ES2097363T3 - Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas. - Google Patents

Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas.

Info

Publication number
ES2097363T3
ES2097363T3 ES92922343T ES92922343T ES2097363T3 ES 2097363 T3 ES2097363 T3 ES 2097363T3 ES 92922343 T ES92922343 T ES 92922343T ES 92922343 T ES92922343 T ES 92922343T ES 2097363 T3 ES2097363 T3 ES 2097363T3
Authority
ES
Spain
Prior art keywords
transcription
euchariot
excerpt
exempt
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92922343T
Other languages
English (en)
Inventor
David V Thompson
Oosbree Thomas R Van
Gregory S Beckler
Herwynen John F Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25103433&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2097363(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ES2097363T3 publication Critical patent/ES2097363T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

UN METODO PARA LIGAR LA TRANSCRIPCION Y TRANSLACION DE UN ADN UTILIZANDO UNA SOLUCION QUE COMPRENDE UN EXTRACTO LIBRE DE CELULAS CONSISTE EN LA ADICION DE UNA CANTIDAD SUFICIENTE DE UN COMPUESTO DE MAGNESIO AL EXTRACTO PARA AUMENTAR LA CONCENTRACION DE MAGNESIO A UN NIVEL DONDE EL ARN SE TANSCRIBE DESDE EL ADN Y SE TRASLADA A UNA PROTEINA.
ES92922343T 1991-10-11 1992-10-07 Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas. Expired - Lifetime ES2097363T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US77513691A 1991-10-11 1991-10-11

Publications (1)

Publication Number Publication Date
ES2097363T3 true ES2097363T3 (es) 1997-04-01

Family

ID=25103433

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92922343T Expired - Lifetime ES2097363T3 (es) 1991-10-11 1992-10-07 Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas.

Country Status (10)

Country Link
US (1) US5324637A (es)
EP (1) EP0566714B1 (es)
JP (1) JP2904583B2 (es)
AT (1) ATE147104T1 (es)
AU (1) AU660329B2 (es)
DE (1) DE69216385T2 (es)
DK (1) DK0566714T3 (es)
ES (1) ES2097363T3 (es)
GR (1) GR3022719T3 (es)
WO (1) WO1993007287A1 (es)

