ATE124703T1 - Verfahren zur trennung von proteinen. - Google Patents

Verfahren zur trennung von proteinen.

Info

Publication number
ATE124703T1
ATE124703T1 AT87890061T AT87890061T ATE124703T1 AT E124703 T1 ATE124703 T1 AT E124703T1 AT 87890061 T AT87890061 T AT 87890061T AT 87890061 T AT87890061 T AT 87890061T AT E124703 T1 ATE124703 T1 AT E124703T1
Authority
AT
Austria
Prior art keywords
value
ionic strength
gradually changing
separating proteins
maintaining
Prior art date
Application number
AT87890061T
Other languages
English (en)
Inventor
Thomas Prof Dr Vukovich
Original Assignee
Thomas Prof Dr Vukovich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thomas Prof Dr Vukovich filed Critical Thomas Prof Dr Vukovich
Application granted granted Critical
Publication of ATE124703T1 publication Critical patent/ATE124703T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood
    • Y10S530/83—Plasma; serum
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood
    • Y10S530/83—Plasma; serum
    • Y10S530/831—Cohn fractions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT87890061T 1986-03-27 1987-03-26 Verfahren zur trennung von proteinen. ATE124703T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT0082786A AT399095B (de) 1986-03-27 1986-03-27 Verfahren zur auftrennung von proteinen mittels gradientenelution und vorrichtung zur durchführung des verfahrens

Publications (1)

Publication Number Publication Date
ATE124703T1 true ATE124703T1 (de) 1995-07-15

Family

ID=3500677

Family Applications (2)

Application Number Title Priority Date Filing Date
AT0082786A AT399095B (de) 1986-03-27 1986-03-27 Verfahren zur auftrennung von proteinen mittels gradientenelution und vorrichtung zur durchführung des verfahrens
AT87890061T ATE124703T1 (de) 1986-03-27 1987-03-26 Verfahren zur trennung von proteinen.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT0082786A AT399095B (de) 1986-03-27 1986-03-27 Verfahren zur auftrennung von proteinen mittels gradientenelution und vorrichtung zur durchführung des verfahrens

Country Status (7)

Country Link
US (1) US5112949A (de)
EP (1) EP0239565B1 (de)
JP (1) JP2511449B2 (de)
AT (2) AT399095B (de)
AU (1) AU596159B2 (de)
CA (1) CA1327674C (de)
DE (1) DE3751389T2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4664913A (en) * 1982-05-24 1987-05-12 Xoma Corporation Method for treating plasma for transfusion
US4810391A (en) * 1987-11-06 1989-03-07 Bio-Rad Laboratories, Inc. Separation of hemoglobin A2 from hemoglobin mixture
ES2064412T3 (es) * 1988-10-26 1995-02-01 American Cyanamid Co Procedimiento para reducir el contenido de aglomerado de materiales tipo hormona del crecimiento.
US5110913A (en) * 1990-05-25 1992-05-05 Miles Inc. Antibody purification method
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
EP0585504A1 (de) * 1992-09-04 1994-03-09 Pentapharm A.G. Phospholipidabhängiger Prothrombinaktivator und seine Anwendung in Lupusantikoagulanttesten
DK114392D0 (da) * 1992-09-17 1992-09-17 Kem En Tec As Isolering af biomolekyler
ZA983496B (en) * 1997-04-28 1999-10-25 Lilly Co Eli Methods for processing activated protein C.
AU769144B2 (en) * 1997-04-28 2004-01-15 Eli Lilly And Company Improved methods for processing activated protein C
US6630137B1 (en) * 1997-04-28 2003-10-07 Eli Lilly And Company Activated protein C formulations
AT408613B (de) * 1998-06-17 2002-01-25 Immuno Ag Pharmazeutisches faktor vii-präparat
ATE277073T1 (de) * 1998-06-23 2004-10-15 Shanbrom Tech Llc Verfahren und vorrichtung zur herstellung von gereinigten plasmaproteinen
US7204981B2 (en) * 2000-03-28 2007-04-17 Eli Lilly And Company Methods of treating diseases with activated protein C
EP1289543A2 (de) 2000-05-24 2003-03-12 Eli Lilly And Company Formulierungen und verfahren zur behandlung von hyperkoagulablen zustäanden
US20050143283A1 (en) * 2002-03-08 2005-06-30 Eli Lilly And Company Activated protein c formulations
WO2005040787A1 (en) * 2003-10-17 2005-05-06 Ge Healthcare Bio-Sciences Ab Chromatography system, method and software for the separation of biomolecules
US20060057275A1 (en) * 2004-09-16 2006-03-16 Shaowen Wu Process for the preparation of glycinin-rich and beta-conglycinin-rich protein fractions
DK2269055T3 (da) * 2008-04-21 2012-01-23 Ge Healthcare Bio Sciences Ab Fremstilling af væskeblandinger
EP2480943B1 (de) * 2009-09-25 2017-07-12 GE Healthcare Bio-Sciences AB Verfahren und system zur herstellung flüssiger mischungen
US12227734B2 (en) 2011-03-08 2025-02-18 University Of Maryland, Baltimore County Microscale bioprocessing system and method for protein manufacturing from human blood
CA2956924C (en) * 2014-07-31 2023-08-01 Govind Rao Microscale bioprocessing system and method for protein manufacturing from human blood

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE392038B (sv) * 1971-09-08 1977-03-14 Kabi Ab Forfarande for isolering av antitrombin ur blod eller blodprodukter
CS162953B1 (de) * 1972-05-10 1975-07-31
AT336182B (de) * 1972-12-13 1977-04-25 Cutter Lab Verfahren zur herstellung eines prothrombinkomplexes
FR2210396A1 (en) * 1972-12-19 1974-07-12 Cutter Lab Blood coagulation complex - by extraction of plasma with sephadex
DE3172210D1 (en) * 1980-05-27 1985-10-17 Miles Lab Blood coagulation promoting product and process of preparing same
US4447416A (en) * 1982-04-28 1984-05-08 American National Red Cross Plasma protein concentrates of reduced thrombogenicity and their use in clinical replacement therapy
FR2543448A1 (fr) * 1983-04-01 1984-10-05 Rhone Poulenc Spec Chim Procede de fractionnement du plasma
GB8327860D0 (en) * 1983-10-18 1983-11-16 Fujisawa Pharmaceutical Co Monoclonal antiprotein c antibody
GB8403473D0 (en) * 1984-02-09 1984-03-14 Special Trustees For St Thomas Purification of factor viii
US4721572A (en) * 1985-07-12 1988-01-26 Miles Laboratories, Inc. Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices
US4770781A (en) * 1986-03-03 1988-09-13 Merck & Co., Inc. Purification of human interleukin-1 species

Also Published As

Publication number Publication date
DE3751389T2 (de) 1996-03-21
EP0239565A2 (de) 1987-09-30
JP2511449B2 (ja) 1996-06-26
CA1327674C (en) 1994-03-08
AU7067287A (en) 1987-10-01
DE3751389D1 (de) 1995-08-10
AT399095B (de) 1995-03-27
US5112949A (en) 1992-05-12
ATA82786A (de) 1989-09-15
JPS62246593A (ja) 1987-10-27
EP0239565B1 (de) 1995-07-05
AU596159B2 (en) 1990-04-26
EP0239565A3 (en) 1989-07-26

Similar Documents

Publication Publication Date Title
DE3751389D1 (de) Verfahren zur Trennung von Proteinen.
NO176570C (no) Proteinmateriale, inhibin, fremgangsmåte for renfremstilling av dette, antistoff mot inhibin, ikke-terapeutiske blandinger inneholdende inhibin/antistoff samt anvendelse for diagnostiske og analytiske formål
DE3484179D1 (de) Verfahren zur reinigung von faktor viii-c.
ES2106742T3 (es) Procedimiento de purificacion de proteinas.
DE3585147D1 (de) Verfahren zur reinigung von proteinen.
IL79972A0 (en) Protein recovery
DE3669722D1 (de) Verfahren zur vernetzung von poroesen polysaccharid-gelen.
DE3865963D1 (de) Elektrophoretisches gel hoher aufloesung sowie methode zur abscheidung von serumproteinen.
ATE299889T1 (de) Neues verfahren zur trennung von proteinen unter verwendung eines ca++ enthaltenden elutionsmittels
IL88118A (en) Ion-exchange chromatographic method of purifying proteins
DE69113608D1 (de) Verfahren zum Trennen von glykosiliertem Hämoglobin HBA1C durch Agarosegelelektroforese.
GR852288B (de)
EP0087686A3 (de) Homogenes menschliches immunes Interferon und Verfahren zur Herstellung desselben
ATE119543T1 (de) Produktion von proteinen in aktiver form.
DE3485711D1 (de) Immunoadsorbens und verfahren zur zurueckgewinnung von vitamin-k-abhaengigen proteinen mittels dieses adsorbens.
DE3788170D1 (de) Methode zur Reinigung und Isolierung von zwei ionischen Formen von Somatomedin.
Davis et al. Electrophoretic characteristics of macroamylasemic serum
ES2080804T3 (es) Separacion de anticuerpos anti-metal quelato.
ATE341617T1 (de) Prozess zur reinigung von vitamin k abhängigen koagulationsfaktoren durch chromatographie
AT380772B (de) Verfahren zur gewinnung von proteinen
ATE175214T1 (de) Methode zur trennung von glykosylierten und unglykosylierten proteinen
DE3785852D1 (de) Verfahren zur chromatographischen trennung von metallionen.
CS674688A1 (en) Method of stobadin-dipalmitane's as well as toluidine's trace quantities parallel determination in these intermediate products by means of silica gels high-effective liquid chromatigraphy with chemically bound octyl groups
IT1248999B (it) Marcatura di dna doppia elica mediante transaminazione
DD215319A1 (de) Verfahren zur anreicherung von interferonen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties