ES2109238T3 - Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion. - Google Patents

Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.

Info

Publication number
ES2109238T3
ES2109238T3 ES90911878T ES90911878T ES2109238T3 ES 2109238 T3 ES2109238 T3 ES 2109238T3 ES 90911878 T ES90911878 T ES 90911878T ES 90911878 T ES90911878 T ES 90911878T ES 2109238 T3 ES2109238 T3 ES 2109238T3
Authority
ES
Spain
Prior art keywords
protein
multicopy integration
integration
expression
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90911878T
Other languages
English (en)
Inventor
Marco Luigi Frederic Giuseppin
Maria Teresa Santos Lopes
Roelf Johannes Planta
Johannes Maria Antoni Verbakel
Cornelis Theodorus Verrips
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB898915659A external-priority patent/GB8915659D0/en
Application filed by Unilever NV filed Critical Unilever NV
Application granted granted Critical
Publication of ES2109238T3 publication Critical patent/ES2109238T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE PRESENTA UN PROCESO PARA LA PREPARACION DE UNA PROTEINA A PARTIR DE UN EUCARIOTE TRANSFORMADO POR UNA INTEGRACION MULTICOPIA DE UN VECTOR DE EXPRESION EN LA GENOMA DE UNA LEVADURA, COMO POR EJEMPLO SACCHAROMYCES, HANSENULA Y KLUYVEROMYCES, O DE UN MOHO COMO POR EJEMPLO ASPERGILLUS, RHIZOPUS Y TRICHODERMA, CONTENIENDO DICHO VECTOR DE EXPRESION TANTO UN "GEN EXPRESIBLE" QUE CODIFICA DICHA PROTEINA COMO EL ASI LLAMADO "MARCADOR DE SELECCION DEFICIENTE NECESARIO PARA EL CRECIMIENTO DE LA LEVADURA O MOHO EN UN MEDIO ESPECIFICO", COMO POR EJEMPLO EL GEN LEU2D, TRP1D O URA3D , JUNTO CON UNA SECUENCIA DE ADN RIBOSOMAL, LO QUE DA COMO RESULTADO UNA INTEGRACION ALTA DE LA COPIA ESTABLE DE 100 A 300 COPIAS POR CELULA. ESTA INTEGRACION MULTICOPIA DA COMO RESULTADO UN AUMENTO DE LA PRODUCCION DE LA PROTEINA DESEADA, QUE PUEDE SER UNA (ALFA)-GALACTOSIDASA DE GUAR, UNA OXIDASA O UNA ENZIMA HIDROLITICA COMO POR EJEMPLO UNA LIPASA.
ES90911878T 1989-07-07 1990-07-09 Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion. Expired - Lifetime ES2109238T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898915659A GB8915659D0 (en) 1989-07-07 1989-07-07 Enzymes,their production and use
EP90201007 1990-04-20

Publications (1)

Publication Number Publication Date
ES2109238T3 true ES2109238T3 (es) 1998-01-16

Family

ID=26125849

Family Applications (2)

Application Number Title Priority Date Filing Date
ES90911878T Expired - Lifetime ES2109238T3 (es) 1989-07-07 1990-07-09 Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
ES97200631T Expired - Lifetime ES2150188T3 (es) 1989-07-07 1990-07-09 Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES97200631T Expired - Lifetime ES2150188T3 (es) 1989-07-07 1990-07-09 Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.

Country Status (10)

Country Link
US (1) US6090574A (es)
EP (2) EP0481008B1 (es)
JP (1) JP3273609B2 (es)
AT (2) ATE160176T1 (es)
CA (1) CA2063592C (es)
DE (2) DE69033633T2 (es)
DK (2) DK0778348T3 (es)
ES (2) ES2109238T3 (es)
IE (1) IE81016B1 (es)
WO (1) WO1991000920A2 (es)

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DE69031710T2 (de) 1998-03-05
US6090574A (en) 2000-07-18
EP0481008B1 (en) 1997-11-12
ATE160176T1 (de) 1997-11-15
DE69031710D1 (de) 1997-12-18
JPH05501949A (ja) 1993-04-15
WO1991000920A2 (en) 1991-01-24
EP0778348B1 (en) 2000-09-20
ES2150188T3 (es) 2000-11-16
CA2063592A1 (en) 1991-01-08
CA2063592C (en) 2003-01-21
EP0481008A1 (en) 1992-04-22
WO1991000920A3 (en) 1991-03-07
DK0778348T3 (da) 2000-12-04
IE81016B1 (en) 1999-09-22
EP0778348A1 (en) 1997-06-11
JP3273609B2 (ja) 2002-04-08
ATE196505T1 (de) 2000-10-15
DK0481008T3 (da) 1998-05-04
DE69033633T2 (de) 2001-05-03
IE902496A1 (en) 1991-02-13
DE69033633D1 (de) 2000-10-26

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