ATE127845T1 - Künstlicher promoter zur proteine expression in hefe. - Google Patents

Künstlicher promoter zur proteine expression in hefe.

Info

Publication number
ATE127845T1
ATE127845T1 AT90403797T AT90403797T ATE127845T1 AT E127845 T1 ATE127845 T1 AT E127845T1 AT 90403797 T AT90403797 T AT 90403797T AT 90403797 T AT90403797 T AT 90403797T AT E127845 T1 ATE127845 T1 AT E127845T1
Authority
AT
Austria
Prior art keywords
yeast
sequence
protein expression
artificial promoter
promoter
Prior art date
Application number
AT90403797T
Other languages
English (en)
Inventor
Pascal Leplatois
Gerard Loison
Bernard Pessegue
David Shire
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Application granted granted Critical
Publication of ATE127845T1 publication Critical patent/ATE127845T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5421—IL-8
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C12N9/0046—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7) with oxygen as acceptor (1.7.3)
    • C12N9/0048—Uricase (1.7.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/03—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with oxygen as acceptor (1.7.3)
    • C12Y107/03003—Factor-independent urate hydroxylase (1.7.3.3), i.e. uricase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT90403797T 1989-12-29 1990-12-28 Künstlicher promoter zur proteine expression in hefe. ATE127845T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8917467A FR2656531B1 (fr) 1989-12-29 1989-12-29 Promoteur artificiel pour l'expression de proteines dans le levure.

Publications (1)

Publication Number Publication Date
ATE127845T1 true ATE127845T1 (de) 1995-09-15

Family

ID=9389162

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90403797T ATE127845T1 (de) 1989-12-29 1990-12-28 Künstlicher promoter zur proteine expression in hefe.

Country Status (14)

Country Link
EP (1) EP0435776B1 (de)
JP (1) JP2723358B2 (de)
AT (1) ATE127845T1 (de)
BR (1) BR1100340A (de)
CA (1) CA2048676C (de)
DE (1) DE69022380T2 (de)
DK (1) DK0435776T3 (de)
ES (1) ES2080816T3 (de)
FR (1) FR2656531B1 (de)
GR (1) GR3018220T3 (de)
IE (1) IE71208B1 (de)
LV (1) LV11748B (de)
PT (1) PT96388B (de)
WO (1) WO1991009956A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992006196A1 (en) * 1990-09-28 1992-04-16 Cetus Oncology Corporation Gro genes, proteins, and uses thereof
US6110707A (en) * 1996-01-19 2000-08-29 Board Of Regents, The University Of Texas System Recombinant expression of proteins from secretory cell lines
CL2008002053A1 (es) 2007-07-17 2009-05-22 Hoffmann La Roche Metodo para la purificacion de una eritropoyetina monopeguilada (epompeg) que consiste en proporcionar una solucion que contiene eritropoyetina mono, poli y no peguilada y hacerla pasar por dos pasos de cromatografia de intercambio cationico y metodo para producir epo mpeg que incluye metodo de purificacion.
AR067536A1 (es) 2007-07-17 2009-10-14 Hoffmann La Roche Metodo para obtener una eritropoyetina mono-pegilada en una forma sustancialmente homogenea
KR20220064647A (ko) 2020-11-12 2022-05-19 에스케이이노베이션 주식회사 내산성 효모 유전자 기반 합성 프로모터

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR6301M (de) * 1967-03-29 1968-09-09
ATE102250T1 (de) * 1984-05-11 1994-03-15 Chiron Corp Erhoehte hefetranskription unter verwendung einer hybridkonstruktion der promotorregion.
EP0174585A3 (de) * 1984-09-14 1987-12-23 Miles Laboratories, Inc. Promotoren und deren Anwendung zur Expression von nichtfusierten prokaryontischen oder enkaryontischen Proteinen in Hefe
FR2607517B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
CA1304020C (en) * 1987-03-23 1992-06-23 Meher H. Irani High level expression in yeast
JPS6451089A (en) * 1987-08-21 1989-02-27 Yamasa Shoyu Kk Polynucleotide fragment

Also Published As

Publication number Publication date
DE69022380D1 (de) 1995-10-19
IE71208B1 (en) 1997-02-12
CA2048676A1 (en) 1991-06-30
JPH07507439A (ja) 1995-08-24
ES2080816T3 (es) 1996-02-16
LV11748B (en) 1997-08-20
LV11748A (lv) 1997-04-20
CA2048676C (en) 2000-04-04
PT96388A (pt) 1991-10-15
FR2656531B1 (fr) 1992-04-24
BR1100340A (pt) 2000-03-14
FR2656531A1 (fr) 1991-07-05
GR3018220T3 (en) 1996-02-29
JP2723358B2 (ja) 1998-03-09
DE69022380T2 (de) 1996-03-28
WO1991009956A1 (fr) 1991-07-11
EP0435776B1 (de) 1995-09-13
PT96388B (pt) 1998-06-30
EP0435776A1 (de) 1991-07-03
DK0435776T3 (da) 1996-02-05
IE904706A1 (en) 1991-07-17

Similar Documents

Publication Publication Date Title
ATE187775T1 (de) Verfahren zur herstellung von menschlichem serumalbumin in hefe
DK221589D0 (da) Fremgangsmaade til at udtrykke ikke-gaerprotein i en gaervaert og transformeret gaervaert
ATE120490T1 (de) Stressproteine aus pilzen.
DE3584149D1 (de) Verfahren zur herstellung von alkohol.
ES2109238T3 (es) Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
DE59207351D1 (de) Verbesserte Aktivierung von rekombinanten Proteinen
DE3650135D1 (de) Proteingewinnung.
SE8801701D0 (sv) Production technology based on enzymic method
DE69022380D1 (de) Künstlicher Promoter zur Proteine Expression in Hefe.
DE68921354D1 (de) Strukturgen für membrangebundene Aldehyd-Dehydrogenase, Plasmid, das es enthält, mit diesem Plasmid transformierte Essigsäurebakterien und Essigsäuregärung mittels dieser Transformanten.
WO2002044337A3 (en) Wooden leg gene, promoter and uses thereof
DK620084A (da) Fremgangsmaade til fremstilling af ethanol
FI910795A0 (fi) 0-glykocylerad ifg-1.
EP1576095A3 (de) Fettalkoholoxidase-gene und -proteine aus candida tropicalis und damit verbundene verfahren
DE3782560D1 (de) Hefestaemme zur expression fremder gene.
DE60302776D1 (de) Verfahren zur rekombinanten Herstellung von Polypeptiden
KR970027317A (ko) 이종 단백질을 생물공학적으로 고수율로 생산하기 위한 효모 알콜 데하이드 로게나제 이소엔자임 ii(adh ii) 프로모터 시스템의 사용방법.
DE3877446D1 (de) Verfahren zur synthese von glutathion in hefen.
IT1299201B1 (it) Fibroblasti modificati geneticamente e loro uso
ATE510922T1 (de) Induzierbare promotoren
DK391185A (da) Rekombinant saccharomyces
ATE264917T1 (de) Integrationsvektoren und verfahren zur herstellung rekombinanter proteine in pilzen
WO2001060839A3 (en) Method for refolding recombinant endostatin
ES8506083A1 (es) Procedimiento para preparar l-aminoacido-oxidasas
FR2285080A1 (fr) Procede d'obtention de proteines d'organismes unicellulaires a partir de substrats amylaces

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time