ATE124724T1 - Penicillin-g-acylase, dafür kodierendes gen und verfahren zur herstellung von diesem enzym. - Google Patents

Penicillin-g-acylase, dafür kodierendes gen und verfahren zur herstellung von diesem enzym.

Info

Publication number
ATE124724T1
ATE124724T1 AT91200929T AT91200929T ATE124724T1 AT E124724 T1 ATE124724 T1 AT E124724T1 AT 91200929 T AT91200929 T AT 91200929T AT 91200929 T AT91200929 T AT 91200929T AT E124724 T1 ATE124724 T1 AT E124724T1
Authority
AT
Austria
Prior art keywords
enzyme
acylase
penicillin
producing
gene
Prior art date
Application number
AT91200929T
Other languages
English (en)
Inventor
Wilhelmus Johannes Quax
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Application granted granted Critical
Publication of ATE124724T1 publication Critical patent/ATE124724T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01011—Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/829—Alcaligenes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT91200929T 1990-04-18 1991-04-18 Penicillin-g-acylase, dafür kodierendes gen und verfahren zur herstellung von diesem enzym. ATE124724T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP90200962 1990-04-18
EP90203463 1990-12-20

Publications (1)

Publication Number Publication Date
ATE124724T1 true ATE124724T1 (de) 1995-07-15

Family

ID=26125836

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91200929T ATE124724T1 (de) 1990-04-18 1991-04-18 Penicillin-g-acylase, dafür kodierendes gen und verfahren zur herstellung von diesem enzym.

Country Status (10)

Country Link
US (2) US5168048A (de)
EP (1) EP0453047B1 (de)
JP (1) JPH04228073A (de)
KR (1) KR0152667B1 (de)
AT (1) ATE124724T1 (de)
DE (1) DE69110952T2 (de)
DK (1) DK0453047T3 (de)
ES (1) ES2077151T3 (de)
IE (1) IE68078B1 (de)
PT (1) PT97397B (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE167520T1 (de) * 1990-04-18 1998-07-15 Gist Brocades Nv Mutierte beta-lactamacylasegene
DE69421653D1 (de) * 1993-08-05 1999-12-23 Dsm Nv Neue Anwendung von Alcaligenes faecalis Penicillin G Acylase
AU3346295A (en) * 1994-08-12 1996-03-07 Gist-Brocades B.V. Mutated penicillin g acylase genes
US5516679A (en) * 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
US5919680A (en) * 1995-11-27 1999-07-06 Isis Innovation Limited Process for the production of SSC's via expandase activity on penicillin G
CA2270400C (en) 1996-11-05 2010-09-28 Bristol-Myers Squibb Company Mutant penicillin g acylases
US6437113B1 (en) 1998-03-05 2002-08-20 Tularik Inc. Suppressors of death domains
AU4436900A (en) * 1999-04-29 2000-11-17 Dsm N.V. Expression cassette for efficient production of a protein
US20030044884A1 (en) * 2000-10-11 2003-03-06 Usher John J. Synthesis of beta-lactam antibacterials using soluble side chain esters and enzyme acylase
US6699699B2 (en) 2002-03-26 2004-03-02 Synmax Biochemical Co., Ltd. Mutated penicillin expandases
WO2003104446A1 (ja) * 2002-06-06 2003-12-18 鐘淵化学工業株式会社 新規アシラーゼ遺伝子
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
CZ200782A3 (cs) * 2007-01-31 2009-05-27 Mikrobiologický ústav AV CR, v.v.i. Sekvence nukleotidu o velikosti 2646 bp, kódující penicilinacylázu, nové konstrukty rekombinantní nukleové kyseliny a rekombinantní mikroorganismy nesoucí tuto sekvenci
TW201022445A (en) * 2008-10-09 2010-06-16 Dsm Ip Assets Bv Method for preparing ε-caprolactam from N-acyl-6-aminocaproic acid
CA2894597C (en) 2012-12-14 2020-09-22 Saban Ventures Pty Limited Disinfectant solution comprising peroxyacetic acid, hydrogen peroxide and a carbonate buffer
US9789216B2 (en) 2012-12-14 2017-10-17 Saban Ventures Pty Limited Synergistic disinfection enhancement
CN113913416B (zh) * 2021-10-14 2022-03-22 巢湖学院 一种粪产碱杆菌青霉素g酰化酶的制备工艺
CN115418331B (zh) * 2022-08-29 2024-09-10 新发药业有限公司 一种产青霉素g酰基转移酶自诱导培养基及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3239427A (en) * 1959-10-12 1966-03-08 Pfizer & Co C Production of 6-aminopenicillanic acid
US3272715A (en) * 1962-07-17 1966-09-13 American Home Prod Method for producing and recovering 6-aminopenicillanic acid
BE790075A (fr) * 1971-10-14 1973-04-13 Bayer Ag Procede de preparation de penicillinacylase cristalline pure etproduit obtenu
DE2732301C3 (de) * 1977-07-16 1980-12-11 Roehm Gmbh, 6100 Darmstadt Verfahren zur Herstellung von stabilisierten trägergebundenen Proteinen
DE3330003A1 (de) * 1982-10-21 1984-10-31 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Dna-sequenz und dna-strukturen und sie verwendendes verfahren zur herstellung von penicillin-acylase
JPS6066981A (ja) * 1983-09-12 1985-04-17 アメリカン・ホ−ム・プロダクツ・コ−ポレイシヨン 不溶化ペニシリンアミダ−ゼおよびその製法
DE58907667D1 (de) * 1988-04-08 1994-06-23 Biochemie Gmbh Gen und Genprodukt zur Herstellung von beta-lactamverbindungen.
DE3909018A1 (de) * 1989-03-18 1990-09-20 Roehm Gmbh Protein-reinigungsverfahren

