381A patent/NO922381L/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DK0506780T4 (da) | 2004-04-26 |
| FI922812A0 (fi) | 1992-06-17 |
| JPH05502162A (ja) | 1993-04-22 |
| FI112502B (fi) | 2003-12-15 |
| EP0506780A1 (de) | 1992-10-07 |
| AU643741B2 (en) | 1993-11-25 |
| DK0506780T3 (da) | 1995-04-18 |
| NO922381L (no) | 1992-08-17 |
| ES2064077T3 (es) | 1995-01-16 |
| DK639689D0 (da) | 1989-12-18 |
| EP0506780B1 (de) | 1994-10-19 |
| DE69013520D1 (de) | 1994-11-24 |
| AU7031291A (en) | 1991-07-18 |
| EP0506780B2 (de) | 2004-01-02 |
| JP3162707B2 (ja) | 2001-05-08 |
| ES2064077T5 (es) | 2004-09-01 |
| DE69013520T2 (de) | 1995-05-04 |
| WO1991009129A1 (en) | 1991-06-27 |
| FI922812L (fi) | 1992-06-17 |
| CA2070676A1 (en) | 1991-06-19 |
| DE69013520T3 (de) | 2004-09-23 |
| NO922381D0 (no) | 1992-06-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 0506780 Country of ref document: EP |
|
| EELA | Cancelled due to lapse of time |