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US5492817A (en) * 1993-11-09 1996-02-20 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
US5665563A (en) * 1991-10-11 1997-09-09 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
WO1994005812A1 (en) * 1992-09-02 1994-03-17 The Scripps Research Institute Coupled isothermal polynucleotide amplification and translation system
AU4930993A (en) * 1992-09-24 1994-04-12 Public Health Research Institute Of The City Of New York, Inc., The Coupled replication-translation methods and kits for protein synthesis
KR0131166B1 (ko) * 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법
US5874231A (en) * 1994-08-22 1999-02-23 Mcgill University Methods of screening for non-hormone compounds which effect modulation of polypeptide translation
WO1996014428A1 (en) * 1994-11-08 1996-05-17 Novagen, Inc. Method for in vitro protein synthesis
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
EA199900355A1 (ru) 1996-10-01 2000-08-28 Джерон Корпорейшн Теломераза обратная тринскриптаза
US6593103B1 (en) * 1997-02-07 2003-07-15 The Regents Of The University Of California HIV capsid assembly-associated compositions and method
US7638269B2 (en) * 1997-02-07 2009-12-29 The Regents Of The University Of California Viral capsid assembly intermediates and methods of production
US6207370B1 (en) 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6168931B1 (en) 1999-03-17 2001-01-02 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of biological macromolecules using a novel ATP regeneration system
US6994986B2 (en) 1999-03-17 2006-02-07 The Board Of Trustees Of The Leland Stanford University In vitro synthesis of polypeptides by optimizing amino acid metabolism
RU2169154C2 (ru) * 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
DE69940525D1 (de) * 1999-05-11 2009-04-16 Cellfree Sciences Co Ltd Zubereitung, die einen zellextrakt zur herstellung eines zellfreien proteins enthalten und vorrichtung zur herstellung eines zellfreien proteins
US20030091999A1 (en) * 1999-10-01 2003-05-15 Zhongping Yu Compositions and methods for identifying polypeptides and nucleic acid molecules
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US7008651B2 (en) * 2000-05-03 2006-03-07 Emd Biosciences, Inc. E. coli extract for protein synthesis
US6878861B2 (en) * 2000-07-21 2005-04-12 Washington State University Research Foundation Acyl coenzyme A thioesterases
KR100399337B1 (ko) * 2001-02-07 2003-09-26 드림바이오젠 주식회사 단백질의 무세포 번역후수식법
CA2440531A1 (en) * 2001-03-08 2002-09-19 Invitrogen Corporation Improved in vitro synthesis system
EP1451348B1 (en) 2001-09-10 2015-08-12 Meso Scale Technologies, LLC Methods, reagents, kits and apparatus for protein function analysis
US8034581B2 (en) * 2001-11-26 2011-10-11 Toshio Hara Cell-free extract and glycoprotein synthesis system
CA2474466A1 (en) * 2002-01-31 2003-08-07 Yaeta Endo Germ extract for cell-free protein synthesis and process for producing the same
US8278055B2 (en) * 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US7744816B2 (en) 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
AU2003297557B2 (en) 2002-11-21 2009-02-26 Cellscript, Inc. Methods for using primers that encode one strand of a double-stranded promoter
US7799937B2 (en) * 2004-10-25 2010-09-21 Synthonics, Inc. Metal coordinated compositions
US20060141054A1 (en) * 2004-10-25 2006-06-29 Thomas Piccariello Metal coordinated compositions
US8779175B2 (en) * 2004-10-25 2014-07-15 Synthonics, Inc. Coordination complexes, pharmaceutical solutions comprising coordination complexes, and methods of treating patients
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
WO2008106660A2 (en) * 2007-03-01 2008-09-04 Invitrogen Corporation Isolated phospholipid-protein particles
US20090176654A1 (en) * 2007-08-10 2009-07-09 Protelix, Inc. Universal fibronectin type III binding-domain libraries
US8470966B2 (en) 2007-08-10 2013-06-25 Protelica, Inc. Universal fibronectin type III binding-domain libraries
US8680019B2 (en) * 2007-08-10 2014-03-25 Protelica, Inc. Universal fibronectin Type III binding-domain libraries
WO2009036379A2 (en) 2007-09-14 2009-03-19 Adimab, Inc. Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US9139825B2 (en) 2009-10-30 2015-09-22 Novartis Ag Universal fibronectin type III bottom-side binding domain libraries
US20130203110A1 (en) 2010-04-20 2013-08-08 Institut Fuer Mikrotechnik Mainz Gmbh System for the in vitro transcription and translation of membrane proteins
MX381912B (es) 2010-07-16 2025-03-13 Adimab Llc Colecciones de anticuerpos.
AU2011283646B2 (en) 2010-07-30 2015-07-09 Novartis Ag Fibronectin cradle molecules and libraries thereof
WO2015089080A2 (en) 2013-12-09 2015-06-18 Adimab, Llc Polyclonal mixtures of antibodies, and methods of making and using them
EP3280444B1 (en) 2015-04-10 2024-05-08 Adimab, LLC Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species
CA3052966A1 (en) * 2017-02-09 2018-08-16 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. Novel eukaryotic cell-free expression system that does not require an artificial energy regeneration system
CN113409887A (zh) * 2021-07-07 2021-09-17 中国科学院生物物理研究所 一种无细胞翻译体系、方法及产物

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1705302A1 (ru) * 1988-12-22 1992-01-15 Институт Белка Ан Ссср Способ препаративной экспрессии генов в бесклеточной системе сопр женной транскрипции/трансл ции
WO1991002076A1 (en) * 1989-07-31 1991-02-21 Institut Belka Akademii Nauk Sssr Method for obtaining polypeptides in a cell-free system

Also Published As

Publication number Publication date
JP2904583B2 (ja) 1999-06-14
EP0566714A4 (en) 1994-07-13
DE69216385T2 (de) 1997-06-12
DE69216385D1 (de) 1997-02-13
GR3022719T3 (en) 1997-06-30
WO1993007287A1 (en) 1993-04-15
EP0566714B1 (en) 1997-01-02
DK0566714T3 (da) 1997-06-16
EP0566714A1 (en) 1993-10-27
US5324637A (en) 1994-06-28
ATE147104T1 (de) 1997-01-15
AU2792192A (en) 1993-05-03
AU660329B2 (en) 1995-06-22
JPH06503477A (ja) 1994-04-21

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