ATE146529T1 - Hybridisierungsverfahren und reagenzsatz dafür - Google Patents
Hybridisierungsverfahren und reagenzsatz dafürInfo
- Publication number
- ATE146529T1 ATE146529T1 AT88309414T AT88309414T ATE146529T1 AT E146529 T1 ATE146529 T1 AT E146529T1 AT 88309414 T AT88309414 T AT 88309414T AT 88309414 T AT88309414 T AT 88309414T AT E146529 T1 ATE146529 T1 AT E146529T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- copy
- hybridized
- target nucleic
- molecule
- Prior art date
Links
- 238000009396 hybridization Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 238000001914 filtration Methods 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 239000002853 nucleic acid probe Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI874466A FI80476C (fi) | 1987-10-09 | 1987-10-09 | Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE146529T1 true ATE146529T1 (de) | 1997-01-15 |
Family
ID=8525215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT88309414T ATE146529T1 (de) | 1987-10-09 | 1988-10-07 | Hybridisierungsverfahren und reagenzsatz dafür |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0317074B1 (de) |
| JP (1) | JP2705811B2 (de) |
| AT (1) | ATE146529T1 (de) |
| AU (1) | AU618278B2 (de) |
| DE (1) | DE3855709T2 (de) |
| DK (1) | DK174957B1 (de) |
| ES (1) | ES2095206T3 (de) |
| FI (1) | FI80476C (de) |
| GR (1) | GR3022457T3 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5753433A (en) * | 1909-12-05 | 1998-05-19 | Boehringer Mannheim Gmbh | Method for the sensitive detection of nucleic acids |
| US5856092A (en) * | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
| CA2011818A1 (en) * | 1989-03-17 | 1990-09-17 | Fred T. Oakes | Methods for purification, amplification and detection of a nucleic acid |
| FR2648152A1 (fr) * | 1989-06-12 | 1990-12-14 | Oris Ind Cie | Procede de detection et/ou d'identification d'acides nucleiques par amplification et hybridation en solution et ses applications |
| JPH03123500A (ja) * | 1989-10-06 | 1991-05-27 | Canon Inc | 核酸の分別方法 |
| DE4001154A1 (de) * | 1990-01-17 | 1991-07-18 | Boehringer Mannheim Gmbh | Verfahren zur herstellung von modifizierten nukleinsaeuren |
| CA2032331A1 (en) * | 1990-01-22 | 1991-07-23 | Annie L. Wu | Method and kits for detecting human leukocyte antigen dna |
| IL97222A (en) * | 1990-02-16 | 1995-08-31 | Orion Yhtymae Oy | Method and reagent for determining specific nucleotide variations |
| US6013431A (en) | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
| FR2660925B1 (fr) * | 1990-04-11 | 1994-07-01 | Inst Nat Sante Rech Med | Procede de fixation d'une sequence nucleotidique sur un support solide, applications et necessaires pour leur mise en óoeuvre. |
| US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
| US6071699A (en) | 1996-06-07 | 2000-06-06 | California Institute Of Technology | Nucleic acid mediated electron transfer |
| DK0840804T3 (da) * | 1996-01-23 | 2000-07-17 | Rapigene Inc | Fremgangsmåder og sammensætninger til analyse af nukleinsyremolekyler ved anvendelse af størrelsesseparationsteknikker |
| DE69700902T2 (de) * | 1996-01-23 | 2000-07-20 | Rapigene, Inc. | Verfahren zur erkennung von ligandenpaar bindung mit erhöter empfindlichkeit |
| WO1997035033A1 (en) | 1996-03-19 | 1997-09-25 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
| US6096273A (en) | 1996-11-05 | 2000-08-01 | Clinical Micro Sensors | Electrodes linked via conductive oligomers to nucleic acids |
| US7381525B1 (en) | 1997-03-07 | 2008-06-03 | Clinical Micro Sensors, Inc. | AC/DC voltage apparatus for detection of nucleic acids |
