ATE148230T1 - Kinetische bestimmungsmethode für endotoxin unter verwendung von limulus-amoebocyten-lysat und chromogenem substrat - Google Patents

Kinetische bestimmungsmethode für endotoxin unter verwendung von limulus-amoebocyten-lysat und chromogenem substrat

Info

Publication number
ATE148230T1
ATE148230T1 AT90311771T AT90311771T ATE148230T1 AT E148230 T1 ATE148230 T1 AT E148230T1 AT 90311771 T AT90311771 T AT 90311771T AT 90311771 T AT90311771 T AT 90311771T AT E148230 T1 ATE148230 T1 AT E148230T1
Authority
AT
Austria
Prior art keywords
endotoxin
chromogenic substrate
determination method
amoebocyte lysate
limulus amoebocyte
Prior art date
Application number
AT90311771T
Other languages
English (en)
Inventor
Ronald N Berzofsky
Original Assignee
Biowhittaker Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23698313&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE148230(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Biowhittaker Inc filed Critical Biowhittaker Inc
Application granted granted Critical
Publication of ATE148230T1 publication Critical patent/ATE148230T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT90311771T 1989-10-30 1990-10-26 Kinetische bestimmungsmethode für endotoxin unter verwendung von limulus-amoebocyten-lysat und chromogenem substrat ATE148230T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US42830089A 1989-10-30 1989-10-30

Publications (1)

Publication Number Publication Date
ATE148230T1 true ATE148230T1 (de) 1997-02-15

Family

ID=23698313

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90311771T ATE148230T1 (de) 1989-10-30 1990-10-26 Kinetische bestimmungsmethode für endotoxin unter verwendung von limulus-amoebocyten-lysat und chromogenem substrat

Country Status (10)

Country Link
US (1) US5310657A (de)
EP (1) EP0426395B1 (de)
JP (1) JP2955345B2 (de)
AT (1) ATE148230T1 (de)
AU (1) AU644109B2 (de)
CA (1) CA2028789C (de)
DE (1) DE69029785T2 (de)
DK (1) DK0426395T3 (de)
ES (1) ES2098256T3 (de)
GR (1) GR3023298T3 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0549102B1 (de) * 1991-12-25 1996-07-03 Maruha Corporation Reagenzien und Verfahren zur Bestimmung von Beta-Glucanen
JP3242733B2 (ja) * 1993-02-26 2001-12-25 生化学工業株式会社 エンドトキシン特異的測定剤
US6270982B1 (en) 1997-10-09 2001-08-07 Charles River Laboratories Methods and compositions for the detection of bacterial endotoxins
JP4235279B2 (ja) 1998-04-10 2009-03-11 生化学工業株式会社 新規酵素反応測定法
EP1499643A4 (de) * 2002-04-30 2008-04-16 Biowhittaker Technologies Inc Automatisierte analysesysteme mit sequentieller injektion zur bestimmung von endotoxingehalten im spurenbereich
DE60326988D1 (de) * 2002-12-20 2009-05-14 Charlotte Mecklenburg Hospital Wegwerf-handvorrichtung zum auffangen von ausgeatmetem atemkondensat
US7828741B2 (en) * 2002-12-20 2010-11-09 The Charlotte-Mecklenburg Hospital Authority Utilizing lipopolysaccharide in exhaled breath condensate to diagnose gram negative pneumonia
WO2004073497A2 (en) * 2003-02-14 2004-09-02 The Charlotte-Mecklenburg Hospital Authority Device and method for collection of exhaled alveolar breath condensate
JP5118849B2 (ja) * 2003-03-17 2013-01-16 チャールズ リバー ラボラトリーズ， インコーポレイテッド 微生物夾雑物の検出のための方法および組成物
EP1627040A2 (de) * 2003-04-18 2006-02-22 Associates Of Cape Cod, Inc. Kit zum nachweis von endotoxin in wässrigen lösungen
US20050048655A1 (en) * 2003-04-18 2005-03-03 Novitsky Thomas J. Kit for detecting endotoxin
AU2004290384A1 (en) * 2003-11-07 2005-05-26 Cambrex Bio Science Walkersville, Inc. On-line apparatus and method for determining endotoxin levels
WO2007078268A2 (en) * 2004-12-02 2007-07-12 Charles River Laboratories, Inc. Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants
ATE419526T1 (de) 2005-01-13 2009-01-15 Charles River Lab Inc Verfahren zur klassifizierung von mikroben in einer biologischen probe
US20090208995A1 (en) * 2006-07-07 2009-08-20 Seikagaku Corporation Pro-clotting enzyme, and method for detection of endotoxin or (1-3)-beta-d-glucan using the same
US20080124248A1 (en) * 2006-11-29 2008-05-29 Edward Andrew Sobek Endotoxins and glucans quantification in indoor air
WO2009137244A1 (en) * 2008-04-15 2009-11-12 Charles River Laboratories, Inc. Cartridge and method for sample analysis
JP5489680B2 (ja) * 2009-12-02 2014-05-14 興和株式会社 生物由来の生理活性物質の測定方法、それを実行するためのプログラム及び、生物由来の生理活性物質の測定装置
US8507282B2 (en) 2009-03-13 2013-08-13 Kowa Company, Ltd. Method for measuring physiologically active substance of biological origin, program for implementing the same, and apparatus for measuring physiologically active substance of biological origin
EP2446018B1 (de) 2009-06-26 2017-01-18 Charles River Laboratories, Inc. Wärmebehandelte limulus-amebocyt-lysaten
GB2477752A (en) * 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
CU24073B1 (es) 2011-12-27 2015-01-29 Ct De Investigación Y Desarrollo De Medicamentos Cidem COMPOSICIÓN A PARTIR DE EXTRACTO DE HEMOCITOS DE LANGOSTA PARA LA DETECCIÓN DE LIPOPOLISACÁRIDOS, PEPTIDOGLICANOS Y 1,3-ß-D-GLUCANOS
US20130078665A1 (en) * 2012-07-18 2013-03-28 Deepika Bodapati Test strip, a test kit and a method for detection of endotoxin in food
US20150293097A1 (en) 2012-10-08 2015-10-15 General Electric Company Preloaded test substrates for testing lal-reactive substances, methods of use, and methods of making
US10309947B2 (en) 2013-02-27 2019-06-04 Trace-Ability, Inc. System and method for radiosynthesis, quality control and dose dispensing
US11002717B2 (en) 2014-02-26 2021-05-11 Trace-Ability, Inc. Systems and methods for characterizing radioactive analytes
US10895563B2 (en) 2014-02-26 2021-01-19 Trace-Ability, Inc. Palette-based systems for analyte characterization
JP7044222B2 (ja) * 2016-08-03 2022-03-30 ロンザ ウォーカーズヴィル，インコーポレーテッド 実質的にコアギュローゲンを含まないリムルスアメボサイトライセートを使用してエンドトキシンを検出する方法
WO2018132562A1 (en) 2017-01-11 2018-07-19 Lonza Walkersville, Inc. Coagulogen-free clarified limulus amebocyte lysate
KR20200019739A (ko) 2017-06-28 2020-02-24 론자 워커스빌 아이엔씨. 내독소 검출용 카트리지
US11892397B2 (en) * 2017-10-17 2024-02-06 Board Of Regents, The University Of Texas System Endotoxin testing assay and method of same
CN109387637A (zh) * 2018-10-12 2019-02-26 四川升和药业股份有限公司 丹参注射液细菌内毒素检测方法
CN111077307B (zh) * 2018-10-21 2022-08-23 厦门鲎试剂生物科技股份有限公司 一种用革兰氏阴性细菌感染快速检测脓毒病的新方法
US12158471B2 (en) 2022-02-11 2024-12-03 Charles River Laboratories, Inc. Recombinant amebocyte lysate and uses thereof
WO2024254215A1 (en) 2023-06-05 2024-12-12 Charles River Laboratories, Inc. Recombinant amebocyte clotting factors and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5415797A (en) * 1977-06-14 1979-02-05 Seikagaku Kogyo Co Ltd Detection and measurement of toxin in cells
US4322217A (en) * 1980-06-27 1982-03-30 Mallinckrodt, Inc. Process for preparing Limulus lysate
US4406832A (en) * 1981-09-03 1983-09-27 Mallinckrodt, Inc. Peptide-type substrates useful in the quantitative determination of endotoxin
US4510241A (en) * 1981-09-03 1985-04-09 Mallinckrodt, Inc. Peptide-type substrates useful in the quantitative determination of endotoxin
DE3439761A1 (de) * 1984-10-31 1986-05-07 Klaus-Peter 6800 Mannheim Becker Verfahren zur bestimmung von endotoxinkonzentrationen

