ES2095206T3 - Metodo de hibridacion y estuche de reactivos para el mismo. - Google Patents

Metodo de hibridacion y estuche de reactivos para el mismo.

Info

Publication number
ES2095206T3
ES2095206T3 ES88309414T ES88309414T ES2095206T3 ES 2095206 T3 ES2095206 T3 ES 2095206T3 ES 88309414 T ES88309414 T ES 88309414T ES 88309414 T ES88309414 T ES 88309414T ES 2095206 T3 ES2095206 T3 ES 2095206T3
Authority
ES
Spain
Prior art keywords
nucleic acid
molecula
same
hybridization method
diane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88309414T
Other languages
English (en)
Inventor
Hans Erik Soderlund
Tuula Marjut Ranki
Ann-Christine Elizabet Syvanen
Petri Uolevi Buchert
Anne Maritta Jarvinen
Stefan Henrik Karlsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sangtec Medical AB
Original Assignee
Sangtec Medical AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sangtec Medical AB filed Critical Sangtec Medical AB
Application granted granted Critical
Publication of ES2095206T3 publication Critical patent/ES2095206T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

UN METODO DE HIBRIDACION DE DETECCION DE ACIDO NUCLEICO QUE COMPRENDE: (A) FILTRACION DE UNA DISOLUCION EN LA QUE LA MOLECULA PRESENCIA DEL ACIDO NUCLEICO DIANA ES DETECTADO PARA SEPARAR IMPUREZAS INSOLUBLES DE LA SOLUCION (B) PRODUCCION A PARTIR DEL FILTRADO DE UN CONFIGADO DE UNA PARTICULA INSOLUBLE Y ACIDO NUCLEICO DIANA HIBRIDIZADO O COPIA HIBRIDIZADA DE MOLECULA DE ACIDO NUCLEICO DIANA, UNIENDO EL ACIDO A LA PARTICULA A TRAVES DE UNA SONDA DE ACIDO NUCLEICO O OPCIONALMENTE, EN EL CASO DE MOLECULA COPIA HIBRIDIZADA A TRAVES DE UN PRIMERO POR MEDIO DEL CUAL SE HACE LA COPIA; (C) SEPARACION DEL CONJUGADO DE CUALQUIER SONDA EN EXCESO Y/O PRIMERO POR FILTRACION Y LAVADO; Y (D) DETECCION DE LA CANTIDAD DE CONJUGADO PRESENTE.
ES88309414T 1987-10-09 1988-10-07 Metodo de hibridacion y estuche de reactivos para el mismo. Expired - Lifetime ES2095206T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI874466A FI80476C (fi) 1987-10-09 1987-10-09 Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning.

Publications (1)

Publication Number Publication Date
ES2095206T3 true ES2095206T3 (es) 1997-02-16

Family

ID=8525215

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88309414T Expired - Lifetime ES2095206T3 (es) 1987-10-09 1988-10-07 Metodo de hibridacion y estuche de reactivos para el mismo.

Country Status (9)

Country Link
EP (1) EP0317074B1 (es)
JP (1) JP2705811B2 (es)
AT (1) ATE146529T1 (es)
AU (1) AU618278B2 (es)
DE (1) DE3855709T2 (es)
DK (1) DK174957B1 (es)
ES (1) ES2095206T3 (es)
FI (1) FI80476C (es)
GR (1) GR3022457T3 (es)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5753433A (en) * 1909-12-05 1998-05-19 Boehringer Mannheim Gmbh Method for the sensitive detection of nucleic acids
US5856092A (en) * 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
CA2011818A1 (en) * 1989-03-17 1990-09-17 Fred T. Oakes Methods for purification, amplification and detection of a nucleic acid
FR2648152A1 (fr) * 1989-06-12 1990-12-14 Oris Ind Cie Procede de detection et/ou d'identification d'acides nucleiques par amplification et hybridation en solution et ses applications
JPH03123500A (ja) * 1989-10-06 1991-05-27 Canon Inc 核酸の分別方法
DE4001154A1 (de) * 1990-01-17 1991-07-18 Boehringer Mannheim Gmbh Verfahren zur herstellung von modifizierten nukleinsaeuren
CA2032331A1 (en) * 1990-01-22 1991-07-23 Annie L. Wu Method and kits for detecting human leukocyte antigen dna
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
IL97222A (en) * 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and reagent for determining specific nucleotide variations
FR2660925B1 (fr) * 1990-04-11 1994-07-01 Inst Nat Sante Rech Med Procede de fixation d'une sequence nucleotidique sur un support solide, applications et necessaires pour leur mise en óoeuvre.
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
CN1202260C (zh) * 1996-01-23 2005-05-18 佳根基因组学公司 利用大小分离技术分析核酸分子的方法和组合物
HUP9900459A3 (en) * 1996-01-23 1999-11-29 Rapigene Inc Bothell Methods and compositions for detecting binding of ligand pair using non-fluorescent label
WO1997035033A1 (en) 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US7381525B1 (en) 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
EP1923471B1 (en) 1999-04-20 2012-12-19 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
WO2001007665A2 (en) 1999-07-26 2001-02-01 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
US6518024B2 (en) 1999-12-13 2003-02-11 Motorola, Inc. Electrochemical detection of single base extension
WO2001061043A2 (en) 2000-02-16 2001-08-23 Illumina, Inc. Parallel genotyping of multiple patient samples
EP1359228B1 (en) * 2002-04-23 2013-11-27 Accenture Global Services Limited DNA authentification based on scattered-light detection

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO843838L (no) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc Fremgangsmaate for detektering av nukleinsyre
EP0200113A3 (en) * 1985-04-30 1987-03-18 Pandex Laboratories, Inc. A method of solid phase nucleic acid hybridization assay incorporating a luminescent label
TW203120B (es) * 1985-10-04 1993-04-01 Abbott Lab
JPS6298257A (ja) * 1985-10-25 1987-05-07 Nitto Electric Ind Co Ltd 免疫学的測定法
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
CA1290664C (en) * 1986-03-05 1991-10-15 Nanibhushan Dattagupta Solution-phase single hybridization assay for detecting polynucleotide sequences
JPS62273453A (ja) * 1986-05-21 1987-11-27 Tosoh Corp 免疫反応測定装置に用いるｂ／ｆ分離装置
GB8629740D0 (en) * 1986-12-12 1987-01-21 Iq Bio Ltd Immunoassay
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore

Also Published As

Publication number Publication date
GR3022457T3 (en) 1997-04-30
FI80476B (fi) 1990-02-28
AU618278B2 (en) 1991-12-19
DK551088A (da) 1989-04-10
JPH01222800A (ja) 1989-09-06
FI874466A0 (fi) 1987-10-09
DK551088D0 (da) 1988-10-03
ATE146529T1 (de) 1997-01-15
DK174957B1 (da) 2004-03-22
EP0317074B1 (en) 1996-12-18
DE3855709T2 (de) 1997-04-17
JP2705811B2 (ja) 1998-01-28
EP0317074A2 (en) 1989-05-24
EP0317074A3 (en) 1991-01-30
FI80476C (fi) 1990-06-11
FI874466L (fi) 1989-04-10
DE3855709D1 (de) 1997-01-30
AU2178188A (en) 1989-04-13

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