ATE159984T1 - Nachweis von nukleinsäuresequenzen unter verwendung von fluoreszenz-polarisation - Google Patents

Nachweis von nukleinsäuresequenzen unter verwendung von fluoreszenz-polarisation

Info

Publication number
ATE159984T1
ATE159984T1 AT90301135T AT90301135T ATE159984T1 AT E159984 T1 ATE159984 T1 AT E159984T1 AT 90301135 T AT90301135 T AT 90301135T AT 90301135 T AT90301135 T AT 90301135T AT E159984 T1 ATE159984 T1 AT E159984T1
Authority
AT
Austria
Prior art keywords
nucleic acid
detection
acid sequences
fluorescence polarization
detect
Prior art date
Application number
AT90301135T
Other languages
English (en)
Inventor
Andrew John Garman
Robert Stanley Moore
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Application granted granted Critical
Publication of ATE159984T1 publication Critical patent/ATE159984T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Hybrid Electric Vehicles (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Surgical Instruments (AREA)
  • Transducers For Ultrasonic Waves (AREA)
AT90301135T 1989-02-07 1990-02-02 Nachweis von nukleinsäuresequenzen unter verwendung von fluoreszenz-polarisation ATE159984T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898902689A GB8902689D0 (en) 1989-02-07 1989-02-07 Assay method

Publications (1)

Publication Number Publication Date
ATE159984T1 true ATE159984T1 (de) 1997-11-15

Family

ID=10651255

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90301135T ATE159984T1 (de) 1989-02-07 1990-02-02 Nachweis von nukleinsäuresequenzen unter verwendung von fluoreszenz-polarisation

Country Status (14)

Country Link
US (1) US6022686A (de)
EP (1) EP0382433B1 (de)
JP (1) JPH02295496A (de)
AT (1) ATE159984T1 (de)
AU (1) AU4902590A (de)
CA (1) CA2009454C (de)
DE (1) DE69031665T2 (de)
ES (1) ES2109226T3 (de)
FI (1) FI900602A7 (de)
GB (2) GB8902689D0 (de)
IE (1) IE900442L (de)
IL (1) IL93297A0 (de)
NO (1) NO900582L (de)
ZA (1) ZA90912B (de)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US6007984A (en) * 1989-11-01 1999-12-28 Zeneca Limited Detection of DNA/RNA by fluorescence polarization
US5453355A (en) * 1990-01-26 1995-09-26 Abbott Laboratories Oligonucleotides and methods for the detection of Neisseria gonorrhoeae
US5641629A (en) * 1990-06-11 1997-06-24 Nexstar Pharmacueticals Inc Spectroscopically detectable nucleic acid ligands
CA2091764C (en) * 1990-09-20 2003-04-08 Michael Louis Bittner Probe compositions for chromosome identification and methods
US5620847A (en) * 1990-10-05 1997-04-15 Hoffman-La Roche Inc. Methods and reagents for detection of bacteria in cerebrospinal fluid
WO1992018650A1 (en) * 1991-04-11 1992-10-29 Baxter Diagnostics Inc. Detection of dna/rna by fluorescence polarization
GB9119735D0 (en) * 1991-09-16 1991-10-30 Secr Defence Gene probe biosensor method
US6033854A (en) * 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US5567583A (en) * 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
US5348853A (en) * 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
EP0599337B1 (de) * 1992-11-27 2006-03-08 Canon Kabushiki Kaisha Verfahren und Sonde zum Nachweis von Nukleinsäuren
JPH07177899A (ja) * 1993-12-24 1995-07-18 Bio Sensor Kenkyusho:Kk 遺伝子の検出方法
JPH07233065A (ja) * 1993-12-27 1995-09-05 Canon Inc ピリリウム塩又はピリリウム類似塩を含む光化学治療薬
CA2145719C (en) * 1994-04-18 1998-06-30 James G. Nadeau Detection of nucleic acid amplification
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
DE69532255D1 (de) * 1994-05-26 2004-01-22 Canon Kk Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung
AU705637B2 (en) * 1994-09-23 1999-05-27 Becton Dickinson & Company Detection of nucleic acid amplification
GB9510262D0 (en) * 1995-05-22 1995-07-19 Nat Blood Authority The Detection method
EP0790861A1 (de) * 1995-09-12 1997-08-27 Becton, Dickinson and Company Verfahren und vorrichtung zur dns-amplifikation und test
WO1997014028A2 (en) * 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US5800989A (en) * 1995-11-15 1998-09-01 Becton, Dickinson And Company Method for detection of nucleic acid targets by amplification and fluorescence polarization
US5641633A (en) * 1995-11-15 1997-06-24 Becton, Dickinson And Company Fluorescence polarization detection of nucleic acids
CA2190430A1 (en) * 1995-12-12 1997-06-13 Margret Barbara Basinski Method for measuring genetic messages
US5723294A (en) * 1996-03-05 1998-03-03 Gull Laboratories Methods for detection and discrimination of multiple analytes using fluorescent technology
ATE230801T1 (de) 1996-10-03 2003-01-15 Canon Kk Verfahren zur detektion von zielnukleinsäure, verfahren zu ihrer quantifizierung und pyrylium- verbindungen zur chemilumineszenz-analyse
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6503709B1 (en) 1997-07-03 2003-01-07 Id Biomedical Corporation Methods for rapidly detecting methicillin resistant staphylococci
US6013442A (en) * 1997-08-05 2000-01-11 Wisconsin Alumni Res Found Direct quantitation of low copy number RNA
US6261781B1 (en) 1997-08-05 2001-07-17 Wisconsin Alumni Research Foundation Direct detection and mutation analysis of low copy number nucleic acids
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
WO1999060158A1 (en) * 1998-05-19 1999-11-25 Laboratory Of Molecular Biophotonics Solid phase for detecting nucleic acid and method for detecting nucleic acid
JP3437094B2 (ja) * 1998-07-03 2003-08-18 松下電器産業株式会社 多波長蛍光偏光法
US6180408B1 (en) * 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
FR2784748A1 (fr) * 1998-10-19 2000-04-21 Revelgenics Detection de reactions de polymerisation par polarisation de fluorescence
US6100039A (en) * 1999-01-28 2000-08-08 Panvera, Inc. Process for reverse transcriptase activity measurement using fluorescence polarization
JP2003527075A (ja) 1999-03-25 2003-09-16 ハイセック,インコーポレーテッド ハイブリダイゼーションによる配列分析のための、溶液ベースの方法
AU6152301A (en) * 2000-05-12 2001-11-26 Caliper Techn Corp Detection of nucleic acid hybridization by fluorescence polarization
US6977161B2 (en) 2000-10-14 2005-12-20 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
CA2409309A1 (en) 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
US6258546B1 (en) * 2000-06-23 2001-07-10 Becton, Dickinson And Company Detection of nucleic acid amplification
WO2002046453A2 (en) * 2000-12-05 2002-06-13 Syngenta Participations Ag Method and kit for identification of nucleic acid modification enzymes and inhibitors thereof
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
DE10104937B4 (de) * 2001-01-29 2005-03-17 Epigenomics Ag Fluoreszenzpolarisation 2
US8148171B2 (en) 2001-10-09 2012-04-03 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6589744B2 (en) 2001-11-26 2003-07-08 Syngenta Participations Ag Method and kit for identification for nucleic acid modification enzymes and inhibitors thereof
CA2498713A1 (en) * 2002-09-17 2004-04-01 Richard Abraham Joseph Real-time detection of nucleic acid reactions
DE602004018270D1 (de) 2003-01-17 2009-01-22 Eragen Biosciences Inc Nukleinsäureamplifikation mit nichtstandardbasen
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
JP4567673B2 (ja) 2003-04-01 2010-10-20 エラジェン バイオサイエンシズ インコーポレイテッド ポリメラーゼ阻害剤およびその使用方法
GB0317592D0 (en) 2003-07-26 2003-08-27 Astrazeneca Ab Method
US20070128608A1 (en) * 2003-12-19 2007-06-07 Kankyo Engineering Co., Ltd. Novel mixtures for assaying nucleic acid, novel method of assaying nucleic acid with the use of the same and nucleic acid probe to be used therefor
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US7851152B2 (en) * 2004-09-25 2010-12-14 Yaodong Chen Fluorescent base analogues' usage in the characterization of nucleic acid molecules and their interactions
US20060172314A1 (en) * 2005-01-31 2006-08-03 Song Min-Sun Quantification of amplified nucleic acids
WO2006101913A2 (en) 2005-03-18 2006-09-28 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of neiserria
WO2006112141A1 (ja) * 2005-03-30 2006-10-26 Toyo Boseki Kabushiki Kaisha 塩基判定方法及び塩基判定用キット
CA2610863C (en) 2005-06-07 2017-01-17 Eragen Biosciences, Inc. Methods for detection and typing of nucleic acids
JP2007330134A (ja) * 2006-06-14 2007-12-27 Toyobo Co Ltd 核酸の検出方法
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
WO2008157649A1 (en) * 2007-06-18 2008-12-24 Northwestern University Non-enzymatic detection of bacterial genomic dna
WO2010112033A2 (en) 2009-03-31 2010-10-07 Østjysk Innovation A/S Method for estimating the risk of having or developing multiple sclerosis using sequence polymorphisms in a specific region of chromosome x
CN102639712A (zh) 2009-10-23 2012-08-15 卢米耐克斯公司 用于提高反应特异性的具有非标准碱基的扩增引物
WO2012052758A1 (en) 2010-10-22 2012-04-26 Astrazeneca Ab Response biomarkers for iap antagonists in human cancers
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
JP2016509480A (ja) 2013-01-24 2016-03-31 カリフォルニア インスティチュート オブ テクノロジー 発色団に基づく特徴づけおよび検出方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
US4668640A (en) * 1981-12-11 1987-05-26 Abbott Laboratories Fluorescence polarization immunoassay utilizing substituted carboxyfluoresceins
US4476228A (en) * 1982-11-08 1984-10-09 Abbott Laboratories Determination of unsaturated thyroxine binding protein sites using fluorescence polarization techniques
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4670380A (en) * 1984-05-23 1987-06-02 Molecular Diagnostics, Inc. Assays utilizing labeled nucleic acid probes
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
ES8706823A1 (es) * 1985-03-28 1987-06-16 Cetus Corp Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
JPS61258168A (ja) * 1985-05-13 1986-11-15 Kogyo Kaihatsu Kenkyusho 核酸配列を同定する方法
US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
EP0232967B1 (de) * 1986-01-10 1993-04-28 Amoco Corporation Kompetitiver homogener Test
JPH02502122A (ja) * 1987-01-15 1990-07-12 ヘベイ,リチヤード・シー 液体中のリガンドを検出及び定量する方法
US4863876A (en) * 1987-01-15 1989-09-05 Hevey Richard C Method of detecting and quantifying ligands in liquids via biotin-avidin-medicated fluorescence polarization
CA1317535C (en) * 1987-06-30 1993-05-11 Nanibhushan Dattagupta Assay of sequences using amplified genes
PT88665B (pt) * 1987-10-05 1992-12-31 Ml Tecnology Ventures Lp Metodo para a marcacao com ester de acridinio e purificacao de sondas nucleotidicas
US5185439A (en) * 1987-10-05 1993-02-09 Gen-Probe Incorporated Acridinium ester labelling and purification of nucleotide probes
AU622426B2 (en) * 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
JPH0975958A (ja) * 1995-09-06 1997-03-25 Daiei Kogyo Kk オゾン水吐出装置

Also Published As

Publication number Publication date
GB2228998B (en) 1993-04-07
EP0382433A2 (de) 1990-08-16
GB8902689D0 (en) 1989-03-30
DE69031665T2 (de) 1998-02-26
US6022686A (en) 2000-02-08
GB2228998A (en) 1990-09-12
IE900442L (en) 1990-08-07
EP0382433B1 (de) 1997-11-05
CA2009454A1 (en) 1990-08-07
NO900582D0 (no) 1990-02-07
DE69031665D1 (de) 1997-12-11
FI900602A0 (fi) 1990-02-07
FI900602A7 (fi) 1990-08-08
EP0382433A3 (de) 1992-01-15
JPH02295496A (ja) 1990-12-06
CA2009454C (en) 2000-04-04
GB9002536D0 (en) 1990-04-04
ZA90912B (en) 1990-10-31
IL93297A0 (en) 1990-11-29
NO900582L (no) 1990-08-08
ES2109226T3 (es) 1998-01-16
AU4902590A (en) 1990-08-16

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