| US7070921B2 (en) | 2000-04-28 | 2006-07-04 | Molecular Devices Corporation | Molecular modification assays |
| US7745142B2 (en) | 1997-09-15 | 2010-06-29 | Molecular Devices Corporation | Molecular modification assays |
| US7632651B2 (en) | 1997-09-15 | 2009-12-15 | Mds Analytical Technologies (Us) Inc. | Molecular modification assays |
| US6982431B2 (en) | 1998-08-31 | 2006-01-03 | Molecular Devices Corporation | Sample analysis systems |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| WO2000063437A2 (en) | 1999-04-20 | 2000-10-26 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| AU779220B2 (en) | 1999-07-26 | 2005-01-13 | Clinical Micro Sensors, Inc. | Sequence determination of nucleic acids using electronic detection |
| US6518024B2 (en) | 1999-12-13 | 2003-02-11 | Motorola, Inc. | Electrochemical detection of single base extension |
| ATE412774T1 (de) | 2000-02-16 | 2008-11-15 | Illumina Inc | Parallele genotypisierung mehrerer patientenproben |
| EP1359228B1 (de) * | 2002-04-23 | 2013-11-27 | Accenture Global Services Limited | Nukleinsäure authentifikation mittels detektion des streulichtes |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO843838L (no) * | 1983-09-26 | 1985-03-27 | Ortho Diagnostic Systems Inc | Fremgangsmaate for detektering av nukleinsyre |
| EP0200113A3 (de) * | 1985-04-30 | 1987-03-18 | Pandex Laboratories, Inc. | Verfahren der Nukleinsäuren-Hybridisierungsprobe unter Zusatz eines lumineszierenden Labels |
| TW203120B (de) * | 1985-10-04 | 1993-04-01 | Abbott Lab | |
| JPS6298257A (ja) * | 1985-10-25 | 1987-05-07 | Nitto Electric Ind Co Ltd | 免疫学的測定法 |
| US4855225A (en) * | 1986-02-07 | 1989-08-08 | Applied Biosystems, Inc. | Method of detecting electrophoretically separated oligonucleotides |
| CA1290664C (en) * | 1986-03-05 | 1991-10-15 | Nanibhushan Dattagupta | Solution-phase single hybridization assay for detecting polynucleotide sequences |
| JPS62273453A (ja) * | 1986-05-21 | 1987-11-27 | Tosoh Corp | 免疫反応測定装置に用いるｂ／ｆ分離装置 |
| GB8629740D0 (en) * | 1986-12-12 | 1987-01-21 | Iq Bio Ltd | Immunoassay |
| AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
-
1987
- 1987-10-09 FI FI874466A patent/FI80476C/fi not_active IP Right Cessation
-
1988
- 1988-09-01 AU AU21781/88A patent/AU618278B2/en not_active Expired
- 1988-10-03 DK DK198805510A patent/DK174957B1/da not_active IP Right Cessation
- 1988-10-07 ES ES88309414T patent/ES2095206T3/es not_active Expired - Lifetime
- 1988-10-07 DE DE3855709T patent/DE3855709T2/de not_active Expired - Fee Related
- 1988-10-07 EP EP88309414A patent/EP0317074B1/de not_active Expired - Lifetime
- 1988-10-07 AT AT88309414T patent/ATE146529T1/de not_active IP Right Cessation
- 1988-10-08 JP JP63254666A patent/JP2705811B2/ja not_active Expired - Fee Related
-
1997
- 1997-01-31 GR GR970400168T patent/GR3022457T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU618278B2 (en) | 1991-12-19 |
| DE3855709T2 (de) | 1997-04-17 |
| DE3855709D1 (de) | 1997-01-30 |
| ES2095206T3 (es) | 1997-02-16 |
| JP2705811B2 (ja) | 1998-01-28 |
| AU2178188A (en) | 1989-04-13 |
| EP0317074B1 (de) | 1996-12-18 |
| EP0317074A2 (de) | 1989-05-24 |
| FI874466A0 (fi) | 1987-10-09 |
| GR3022457T3 (en) | 1997-04-30 |
| DK174957B1 (da) | 2004-03-22 |
| JPH01222800A (ja) | 1989-09-06 |
| FI80476B (fi) | 1990-02-28 |
| FI80476C (fi) | 1990-06-11 |
| DK551088D0 (da) | 1988-10-03 |
| FI874466L (fi) | 1989-04-10 |
| DK551088A (da) | 1989-04-10 |
| EP0317074A3 (de) | 1991-01-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EEIH | Change in the person of patent owner | ||
| REN | Ceased due to non-payment of the annual fee |