ATE114714T1 - Verfahren zur kristallisation von subtilisin. - Google Patents

Verfahren zur kristallisation von subtilisin.

Info

Publication number
ATE114714T1
ATE114714T1 AT89903871T AT89903871T ATE114714T1 AT E114714 T1 ATE114714 T1 AT E114714T1 AT 89903871 T AT89903871 T AT 89903871T AT 89903871 T AT89903871 T AT 89903871T AT E114714 T1 ATE114714 T1 AT E114714T1
Authority
AT
Austria
Prior art keywords
subtilisin
crystallization
solution
concentrate
concentrated
Prior art date
Application number
AT89903871T
Other languages
English (en)
Inventor
Todd Becker
Bryan Lawlis Virgil Jr
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE114714T1 publication Critical patent/ATE114714T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT89903871T 1988-03-18 1989-03-17 Verfahren zur kristallisation von subtilisin. ATE114714T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US16999088A 1988-03-18 1988-03-18

Publications (1)

Publication Number Publication Date
ATE114714T1 true ATE114714T1 (de) 1994-12-15

Family

ID=22618060

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89903871T ATE114714T1 (de) 1988-03-18 1989-03-17 Verfahren zur kristallisation von subtilisin.

Country Status (5)

Country Link
EP (1) EP0404817B1 (de)
JP (1) JP2886921B2 (de)
AT (1) ATE114714T1 (de)
DE (1) DE68919688T2 (de)
WO (1) WO1989008703A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69004101T2 (de) * 1989-12-21 1994-03-24 Novonordisk As Verfahren zur kristallisation von enzymen.
US5837513A (en) * 1989-12-21 1998-11-17 Nielsen; Niels-Viktor Method for crystallization of enzymes
WO1991009928A1 (en) * 1989-12-21 1991-07-11 Novo Nordisk A/S Method for production of a clear, liquid detergent
WO1991009941A1 (en) * 1989-12-21 1991-07-11 Novo Nordisk A/S Enzyme containing preparation and detergent containing such preparation
US5281526A (en) * 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
NZ295735A (en) * 1994-12-28 1999-06-29 Genencor International Indiana Method for stabilizing enzymes in liquid compositions comprising forming an enzyme in an insoluble form and combining with an agent
US6207437B1 (en) 1996-03-11 2001-03-27 Genencor International, Inc. Crystalline protease and method for producing same
CA2361507C (en) * 1999-03-05 2014-05-06 Genencor International, Inc. Method for rapidly obtaining crystals with desirable morphologies

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5739782A (en) * 1980-08-22 1982-03-05 Toyo Soda Mfg Co Ltd Preparation of saturated enzyme solution

Also Published As

Publication number Publication date
DE68919688D1 (de) 1995-01-12
EP0404817A1 (de) 1991-01-02
WO1989008703A1 (en) 1989-09-21
EP0404817B1 (de) 1994-11-30
JP2886921B2 (ja) 1999-04-26
JPH03504320A (ja) 1991-09-26
DE68919688T2 (de) 1995-04-13

Similar Documents

Publication Publication Date Title
ATE148425T1 (de) Verfahren zur herstellung von natriumchlorid
KR890700590A (ko) 폴린산의 분리방법
DE68924489D1 (de) Salzerzeugnis und verfahren zur herstellung.
KR860000291A (ko) 결정성 칼슘 슈도모네이트의 제법
DK0506866T3 (da) Fremgangsmåde til krystallisation af enzymer
ATE76098T1 (de) Stabiles glukose-isomerase-konzentrat und verfahren zu dessen herstellung.
ATE232508T1 (de) Umweltschonendes verfahren zur herstellung von natriumchloridkristallen
PT86400A (en) Process for preparing aqueous solutions containing a mixture of hysozyme hydrochloride and dipotassium glycyrrhizate
OA09227A (fr) "Sels de céphalosporine, mélanges contenant ces sels et procédé de préparation du sel d'addition d'acide sulfurique".
ATE114714T1 (de) Verfahren zur kristallisation von subtilisin.
FI101794B1 (fi) Menetelmä kidevedettömän kiteisen laktuloosin valmistamiseksi
MA20210A1 (fr) Procede de preparation de nouveaux derives de l'acide 4 - phenyl 4-oxo buten-2-oique.
ATE187735T1 (de) Thermodynamisch stabile kristallform von 4''- deoxy-4''-epi-methylamino avermectin bia/bib benzoesauresalz und verfahren zu seiner herstellung
DE69114202D1 (de) Verfahren zur Herstellung von S(+)-6-Methoxy-alpha-methyl-2-naphthalin-Essigsäure.
SU1837586A1 (ru) Способ получения хлоридов калия и натрия из сильвинита
FR2574790B1 (fr) Procede de preparation d'acide glutamique a l'etat cristallise de preference sous forme de son sel sodique ou msg; acide glutamique, msg, sels et concentrat organique obtenus par ce procede
DE3677749D1 (de) Verfahren zur herstellung einer waessrigen natriumchloridloesung und verfahren zur erzeugung von natriumbikarbonat.
GB2009724A (en) Process for purifying crystalline potassium chloride
UST995006I4 (en) Method of removal of boron from aqueous solutions
DE68907146D1 (de) Verfahren zur reinigung von d-hydroxyphenoxypropionsaeure.
SE8404185D0 (sv) Procede pour la preparation d'une solution aqueuse de chlorure de sodium et procede de fabrication de bicarbonate de sodium
KR910011161A (ko) 글루타민산 칼슘염의 제조방법
DE69017145D1 (de) Verfahren zur Trennung von R und S-2, 2-R1,R2-1,3-Dioxolan-4-Carbonsäure.
MX167394B (es) Procedimiento para producir cloruro de sodio de grado elevado
SU1579088A1 (ru) Способ выращивания затравочных кристаллов пентабората калия

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee