ATE143371T1 - Verfahren zur proteinreinigung - Google Patents

Verfahren zur proteinreinigung

Info

Publication number
ATE143371T1
ATE143371T1 AT90917408T AT90917408T ATE143371T1 AT E143371 T1 ATE143371 T1 AT E143371T1 AT 90917408 T AT90917408 T AT 90917408T AT 90917408 T AT90917408 T AT 90917408T AT E143371 T1 ATE143371 T1 AT E143371T1
Authority
AT
Austria
Prior art keywords
protein
pct
antibody
date
sec
Prior art date
Application number
AT90917408T
Other languages
English (en)
Inventor
Jan Markussen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE143371T1 publication Critical patent/ATE143371T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT90917408T 1989-11-20 1990-11-20 Verfahren zur proteinreinigung ATE143371T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK580789A DK580789D0 (da) 1989-11-20 1989-11-20 Fremgangsmaade til oprensning af polypeptider

Publications (1)

Publication Number Publication Date
ATE143371T1 true ATE143371T1 (de) 1996-10-15

Family

ID=8145390

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90917408T ATE143371T1 (de) 1989-11-20 1990-11-20 Verfahren zur proteinreinigung

Country Status (8)

Country Link
US (1) US5317092A (de)
EP (1) EP0502050B1 (de)
JP (1) JPH05503081A (de)
AT (1) ATE143371T1 (de)
AU (1) AU6750990A (de)
DE (1) DE69028711T2 (de)
DK (1) DK580789D0 (de)
WO (1) WO1991007428A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU707719B2 (en) * 1995-07-21 1999-07-15 Amgen, Inc. Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt
US20030105303A1 (en) * 1996-12-06 2003-06-05 Schering Corporation, A New Jersey Corporation Isolated mammalian monocyte cell genes; related reagents
CN1352685A (zh) * 1998-12-31 2002-06-05 先灵公司 单核细胞来源的核酸和相关的组合物及方法
NZ518263A (en) * 1999-10-14 2004-03-26 Gtc Biotherapeutics Inc Methods of producing a target molecule in a transgenic animal and purification of the target molecule
US20090068635A1 (en) * 2007-09-06 2009-03-12 Muerhoff Anthony S Indirectly labelled assay conjugates and methods of preparing and using same
AU2011343813B2 (en) 2010-12-15 2015-05-21 Takeda Pharmaceutical Company Limited Eluate collection using conductivity gradient
PE20191404A1 (es) 2017-01-27 2019-10-04 Ngm Biopharmaceuticals Inc Proteinas de union al receptor de glucagon y metodos para usarlas
US10961315B2 (en) 2018-07-27 2021-03-30 Ngm Biopharmaceuticals, Inc. Method of treating type I diabetes by administering a combination of a glucagon receptor antagonist and an anti-CD3 antibody

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4123427A (en) * 1976-07-27 1978-10-31 Daniel Thomas M Method for the purification of mycobacterial protein antigens and resulting product
GB2093039B (en) * 1981-01-29 1984-06-06 Nat Res Dev Improvements relating to hepatitis b vaccine
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
SE451843B (sv) * 1983-05-09 1987-11-02 Gambro Lundia Ab Forfarande for utvinning av en forening
EP0162078B1 (de) * 1983-10-28 1994-09-14 New England Medical Center Hospitals, Inc. VERFAHREN ZUR ISOLIERUNG EINES PROTEINS AUS EINER DIESES PROTEIN ENTHALTENDEN MISCHUNG UNTER VERWENDUNG EINES KONFORMATIONSSPEZIFISCHEN ANTIKöRPERS
US4568488A (en) * 1984-01-11 1986-02-04 Lee Huang Sylvia Reverse immunoaffinity chromatography purification method
US4499014A (en) * 1984-02-07 1985-02-12 Interferon Sciences, Inc. Method for purifying gamma-interferon
WO1986000910A1 (en) * 1984-07-30 1986-02-13 Celltech Limited Immunopurification process
US4762706A (en) * 1984-10-17 1988-08-09 Cetus Corporation Peptide antibodies and their use in detecting oncogene products
GB8501473D0 (en) * 1985-01-21 1985-02-20 Pasteur Institut Cloned dna sequences
DE3511011A1 (de) * 1985-03-27 1986-10-02 Hoechst Ag, 6230 Frankfurt Verfahren zur isolierung und reinigung von (alpha)-interferonen
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
GB8510177D0 (en) * 1985-04-22 1985-05-30 Public Health Laborotory Servi Isolating pituitary glycoprotein hormones
US4873312A (en) * 1985-04-25 1989-10-10 Amgen Method for purifying interferon and composition of matter produced thereby
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
IL81650A0 (en) * 1986-02-24 1987-09-16 Robapharm Ag Purified human angiogenic factor,method for its preparation and pharmaceutical preparations
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
JPS62298536A (ja) * 1986-06-17 1987-12-25 Green Cross Corp:The 生理活性物質の自動精製方法および自動精製装置
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
DE3855986T2 (de) * 1987-05-11 1998-02-05 Chiron Corp Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
US5136027A (en) * 1989-05-02 1992-08-04 Abbott Laboratories Method for renaturing proteins in the presence of alkyl sulfate detergents

Also Published As

Publication number Publication date
JPH05503081A (ja) 1993-05-27
DE69028711D1 (de) 1996-10-31
US5317092A (en) 1994-05-31
AU6750990A (en) 1991-06-13
DE69028711T2 (de) 1997-02-20
WO1991007428A1 (en) 1991-05-30
EP0502050A1 (de) 1992-09-09
DK580789D0 (da) 1989-11-20
EP0502050B1 (de) 1996-09-25

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Legal Events

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RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties