ATE134388T1 - Verfahren und reagenzkombination zur bestimmung von nukleotidsequenzen - Google Patents
Verfahren und reagenzkombination zur bestimmung von nukleotidsequenzenInfo
- Publication number
- ATE134388T1 ATE134388T1 AT89121895T AT89121895T ATE134388T1 AT E134388 T1 ATE134388 T1 AT E134388T1 AT 89121895 T AT89121895 T AT 89121895T AT 89121895 T AT89121895 T AT 89121895T AT E134388 T1 ATE134388 T1 AT E134388T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- target nucleic
- attachment moiety
- solid matrix
- acid polymer
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 230000003321 amplification Effects 0.000 abstract 3
- 239000011159 matrix material Substances 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 229920000642 polymer Polymers 0.000 abstract 3
- 239000007787 solid Substances 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 238000001712 DNA sequencing Methods 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 238000004925 denaturation Methods 0.000 abstract 1
- 230000036425 denaturation Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 239000000376 reactant Substances 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27764388A | 1988-11-29 | 1988-11-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE134388T1 true ATE134388T1 (de) | 1996-03-15 |
Family
ID=23061771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT89121895T ATE134388T1 (de) | 1988-11-29 | 1989-11-28 | Verfahren und reagenzkombination zur bestimmung von nukleotidsequenzen |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP0371437B1 (de) |
| JP (1) | JP2766003B2 (de) |
| AT (1) | ATE134388T1 (de) |
| AU (1) | AU623200B2 (de) |
| CA (1) | CA2004056C (de) |
| DE (1) | DE68925717T2 (de) |
| DK (1) | DK175170B1 (de) |
| ES (1) | ES2082766T3 (de) |
| FI (1) | FI97153C (de) |
| GR (1) | GR3019105T3 (de) |
| IL (1) | IL92474A (de) |
| NO (1) | NO176362C (de) |
| NZ (1) | NZ231576A (de) |
| PT (1) | PT92420B (de) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE8801070D0 (sv) * | 1988-03-23 | 1988-03-23 | Pharmacia Ab | Method for immobilizing a dna sequence on a solid support |
| DE3816934A1 (de) * | 1988-05-18 | 1989-11-23 | Ramalho Ortigao Jose Flavio | Neue sequenzierungsmethode fuer einzel- oder doppelstraengige nucleinsaeuren an fester phase |
| US5858652A (en) * | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| US5547839A (en) | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
| US6013431A (en) | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
| IL97222A (en) * | 1990-02-16 | 1995-08-31 | Orion Yhtymae Oy | Method and reagent for determining specific nucleotide variations |
| GB9017788D0 (en) * | 1990-08-14 | 1990-09-26 | Medical Res Council | Method for preparing,isolating and sequencing polynucleotides |
| US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
| WO1992020820A1 (en) * | 1991-05-15 | 1992-11-26 | Vanderbilt University | Method to determine metastatic potential of tumor cells |
| US5569582A (en) * | 1991-07-15 | 1996-10-29 | Institute Of Molecular Biology & Technology | Rapid amplification and detection of nucleic acids |
| AU660484B2 (en) * | 1991-07-24 | 1995-06-29 | University Partnerships Pty Ltd | Single step amplification and sequencing of nucleic acids |
| WO1993002212A1 (en) * | 1991-07-24 | 1993-02-04 | University Partnerships Pty. Ltd. | Single step amplification and sequencing of nucleic acids |
| DE4214112A1 (de) * | 1991-08-02 | 1993-02-04 | Europ Lab Molekularbiolog | Neues verfahren zur sequenzierung von nukleinsaeuren |
| GB9122060D0 (en) * | 1991-10-17 | 1991-11-27 | Dynal As | Method of sequencing double stranded dna |
| GB9207598D0 (en) * | 1992-04-03 | 1992-05-20 | Dynal As | Method of sequencing double stranded dna |
| GB9210176D0 (en) † | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Chemical method |
| US5843640A (en) * | 1992-06-19 | 1998-12-01 | Northwestern University | Method of simultaneously detecting amplified nucleic acid sequences and cellular antigens in cells |
| US6436635B1 (en) | 1992-11-06 | 2002-08-20 | Boston University | Solid phase sequencing of double-stranded nucleic acids |
| US5795714A (en) | 1992-11-06 | 1998-08-18 | Trustees Of Boston University | Method for replicating an array of nucleic acid probes |
| CA2158642A1 (en) * | 1993-03-19 | 1994-09-29 | Hubert Koster | Dna sequencing by mass spectrometry via exonuclease degradation |
| ES2078185B1 (es) * | 1994-05-06 | 1996-08-16 | Inia | Procedimiento para la tipificacion molecular de patogenos virales con genoma rna y de patogenos subvirales mediante sintesis aleatoria de cdna. |
