ATE147104T1 - Gekoppelte transkription und translation in zellfreien eukaryoten-extrakten - Google Patents

Gekoppelte transkription und translation in zellfreien eukaryoten-extrakten

Info

Publication number
ATE147104T1
ATE147104T1 AT92922343T AT92922343T ATE147104T1 AT E147104 T1 ATE147104 T1 AT E147104T1 AT 92922343 T AT92922343 T AT 92922343T AT 92922343 T AT92922343 T AT 92922343T AT E147104 T1 ATE147104 T1 AT E147104T1
Authority
AT
Austria
Prior art keywords
translation
free
cell
eukaryote
extracts
Prior art date
Application number
AT92922343T
Other languages
English (en)
Inventor
David V Thompson
Oosbree Thomas R Van
Gregory S Beckler
Herwynen John F Van
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25103433&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE147104(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE147104T1 publication Critical patent/ATE147104T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
AT92922343T 1991-10-11 1992-10-07 Gekoppelte transkription und translation in zellfreien eukaryoten-extrakten ATE147104T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US77513691A 1991-10-11 1991-10-11

Publications (1)

Publication Number Publication Date
ATE147104T1 true ATE147104T1 (de) 1997-01-15

Family

ID=25103433

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92922343T ATE147104T1 (de) 1991-10-11 1992-10-07 Gekoppelte transkription und translation in zellfreien eukaryoten-extrakten

Country Status (10)

Country Link
US (1) US5324637A (de)
EP (1) EP0566714B1 (de)
JP (1) JP2904583B2 (de)
AT (1) ATE147104T1 (de)
AU (1) AU660329B2 (de)
DE (1) DE69216385T2 (de)
DK (1) DK0566714T3 (de)
ES (1) ES2097363T3 (de)
GR (1) GR3022719T3 (de)
WO (1) WO1993007287A1 (de)

Families Citing this family (49)

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US5492817A (en) * 1993-11-09 1996-02-20 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
US5665563A (en) * 1991-10-11 1997-09-09 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
AU4843893A (en) * 1992-09-02 1994-03-29 Scripps Research Institute, The Coupled isothermal polynucleotide amplification and translation system
WO1994006928A1 (en) * 1992-09-24 1994-03-31 The Public Health Research Institute Of The City Of New York, Inc. Coupled replication-translation methods and kits for protein synthesis
KR0131166B1 (ko) * 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법
US5874231A (en) * 1994-08-22 1999-02-23 Mcgill University Methods of screening for non-hormone compounds which effect modulation of polypeptide translation
WO1996014428A1 (en) * 1994-11-08 1996-05-17 Novagen, Inc. Method for in vitro protein synthesis
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
EP0954585B1 (de) 1996-10-01 2009-11-25 Geron Corporation Menschlicher telomerase reverse transcriptase promoter
US7638269B2 (en) * 1997-02-07 2009-12-29 The Regents Of The University Of California Viral capsid assembly intermediates and methods of production
WO1998035062A1 (en) * 1997-02-07 1998-08-13 Lingappa Jaisri R Multistep, atp-dependent cell-free system for the assembly of human immunodeficiency virus capsids
US6207370B1 (en) 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6994986B2 (en) 1999-03-17 2006-02-07 The Board Of Trustees Of The Leland Stanford University In vitro synthesis of polypeptides by optimizing amino acid metabolism
US6168931B1 (en) 1999-03-17 2001-01-02 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of biological macromolecules using a novel ATP regeneration system
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
EA006162B1 (ru) * 1999-05-11 2005-10-27 Селлфри Сайенсиз Ко., Лтд. Препарат для бесклеточного синтеза белка, способ синтеза белка с использованием данного препарата и устройство для его осуществления
US20030091999A1 (en) * 1999-10-01 2003-05-15 Zhongping Yu Compositions and methods for identifying polypeptides and nucleic acid molecules
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
EP1278883B1 (de) * 2000-05-03 2004-12-29 EMD Biosciences, Inc. E. coli extrakt zur synthese von proteinen
US6878861B2 (en) * 2000-07-21 2005-04-12 Washington State University Research Foundation Acyl coenzyme A thioesterases
KR100399337B1 (ko) * 2001-02-07 2003-09-26 드림바이오젠 주식회사 단백질의 무세포 번역후수식법
JP4328530B2 (ja) * 2001-03-08 2009-09-09 ライフ テクノロジーズ コーポレーション 改良インビトロ合成系
US7063946B2 (en) 2001-09-10 2006-06-20 Meso Scale Technologies, Llc. Methods, reagents, kits and apparatus for protein function analysis
US8034581B2 (en) * 2001-11-26 2011-10-11 Toshio Hara Cell-free extract and glycoprotein synthesis system
DE60316541D1 (de) * 2002-01-31 2007-11-08 Cellfree Sciences Co Ltd Keimextrakt zur zellfreien proteinsynthese und verfahren zu dessen herstellung
US7744816B2 (en) 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
US8278055B2 (en) * 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
CN1738914A (zh) 2002-11-21 2006-02-22 震源技术公司 使用编码双链启动子一条链的引物的方法
US20060141054A1 (en) 2004-10-25 2006-06-29 Thomas Piccariello Metal coordinated compositions
US8779175B2 (en) 2004-10-25 2014-07-15 Synthonics, Inc. Coordination complexes, pharmaceutical solutions comprising coordination complexes, and methods of treating patients
US7799937B2 (en) * 2004-10-25 2010-09-21 Synthonics, Inc. Metal coordinated compositions
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
EP2118129A4 (de) * 2007-03-01 2010-04-28 Life Technologies Corp Isolierte phospholipidproteinpartikel
US8470966B2 (en) 2007-08-10 2013-06-25 Protelica, Inc. Universal fibronectin type III binding-domain libraries
EP2222846B1 (de) * 2007-08-10 2017-05-03 Protelix, Inc. Universelle fibronectin-typ iii-bindungsdomäne-bibliotheken
US8680019B2 (en) * 2007-08-10 2014-03-25 Protelica, Inc. Universal fibronectin Type III binding-domain libraries
EP2193146B1 (de) 2007-09-14 2016-05-25 Adimab, LLC Synthetische antikörperbibliotheken mit rationalem design und ihre verwendung
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
WO2011051333A1 (en) 2009-10-30 2011-05-05 Novartis Ag Universal fibronectin type iii bottom-side binding domain libraries
WO2011131231A1 (en) 2010-04-20 2011-10-27 INSTITUT FüR MIKROTECHNIK MAINZ GMBH System for the in vitro transcription and translation of membrane proteins
EP3741883B1 (de) 2010-07-16 2022-12-14 Adimab, LLC Antikörperbibliotheken
RU2013108962A (ru) 2010-07-30 2014-09-10 Новартис Аг Молекулы фибронектина, содержащие карман, и их библиотеки
WO2015089080A2 (en) 2013-12-09 2015-06-18 Adimab, Llc Polyclonal mixtures of antibodies, and methods of making and using them
US10787500B2 (en) 2015-04-10 2020-09-29 Adimab, Llc Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species
JP7636135B2 (ja) * 2017-02-09 2025-02-26 コルテバ アグリサイエンス エルエルシー 人工エネルギー再生システムを必要としない新規の無真核細胞発現システム
CN113409887A (zh) * 2021-07-07 2021-09-17 中国科学院生物物理研究所 一种无细胞翻译体系、方法及产物

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1705302A1 (ru) * 1988-12-22 1992-01-15 Институт Белка Ан Ссср Способ препаративной экспрессии генов в бесклеточной системе сопр женной транскрипции/трансл ции
ATE147787T1 (de) * 1989-07-31 1997-02-15 Inst Of Protein Research Russi Verfahren zur herstellung von polypeptiden in einem zellfreien system

Also Published As

Publication number Publication date
EP0566714A4 (en) 1994-07-13
US5324637A (en) 1994-06-28
DE69216385D1 (de) 1997-02-13
GR3022719T3 (en) 1997-06-30
ES2097363T3 (es) 1997-04-01
WO1993007287A1 (en) 1993-04-15
EP0566714A1 (de) 1993-10-27
EP0566714B1 (de) 1997-01-02
JP2904583B2 (ja) 1999-06-14
JPH06503477A (ja) 1994-04-21
DE69216385T2 (de) 1997-06-12
AU2792192A (en) 1993-05-03
DK0566714T3 (da) 1997-06-16
AU660329B2 (en) 1995-06-22

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