Also Published As

Publication number Publication date
JP2955345B2 (ja) 1999-10-04
GR3023298T3 (en) 1997-07-30
DE69029785D1 (de) 1997-03-06
JPH03220456A (ja) 1991-09-27
US5310657A (en) 1994-05-10
ES2098256T3 (es) 1997-05-01
EP0426395B1 (de) 1997-01-22
AU644109B2 (en) 1993-12-02
EP0426395A1 (de) 1991-05-08
DE69029785T2 (de) 1997-05-28
AU6555690A (en) 1991-05-02
DK0426395T3 (de) 1997-03-03
CA2028789C (en) 2001-02-06
CA2028789A1 (en) 1991-05-01

Similar Documents

Publication Publication Date Title
ATE148230T1 (de) Kinetische bestimmungsmethode für endotoxin unter verwendung von limulus-amoebocyten-lysat und chromogenem substrat
ATE58600T1 (de) Testverfahren.
DE68928994D1 (de) Elektrochemilumineszente testverfahren
ES2054617T3 (es) Nuevo metodo para la deteccion y/o determinacion cuantitativa de agentes ligantes especificos y sus correspondientes sustancias ligables.
ES2148225T3 (es) Reactivos y procedimientos que permiten la deteccion y la cuantificacion de la tiroxina en muestras de fluido.
ATE201771T1 (de) Spezifische bindungstests und reagenzien dafür
FI915150A7 (fi) Menetelmät, reagenssit ja testikäyttöpakkaukset biologisten yksiköiden alapopulaatioiden määrittämiseksi
ATE128237T1 (de) Test für hepatitis-c.
DE59309900D1 (de) Verfahren zur Analyse partikelverstärkter Agglutinationsreaktionen auf Zentrifugalanalysatoren durch Bestimmung der Trübungsaufhellung
IL74303A (en) Compositions and test device for determining the presence of leukocytes,esterase and protease in biological samples
ES2098406T3 (es) Ensayo funcional y reactivo para la determinacion de fibrinogeno.
ATE193600T1 (de) Verfahren zur stabilisierung von enzym-konjugaten
ATE206746T1 (de) Reinigungslösung für analysegerät
SE9604378D0 (sv) Methods and reagents for determining protein S
DE3650691D1 (de) Verfahren zum Messen von Freitestosteronen in biologischen Flüssigkeiten
ATE67597T1 (de) Verfahren und materialien zur durchfuehrung von spezifischen bindungstests.
ES2076221T3 (es) Derivados geminales de difenilo y su uso en inmunoensayos.
DE69526056D1 (de) Immunologisches Testverfahren zur Quantifizierung von PEG-modifiziertem humanem Granulozyten-Kolonie-Stimulierender-Faktor
DE58907725D1 (de) Mittel für immunchemische Tests enthaltend Aminoxide.
ATE278193T1 (de) Verfahren zum testen eines asialoglycoproteinrezeptors und testreagenz dafür
ES2169211T3 (es) Ensayos de proteccion de micelas.
IT1237809B (it) Metodo per la determinazione qualitativa e quantitativa di anticorpi diretti contro antigeni batterici attraverso la misurazione fotometrica dell'agglutinazione.
IL160531A0 (en) Compositions and methods for aa4rp assay
ES2016992B3 (es) Reagente y procedimiento en la determinacion c 3a mediante ensayo inmunoquimico.
DK576789A (da) Fremgangsmaade til bestemmelse af tilstedevaerelsen af endotoksin i en proeve

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time