Also Published As

Publication number Publication date
DE69110952D1 (de) 1995-08-10
PT97397B (pt) 1998-08-31
US5168048A (en) 1992-12-01
JPH04228073A (ja) 1992-08-18
KR0152667B1 (ko) 1998-10-01
EP0453047A1 (de) 1991-10-23
IE68078B1 (en) 1996-05-15
DK0453047T3 (da) 1995-09-11
ES2077151T3 (es) 1995-11-16
EP0453047B1 (de) 1995-07-05
IE911294A1 (en) 1991-10-23
PT97397A (pt) 1992-01-31
DE69110952T2 (de) 1995-11-16
KR910018550A (ko) 1991-11-30
US5695978A (en) 1997-12-09

Similar Documents

Publication Publication Date Title
ATE124724T1 (de) Penicillin-g-acylase, dafür kodierendes gen und verfahren zur herstellung von diesem enzym.
ATE417094T1 (de) Verfahren zur herstellung von l-aminosäure
ATE162551T1 (de) Verfahren zur herstellung von dihomo-gamma- linolensäure und das in dieser enthaltende lipid
DE69721292D1 (de) Enzymatisches verfahren zur herstellung von ascorbinsäure,2-keto-l-gulonsäure und 2-keto-l-gulonsäure-estern
DE69019967D1 (de) Enzymatisches verfahren zur herstellung von 7-aminocephalosporansäure.
ATE106451T1 (de) Fermentationsverfahren zur herstellung von 2-keto-l-gulonsäure.
DE3888804D1 (de) Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase.
ATE112574T1 (de) Igg-bindendes protein h, kodierendes gen dafür und verfahren zu seiner herstellung.
ATE171728T1 (de) Trypsintyp-enzyme, dafür kodierende nukleinsäuresequenz und verfahren zur herstellung des enzymes
DE68926893D1 (de) Verfahren zur Herstellung von clavulanic Säure
ATE118817T1 (de) Verfahren zur herstellung von xbai- restriktionsendonuklease und -methylase.
ATE95836T1 (de) Zum abbau von glycosaminoglycan faehige enzymzusammensetzung und verfahren zur herstellung dieser zusammensetzung.
ATE154393T1 (de) Verfahren zur herstellung von trans-l-hydroxy- prolin
ATE106448T1 (de) Verfahren zur herstellung von hinpi- restriktionsendonuklease und -methylase.
ATE103329T1 (de) Verfahren zur herstellung von hgiairestriktionsendonuklease und -methylase.
ATE274063T1 (de) Dns, die für ein rekombinantes enzym zur umwandlung von maltose in trehalose kodiert
DE68913055D1 (de) Verfahren zur Herstellung von AseI-Restriktionsendonuklease und -methylase.
ATE76662T1 (de) Enzymatisches verfahren zur herstellung von 3substituierten cephalosporinen.
ATE196927T1 (de) Verfahren zur herstellung von proteaseinhibitoren
ATE105860T1 (de) Gen und genprodukt zur herstellung von beta- lactamverbindungen.
ATE171214T1 (de) Verfahren zur herstellung einer alpha- acetomilchsäure
DE3853143D1 (de) Verfahren zur Herstellung von AccI-Restriktionsendonuklease und -methylase.
DE59208669D1 (de) Verfahren zur Herstellung von MA
DE3785395D1 (de) Verfahren zur herstellung von difluorbenzamid.
DE69422298D1 (de) Enzym und dessen verwendung zur herstellung von (s)-pipecolinsäure

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EEIH Change in the person of patent owner
EELA Cancelled due to lapse of time