| ATE220424T1 (de) * | 1994-05-28 | 2002-07-15 | Tepnel Medical Ltd | Herstellung von nukleinsaeurekopien |
| US7803529B1 (en) | 1995-04-11 | 2010-09-28 | Sequenom, Inc. | Solid phase sequencing of biopolymers |
| US6077664A (en) * | 1995-06-07 | 2000-06-20 | Promega Corporation | Thermophilic DNA polymerases from Thermotoga neapolitana |
| US6001645A (en) * | 1995-06-07 | 1999-12-14 | Promega Corporation | Thermophilic DNA polymerases from thermotoga neapolitana |
| SE9504099D0 (sv) | 1995-11-16 | 1995-11-16 | Pharmacia Biotech Ab | A method of sequencing |
| WO1997035033A1 (en) | 1996-03-19 | 1997-09-25 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
| US7381525B1 (en) | 1997-03-07 | 2008-06-03 | Clinical Micro Sensors, Inc. | AC/DC voltage apparatus for detection of nucleic acids |
| US6096273A (en) | 1996-11-05 | 2000-08-01 | Clinical Micro Sensors | Electrodes linked via conductive oligomers to nucleic acids |
| US6140053A (en) | 1996-11-06 | 2000-10-31 | Sequenom, Inc. | DNA sequencing by mass spectrometry via exonuclease degradation |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| WO2000063437A2 (en) | 1999-04-20 | 2000-10-26 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| AU779220B2 (en) | 1999-07-26 | 2005-01-13 | Clinical Micro Sensors, Inc. | Sequence determination of nucleic acids using electronic detection |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7211390B2 (en) | 1999-09-16 | 2007-05-01 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6518024B2 (en) | 1999-12-13 | 2003-02-11 | Motorola, Inc. | Electrochemical detection of single base extension |
| ATE412774T1 (de) | 2000-02-16 | 2008-11-15 | Illumina Inc | Parallele genotypisierung mehrerer patientenproben |
| AU2002314734B2 (en) | 2001-03-30 | 2007-08-30 | Ge Healthcare Bio-Sciences Ab | P450 single nucleotide polymorphism biochip analysis |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8507706D0 (en) * | 1985-03-25 | 1985-05-01 | Genetics Int Inc | Magnetic nucleic acid sequences |
| AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| SE8801070D0 (sv) * | 1988-03-23 | 1988-03-23 | Pharmacia Ab | Method for immobilizing a dna sequence on a solid support |
| NO165894C (no) * | 1988-05-24 | 1991-04-24 | Gunnar Paulsen | Analysemetode for gener. |
| US5817797A (en) * | 1988-06-01 | 1998-10-06 | The United States Of America As Represented By The Department Of Health And Human Services | Sequencing DNA; a modification of the polymerase chain reaction |
-
1989
- 1989-11-27 DK DK198905953A patent/DK175170B1/da not_active IP Right Cessation
- 1989-11-27 IL IL9247489A patent/IL92474A/en not_active IP Right Cessation
- 1989-11-28 DE DE68925717T patent/DE68925717T2/de not_active Expired - Fee Related
- 1989-11-28 FI FI895682A patent/FI97153C/fi active IP Right Grant
- 1989-11-28 JP JP1308817A patent/JP2766003B2/ja not_active Expired - Fee Related
- 1989-11-28 ES ES89121895T patent/ES2082766T3/es not_active Expired - Lifetime
- 1989-11-28 EP EP89121895A patent/EP0371437B1/de not_active Expired - Lifetime
- 1989-11-28 CA CA002004056A patent/CA2004056C/en not_active Expired - Fee Related
- 1989-11-28 PT PT92420A patent/PT92420B/pt not_active IP Right Cessation
- 1989-11-28 AT AT89121895T patent/ATE134388T1/de not_active IP Right Cessation
- 1989-11-28 NO NO894746A patent/NO176362C/no unknown
- 1989-11-29 AU AU45749/89A patent/AU623200B2/en not_active Ceased
- 1989-11-29 NZ NZ231576A patent/NZ231576A/en unknown
-
1996
- 1996-02-23 GR GR960400527T patent/GR3019105T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2004056C (en) | 2004-05-11 |
| GR3019105T3 (en) | 1996-05-31 |
| EP0371437B1 (de) | 1996-02-21 |
| DE68925717T2 (de) | 1996-07-04 |
| AU623200B2 (en) | 1992-05-07 |
| DK175170B1 (da) | 2004-06-21 |
| NO894746L (no) | 1990-05-30 |
| JPH02219600A (ja) | 1990-09-03 |
| AU4574989A (en) | 1990-06-07 |
| FI895682A0 (fi) | 1989-11-28 |
| PT92420B (pt) | 1995-08-09 |
| EP0371437A3 (de) | 1991-03-27 |
| IL92474A (en) | 1994-04-12 |
| NO176362C (no) | 1995-03-22 |
| NO894746D0 (no) | 1989-11-28 |
| NO176362B (no) | 1994-12-12 |
| CA2004056A1 (en) | 1990-05-29 |
| ES2082766T3 (es) | 1996-04-01 |
| DK595389A (da) | 1990-05-30 |
| FI97153B (fi) | 1996-07-15 |
| NZ231576A (en) | 1991-12-23 |
| IL92474A0 (en) | 1990-08-31 |
| PT92420A (pt) | 1990-05-31 |
| DE68925717D1 (de) | 1996-03-28 |
| EP0371437A2 (de) | 1990-06-06 |
| FI97153C (fi) | 1996-10-25 |
| JP2766003B2 (ja) | 1998-06-18 |
| DK595389D0 (da) | 1989-11-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EEIH | Change in the person of patent owner | ||
| EEIH | Change in the person of patent owner | ||
| REN | Ceased due to non-payment of the annual fee |