ATE171707T1 - Proteinherstellung - Google Patents

Proteinherstellung

Info

Publication number
ATE171707T1
ATE171707T1 AT93901661T AT93901661T ATE171707T1 AT E171707 T1 ATE171707 T1 AT E171707T1 AT 93901661 T AT93901661 T AT 93901661T AT 93901661 T AT93901661 T AT 93901661T AT E171707 T1 ATE171707 T1 AT E171707T1
Authority
AT
Austria
Prior art keywords
protein
production
protein production
continuously
inactivation
Prior art date
Application number
AT93901661T
Other languages
English (en)
Inventor
Per Linaa Joergensen
Poul Erik Pedersen
Joergen Petersen
Torben K Nielsen
Jan Moeller Mikkelsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE171707T1 publication Critical patent/ATE171707T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT93901661T 1991-12-16 1992-12-11 Proteinherstellung ATE171707T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK9100394 1991-12-16
US07/848,507 US5371198A (en) 1991-12-16 1992-03-09 Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
PCT/DK1992/000374 WO1993013125A1 (en) 1991-12-16 1992-12-11 Protein production

Publications (1)

Publication Number Publication Date
ATE171707T1 true ATE171707T1 (de) 1998-10-15

Family

ID=26068345

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93901661T ATE171707T1 (de) 1991-12-16 1992-12-11 Proteinherstellung

Country Status (9)

Country Link
US (1) US5371198A (de)
EP (1) EP0631585B1 (de)
JP (1) JPH07503458A (de)
AT (1) ATE171707T1 (de)
CA (1) CA2125853A1 (de)
DE (1) DE69227200T2 (de)
ES (1) ES2124782T3 (de)
FI (1) FI942835A7 (de)
WO (1) WO1993013125A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5623059A (en) * 1992-03-09 1997-04-22 Novo Nordisk A/S Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
US5405767A (en) * 1992-04-08 1995-04-11 Solvay Enzymes, Inc. Purified enzyme concentrate and method of preparation
US5576283A (en) * 1992-08-14 1996-11-19 The Procter & Gamble Company Liquid detergents containing a peptide aldehyde
US5582762A (en) * 1992-08-14 1996-12-10 The Procter & Gamble Company Liquid detergents containing a peptide trifluoromethyl ketone
WO1995025791A1 (en) * 1994-03-22 1995-09-28 The Procter & Gamble Company Protease enzyme manufacture using non-protein protease inhibitors
JP2000506145A (ja) * 1996-03-08 2000-05-23 ノボ ノルディスク アクティーゼルスカブ 硫化塩によるタンパク質の結晶化
WO1998013483A1 (en) * 1996-09-24 1998-04-02 The Procter & Gamble Company Proteases and their variants having peptide protease inhibitors fused to them
CA2266323A1 (en) 1996-09-24 1998-04-02 The Procter & Gamble Company Stabilized proteinaceous protease inhibitors and variants thereof
CN1113088C (zh) * 1996-09-24 2003-07-02 普罗格特-甘布尔公司 含有蛋白酶和蛋白酶抑制剂的液体洗涤剂
US6165966A (en) * 1996-09-24 2000-12-26 The Procter & Gamble Company Liquid detergents containing proteolytic enzyme and protease inhibitors
CA2266525A1 (en) 1996-09-24 1998-04-02 Charles Winston Saunders Liquid laundry detergent compositions containing proteolytic enzyme and protease inhibitors
US20040038845A1 (en) * 2000-08-21 2004-02-26 Pedersen Poul Erik Method for production of a protease-inhibitor complex
US20060127974A1 (en) * 2002-07-01 2006-06-15 Novozymes A/S Mpg added to fermentation
US8956679B2 (en) 2006-03-20 2015-02-17 Corn Products Development Inc. Process tolerant starch composition with high total dietary fiber content
ATE498007T1 (de) * 2007-08-20 2011-02-15 Novozymes As Nukleasereduktion
AR076288A1 (es) * 2009-04-13 2011-06-01 Bristol Myers Squibb Co Purificacion de proteinas por precipitacion con citrato
KR102307278B1 (ko) * 2014-04-29 2021-09-30 노파르티스 아게 신규 척추동물 세포 및 관심 폴리펩티드의 재조합적 발현 방법
US20200109388A1 (en) 2017-04-03 2020-04-09 Novozymes A/S Recovery Process
CN107266548A (zh) * 2017-07-28 2017-10-20 浙江理工大学 一种利用离子液体和蛋白酶提取柞蚕丝丝素蛋白的方法
JP6991312B2 (ja) * 2018-03-26 2022-01-12 次世代バイオ医薬品製造技術研究組合 インラインミキサーとハイドロサイクロンを統合したモジュールを複数用いて抗体を細胞培養液から連続的に製造する装置および当該装置を用いた抗体の製造方法
CN113166231A (zh) 2018-10-10 2021-07-23 诺维信公司 胰凝乳蛋白酶抑制剂变体及其用途
US20220186234A1 (en) 2019-02-20 2022-06-16 Basf Se Industrial Fermentation Process for Bacillus Using Defined Medium and Trace Element Feed
EP3927837A1 (de) 2019-02-20 2021-12-29 Basf Se Industrielles fermentationsverfahren für bacillus unter verwendung eines definierten mediums und einer magnesiumzufuhr
US20260062690A1 (en) 2022-08-02 2026-03-05 Basf Se Stabilized protein production process using bacillus host cells
WO2025094385A1 (ja) * 2023-11-02 2025-05-08 株式会社ノリタケカンパニーリミテド タンパク質精製装置および精製タンパク質の製造方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2512735A1 (de) * 1975-03-22 1976-09-30 Henkel & Cie Gmbh Verfahren zur gewinnung von proteinen aus waessrigen protein- loesungen
JPS5411294A (en) * 1977-05-23 1979-01-27 Toyo Soda Mfg Co Ltd Recovery of protease
DK108685A (da) * 1984-03-19 1985-09-20 Fujisawa Pharmaceutical Co Vaekstfaktor i
US4673647A (en) * 1985-05-06 1987-06-16 Miles Laboratories, Inc. Process to solubilize enzymes and an enzyme liquid product produced thereby
WO1987004187A1 (en) * 1986-01-03 1987-07-16 Genetics Institute, Inc. METHOD FOR PRODUCING FACTOR VIII:c-TYPE PROTEINS
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
WO1990002175A1 (en) * 1988-08-16 1990-03-08 Novo Nordisk A/S A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
AU624969B2 (en) * 1989-06-13 1992-06-25 Chr. Hansen A/S Processes for the recovery of naturally produced chymosin
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen

Also Published As

Publication number Publication date
EP0631585B1 (de) 1998-09-30
ES2124782T3 (es) 1999-02-16
FI942835L (fi) 1994-06-15
US5371198A (en) 1994-12-06
WO1993013125A1 (en) 1993-07-08
DE69227200T2 (de) 1999-05-20
FI942835A0 (fi) 1994-06-15
JPH07503458A (ja) 1995-04-13
DE69227200D1 (de) 1998-11-05
CA2125853A1 (en) 1993-07-08
FI942835A7 (fi) 1994-06-15
EP0631585A1 (de) 1995-01-04

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Legal Events

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