ATE105856T1 - Serumfreie wachstumsmedien für insektenzellen und expression von produkten damit. - Google Patents
Serumfreie wachstumsmedien für insektenzellen und expression von produkten damit.Info
- Publication number
- ATE105856T1 ATE105856T1 AT88906600T AT88906600T ATE105856T1 AT E105856 T1 ATE105856 T1 AT E105856T1 AT 88906600 T AT88906600 T AT 88906600T AT 88906600 T AT88906600 T AT 88906600T AT E105856 T1 ATE105856 T1 AT E105856T1
- Authority
- AT
- Austria
- Prior art keywords
- serum
- products
- insect cells
- expression
- growth media
- Prior art date
Links
- 241000238631 Hexapoda Species 0.000 title abstract 4
- 239000001963 growth medium Substances 0.000 title 1
- 230000010261 cell growth Effects 0.000 abstract 2
- 210000002966 serum Anatomy 0.000 abstract 2
- 239000004017 serum-free culture medium Substances 0.000 abstract 2
- 230000003612 virological effect Effects 0.000 abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 230000012010 growth Effects 0.000 abstract 1
- 239000012679 serum free medium Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0601—Invertebrate cells or tissues, e.g. insect cells; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/74—Undefined extracts from fungi, e.g. yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/80—Undefined extracts from animals
- C12N2500/84—Undefined extracts from animals from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/077,303 US5024947A (en) | 1987-07-24 | 1987-07-24 | Serum free media for the growth on insect cells and expression of products thereby |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE105856T1 true ATE105856T1 (de) | 1994-06-15 |
Family
ID=22137290
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT88906600T ATE105856T1 (de) | 1987-07-24 | 1988-07-20 | Serumfreie wachstumsmedien für insektenzellen und expression von produkten damit. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5024947A (de) |
| EP (1) | EP0380495B1 (de) |
| JP (1) | JP2898291B2 (de) |
| AT (1) | ATE105856T1 (de) |
| AU (1) | AU2126688A (de) |
| CA (1) | CA1309679C (de) |
| DE (1) | DE3889665T2 (de) |
| IE (1) | IE64262B1 (de) |
| IL (1) | IL87196A (de) |
| WO (1) | WO1989001028A1 (de) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5763573A (en) * | 1988-08-10 | 1998-06-09 | Chiron Corporation | GTPase activating protein fragments |
| US5760203A (en) * | 1988-08-10 | 1998-06-02 | Chiron Corporation | Gap gene sequences |
| US5272063A (en) * | 1988-11-22 | 1993-12-21 | Syntex (U.S.A.) Inc. | Process of making human nerve growth factor |
| ES2112866T3 (es) * | 1990-09-25 | 1998-04-16 | Smithkline Beecham Corp | Medios para el cultivo de celulas de drosophila. |
| CA2091443A1 (en) * | 1990-09-25 | 1992-03-26 | Luciano Ramos | Medium for culture of mammalian cells |
| JPH04228066A (ja) * | 1990-10-23 | 1992-08-18 | Rikagaku Kenkyusho | 外来遺伝子発現用培養細胞 |
| JPH0671432B2 (ja) * | 1991-03-20 | 1994-09-14 | 株式会社ミドリ十字 | ヒト血清アルブミンの製造方法 |
| US5318898A (en) * | 1991-04-02 | 1994-06-07 | Genetics Institute, Inc. | Production of recombinant bone-inducing proteins |
| KR100270195B1 (ko) * | 1991-09-06 | 2001-03-02 | 와일러 제임스 에프. | 겔로닌 폴리펩타이드를 암호화하는 dna 서열 |
| US5298418A (en) * | 1991-09-16 | 1994-03-29 | Boyce Thompson Institute For Plant Research, Inc. | Cell line isolated from larval midgut tissue of Trichoplusia ni |
| US5405770A (en) * | 1993-01-21 | 1995-04-11 | The United States Of America As Represented By The Secretary Of Agriculture | Heliothis subflexa cell line for the production of baculoviruses |
| US5348877A (en) * | 1993-03-12 | 1994-09-20 | Boyce Thompson Institute For Plant Research, Inc. | Method of adapting anchorage-dependent cell lines to suspension conditions |
| WO1994026087A2 (en) * | 1993-05-14 | 1994-11-24 | Connor Kim C O | Recombinant protein production and insect cell culture and process |
| US5550019A (en) | 1993-05-26 | 1996-08-27 | La Jolla Cancer Research Foundation | Methods of identifying compounds which alter apoptosis |
| US5426045A (en) * | 1993-12-16 | 1995-06-20 | Sea Run Holdings, Inc. | Method for culturing mammalian cells in a medium containing fish serum |
| US5641678A (en) * | 1994-01-18 | 1997-06-24 | Smithkline Beecham Corporation | Serum-free culture medium for drosphila insect cells |
| US5498540A (en) * | 1994-02-23 | 1996-03-12 | Sea Run Holdings, Inc. | Method for culturing insect cells in a medium containing fish serum |
| US6403375B1 (en) * | 1994-08-24 | 2002-06-11 | Boyce Thompson Institute For Plant Research, Inc. | Establishment of Trichoplusia ni cell lines in serum-free medium for recombinant protein and baculovirus production |
| FR2726574B1 (fr) * | 1994-11-04 | 1997-01-17 | Univ Paris Curie | Procede de dissociation des cellules eucaryotes en culture |
| US5728580A (en) * | 1996-02-20 | 1998-03-17 | Cornell Research Foundation, Inc. | Methods and culture media for inducing single cell suspension in insect cell lines |
| DK1975177T3 (da) | 1996-03-01 | 2011-07-25 | Novo Nordisk As | Appetitundertrykkende peptid, formuleringer dermed og anvendelse deraf |
| JP2000507812A (ja) * | 1996-03-12 | 2000-06-27 | ライフ テクノロジーズ，インコーポレイテッド | 造血細胞培養栄養補充成分 |
| EP2243827B2 (de) * | 1996-08-30 | 2017-11-22 | Life Technologies Corporation | Serumfreies kulturmedium für säugerzellen und seine verwendung |
| CA2286395C (en) | 1997-04-03 | 2009-06-23 | Yoshitomi Pharmaceutical Industries Ltd. | Production method of heterologous protein |
| US5804420A (en) * | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
| US6066602A (en) * | 1997-08-07 | 2000-05-23 | Becton Dickinson And Company | Waterborne non-silicone lubricant comprising phospholipid and polyether |
| US6051401A (en) * | 1998-07-28 | 2000-04-18 | Bayer Corporation | Methods and constructs for protein expression |
| AT409379B (de) * | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| US6670184B2 (en) * | 2000-06-23 | 2003-12-30 | Basf Ag | Method for producing a lipid emulsion for use in insect cell culture |
| DE10050091A1 (de) * | 2000-10-09 | 2002-04-25 | Biotechnolog Forschung Gmbh | Casein-Peptidfragmente mit wachstumsbeeinflußender Aktivität auf Zellkulturen |
| US7445924B2 (en) * | 2000-11-23 | 2008-11-04 | Bavarian Nordic A/S | Modified Vaccinia Ankara virus variant and cultivation method |
| IL157842A0 (en) | 2001-03-22 | 2004-03-28 | Novo Nordisk Healthcare Ag | Coagulation factor vii derivatives |
| WO2002090533A2 (en) * | 2001-05-07 | 2002-11-14 | National Research Council Of Canada | Enhanced production of recombinant proteins by transient transfection of suspension-growing mammalian cells |
| GB0123098D0 (en) | 2001-09-26 | 2001-11-14 | Lonza Biologics Plc | Use of aminoglycoside resistance gene |
| US6943023B2 (en) * | 2002-03-06 | 2005-09-13 | National Institute Of Agrobiological Sciences | Insect cell primary culture medium, extra cellular matrix, and process of preparing an insect culture cell line in a short period of time using the medium and matrix |
| AU2003264144C1 (en) | 2002-09-05 | 2009-12-17 | Bavarian Nordic A/S | Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions |
| EP2392655A3 (de) | 2003-09-09 | 2012-02-29 | Novo Nordisk Health Care AG | Gerinnungsfaktor VII Polypeptide |
| US7087369B2 (en) * | 2003-12-17 | 2006-08-08 | The Regents Of The University Of California | Cornea preservation medium |
| EP2305821A3 (de) | 2004-02-13 | 2011-04-13 | Novozymes A/S | Proteasevarianten |
| US7422875B2 (en) * | 2004-07-20 | 2008-09-09 | Board Of Regents Of The University Of Nebraska | Compositions and methods for increasing protein production |
| MX2007012887A (es) | 2005-04-18 | 2007-12-10 | Novo Nordisk As | Variantes il-21. |
| WO2007031559A2 (en) | 2005-09-14 | 2007-03-22 | Novo Nordisk Health Care Ag | Human coagulation factor vii polypeptides |
| CN105219734B (zh) | 2008-12-05 | 2020-03-03 | 武田疫苗公司 | 用于诱导病毒生长的组合物、方法和用途 |
| WO2011006982A2 (en) | 2009-07-17 | 2011-01-20 | Rigshospitalet | Inhibitors of complement activation |
| US9815876B2 (en) | 2010-03-05 | 2017-11-14 | Omeros Corporation | Chimeric inhibitor molecules of complement activation |
| JP6517018B2 (ja) | 2012-02-09 | 2019-05-22 | ヴァー２・ファーマシューティカルズ・アンパルトセルスカブＶＡＲ２ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ ＡｐＳ | コンドロイチン硫酸グリカンのターゲティング |
| JP2015532307A (ja) | 2012-10-15 | 2015-11-09 | ノヴォ・ノルディスク・ヘルス・ケア・アーゲー | 凝固因子ｖｉｉポリペプチド |
| EP2970916B1 (de) | 2013-03-13 | 2021-04-14 | Merck Sharp & Dohme Corp. | Adaptierte lepidoptera-insektenzellen zur produktion rekombinanter proteine |
| HUE052232T2 (hu) | 2013-05-06 | 2021-04-28 | Scholar Rock Inc | Készítmények és eljárások növekedési faktor modulálására |
| AU2014329403B2 (en) | 2013-10-03 | 2020-10-08 | Takeda Vaccines, Inc. | Methods of detection and removal of rhabdoviruses from cell lines |
| CN105441376B (zh) * | 2015-12-22 | 2018-03-20 | 肇庆大华农生物药品有限公司 | 一种用于培养病毒的无血清培养基及其制备方法 |
| WO2017195745A1 (ja) * | 2016-05-09 | 2017-11-16 | 国立研究開発法人医薬基盤・健康・栄養研究所 | 培地、アルブミン非含有培地用添加剤および多能性幹細胞の培養方法 |
| US20210278406A1 (en) | 2018-07-13 | 2021-09-09 | Varct Diagnostic Aps | Isolation of circulating cells of fetal origin using recombinant malaria protein var2csa |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US30985A (en) * | 1860-12-18 | Thomas l | ||
| USRE30985E (en) * | 1978-01-01 | 1982-06-29 | Serum-free cell culture media | |
| DE3048289A1 (de) * | 1980-12-20 | 1982-07-29 | Hoechst Ag | "verfahren zur zuechtung von insektenzellen" |
| JPS5831989A (ja) * | 1981-08-13 | 1983-02-24 | Asahi Chem Ind Co Ltd | 正常二倍体細胞の培養方法 |
| DE3216163A1 (de) * | 1982-04-30 | 1983-11-03 | Behringwerke Ag | Kulturmedium und verfahren zur virusvermehrung in suspensionskultur |
| ATE78293T1 (de) * | 1983-05-27 | 1992-08-15 | Texas A & M Univ Sys | Verfahren zur herstellung eines rekombinanten baculovirus-expressionsvektors. |
| DK518384A (da) * | 1984-01-31 | 1985-07-01 | Idaho Res Found | Vektor til fremstilling af et gen-produkt i insektceller, fremgangsmaade til dens fremstilling samt dens anvendelse |
| US4485029A (en) * | 1984-03-19 | 1984-11-27 | Minnesota Mining And Manufacturing Company | Disinfecting method and compositions |
| US4752585A (en) * | 1985-12-17 | 1988-06-21 | Cetus Corporation | Oxidation-resistant muteins |
| JPH0615447B2 (ja) * | 1985-12-02 | 1994-03-02 | 進 前田 | ウイルスを利用した殺虫剤及びその調製法 |
-
1987
- 1987-07-24 US US07/077,303 patent/US5024947A/en not_active Expired - Fee Related
-
1988
- 1988-07-20 EP EP88906600A patent/EP0380495B1/de not_active Expired - Lifetime
- 1988-07-20 AT AT88906600T patent/ATE105856T1/de not_active IP Right Cessation
- 1988-07-20 WO PCT/US1988/002443 patent/WO1989001028A1/en not_active Ceased
- 1988-07-20 AU AU21266/88A patent/AU2126688A/en not_active Abandoned
- 1988-07-20 CA CA000572548A patent/CA1309679C/en not_active Expired - Fee Related
- 1988-07-20 DE DE3889665T patent/DE3889665T2/de not_active Expired - Fee Related
- 1988-07-20 JP JP63506450A patent/JP2898291B2/ja not_active Expired - Lifetime
- 1988-07-22 IL IL87196A patent/IL87196A/xx not_active IP Right Cessation
- 1988-07-22 IE IE226488A patent/IE64262B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| IE882264L (en) | 1989-01-24 |
| JPH03500602A (ja) | 1991-02-14 |
| IL87196A (en) | 1993-04-04 |
| IL87196A0 (en) | 1988-12-30 |
| EP0380495B1 (de) | 1994-05-18 |
| CA1309679C (en) | 1992-11-03 |
| US5024947A (en) | 1991-06-18 |
| WO1989001028A1 (en) | 1989-02-09 |
| IE64262B1 (en) | 1995-07-26 |
| EP0380495A1 (de) | 1990-08-08 |
| DE3889665D1 (de) | 1994-06-23 |
| AU2126688A (en) | 1989-03-01 |
| DE3889665T2 (de) | 1994-11-03 |
| JP2898291B2 (ja) | 1999-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE3889665D1 (de) | Serumfreie wachstumsmedien für insektenzellen und expression von produkten damit. | |
| GB2091271B (en) | Recombinant dna | |
| DK112686A (da) | Fremgangsmaade og apparat til dyrkning af cellekulturer | |
| DE69023873D1 (de) | Statischer oxygenator zur suspensionskultur von tierischen zellen. | |
| IL68366A (en) | Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture | |
| KR880007726A (ko) | 안정하게 형질감염된 포유동물 세포에서 사람 에리트로포이에틴 유전자를 고농도 형질발현시키는 방법 | |
| DE3851035D1 (de) | Interleukin-7. | |
| DE69033961D1 (de) | Zellinien, die einen biologisch aktiven faktor-ix exprimieren | |
| DE3769872D1 (de) | Herstellung von rekombinantem protein. | |
| RU94043769A (ru) | Способ повышения продукции протеина с, способ повышения уровня его модификации | |
| IL77049A0 (en) | Isolation and culture of adrenal medullary endothelial cells producing blood clotting factor viii:c | |
| CA1312566C (en) | Method for increasing the expression of polypeptides in recombinant cell culture | |
| ATE97958T1 (de) | Zellenzuchtverfahren fuer die produktion von aktiviertem protein-c. | |
| AU581819B2 (en) | Process for the immortalization of human and animal cells | |
| ATE67782T1 (de) | Cholesterolreiche fraktion, verfahren dafuer und verwendung derselben zur zellkultur. | |
| ATE288476T1 (de) | Herstellung von proteinen | |
| DE69014723D1 (de) | Zellzüchtungsverfahren. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EEIH | Change in the person of patent owner | ||
| REN | Ceased due to non-payment of the annual fee |